A model for the mechanism of human topoisomerase I

Science

Volumn 279, Issue 5356, 1998, Pages 1534-1541

  Stewart, Lance ^a,c   Redinbo, Matthew R ^a   Qiu, Xiayang ^a,d   Hol, Wim G J ^a   Champoux, James J ^b  

^a UNIVERSITY OF WASHINGTON   (United States)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^c Emerald BioStructures Inc   (United States)

^d GLAXOSMITHKLINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA TOPOISOMERASE;

AMINO TERMINAL SEQUENCE; ARTICLE; DNA DETERMINATION; DNA REPLICATION; ENZYME ANALYSIS; ENZYME MECHANISM; HUMAN; HUMAN CELL; PRIORITY JOURNAL; STRUCTURE ANALYSIS; THREE DIMENSIONAL IMAGING; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ARGININE; BINDING SITES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; DNA; DNA TOPOISOMERASES, TYPE I; HUMANS; HYDROGEN BONDING; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; OLIGODEOXYRIBONUCLEOTIDES; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; TYROSINE;

EID: 0032489675     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5356.1534     Document Type: Article

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20. 632 of topo l in the recombinases remains to be determined.
21. 632 of topo l in the recombinases remains to be determined.
22. 632 of topo l in the recombinases remains to be determined.
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42. We thank G. C. Ireton, E. Pohl, S. Turley, B. Bernstein, C. Verlinde, F. Athappilly, F. van den Akker, D. Chudzik, J. Yeh, and R. Li for their technical assistance and many engaging conversations; and Y. Pocker for helpful comments concerning the catalytic mechanism; H. Bellamy and P. Kuhn at the Stanford Synchrotron Radiation Laboratory for help with MAD and isomorphous data collection; and the staffs of Brookhaven National Laboratory, the Cornell High Energy Synchrotron Source, and the European Synchrotron Radiation Facility for their support with data collection. This work was supported by grant GM49156 from NIH (to J.J.C.), by American Cancer Society grant PF-3905 (to L.S.), by an NIH fellowship grant GM16713 to X.Q., and by a major equipment grant from the Murdock Charitable Trust to the Biomolecular Structure Center and grant CA65656 from NIH (to W.G.J.H).