Activation of a β2-adrenergic Receptor/Gsα fusion protein elicits a desensitization-resistant cAMP signal capable of inhibiting proliferation of two cancer cell lines

Receptors and Channels

Volumn 5, Issue 1, 1997, Pages 41-51

  Bertin, Brigitte ^a   Jockers, Ralf ^a   Strosberg, A Donny ^a   Marullo, Stefano ^a  

^a INSTITUT COCHIN   (France)

Author keywords

2 Adrenergic; Cell proliferation; Desensitization; Downregulation; Fusion protein; Gs protein; MAP kinase; Oncogene ras; Receptor sequestration

Indexed keywords

ADENYLATE CYCLASE; BETA 2 ADRENERGIC RECEPTOR; BETA 2 ADRENERGIC RECEPTOR STIMULATING AGENT; CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; FORSKOLIN; HYBRID PROTEIN; ISOPRENALINE; PROTEIN SUBUNIT; RAS PROTEIN; SALMETEROL; STIMULATORY GUANINE NUCLEOTIDE BINDING PROTEIN;

ARTICLE; BINDING SITE; CANCER CELL CULTURE; CARCINOMA CELL; CELL GROWTH; CELL PROLIFERATION; CONTROLLED STUDY; DESENSITIZATION; ENZYME ACTIVATION; ENZYME ACTIVITY; HUMAN; HUMAN CELL; LYMPHOMA CELL; ONCOGENE RAS; PRIORITY JOURNAL; RECEPTOR UPREGULATION;

ADENYLATE CYCLASE; ADRENERGIC BETA-AGONISTS; ALBUTEROL; ANIMALS; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CELL DIVISION; CYCLIC AMP; DOWN-REGULATION; FORSKOLIN; GENE EXPRESSION REGULATION, NEOPLASTIC; GTP-BINDING PROTEIN ALPHA SUBUNITS, GS; IODOCYANOPINDOLOL; ISOPROTERENOL; MICE; PINDOLOL; RAS PROTEINS; RECEPTORS, ADRENERGIC, BETA-2; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0030793889     PISSN: 10606823     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Agbotounou, W., Mousset, M., Piperno, S., Pierre, M., Jacquemin-Sablon. A. and Pierre, J. (1994) Biochem. Pharmacol.
2. Alousi, A. A., Jasper, J. R., Insel, P. A and Motulsky, H. J. (1991) FASEB J., 5, 2300-2303
3. Benovic, J, L., Bouvier, M., Caron, M. and Lefkowitz, R. J. (1988) Annual Review of Cellular Biology 4, 405-427.
4. Benovic, J. L., DeBlasi, A., Stone, W. C., Caron, M. G. and Lefkowitz, R. J. (1989) Science, 246, 235-240.
5. Bertin, B., Freissmuth, M., Jockers, R., Strosberg, A. D. and Marullo, S. (1994) Proc. Natl. Acad. Sci. USA, 91, 8827-8831.
6. Bourne, H. R., Coffins, P., Melmon, K. L., Tomkins, G. M. and Weinstein, Y. (1975) Adv. Cycl. Nucl. Res., 5. 771-786.
7. Bouvier, M., Collins, S., O'Dowd, B. F., Campbell, P. T., de Biasi, A., Kobilka, B. K., MacGregor, C., Irons, G. P., Caron, M. G. and Lefkowitz, R. J. (1989) J. Biol. Chem., 264, 16786-16792.
8. Buchmann, A., Ruggeri, B., Klein-Szanto, A. J. and Balmain, A. (1991) Cancer Res., 51, 4097-4101
9. Chen, J. and Iyengar, R. (1994) Science, 263, 1278-1281.
10. Doss, R. C., Perkins, J. P. and Harden, T. K. (1981) J. Biol. Chem., 256, 12281-12286.
11. Gonzales, J. M., O'Donnel, J. K., Stadel, J. M., Sweet, R. W. and Molinoff, P. B. (1992) J. Neurochem., 58, 1093-1103.
12. Ferguson, S. S. G., Ménard, L., Colapietro, A. M., Barak, L. S., Koch, W. J. and Caron, M. G. (1995) J. Biol. Chem., 270, 24782-24789.
13. Ferguson, S. S. G., Downey, W. E., Colapietro, A. M., Barak, L. S., Ménard, L. and Caron, M. G. (1996) Science, 271, 363-366.
14. Hadcock, J. R. and Malbon, C. (1988) Proc. Natl. Acad. Sci. USA. 85, 5021-5025.
15. Hausdorff, W. P., Bouvier, M., O'Dowd, B. F., Irons, G. P., Caron, M. G. and Lefkowitz, R. J. (1989) J. Biol. Chem., 264, 12657-12665.
16. Landis, C. A., Masters, S. B., Spada, A., Pace, A. M., Bourne, H. R. and Vallar, L. (1989) Nature, 340, 692-696.
17. Lefkowitz, R. J., Hausdorff, W. P. and Caron, M. G. (1990) Trends Pharmacol. Sci., 11, 190-194.
18. Leiber, D., Jasper, J. R., Alousi, A. A., Martin, J., Bernstein, D. and Insel, P. A. (1993) J. Biol. Chem., 268, 3833-3837.
19. Lohse, M. J , Benovic, J. L., Caron, M. G. and Lefkowitz, R. J. (1990) J. Biol. Chem., 265, 3202-3209.
20. Lohse, M. J., Benovic, J. L., Codina, J., Caron, M. G. and Lefkowitz, R. J. (1990) Science, 248, 1547-1550.
21. Lyons, J., Landis, C. A., Harsh, G., Vallar, L., Grünewald, K., Feichtinger, H., Duh, Q.-Y., Clark, O. H., Kawasaki, E., Bourne, H. R. and McCormick, F. (1990) Science, 249, 655-659.
22. Mahan, L. C., Koachman, A. M. and Insel, P. A. (1985) Proc. Natl. Acad. Sci. USA, 82, 129-133.
23. Milligan, G. (1993) Trends in Pharmacol. Sci., 14, 413-418.
24. Mosmann, J. (1983) J. Immunol Meth., 65, 55.
25. Nantel, F., Bouvier, M., Strosberg, A. D. and Marullo, S. (1995) Br. J. Pharmacol., 114, 1045-1051.
26. Nantel, F., Marullo, S., Krief, S., Strosberg, A D. and Bouvier, M. (1994) J. Biol. Chem., 269, 13148-13155.
27. Okamoto, T., Murayama, Y., Hayashi, Y., Inagaki, M., Ogata, E. and Nishimoto, I. (1991) Cell 67, 723-730.
28. Parker, E. M., Swigart, P., Numally, M. H., Perkins, J. P. and Ross, E. M. (1995) J. Biol. Chem., 270, 6482-6487.
29. Pippig, S., Andexinger, S. and Lohse, M. J. (1995) Mol. Pharmacol., 47, 666-676.
30. Seuwen, K. and Pouysségur, J. (1992) Adv. in Cancer Res., 58, 75-93.
31. Sibley, D. R., Strasser, R. H., Benovic, J. L., Daniel, K. and Lefkowitz, R. J. (1986) Proc. Natl. Acad. Sci. USA, 83, 9408-9412.
32. Valiquette, M., Bonin, H., Hnatowich, M., Caron, M. G., Lefkowitz, R. J. and Bouvier, M. (1990) Proc. Natl. Acad. Sci. USA, 87, 5089-5093.
33. Yu. S. S., Lefkowitz, R. J. and Hausdorff, W. P. (1993) J. Biol. Chem., 268, 337-341.