Role of lipid synthesis, chaperone proteins and proteasomes in the assembly and secretion of apoprotein B-containing lipoproteins from cultured liver cells

Clinical and Experimental Pharmacology and Physiology

Volumn 24, Issue 5, 1997, Pages a29-a32

  Ginsberg, Henry N ^a  

^a Coll Phys Surgs of Columbia Univ ^*  (United States)

Author keywords

apolipoprotein B; degradation; heat shock protein 70; hepatocytes

Indexed keywords

APOLIPOPROTEIN B; CHAPERONE; CHYLOMICRON; LOW DENSITY LIPOPROTEIN; VERY LOW DENSITY LIPOPROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; DYSLIPIDEMIA; ENDOPLASMIC RETICULUM; GENETIC VARIABILITY; HUMAN; HUMAN TISSUE; LIGAND BINDING; LIPOGENESIS; LIVER CELL CARCINOMA; LIVER CELL CULTURE; POPULATION RESEARCH; PROTEIN ASSEMBLY; PROTEIN DEGRADATION; PROTEIN PROCESSING; PROTEIN SECRETION; PROTEIN SYNTHESIS REGULATION;

EID: 0030906716     PISSN: 03051870     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1440-1681.1997.tb03051.x     Document Type: Article

References (61)

1. Sturley, S. L., P. A. Talmud, R. Brasseur, M. R. Culbertson, S. E. Humphries and A. D. Attie. 1994. Human apolipoprotein B signal sequence variants confer a secretion-defective phenotype when expressed in yeast. J. Biol. Chem. 34: 21670-5 .
2. Pullinger, C. R., J. D. North, B. Teng, V. A. Rifici, A. E. Ronhild de Brito and J. Scott. 1989. The apolipoprotein B gene is constitutively expressed in HepG2 cells: Regulation of secretion by oleic acid, albumin, and insulin, and measurement of the mRNA half-life. J. Lipid Res. 30: 1065-76.
3. Moberly, J. B., T. G. Cole, D. H. Alpers and G. Schonfeld. 1990. Oleic acid stimulation of apolipoprotein B secretion from HepG2 and Caco-2 cells occurs post-transcriptionally. Biochim. Biophys. Acta 1042: 70-80.
4. Dashti, N., D. L. Williams and P. Alaupovic. 1989. Effects of oleate and insulin on the production rates and cellular mRNA concentrations of apolipoproteins in HepG2 cells. J. Lipid Res. 30: 1365-73.
5. Wu, X., N. Sakata, J. L. Dixon and H. N. Ginsberg. 1994. Exogenous VLDL stimulates apolipoprotein from HepG2 cells by both pre- and post-translational mechanisms. J. Lipid Res. 35:1200-10.
6. Dashti, N. 1992. The effect of low density lipoproteins, cholesterol, and 25-hydroxycholesterol on apolipoprotein B gene expression in HepG2 cells. J. Biol. Chem. 267: 7160-9.
7. Palade, G. 1975. Intracellular aspects of the process of protein synthesis. Science 189: 347-58.
8. Gilmore, R. and G. Blobel. 1985. Translocation of secretory proteins across the microsomal membrane occurs through an environment accessible to aqueous perturbants. Cell 42: 497-505.
9. Ng, D. T. W. and P. Walter. 1994. Protein translocation across the endoplasmic reticulum. Curr. Opin. Cell Biol. 6: 510-16.
10. Bostrom, K., M. Wettesten, J. Borén, G. Bondjers, O. Wiklund and S. Olofsson. 1986. Pulse-chase studies of the synthesis and intracellular transport of apolipoprotein B-100 in HepG2 cells. J. Biol. Chem. 261: 13800-6.
11. Bostrom, K., J. Borén, M. Wettesten et al. 1988. Studies on the assembly of Apo B-100-containing lipoproteins in HepG2 cells. J. Biol. Chem. 263: 4434-42.
12. Borén, J., M. Wettesten, A. Sjöberg et al. 1990. The assembly and secretion of ApoB 100 containing lipoproteins in Hep G2 cells. Evidence for different sites for protein synthesis and lipoprotein assembly. J. Biol. Chem. 265: 10556-64.
13. Bamberger, M. J. and M. D. Lane. 1988. Assembly of very low density lipoprotein in the hepatocyte. Differential transport of apoproteins through the secretory pathway. J. Biol. Chem. 263: 11868-78.
14. Davis, R. A., A. B. Prewett, D. C. F. Chan, J. J. Thompson, R. A. Borchardt and W. R. Gallaher. 1989. Intrahepatic assembly of very low density lipoproteins: Immunologic characterization of apolipoprotein B in lipoproteins and hepatic membrane fractions and its intracellular distribution. J. Lipid Res. 30: 1185-96.
15. Dixon, J. L., T. Huima and D. Banerjee. 1989. Location of nascent apoproteins A-I and B in the rough endoplasmic reticulum. J. Cell Biol. 109: 148a.
16. Dixon, J. L., R. Chattapadhyay, T. Huima, C. M. Redman and D. Banerjee. 1992. Biosynthesis of lipoprotein: Location of nascent ApoAI and ApoB in the rough endoplasmic reticulum of chicken hepatocytes. J. Cell Biol. 117: 1161-9.
17. Wilkinson, J., J. A. Higgins, P. Groot, E. Gherardi and D. Bowyer. 1993. Topography of apolipoprotein B in subcellular fractions of rabbit liver probed with a panel of monoclonal antibodies. J. Lipid Res. 34: 815-25.
18. Wilkinson, J., J. A. Higgins, P. H. E. Groot, E. Gherardi and D. E. Bowyer. 1992. Determination of the intracellular distribution and pool sizes of apolipoprotein B in rabbit liver. Biochem. J. 288: 413-19.
19. Furukawa, S., N. Sakata, H. N. Ginsberg and J. L. Dixon. 1992. Studies of the sites of intracellular degradation of apolipoprotein B in Hep G2 cells. J. Biol. Chem. 267: 22630-8.
20. Du, E. Z., J. Kurth, S. Wang, P. Humiston and R. A. Davis. 1994. Proteolysis-coupled secretion of the N terminus of apolipoprotein B: Characterization of a transient, translocation arrested intermediate. J. Biol. Chem. 39: 24169-76.
21. McLeod, R. S., Y. Wang, S. Wang, A. Rusinol, P. Links and Z. Yao. 1996. Apolipoprotein B sequence requirements for hepatic very low density lipoprotein assembly. Evidence that hydrophobic sequences within apolipoprotein B48 mediate lipid recruitment. J. Biol. Chem. 271: 18445-55.
22. Wang, S., R. S. McLeod, D. A. Gordon and Z. Yao. 1996. The microsomal triglyceride transfer protein facilitates assembly and secretion of apolipoprotein B-containing lipoproteins and decreases cotranslational degradation of apolipoprotein B in transfected COS-7 cells. J. Biol. Chem. 271: 14124-33.
23. Pease, R. J., G. B. Harrison and J. Scott. 1991. Cotranslational insertion of apolipoprotein B into the inner leaflet of the endoplasmic reticulum. Nature 353: 448-50.
24. Ingram, M. F. and G. S. Shelness. 1996. Apolipoprotein B-100 destined for lipoprotein assembly and intracellular degradation undergoes efficient translocation across the endoplasmic reticulum membrane. J. Lipid Res. 37: 2202-14.
25. Leiper, J. M., G. B. Harrison, J. D. Bayliss, J. Scott and R. J. Pease. 1996. Systematic expression of the complete coding sequence of apoB-100 does not reveal transmembrane determinants. J. Lipid Res. 37: 2215-31.
26. Chuck, S. L. and V. R. Lingappa. 1992. Pause transfer: A topogenic sequence in apolipoprotein B mediates stopping and restarting of translocation. Cell 68: 9-21.
27. Nakahara, D. H., V. R. Lingappa and S. L. Chuck. 1994. Translocational pausing is a common step in the biogenesis of unconventional integral membrane and secretory proteins. J. Biol. Chem. 269: 7617-22.
28. Yang, C., Z. Gu, S. Weng et al. 1989. Structure of apolipoprotein B-100 of human low density lipoproteins. Arteriosclerosis 9: 96-108.
29. Segrest, J. P., M. K. Jones, V. K. Mishra, G. M. Anantharamaiah and D. W. Garber. 1994. ApoB-100 has a pentapartite structure composed of three amphipathic alpha-helical domains alternating with two amphipathic beta-strand domains. Detection by the computer program LOCATE. Arterioscler. Thromb. 14: 1674-85.
30. Shelness, G. S., K. C. Morris-Rogers and M. F. Ingram. 1994. Apolipoprotein B48-membrane interactions: Absence of transmembrane localization in nonhepatic cells. J. Biol. Chem. 269: 9310-18.
31. Borchardt, R. A. and R. A. Davis. 1987. Intrahepatic assembly of very low density lipoproteins. Rate of transport out of the endoplasmic reticulum determines rate of secretion. J. Biol. Chem. 262: 16394-402.
32. Dixon, J. L., S. Furukawa and H. N. Ginsberg. 1991. Oleate stimulates secretion of apolipoprotein B-containing lipoproteins from Hep G2 cells by inhibiting early intracellular degradation of apolipoprotein B. J. Biol. Chem. 266: 5080-6.
33. Sparks, J. D. and C. E. Sparks. 1990. Insulin modulation of hepatic synthesis and secretion of apolipoprotein B by rat hepatocytes. J. Biol. Chem. 265: 8854-62.
34. Sparks, J. D. and C. E. Sparks. 1994. Insulin regulation of triacylglycerol-rich lipoprotein synthesis and secretion. Biochim. Biophys. Acta 1215: 9-32.
35. Sato, R., T. Imanaka, A. Takatsuki and T. Takano. 1990. Degradation of newly synthesized apolipoprotein B-100 in a pre-Golgi compartment. J. Biol. Chem. 265: 11880-4.
36. Thrift, R. N., J. Drisko, S. Dueland, J. D. Trawick and R. A. Davis. 1992. Translation of apolipoprotein B across the endoplasmic reticulum is blocked in a nonhepatic cell line. Proc. Natl Acad. Sci. USA 89: 9161-5.
37. Adeli, K. 1994. Regulated intracellular degradation of apolipoprotein B in semipermeable HepG2 cells. J. Biol. Chem. 269: 9166-75.
38. Sallach, S. M. and K. Adeli. 1995. Intracellular degradation of apolipoprotein B generates an N-terminal 70 kDa fragment in the endoplasmic reticulum. Biochim. Biophys. Acta 1265: 29-32.
39. Dixon, J. L. and H. N. Ginsberg. 1993. Regulation of hepatic secretion of apolipoprotein B-containing lipoproteins: Information obtained from cultured liver cells. J. Lipid Res. 34: 167-79.
40. Wu, X., N. Sakata, E. Lui and H. N. Ginsberg. 1994. Evidence for a lack of regulation of the assembly and secretion of apolipoprotein B-containing lipoprotein from HepG2 cells by cholesteryl ester. J. Biol. Chem. 269: 12375-82.
41. Cianflone, K. M., Z. Yasruel, M. A. Rodriguez, D. Vas and A. D. Sniderman. 1990. Regulation of apoB secretion from HepG2 cells: Evidence for a critical role for cholesteryl ester synthesis in the response to a fatty acid challenge. J. Lipid Res. 31: 2045-55.
42. Arad, Y., R. Ramakrishnan and H. N. Ginsberg. 1990. Lovastatin therapy reduces low density lipoprotein apoB levels in subjects with combined hyperlipidemia by reducing the production of apoB-containing lipoproteins: Implications for the pathophysiology of apoB production. J. Lipid Res. 31: 567-82.
43. Khan, B., H.G. Wilcox and M. Heimberg. 1989. Cholesterol is required for secretion of very low density lipoprotein by rat liver. Biochem. J. 258: 807-16.
44. Fungwe, T. V., L. Cagen, H. G. Wilcox and M. Heimberg. 1992. Regulation of hepatic secretion of very low density lipoprotein by dietary cholesterol. J. Lipid Res. 33: 179-91.
45. Fuki, I. V., S. N. Preobrazhensky, A. Y. Mishavin et al. 1989. Effect of cell cholesterol content on apolipoprotein B secretion and LDL receptor activity in the human hepatoma cell line, Hep G2. Biochim. Biophys. Acta 1001: 235-8.
46. Vance, J. E. and D. E. Vance. 1990. Lipoprotein assembly and secretion by hepatocytes. Annu. Rev. Nutr. 10: 337-56.
47. Yao, Z. and D. E. Vance. 1988. The active synthesis of phosphatidylcholine is required for very low density lipoprotein secretion from rat hepatocytes. J. Biol. Chem. 263: 2998-3004.
48. Rusinol, A. E., E. Y. W. Chan and J. E. Vance. 1994. Movement of apolipoprotein B into the lumen of microsomes from hepatocytes is disrupted in membranes enriched in phosphatidylmonomethylethanolamine. J. Biol. Chem. 268: 25168-75.
49. Sharp, D., L. Blinderman, K. A. Combs et al. 1993. Cloning and gene defects in microsomal triglyceride transfer protein associated with abetalipoproteinaemia. Nature 365: 65-9.
50. Shoulders, C. C., D. J. Brett, J. D. Bayliss et al. 1993. Abetalipoproteinemia is caused by defects of the gene encoding the 97 kDa sub-unit of a microsomal triglyceride transfer protein. Hum. Mol. Genet. 2: 2109-16.
51. Gordon, D. A., H. Jamil, D. Sharp et al. 1994. Secretion of apolipoprotein B-containing lipoproteins from HeLa cells is dependent on expression of the microsomal triglyceride transfer protein and is regulated by lipid availability. Proc. Natl Acad. Sci. USA 91: 7628-32.
52. Leiper, J. M., J. D. Bayliss, R. J. Pease, D. J. Brett, J. Scott and C. C. Shoulders. 1994. Microsomal triglyceride transfer protein, the abetalipoproteinemia gene product, mediates the secretion of apolipoprotein B-containing lipoproteins from heterologous cells. J. Biol. Chem. 269: 21951-4.
53. Wu, X., M. Zhou, L. Huang, J. Wetterau and H. N. Ginsberg. 1996. Demonstration of a physical interaction between microsomal triglyceride transfer protein and apolipoprotein B during the assembly of apo B-containing lipoproteins. J. Biol. Chem. 271: 10277-81.
54. Wang, H., Z. Yao and E. Fisher. 1994. The effect of n-3 fatty acids on the secretion of carboxyl-terminally truncated forms of human apoprotein B. J. Biol. Chem. 269: 18514-20.
55. Yao, Z., B. D. Blackhart, M. F. Linton, S. M. Taylor, S. G. Young and B. J. McCarthy. 1991. Expression of carboxyl-terminally truncated forms of human apolipoprotein B in rat hepatoma cells. J. Biol. Chem. 5: 3300-8.
56. White, A. L., D. L. Graham, J. LeGros, R. J. Pease and J. Scott. 1992. Oleate-mediated stimulation of apolipoprotein B secretion from rat hepatoma cells. J. Biol. Chem. 267: 15657-64.
57. Zhou, M., X. Wu, L. Huang and H. Ginsberg. 1995. Apolipoprotein B100, an inefficiently translocated secretory protein, is bound to a cytosolic chaperone, heat shock protein 70. J. Biol. Chem. 270: 25220-4.
58. Ou, W., P. H. Cameron, D. Y. Thomas and J. J. M. Bergeron. 1993. Association of folding intermediates of glycoproteins with calnexin during protein maturation. Nature 364: 771-6.
59. Bergeron, J. J. M., M. B. Brenner, D. Y. Thomas and D. B. Williams. 1994. Calnexin: A membrane-bound chaperone of the endoplasmic reticulum. Trends Biochem. Sci. 19: 124-8.
60. Sakata, N., X. Wu, J. L. Dixon and H. N. Ginsberg. 1993. Proteolysis and lipid facilitated translocation are distinct but competitive processes which regulate secretion of apolipoprotein B in HepG2 cells. J. Biol. Chem. 268: 22967-70.
61. Yeung, S. J., S. H. Chen and L. Chan. 1996. Ubiquitin-proteasome pathway mediates intracellular degradation of apolipoprotein B. Biochemistry 35: 13843-8.