Identification and overexpression of the A2 amastigote-specific protein in Leishmania donovani

Molecular and Biochemical Parasitology

Volumn 78, Issue 1-2, 1996, Pages 79-90

  Zhang, Wen Wei ^b   Charest, Hugues ^a,b   Ghedin, Elodie ^b   Matlashewski, Greg ^b  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b MCGILL UNIVERSITY   (Canada)

Author keywords

Amastigote specific protein; Gene expression; L. donovani

Indexed keywords

PARASITE ANTIGEN; RECOMBINANT PROTEIN A2; UNCLASSIFIED DRUG;

AMASTIGOTE; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL DIFFERENTIATION; HOST PARASITE INTERACTION; LEISHMANIA DONOVANI; MOUSE; NONHUMAN; PARASITE SURVIVAL; PRIORITY JOURNAL; PROMASTIGOTE; PROTEIN EXPRESSION;

ANIMALIA; ESCHERICHIA COLI; LEISHMANIA DONOVANI; MAMMALIA; PROTOZOA;

EID: 0030014943     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(96)02612-6     Document Type: Article

References (20)

1. Moody, S. (1993) Molecular variations in Leishmania. Acta Tropica 53, 185-204.
2. Charest, H. and Matlashewski, G. (1994) Developmental gene expression in Leishmania donovani: differential cloning and analysis of the amastigote-stage-specific gene. Mol. Cell. Biol. 14, 2957-2984.
3. Charest, H., Zhang, W.W. and Matlashewski, G. (Submitted) The developmental expression of Leishmania donovani A2 amastigote-specific genes is post-transcriptionally mediated and involves elements located in the 3′ UTR.
4. Sambrooke, J., Fritsch, F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
5. Harlow, E. and Lane, D. (1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Press, Cold Spring Harbor, N.Y.
6. Zhang, W.W. and Samuelson, J. (1993) The cDNA sequence of an abundant Entamoeba histolytica 20-kilodalton protein containing four repetitive domains. Mol. Biochem. Parasitol. 60, 323-326.
7. Smith, D.B. and Johnson, K.S. (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40.
8. Zhang, W.W., Charest, H. and Matlashewski, G. (1995) The expression of biologically active human p53 in Leishmania cells: a novel eukaryotic system to produce recombinant protein. Nucleic Acids Res. 23, 4073-4080.
9. Papadopoulou, B., Roy, G. and Ouellette, M. (1994) Autonomous replication of bacterial DNA plasmid oligomers in Leishmania. Mol. Biochem. Parasitol. 65, 39-49.
10. Γ gene in L. Enriettii. Nature 343, 572-574.
11. Souza, A., Waugh, S., Coombs, G. and Mottram, J. (1992) Characterisation of a multicopy gene for a major stage specific cysteine proteinase of Leishmania mexicana. FEBS Lett. 311, 124-127.
12. Mottram, J., Roberston, C., Coombs, G. and Barry, D. (1992) A developmentally regulated cysteine proteinase gene of Leishmania mexicana. Mol. Microbiol. 6, 1925-1932.
13. Traub-Cseko, Y., Duboise, M., Boukai, L. and McMahon-Pratt, D. (1993) Identification of two distinct cysteine proteinase genes of Leishmania pifanoi axenic amastigotes using the polymerase chain reaction. Mol. Biochem. Parasitol. 57, 101-116.
14. Souza, A., Bates, P., Coombs, G. and Mottram, J. (1994) Null mutants for the lmcpa cysteine proteinase gene in Leishmania mexicana. Mol. Biochem. Parasitol. 63, 213-220.
15. Kidane, G., Samaras, N. and Spithill, T. (1989) Cloning of developmentally regulated genes from Leishmania major and expression following heat induction. J. Biol. Chem. 264, 4244-4250.
16. Pan, A. and McMahon-Pratt, D. (1988) Monoclonal antibodies specific for the amastigote stage of Leishmania pifanoi. Characterization of antigens associated with stage-and species-specific determinants. J. Immunol. 140, 2406-2414.
17. Straus, A., Levery, S., Jasiulionis, M., Salyan, M.E., Steele, S., Travassos, L., Hakomori, S.-I. and Takahashi, H. (1993) Stage-specific glycospingolipids from amastigotes forms of Leishmania (L.) amazonensis. J. Biol. Chem. 268, 13723-13730.
18. Burns, J., Shreffler, W., Benson, D., Ghalib, H., Badaro, R. and Reed, S. (1993) Molecular characterization of a kinesin-related antigen of Leishmania chagasi that detects specific antibody in African and American visceral leishmaniasis. Proc. Natl. Acad. Sci. 90, 775-779.
19. Rey-Lading, J. and Reiner, N. (1993) Expression of 65-and 67-kilodalton heat-regulated proteins and a 70-kilodalton heat shock cognate protein of Leishmania donovani in macrophages. Infect. Immunol. 61, 3265-3272.
20. Brandau, S., Dresel, A. and Clos, J. (1995) High constitutive levels of heat-shock proteins in human-pathogenic parasites of the genus Leishmania. Biochem J. 310, 225-232.