Analysis of the catalytic mechanism of a fungal lipase using computer-aided design and structural mutants

Protein Engineering

Volumn 9, Issue 6, 1996, Pages 507-517

  Beer, H D ^a   Wohlfahrt, G ^a   McCarthy, J E G ^a   Schomburg, D ^a   Schmid, R D ^b  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Enzyme kinetics; Homology modelling; Molecular mechanics; Protein variants; Reaction mechanism; Rhizopus oryzae lipase; Structure function relationship

Indexed keywords

AMINO ACID; TRIACYLGLYCEROL LIPASE;

ARTICLE; CATALYSIS; COMPUTER AIDED DESIGN; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME STRUCTURE; GENE; HYDROLYSIS; LIPID METABOLISM; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; RHIZOPUS;

AMINO ACID SEQUENCE; BINDING SITES; CATALYSIS; CLONING, MOLECULAR; COMPUTER SIMULATION; COMPUTER-AIDED DESIGN; FUNGAL PROTEINS; GENES, FUNGAL; HYDROLYSIS; LIPASE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; RHIZOPUS; SEQUENCE ALIGNMENT; SPECIES SPECIFICITY;

ESCHERICHIA COLI; RHIZOPUS; RHIZOPUS ORYZAE;

EID: 0029744652     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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