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Volumn 279, Issue 5358, 1998, Pages 1922-1925

Partial hormone resistance in mice with disruption of the steroid receptor coactivator-1 (SRC-1) gene

Author keywords

[No Author keywords available]

Indexed keywords

ESTROGEN; PROGESTERONE; STEROID HORMONE; STEROID RECEPTOR; TESTOSTERONE; TRANSACTIVATOR PROTEIN;

EID: 0032549744     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5358.1922     Document Type: Article
Times cited : (602)

References (29)
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    • The GLVP cassette encodes a transcriptional regulator composed of the activation domain of VP-16, the DNA binding domain of gal4, and the mutated ligand-binding domain of PR. This regulator can be activated only by a progesterone antagonist, RU-486 [Y. Wang, J. Xu, T. Pierson, B. W. O'Malley, S. Y. Tsai, Gene Ther. 4, 432 (1997)]. This RU486-dependent transregulator could control a transgene containing a gal4-binding site. The GLVP regulator was incorporated in case the SRC-1 mutation resulted in a lethal phenotype.
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    • note
    • Phage clones were identified by screening a 129Sv mouse genomic DNA library with a human SRC-1 cDNA probe. Inserts were excised by Not I digestion and subcloned into pBluescript II SK plasmid (Stratagene). Regions corresponding to specific amino acid sequences were defined by sequencing or by hybridization with oligonucleotide probes.
  • 29
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    • note
    • We thank K. Bramlett and L. A. Hadsell for technical assistance; C. Funk, Y. Wang, Z-Q. Ma, F. A. Pereira, and N. McKenna for discussion; P. Soriano for the mouse genomic library; A. Bradley for ES cells; D. P. Edwards for the SRC-1 antibody; P. Chambon for the TIF2 cDNA; and J. D. Chen for the RAC3 cDNA. Supported by a NIH NRSA fellowship (J.X.) and NIH grants (B.W.O.).


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