A sensitive ELISPOT assay to detect low-frequency human T lymphocytes

Journal of Immunological Methods

Volumn 210, Issue 2, 1997, Pages 149-166

  McCutcheon, M ^a   Wehner, N ^a   Wensky, A ^a   Kushner, M ^a   Doan, S ^a   Hsiao, L ^a   Calabresi, P ^b   Ha, T ^a   Tran, T V ^a   Tate, K M ^a   Winkelhake, J ^a   Spack, E G ^a  

^a GLAXOSMITHKLINE   (United States)

^b NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE   (United States)

Author keywords

Autoantigen; Cytokine; ELISPOT; Human

Indexed keywords

GAMMA INTERFERON; INTERLEUKIN 2;

ARTICLE; AUTOANALYSIS; CELL ISOLATION; CELL SELECTION; COMPUTER ANALYSIS; CONTROLLED STUDY; HUMAN; HUMAN CELL; NORMAL HUMAN; PRIORITY JOURNAL; T LYMPHOCYTE ACTIVATION; T LYMPHOCYTE SUBPOPULATION;

AMINO ACID SEQUENCE; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; INTERLEUKIN-2; LYMPHOKINES; MOLECULAR SEQUENCE DATA; SENSITIVITY AND SPECIFICITY; T-LYMPHOCYTES;

EID: 0031590668     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00182-8     Document Type: Article

References (18)

1. Cui Y., Chang L.-J. Computer-assisted, quantitative cytokine enzyme-linked immunospot analysis of human immune effector cell function. BioTechniques. 22:1997;1146.
2. Czerkinsky C., Andersson G., Ekre H.-P., Nilsson L.-A., Klareskog L., Ouchterlony Ö. Reverse ELISPOT assay for clonal analysis of cytokine production. J. Immunol. Methods. 110:1988;29-36.
3. ElGhazali G.E.B., Paulie S., Andersson G., Hansson Y., Holmquist G., Sun J.-B., Olsson T., Ekre H.P., Troye-Blomberg M. Number of interleukin-4- and interferon-γ-secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutinin: distinct response profiles depending on the type of antigen used for activation. Eur. J. Immunol. 23:1993;2740-2745.
4. Herr W., Linn B., Leister N., Wandel E., Meyer zum Buschenfelde K.H., Wolfel T. The use of computer-assisted video image analysis for the quantification of CD8+ T lymphocytes producing tumor necrosis factor alpha spots in response to peptide antigens. J. Immun. Meth. 203:1997;141.
5. Jung T., Schauer U., Heusser C., Neumann C., Rieger C. Detection of intracellular cytokines by flow cytometry. J. Immunol. Meth. 159:1993;197.
6. Link H., Sun J.-B., Lu C.-Z., Xiao B.-G., Fredrickson S., Höjeberg B., Olsson T. Myasthenia gravis: T and B cell reactivities to the β-bungarotoxin binding protein presynaptic membrane receptor. J. Neurol. Sci. 109:1992;173-181.
7. Link J., Fredrikson S., Söderström M., Olsson T., Höjeberg B., Ljungdahl Å., Link H. Organ-specific autoantigens induce transforming growth factor-β mRNA expression in mononuclear cells in multiple sclerosis and myasthenia gravis. Ann. Neurol. 35:1994;197.
8. Link J., Söderström M., Ljungdahl Å., Höjeberg B., Olsson T., Xu Z., Fredrikson S., Wang Z.-Y., Link H. Organ-specific autoantigens induce interferon-gamma and interleukin-4 mRNA expression in mononuclear cells in multiple sclerosis and myasthenia gravis. Neurol. 44:1994;728.
9. Maino V.C., Suni M.A., Ruitenberg J.J. Rapid flow cytometric method for measuring lymphocyte subset activation. Cytometry. 20:1995;127.
10. Manz R., Assenmacher M., Pflüger E., Miltenyi S., Radbruch A. Analysis and sorting of live cells acording to secreted molecules, relocated to a cell-surface affinity matrix. Proc. Natl. Acad. Sci. USA. 92:1995;1921.
11. Matsuo H., Batocchi A.P., Hawke S., Nicolle M., Jacobson L., Vincent A., Newsom-Davis J., Willcox N. Peptide-selected T cell lines from myasthenia gravis patients and controls recognize epitopes that are not processed from whole acetylcholine receptor. J. Immunol. 155:1995;3683.
12. Olsson T., Zhi W.W., Höjeberg B., Kostulas V., Yu-Ping J., Anderson G., Ekre H.-P., Link H. Autoreactive T lymphocytes in multiple sclerosis determined by antigen-induced secretion of interferon-γ J. Clin. Invest. 86:1990;981-985.
13. Plebanski M., Burtles S.S. In vitro primary responses of human T cells to soluble protein antigens. J. Immunol. Meth. 170:1994;15.
14. Rönnelid J., Klareskog L. A comparison between ELISPOT methods for the detection of cytokine producing cells: greater sensitivity and specificity using ELISA plates as compared to nitrocellulose membranes. J. Immunol. Methods. 200:1997;17-26.
15. Schielen P., van Rodijnen W., Tekstra J., Albers R., Seinen W. Quantitation of natural antibody producing B cells in rats by an improved ELISPOT technique using the polyvinylidene difluoride membrane as the solid support. J. Immunol. Methods. 188:1995;33.
16. Sharrock C.E.M., Kaminski E., Man S. Limiting dilution analysis of human T cells: a useful clinical tool. Immunol. Today. 11:1990;281.
17. Söderström M., Link H., Sun J.-B., Fredrikson S., Kostulas V., Höjeberg S., Li B.-L., Olsson T. T cells recognizing multiple peptides of myelin basic protein are found in blood and enriched in cerebrospinal fluid in optic neuritis and multiple sclerosis. Scand. J. Immunol. 37:1993;355-368.
18. Söderström M., Link H., Fredrikson H., Sun J.-B. Optic neuritis and multiple sclerosis: the T cell repertoires to myelin proteins and MBP peptides change with time. Acta Neurol. Scand. 90:1994;10-18.