Assembly and secretion of VLDL in nondifferentiated Caco-2 cells stably transfected with human recombinant ApoB48 cDNA

Arteriosclerosis, Thrombosis, and Vascular Biology

Volumn 17, Issue 11, 1997, Pages 2955-2963

  Luchoomun, Jayraz ^a   Zhou, Zhangyin ^a   Bakillah, Ahmed ^a   Jamil, Haris ^b   Mahmood Hussain, M ^a  

^a DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

^b BRISTOL MYERS SQUIBB   (United States)

Author keywords

Apolipoprotein B; Chylomicrons; Intestine; Microsomal triglyceride transfer protein; Triglycerides

Indexed keywords

APOLIPOPROTEIN B; CHOLESTEROL ESTER; CHYLOMICRON; OLEIC ACID; TRIACYLGLYCEROL; VERY LOW DENSITY LIPOPROTEIN;

ARTICLE; CELL DIFFERENTIATION; CULTURE MEDIUM; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INTESTINE CELL; LIPID METABOLISM; LIPOGENESIS; MICROSOME; PRIORITY JOURNAL;

EID: 0031471692     PISSN: 10795642     EISSN: None     Source Type: Journal    
DOI: 10.1161/01.ATV.17.11.2955     Document Type: Article

References (19)

1. Madara JL, Trier JS. The functional morphology of the mucosa of the small intestine. In: Johnson LR, ed. Physiology of the Gastrointestinal Tract. New York, NY: Raven Press; 1994:1577-1622.
2. Hussain MM, Kancha RK, Zhou Z, Luchoomun J, Zu H, Bakillah A. Chylomicron assembly and catabolism: role of apolipoproteins and receptors. Biochim Biophys Acta. 1996;1300:151-170.
3. Levy E, Mehran M, Seidman E. Caco-2 cells as a model for intestinal lipoprotein synthesis and secretion. FASEB J. 1995;9:626-635.
4. Tso P, Balint JA. Formation and transport of chylomicrons by enterocytes to the lymphatics. Am J Physiol. 1986;250:G715-G726.
5. Green PUR, Glickman RM. Intestinal lipoprotein metabolism. J Lipid Res. 1981;22:1153-1173.
6. Ockner RK, Isselbacher KJ. Recent concepts of intestinal fat absorption. Rev Physiol Biochem Pharmacol. 1974;107-146.
7. Tso P, Drake DS, Black DD, Sabesin SM. Evidence for separate pathways of chylomicron and very low-density lipoprotein assembly and transport by rat small intestine. Am J Physiol. 1984;247:G599-G610.
8. Ockner RK, Hughes FB, Isselbacher KJ. Very low density lipoproteins in intestinal lymph: origin, composition, and role in lipid transport in the fasting state. J Clin Invest. 1969;48:2079-2088.
9. Hussain MM, Click JM, Rothblat GH. In vitro model systems: cell cultures used in lipid and lipoprotein research. Curr Opin Lipidol. 1992;3:173-178.
10. Dashti N. Synthesis and secretion of nascent lipoprotein particles. Prog Lipid Res. 1991;30:219-230.
11. Field FJ, Mathur SN. Intestinal lipoprotein synthesis and secretion. Prog Lipid Res. 1995;34:185-198.
12. Hillgren KM, Kato A, Borchardt RT. In vitro systems for studying intestinal drug absorption. Med Res Rev. 1995;15:83-109.
13. Moberly JB, Cole TG, Schonfeld G. Oleic acid stimulation of apolipoprotein B secretion from HepG2 and Caco-2 cells occurs post-transcriptionally. Biochim Biophys Acta. 1990;1042:70-80.
14. Van Greevenbroek MMJ, Voorhout WF, Erkelens DW, Van Meer G, De Bruin TWA. Palmitic acid and linoleic acid metabolism in Caco-2 cells: different triglyceride synthesis and lipoprotein secretion. J Lipid Res. 1995;36:13-24.
15. Dashti N, Smith EA, Alaupovic P. Increased production of apolipoprotein B and its lipoproteins by oleic acid in Caco-2 cells. J Lipid Res. 1990;31:113-123.
16. Traber MG, Kayden HJ, Rindler MJ. Polarized secretion of newly synthesized lipoproteins by the Caco-2 human intestinal cell line. J Lipid Res. 1987;28:1350-1363.
17. Reisher SR, Hughes TE, Ordovas JM, Schaefer EJ, Feinstein SI. Increased expression of apolipoprotein genes accompanies differentiation in the intestinal cell line Caco-2. Proc Natl Acad Sci U S A. 1993;90:5757-5761.
18. Wetterau JR, Aggerbeck LP, Bouma M-E, Eisenberg C, Munck A, Hermier M, Schmitz J, Gay G, Rader DJ, Gregg RE. Absence of microsomal triglyceride transfer protein in individuals with abetalipoproteinemia. Science. 1992;258:999-1001.
19. Yao Z, Blackhart BD, Johnson DF, Taylor SM, Haubold KW, McCarthy BJ. Elimination of apolipoprotein B48 formation in rat hepatoma cell lines transfected with mutant human apolipoprotein B cDNA constructs. J Biol Chem. 1992;267:1175-1182.