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6 cells, respectively, in the tail vein. Phenotypic analysis of spleen and lymph node cells was performed with mAbs specific for the clonotypic Des-TCR (Désiré-1) (6) and for mouse CD8 (53-6.7, Pharmingen).
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note
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b and Des-TCR mice have been described (4). All mice were kept under specific pathogen-free conditions at the central animal facility of the German Cancer Research Center. Tumor growth was evaluated as described (4).
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18
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note
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b transgene-expressing keratinocytes could be seen by immunofluorescence histology, as judged by the absence of infiltrating Des-TCR T cells in the thoracic wall skin from the grafted and contralateral side.
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19
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note
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b was performed at the age of 6 weeks.
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20
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3643052993
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data not shown
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J. Alferink, A. Tafuri, D. Vestweber, R. Hallmann, G. J. Hämmerling, B. Arnold, data not shown.
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3643081323
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note
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-/- mice (eight mice per group) rejected the transplanted skin (median survival time, 24 days).
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22
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3643082379
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note
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6 cells) recipients. After whole-body perfusion, the distribution of radioactivity in various organs was determined in a γ-counter. Radiolabeled cells accumulated in neonatal versus adult skin in ratios of 8.41:1, 6.22:1, and 10:1 in three independent experiments.
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24
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3643138570
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note
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b mice. After 4 hours, whole-body perfusion was performed and the number of CFSE-labeled cells was determined by immunohistological analysis of the skin. CFSE-positive cells were enriched in neonatal skin and were only rarely visible in adult skin.
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-/- mice; J. Schöffel, A. Klevenz, S. Schmitt, and G. Küblbeck for excellent technical assistance; E. Armandola, T. Sacher, and A. Forde for critical reading of the manuscript; A. Frenznick, C. Ley, and S. Hartmann for animal husbandry; S. Copson for help with the manuscript; and G. Moldenhauer for providing control antibody. Supported by grants from Deutsche Forschungsgemeinschaft (SFB 405), the European Union (BIO4-CT-960077 and BIO4-97-2151), and VW-Stiftung (I/72422).
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