Inhibition of apolipoprotein B and triglyceride secretion in human hepatoma cells (HepG2)

Journal of Lipid Research

Volumn 37, Issue 7, 1996, Pages 1468-1480

  Haghpassand, Mehrdad ^a   Wilder, Don ^a   Moberly, James B ^a,b  

^a PFIZER INC   (United States)

^b BAXTER HEALTHCARE CORPORATION   (United States)

Author keywords

Caco 2 cells; enterocyte; hepatocyte; methaqualone; microsomal triglyceride transfer protein (MTP); triacylglycerol; very low density lipoprotein assembly

Indexed keywords

APOLIPOPROTEIN B; LIPOSOME; METHAQUALONE; TRIACYLGLYCEROL; VERY LOW DENSITY LIPOPROTEIN;

ARTICLE; ENDOPLASMIC RETICULUM; HEPATOMA CELL; HUMAN; HUMAN CELL; HYBRIDIZATION; HYPERLIPIDEMIA; INTESTINE CELL; LIPOPROTEIN SYNTHESIS; LIVER MICROSOME; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN CONFORMATION; PROTEIN FOLDING;

APOLIPOPROTEINS B; CACO-2 CELLS; CARCINOMA, HEPATOCELLULAR; CARRIER PROTEINS; CHOLESTEROL ESTER TRANSFER PROTEINS; GLYCEROL; GLYCOPROTEINS; HUMANS; LIPOSOMES; METHAQUALONE; OLEIC ACID; RNA, MESSENGER; TRIGLYCERIDES; TRITIUM; TUMOR CELLS, CULTURED;

EID: 0029996677     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (49)

1. Boström, K., M. Wettesten, J. Borén, G. Bondjers, O. Wiklund, and S-O. Olofsson. 1986. Pulse-chase studies of the synthesis and intracellular transport of apolipoprotein B-100 in HepG2 cells. J. Biol. Chem. 261: 13800-13806.
2. Davis, R. A., R. N. Thrift, C. C. Wu, and K. E. Howell. 1990. Apolipoprotein B is both integrated into and translocated across the endoplasmic reticulum membrane. J. Biol. Chem. 265: 10005-10011.
3. Borén, J., L. Graham, M. Wettesten, J. Scott, A. White, and S-O. Olofsson. 1992. The assembly and secretion of apoB-100-containing lipoproteins in HepG2 cells. J. Biol. Chem. 267:9858-9867.
4. Bamberger, M. J., and M. D. Lane. 1988. Assembly of very low density lipoproteins in the hepatocyte. Differential transport of apoproteins through the secretory pathway. J. Biol. Chem. 263: 11868-11878.
5. Dixon, J. L., R. Chattapadhyay, T. Huima, C. M. Redman, and D. Banerjee. 1992. Biosynthesis of lipoprotein: location of nascent apoA-I and apoB in the rough endoplasmic reticulum of chicken hepatocytes. J. Cell. Biol. 117: 1161-1169.
6. Bamberger, M. J., and M. D. Lane. 1990. Possible role of the Golgi apparatus in the assembly of very low density lipoprotein. Proc. Natl. Acad. Sci. USA. 87: 2390-2394.
7. Hamilton, R. L., A. Moorehouse, and R. J. Havel. 1991. Isolation and properties of nascent lipoproteins from highly purified rat hepatocytic Golgi fractions. J. Lipid Res. 32: 529-543.
8. Borén, J., M. Wetteston, A. Sjöberg, T. R. Thorlin, G. Bondjers, O. Wiklund, and S-O. Olofsson. 1990. The assembly and secretion of apoB-100-containing lipoproteins in HepG2 cells. J. Biol. Chem. 265: 10556-10564.
9. Boström, K., J. Borén, M. Wettesten, A. Sjöberg, G. Bondjers, O. Wiklund, P. Carlsson, and S-O. Olofsson. 1988. Studies on the assembly of apoB-100-containing lipoproteins in HepG2 cells. J. Biol. Chem. 263: 4434-4442.
10. Pullinger, C. R., J. D. North, B-B. Teng, V. A. Rifici, A. E. Ronhild de Brito, and J. Scott. 1989. The apolipoprotein B gene is constitutively expressed in HepG2 cells: regulation of secretion by oleic acid, albumin, insulin and measurement of the mRNA half-life. J. Lipid Res. 30: 1065-1077.
11. Moberly, J. B., T. G. Cole, D. H. Alpers, and G. Schonfeld. 1990. Oleic acid stimulation of apolipoprotein B secretion from HepG2 and Caco-2 cells occurs post-transcriptionally. Biochim. Biophys. Acta. 1042: 70-80.
12. Sato, R., T. Imanaka, A. Takatsuki, and T. Takano. 1990. Degradation of newly synthesized apolipoprotein B-100 in a pre-Golgi compartment. J. Biol. Chem. 265: 11880-11884.
13. Furukawa, S., N. Sakata, H. N. Ginsberg, and J. L. Dixon. 1992. Studies of the sites of intracellular degradation of apolipoprotein B in HepG2 cells. J. Biol. Chem. 267: 22630-22638.
14. Dixon, J. L., S. Furukawa, and H. N. Ginsberg. 1991. Oleate stimulates secretion of apolipoprotein B-containing lipoproteins from HepG2 cells by inhibiting early intracellular degradation of apolipoprotein B. J. Biol. Chem. 266: 5080-5086.
15. White, A. L., D. L. Graham, J. LeGros, R. J. Pease, and J. Scott. 1992. Oleate-mediated stimulation of apolipoprotein B secretion from rat hepatoma cells. J. Biol. Chem. 267: 15657-15664.
16. Wang, H., X. Chen, and E. A. Fisher. 1993. N-3 fatty acids stimulate intracellular degradation of apoprotein B in rat hepatocytes. J. Clin. Invest. 91: 1380-1389.
17. Wetterau, J. R., L. P. Aggerbeck, M-E. Bouma, C. Eisenberg, A. Munck, M. Hermier, J. Schmitz, G. Gay, D. J. Rader, and R. E. Gregg. 1992. Absence of microsomal triglyceride transfer protein in individuals with abetalipoproteinemia. Science. 258: 999-1001.
18. Wetterau, J. R., and D. B. Zilversmit. 1984. A triglyceride and cholesteryl ester transfer protein associated with liver microsomes. J. Biol. Chem. 259: 10863-10866.
19. Lackner, K. J., J. C. Monge, R. E. Gregg, J. M. Hoeg, T. J. Triche, S. W. Law, and H. B. Brewer, Jr. 1986. Analysis of the apolipoprotein B gene and messenger ribonucleic acid in abetalipoproteinemia. J. Clin. Invest. 78: 1707-1712.
20. Sharp, D., L. Blinderman, K. A. Combs, B. Kienzle, B. Ricci, K. Wager-Smith, C. M. Gil, C. W. Turck, M-E. Bouma, D. J. Rader, L. P. Aggerbeck, R. E. Gregg, D. A. Gordon, and J. R. Wetterau. 1993. Cloning and gene defects in microsomal triglyceride transfer protein associated with abetalipoproteinaemia. Nature. 365: 65-69.
21. Shoulders, C. C., D. J. Brett, J. D. Bayliss, T. M. E. Narcisi, A. Jarmuz, T. T. Grantham, P. R. D. Leoni, S. Bhattacharya, R.J. Pease, P. M. Cullen, S. Levi, P. G. H. Byfield, P. Purkiss, and J. Scott. 1993. Abetalipoproteinemia is caused by defects of the gene encoding the 97 kDa subunit of a microsomal triglyceride transfer protein. Human Mol. Genet. 2: 2109-2116.
22. Leiper, J. M., J. D. Bayliss, J. P. Richard, D. J. Brett, J. Scott, and C. C. Shoulders. 1994. Microsomal triglyceride transfer protein, the abetalipoproteinemia gene product, mediates the secretion of apolipoprotein B-containing lipoproteins from heterologous cells. J. Biol. Chem. 269: 21951-21954.
23. Gordon, D. A., H. Jamil, D. Sharp, D. Mullaney, Z. Yao, R. E. Gregg, and J. Wetterau. 1994. Secretion of apolipoprotein B-containing lipoproteins from HeLa cells is dependent on expression of the microsomal triglyceride transfer protein and is regulated by lipid availability. Proc. Natl. Acad. Sci. USA. 91: 7628-7632.
24. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
25. Smith, P. K., R. I. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, N. M. Goeke, B. J. Olson, and D. C. Klenk. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150: 76-85.
26. Haghpassand, M., and J. B. Moberly. 1995. 9-cis-Retinoic acid increases apolipoprotein A-I secretion and mRNA expression in HepG2 cells. Atherosclerosis. 117: 199-207.
27. Cole, T. G., R. Kitchens, A. Daugherty, and G. Schonfeld. 1988. An improved method for separation of triglyceride-rich lipoproteins by FPLC. Pharmacia FPLC Biocommunique. 4: 4.
28. Wetterau, J. R., and D. B. Zilversmit. 1986. Localization of intracellular triacylglycerol and cholesteryl ester transfer activity. Biochim. Biophys. Acta. 875: 610-617.
29. Clark, R. W., J. B. Moberly, and M. J. Bamberger. 1995. Low level quantification of cholesteryl ester transfer protein in plasma subfractions and cell culture media by monoclonal antibody-based immunoassay. J. Lipid Res. 36:876-889.
30. Jiao, S., J. B. Moberly, and G. Schonfeld. 1990. Editing of apolipoprotein B messenger RNA in differentiated Caco-2 cells. J. Lipid Res. 31: 695-700.
31. Thrift, R. N., T. M. Forte, B. E. Cahoon, and V. G. Shore. 1986. Characterization of lipoproteins produced by the human liver cell line, HepG2, under defined conditions. J. Lipid Res. 27: 236-250.
32. Dixon, J. L., and H. Ginsberg. 1993. Regulation of hepatic secretion of apolipoprotein B-containing lipoproteins: information obtained from cultured liver cells. J. Lipid Res. 34: 167-179.
33. Powell, L. M., S. C. Wallis, R. J. Pease, Y. H. Edwards, T. J. Knott, and J. Scott. 1987. A novel form of tissue-specific RNA processing produces apolipoprotein B-48 in intestine. Cell. 50: 831-840.
34. Sakata, N., X. Wu, J. L. Dixon, and H. N. Ginsberg. 1993. Proteolysis and lipid-facilitated translocation are distinct but competitive processes that regulate secretion of apolipoprotein B in HepG2 cells. J. Biol. Chem. 268: 22967-22970.
35. Chuck, S. L., Z. Yao, B. D. Blackhart, B. J. McCarthy, and V. R. Lingappa. 1990. New variation on the translocation of proteins during early biogenesis of apolipoprotein B. Nature. 346: 382-385.
36. Cianflone, K. M., Z. Yasruel, M. A. Rodriguez, D. Vas, and A. D. Sniderman. 1990. Regulation of apoB secretion from HepG2 cells: evidence for a critical role for cholesteryl ester synthesis in the response to a fatty acid challenge. J. Lipid Res. 31: 2045-2055.
37. Wu, X., N. Sakata, E. Lui, and H. N. Ginsberg. 1994. Evidence for a lack of regulation of the assembly and secretion of apolipoprotein B-containing lipoprotein from HepG2 cells by cholesteryl ester. J. Biol. Chem. 269: 12375-12382.
38. Lang, C. A., and R. A. Davis. 1990. Fish oil fatty acids impair VLDL assembly and/or secretion by cultured rat hepatocytes. J. Lipid Res. 31: 2079-2086.
39. Sparks, J. D., and C. E. Sparks. 1990. Insulin modulation of hepatic synthesis and secretion of apolipoprotein B by rat hepatocytes. J. Biol. Chem. 265: 8854-8862.
40. Rusiñol, A. E., E. Y. W. Chan, and J. E. Vance. 1993. Movement of apolipoprotein B into the lumen of microsomes from hepatocytes is disrupted in membranes enriched in phosphatidylmonomethylethanolamine. J. Biol. Chem. 268: 25168-25175.
41. Arbeeny, C. M., D. S. Meyers, K. E. Bergquist, and R. E. Gregg. 1992. Inhibition of fatty acid synthesis decreases very low density lipoprotein secretion in the hamster. J. Lipid Res. 33: 843-851.
42. Yao, Z., B. D. Blackart, M. F. Linton, S. M. Taylor, S. G. Young, and B. J. McCarthy. 1991. Expression of carboxyl-terminally truncated forms of human apolipoprotein B in rat hepatoma cells. J. Biol. Chem. 266: 3300-3308.
43. Thrift, R. N., J. Drisko, S. Dueland, J. D. Trawick, and R. A. Davis. 1992. Translocation of apolipoprotein B across the endoplasmic reticulum is blocked in a non-hepatic cell line. Proc. Natl. Acad. Sci. USA. 89: 9161-9165.
44. Rusiñol, A. E., E. Y. W. Chan, and J. E. Vance. 1993. Assembly of rat hepatic very low density lipoproteins in the endoplasmic reticulum. J. Biol. Chem. 268: 3555-3562.
45. Lin, M. C. M., C. Arbeeny, K. Bergquist, B. Kienzle, D. A. Gordon, and J. R. Wetterau. 1994. Cloning and regulation of hamster microsomal triglyceride transfer protein. J. Biol. Chem. 269: 29138-29145.
46. Teng, B., A. D. Sniderman, A. K. Soutar, and G. R. Thompson. 1986. Metabolic basis of hyperapobetalipoproteinemia: turnover of apolipoprotein B in low density lipoprotein and its precursors and subfractions compared with normal and familial hypercholesterolemia. J. Clin. Invest. 77: 663-672.
47. Venkatesan, S., P. Cullen, P. Pacy, D. Halliday, and J. Scott. 1993. Stable isotopes show a direct relation between VLDL apoB overproduction and serum triglyceride levels and indicate a metabolically and biochemically coherent basis for familial combined hyperlipidemia. Arterioscler. Thromb. 13: 1110-1118.
48. Grundy, S. M., A. Chait, and J. D. Brunzell. 1987. Familial combined hyperlipidemia workshop. Arteriosclerosis. 7: 203-207.
49. Sniderman, A. D., S. Shapiro, D. Marpole, B. Skinner, B. Teng, and P. O. Kwiterovich, Jr. 1980. Association of coronary atherosclerosis with hyperapobetalipoproteinemia (increased protein but normal cholesterol levels in human plasma low density lipoproteins). Proc. Natl. Acad. Sci. USA. 77: 604-608.