Cloning and functional expression of the mouse epithelial sodium channel

American Journal of Physiology - Renal Physiology

Volumn 277, Issue 1 46-1, 1999, Pages

  Ahn, Yoon J ^a   Brooker, David R ^a   Kosari, Farhad ^a   Harte, Brian J ^a   Li, Jinqing ^a   Mackler, Scott A ^a   Kleyman, Thomas R ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Cloning; Structure function relationship; Xenopus oocytes

Indexed keywords

AMILORIDE; AMINO ACID; COMPLEMENTARY DNA; MESSENGER RNA; RNA; SODIUM; SODIUM CHANNEL;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; EPITHELIUM; KIDNEY; LIVER; LUNG; MOLECULAR CLONING; MOUSE; NONHUMAN; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION; TISSUE DISTRIBUTION; XENOPUS;

EID: 0032775101     PISSN: 1931857X     EISSN: 15221466     Source Type: Journal    
DOI: 10.1152/ajprenal.1999.277.1.f121     Document Type: Article

References (61)

1. Adams, C., P. Snyder, and M. Welsh. Interactions between subunits of the human epithelial sodium channel. J. Biol. Chem. 272: 27295-27300, 1997.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. Current Protocols in Molecular Biology. New York: Wiley, 1995.
3. Awayda, M., and M. Subramanyam. Regulation of the epithelial Na channel by membrane tension. J. Gen. Physiol. 112: 97-111, 1998.
4. + channel protein displays stretch activation in planar lipid bilayers. Am. J. Physiol. 268 (Cell Physiol. 37): C1450-C1459, 1995.
5. + channels. J. Membr. Biol. 143: 1-18, 1995.
6. Brooker, D. R., C. A. Kozak, and T. R. Kleyman. Epithelial sodium channel genes Scnn1b and Scnn1g are closely linked on distal mouse chromosome 7. Genomics 29: 784-786, 1995.
7. Canessa, C. M., J.-D. Horisberger, and B. C. Rossier. Epithelial sodium channel related to proteins involved in neurodegeneration. Nature 361: 467-470, 1993.
8. Canessa, C. M., A. M. Merillat, and B. C. Rossier. Membrane topology of the epithelial sodium channel in intact cells. Am. J. Physiol. 267 (Cell Physiol. 36): C1682-C1690, 1994.
9. + channel is made of three homologous subunits. Nature 367: 463-467, 1994.
10. Chang, S. S., S. Grunder, A. Hanukoglu, A. Rosier, P. M. Mathew, I. Hanukoglu, L. Schild, Y. Lu, R. A. Shimkets, C. N. Nelson-Williams, B. C. Rossier, and R. P. Lifton. Mutations in subunits of the epithelial sodium channel cause salt wasting with hyperkalemic acidosis, pseudohypoaldosteronism type 1. Nat. Genet. 12: 248-253, 1996.
11. Corey, D. P., and J. Garcia-Anoveros. Mechanosensation and the DEG/ENaC ion channels. Science 273: 323-324, 1996.
12. Dagenais, A., R. Kothary, and Y. Berthiaume. The alpha subunit of the epithelial sodium channel in the mouse: developmental regulation of its expression. Pediatr. Res. 42: 327-334, 1997.
13. Due, C., N. Farman, C. M. Canessa, J. P. Bonvalet, and B. C. Rossier. Cell-specific expression of epithelial sodium channel α, β, and γ subunits in aldosterone-responsive epithelia from the rat: localization by in situ hybridization and immunocytochemistry. J. Cell Biol. 127: 1907-1921, 1994.
14. Feinberg, A. P., and B. Vogelstein. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132: 6-13, 1983.
15. Firsov, D., I. Gautschi, A. M. Merillat, B. C. Rossier, and L. Schild. The heterotetrameric architecture of the epithelial sodium channel (ENaC). EMBO J. 17: 344-352, 1998.
16. + channel subunit. Am. J. Physiol. 269 (Cell Physiol. 38): C641-C654, 1995.
17. + channels. FASEB J. 8: 522-528, 1994.
18. + permeability by aldosterone. Semin. Nephrol. 12: 24-29, 1992.
19. Garty, H., and L. G. Palmer. Epithelial sodium channels: structure, function, and regulation. Physiol. Rev. 77: 359-396, 1997.
20. Geller, D. S., J. Rodriguez-Soriano, A. Vallo Boado, S. Schifter, M. Bayer, S. S. Chang, and R. P. Lifton. Mutations in the mineralocorticoid receptor gene cause autosomal dominant pseudohypoaldosteronism type I. Nat. Genet. 19: 279-281, 1998.
21. + channel subunits. Am. J. Physiol. 272 (Cell Physiol. 41): C270-C277, 1997.
22. Grunder, S., D. Firsov, S. S. Chang, N. F. Jaeger, I. Gautschi, L. Schild, R. P. Lifton, and B. C. Rossier. A mutation causing pseudohypoaldosteronism type-1 identifies a conserved glycine that is involved in the gating of the epithelial sodium-channel. EMBO J. 16: 899-907, 1997.
23. Hamilton, K. L., and D. C. Eaton. Single channel recordings from amiloride-sensitive epithelial sodium channel. Am. J. Physiol. 249 (Cell Physiol. 18): C200-C207, 1985.
24. Hansson, J. H., C. Nelson-Williams, H. Suzuki, L. Schild, R. Shimkets, Y. Lu, C. Canessa, T. Iwasaki, B. Rossier, and R. P. Lifton. Hypertension caused by a truncated epithelial sodium channel gamma-subunit: Genetic-heterogeneity of Liddle syndrome. Nat. Genet. 11: 76-82, 1995.
25. + channel. J. Biol. Chem. 271: 807-816, 1996.
26. Ji, H. L., C. M. Fuller, and D. J. Benos. Osmotic pressure regulates αβγ-rENaC expressed in Xenopus oocytes. Am. J. Physiol. 275 (Cell Physiol. 44): C1182-C1190, 1998.
27. Kizer, N., X. L. Guo, and K. Hruska. Reconstitution of stretch-activated cation channels by expression of the a-subunit of the epithelial sodium channel cloned from osteoblasts. Proc. Natl. Acad. Sci. USA 94: 1013-1018, 1997.
28. Kleyman, T. R., and E. J. Cragoe, Jr. Cation transport probes: the amiloride series. In: Methods in Enzymology. Cellular and Subcellular Transport: Epithelial Cell, edited by S. Fleischer and B. Fleischer. Orlando: Academic, 1990, p. 739-755.
29. Kosari, F., S. Sheng, J. Li, D. D. Mak, J. K. Foskett, and T. R. Kleyman. Subunit stoichiometry of the epithelial sodium channel. J. Biol. Chem. 273: 13469-13474, 1998.
30. + channel. FEBS Lett. 318: 95-99, 1993.
31. McDonald, F. J., M. P. Price, P. M. Snyder, and M. J. Welsh. Cloning and expression of the β- and γ-subunits of the human epithelial sodium channel. Am. J. Physiol. 268 (Cell Physiol. 37): C1157-C1163, 1995.
32. + channel. Am. J. Physiol. 266 (Lung Cell. Mol. Physiol. 10): L728-L734, 1994.
33. Nelson, W. J. Regulation of cell surface polarity from bacteria to mammals. Science 258: 948-955, 1992.
34. Palmer, L. G., and G. Frindt. Gating of Na channels in the rat cortical collecting tubule: effects of voltage and membrane stretch. J. Gen. Physiol. 107: 35-45, 1996.
35. Palmer, L. G. Epithelial Na channels: function and diversity. Annu. Rev. Physiol. 54: 51-66, 1992.
36. Puoti, A., A. May, C. M. Canessa, J. D. Horisberger, L. Schild, and B. C. Rossier. The highly selective low-conductance epithelial Na channel of Xenopus laevis A6 kidney cells. Am. J. Physiol. 269 (Cell Physiol. 38): C188-C197, 1995.
37. + channel. J. Biol. Chem. 269: 12981-12986, 1994.
38. Rodriguez-Boulan, E., and W. J. Nelson. Morphogenesis of the polarized epithelial cell phenotype. Science 245: 718-725, 1989.
39. Rossier, B. C., C. M. Canessa, L. Schild, and J. D. Horisberger. Epithelial sodium channels. Curr. Opin. Nephrol. Hypertens. 3: 487-496, 1994.
40. + channel (alpha-rENaC) mediates its localization at the apical membrane. EMBO J. 13: 4440-4450, 1994.
41. Sambrook, J., E. F. Fritsch, and T. Maniatis. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989.
42. Sanger, F. G., S. Nicklen, and A. R. Coulson. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463-5467, 1977.
43. Schild, L., X. Lu, I. Gautschi, E. Schneeberger, R. P. Lifton, and B. C. Rossier. Identification of a PY motif in the epithelial Na channel subunits as a target sequence for mutations causing channel activation found in Liddle syndrome. EMBO J. 15: 2381-2387, 1996.
44. Schild, L., E. Schneeberger, I. Gautschi, and D. Firsov. Identification of amino acid residues in the α, β, and γ subunits of the epithelial sodium channel (ENaC) involved in amiloride block and ion permeation. J. Gen. Physiol. 109: 15-26, 1997.
45. Sheng, S., J. Li, and T. R. Kleyman. Epithelial sodium channel cation selectivity is altered by a mutation of a tryptophan residue (Abstract). J. Am. Soc. Nephrol. 9: 45, 1998.
46. Shimkets, R., R. Lifton, and C. Canessa. The activity of the epithelial sodium channel is regulated by clathrin-mediated endocytosis. J. Biol. Chem. 272: 25537-25541, 1997.
47. Shimkets, R., R. Lifton, and C. Canessa. In vivo phosphorylation of the epithelial sodium channel. Proc. Natl. Acad. Sci. USA 95: 3301-3305, 1998.
48. Shimkets, R. A., D. G. Warnock, C. M. Bositis, C. Nelson-Williams, J. H. Hansson, M. Schambelan, J. R. Gill, Jr., S. Ulick, R. V. Milora, J. W. Findling, C. M. Canessa, B. C. Rossier, and R. P. Lifton. Liddle's syndrome: heritable human hypertension caused by mutations in the β subunit of the epithelial Na channel. Cell 79: 407-414, 1994.
49. + channels. Annu. Rev. Physiol. 53: 509-530, 1991.
50. Smith, P. R., S. A. Mackler, P. C. Weiser, D. R. Brooker, Y. J. Ahn, B. J. Harte, K. A. McNulty, and T. R. Kleyman. Expression and localization of epithelial sodium channel in mammalian urinary bladder. Am. J. Physiol. 274 (Renal Physiol. 43): F91-F96, 1998.
51. Snyder, P. M., C. Cheng, L. S. Prince, J. C. Rogers, and J. M. Welsh. Electrophysiological and biochemical evidence that DEG/ ENaC cation channels are composed of nine subunits. J. Biol. Chem. 273: 681-684, 1998.
52. Snyder, P. M., F. J. McDonald, J. B. Stokes, and M. J. Welsh. Membrane topology of the amiloride-sensitive epithelial sodium channel. J. Biol. Chem. 269: 24379-24383, 1994.
53. Snyder, P. M., M. P. Price, F. J. McDonald, C. M. Adams, K. A. Volk, B. G. Zeiher, J. B. Stokes, and M. J. Welsh. Mechanism by which Liddle's syndrome mutations increase activity of a human epithelial Na channel. Cell 83: 969-978, 1995.
54. + channel deleted in Liddle's syndrome. EMBO J. 15: 2371-2380, 1996.
55. Thomas, C. P., S. D. Auerbach, C. Zhang, and J. B. Stokes. The structure of the rat amiloride-sensitive epithelial sodium channel gamma subunit gene and functional analysis of its promoter. Gene 228: 111-122, 1999.
56. + channels contain multiple intersubunit association sites. J. Biol. Chem. 271: 18904-18911, 1996.
57. Tymms, M. In vitro transcription and translation protocols. In: Methods in Molecular Biology. Totowa, NJ: Humana, 1995.
58. Verrey, F. Transcriptional control of sodium transport in tight epithelia by adrenal steroids. J. Membr. Biol. 143: 93-110, 1995.
59. + channel: biophysical properties, pharmacology, ontogenesis, and molecular cloning. Proc. Natl. Acad. Sci. USA 91: 247-251, 1994.
60. + channel function. J. Biol. Chem. 270: 11735-11737, 1995.
61. + conductance of rat hepatocytes in primary culture. J. Gen. Physiol. 105: 507-535, 1995.