Beyond tritiated thymidine: Flow cytometric assays for the evaluation of lymphocyte activation/proliferation

Clinical Immunology Newsletter

Volumn 16, Issue 12, 1996, Pages 164-172

  O'Gorman, Maurice R G ^a   Corrochano, Virginna ^a   Poon, Rebecca Y M ^b  

^a NORTHWESTERN UNIVERSITY   (United States)

^b Sigma Chemical Company   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0006926399     PISSN: 01971859     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0197-1859(97)86179-1     Document Type: Article

References (25)

1. Basse P, Herberman RB, Wasserman K, Goldfarb RH: Selective accumulation of adoptively transferred A-LAK cells in metastases. J Exp Med 74(2):479, 1990.
2. Constant SL, Wilson RA: In vivo lymphocyte responses in the draining lymph nodes of mice exposed to Schistosome mansoni: Preferential proliferation of T cells is central to the induction of protective immunity. Cell Immunol 139:145, 1992.
3. Cost DM, Fineman D, Steger S: A flow cytometry-based screening assay for lymphocyte proliferation. Clin Immunol Newslett 13:82-85, 1993.
4. Dailey SW, Rose DG, Carabasi RA, Ahlswede K, Williams SK: Origin of cells that line damaged native blood vessels following endothelial cell transplantation. Am J Surg 162:107, 1991.
5. Decker T, Lohmann-Amthes M-L: A quick and simple method for the quantitation of lactate dehydrogenase release in measurement of cellular cytotoxicity and tumor necrosis factor (TNF) activity. J Immunol Methods 15:61-69, 1988.
6. Forster I, Vieira P, Rajewsky K: Flow cytometric analysis of cell proliferation dynamics in the B cell compartment of the mouse. Int Immunol 1:321-331, 1989.
7. Hara T, Jung LKL, Bjorndahl JM, Fu SM: Human T cell activation III. Rapid induction of a phosphorylated 28kD/32kD disulfide-linked early activation antigen (EA 1) by 12-O-tetradecanoyl phorbol-13-acetate, mitogens, and antigens. J Exp Med 164:1988-2005, 1986.
8. Horan PK, Slezak SE: Stable cell membrane labeling. Nature 340:167-168, 1989.
9. Janossy G, Borthwick N, Lomnitzer R, Medina E, et al.: Lymphocyte activation in HIV-1 infection. I. Predominant proliferative defects among CD45RO+ cells of the CD4 and CD8 lineages. AIDS 7 (Suppl. 1):S133-144, 1995.
10. Jensen BD, Schmitt TC, Slezak SE: Labeling of mammalian cells for in vivo cell tracking by a fluorescence method. Prog Clin Biol Res 355:199, 1990.
11. Johannisson A, Thuvander A, Gadhasson I-L: Activation markers and cell proliferation as indicators of toxicity: A flow cytometric approach. Cell Biol Toxicol 11:355-366, 1995.
12. Krowka JF, Cuevas B, Maron DC, et al.: Expression of CD69 after in vitro stimulation: A rapid method for quantitating impaired lymphocyte responses in HTV-infected individuals. J Acquir Immune Defic Syndr Hum Retrovirol 11:95-104, 1996.
13. Krowka JF, Sarin S, Namikawa R, et al.: Human T cells in the SCID-hu mouse are phenotypically normal and functionally competent. J Immunol 146:3751-3756, 1991.
14. Lanier LL, Buck DW, Rhodes L, et al.: Interleukin 2 activation of natural killer cells rapidly induces the expression and phosphorylation of the Leu-23 activation antigen. J Exp Med 167:1572-1585, 1988.
15. Maino VC, Suni MA, Ruitenberg JJ: Rapid flow cytometric method for measuring lymphocyte subset activation. Cytometry 20:127-133, 1995.
16. Mossman T: Rapid colorimetric assay for cellular growth and survival: application and cytotoxicity assays. J Immunol Methods 65:55-63, 1983.
17. Neckers LM, Funkhouser WK, Trepel JB, Chessman J, Graztzner HG: significant non-S phase DNA synthesis visualized by flow cytometry in activated and in malignant human lymphoid cells. Exp Cell Res 156:429-438, 1985.
18. Paxton H, Cunningham Rundles S, O'Gorman MRG: Laboratory evaluation of the cellular immune system. In Clinical diagnosis and management by laboratory methods, Henry JB, ed. 19th ed. Philadelphia: W.B. Saunders, 1996.
19. Samlowski WE, Robertson BA, Draper BK, Prystas E, McGregor JR: Effects of Supravital fluorochromes used to analyze the in vivo homing of murine lymphocytes. J Immunol Methods 144:101-115, 1991.
20. Pires V, Harab RC, Rumjanek VM, Braz J: Trifluoperazine reduces the expression of CD69 in phytohemagglutinin-activated lymphocytes. Med Biol Res 29:479-483, 1996.
21. Shenker BJ, Rooney C, Vitale L, Shapiro IM: Immunotoxic effects of mercuric compounds on human lymphocytes and monocytes. I. Suppression of T cell activation. Immunopharmacol Immunotoxicol 14:539-553, 1992.
22. Simms PE, Ellis TM: Utility of flow cytometric detection of CD69 expression as a rapid method for determining poly-and oligoclonal lymphocyte activation. Clin Diagn Lab Immunol 3:301-304, 1996.
23. Slezak SE, Horan PK: Fluorescent in vivo tracking of hematopoietic cells, Part I: Technical considerations. Blood 74(6):2171-2177, 1989.
24. Yamamura Y, Rodriguez N, Schwartz A, Eylar E, Yano N: Anti-CD4 cytotoxic lymphocyte (CTL) activity in HIV+ patients: Flow cytometric analysis. Cell Mol Biol 41 (Suppl. 1):S133-S144, 1995.
25. Yamamura Y, Rodriguez N, Schwartz A, Eylar E, Bagwell B, Yano N: A new flow cytometric method for quantitative assessment of lymphocyte mitogen potentials. Cell Mol Biol 41 (Suppl. 1):S121-S132, 1995.