Neonatal tolerance revisited: Turning on newborn T cells with dendritic cells

Science

Volumn 271, Issue 5256, 1996, Pages 1723-1726

  Ridge, John Paul ^a   Fuchs, Ephraim J ^a,b   Matzinger, Polly ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL EXPERIMENT; ANTIGEN PRESENTING CELL; ARTICLE; DENDRITIC CELL; FEMALE; IMMUNE SYSTEM; IMMUNIZATION; IMMUNOLOGICAL TOLERANCE; MOUSE; NEWBORN; NONHUMAN; PRIORITY JOURNAL; T LYMPHOCYTE;

ANIMALIA;

EID: 0029991928     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5256.1723     Document Type: Article

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62. We obtained the enriched dendritic cell preparations by incubating B6 male spleen cells in Iscove's modified Dulbecco's medium plus 10% fetal bovine serum in Falcon tissue culture dishes (number 3025) for 2 hours at 37°C, removing nonadherent cells, and incubating the remaining adherent cells overnight at 37°C in medium containing mouse recombinant granulocyte-macrophage colony-stimulating factor (5 ng/ml) (Pharmingen, San Diego, CA). Nonadherent cells were then harvested and further purified over a 50% Percoll density gradient. By flow cytometery, the cell populations were typically bright for MHC class II staining and contained <2% T cells, <5% B cells, and >80 to 90% dendritic cells, as seen by staining for the dendritic cell markers 33D1 and N418.
63. We thank L. Yuan I for suggesting the day 7 tolerance experiment; A. Bendelac for other useful sug gestions and encouragement, and A. Bendelac, L Chiodetti, L. D'Adamio, M. Epstein, R. Germain, R. Schwartz, and A. Sher for reading the manuscript We especially thank B Tineo and C. Fenton for caring for the 351 baby mice and their mothers reported here, as well as several hundred mice that did not make it into these pages.