Analysis of mutagens with single cell gel electrophoresis, flow cytometry, and forward mutation assays in an isolated clone of Chinese hamster ovary cells

Environmental and Molecular Mutagenesis

Volumn 32, Issue 4, 1998, Pages 360-368

  Wagner, Elizabeth D ^a   Rayburn, A Lane ^b   Anderson, Diana ^b   Plewa, Michael J ^a,c  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b BIBRA INTERNATIONAL   (United Kingdom)

^c 364 Environmental and Agricultural Sciences Building ^*  (United States)

Author keywords

2 Acetoxyacetylaminofluorene; Calibration; Comet assay; Ethyl methanesulfonate; Ultraviolet radiation

Indexed keywords

MUTAGENIC AGENT;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; CYTOTOXICITY; DNA DETERMINATION; DNA STRAND BREAKAGE; FLOW CYTOMETRY; GEL ELECTROPHORESIS; GENE MUTATION; MUTAGEN TESTING; NONHUMAN; TOXICITY TESTING;

ACETOXYACETYLAMINOFLUORENE; ANIMALS; CHO CELLS; CLONE CELLS; CRICETINAE; CRICETULUS; ELECTROPHORESIS, AGAR GEL; ETHYL METHANESULFONATE; FLOW CYTOMETRY; MUTAGENICITY TESTS; MUTAGENS; TRANSFERASES (OTHER SUBSTITUTED PHOSPHATE GROUPS); ULTRAVIOLET RAYS;

EID: 0032446271     PISSN: 08936692     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2280(1998)32:4<360::AID-EM10>3.0.CO;2-T     Document Type: Article

References (24)

1. Andersen D, Plewa MJ (1998): The International Cornet Assay Workshop. Mutagenesis 13:67-73.
2. Anderson D, Yu T-W, Browne MA (1997): The use of the same image analysis system to detect genetic damage in human lymphocytes treated with doxorubicin in the comet and fluorescence in situ hjbridisatipn (FISH) assays. Mutât Res 390:69-77.
3. Bastlova T, Vodicka P, Peterkova K, Hemminki K, Lambert B (1995): Styrène oxide-induced HPRT mutations, DNA adducts and DNA strand breaks in cultured human lymphocytes. Carcinogenesis 16:2357-2362.
4. Bickham JW, Sawin VL, Burton DW, McBee K (1992): Flow-cytometric analysis of the effects of triethylenemelamine on somatic and testicular tissues of the rat. Cytometry 13:368-373.
5. Bickham JW, Sawin VL, McBee K, Smolen MJ, Derr JN (1994): Further flow cytometric studies of the effects of triethylenemelamine on somatic and testicular tissues of the rat. Cytometry 15:222-229.
6. Biradar DP, Rayburn AL (1995): Flow cytogenetic analysis of whole cell clastogenicity of herbicides found in groundwater. Arch Environ Contam Toxicol 28:13-17.
7. Fairbairn DW, Olive PL, O'Neill KL (1995): The comet assay: A comprehensive review. Mutât Res 339:37-59.
8. Gichner T, Plewa MJ (1998): Induction of somatic DNA damage as measured by single cell gel electrophoresis and point mutation in leaves of tobacco plants. Mutât Res 401:143-152.
9. Grogan WM, Collins JM (1990): Guide to flow cytometry methods. New York: Marcel Dekker.
10. Hanelt S, Heibig R, Hartmann A, Lang M, Seidel A, Speit G (1997): A comparative investigation of DNA adducts, DNA strand breaks and gène mutations induced by benzo[α]pyrene and (±)-antibenzo[α]pyrene-7,8-diol 9,10-oxide in cultured human cells. Mutât Res 390:179-188.
11. Hartmann A, Speit G (1995): Genotoxic effects of chemicals in the single cell gel (SCG) test with human blood cells in relation to the induction of sister-chromatid exchanges (SCE). Mutât Res 346:49-56.
12. Majeska JB, Holden HE (1995): Genotoxic effects of p-aminophenol in Chinese hamster ovary and mouse lymphoma cells: Results of a multiple endpoint test. Environ Mol Mutagen 26:163-170.
13. McKelvey-Martin VJ, Green MHL, Schmezer P, Pool-Zobel BL, De Meo MP, Collins A (1993): The single cell gel electrophoresis assay (comet assay): A European review. Mutât Res 288:47-63.
14. Monteith DK, Vanstone J (1995): Comparison of the microgel electrophoresis assay and other assays for genotoxicity in the detection of DNA damage. Mutât Res 345:97-103.
15. Otto FJ, Oldiges H (1980): Flow cytometric studies in chromosomes and whole cells for the detection of clastogenic effects. Cytometry 1:13-17.
16. Otto FJ, Oldiges H, Gohde W, Jain VK (1981): Flow cytometric mea-surement of nuclear DNA content variations as a potential in vivo mutagenicity test. Cytometry 2:189-191.
17. Plewa MJ, Wagner ED, Kirchoff L, Repetny K, Adams LC, Raybum AL (1998): The use of single cell gel electrophoresis and flow cytometry to identify antimutagens from commercial soybean by-products. Mutât Res 402:211-218.
18. Singh NP, Tice RR, Stephens RE, Schneider EL (1991): A microgel electrophoresis technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides. Mutat Res 252:289-296.
19. Slamenova D, Gabelova A, Ruzekova L, Chalupa I, Horvathova E, Farkasova T, Bozsakyova E, Stetina R (1997): Detection of MNNG-induced DNA lesions in mammalian cells; validation of comet assay against DNA unwinding technique, alkaline elution of DNA and chromosomal aberrations. Mutât Res 383:243-252.
20. Speit G, Hanelt S, Heibig R, Seidel A, Hartmann A (1996): Detection of DNA effects in human cells with the comet assay and their relevance for mutagenesis. Toxicol Lett 88:91-98.
21. Tice RR (1995): The single cell gel/comet assay: A microgel electropho-retic technique for the detection of DNA damage and repair in individual cells. In Phillips DH, Venitt S (eds). Environmental mutagenesis. Oxford:Bios Scientific Publishers, pp 315-339.
22. Tindall KR, Stankowski LF Jr (1989): Molecular analysis of spontaneous mutations at the gpt locus in Chinese hamster ovary (AS52) cells. Mutat Res 220:241-2,53.
23. Vodicka P, Bastlova T, Vodickova L, Peterkova K, Lambert B, Hemminki K (1995): Biomarkers of styrène exposure in lamination workers: Levels of O6-guanine DNA adducts, DNA strand breaks and mutant frequencies in the hypoxanthine guanine phosphoribosyltransferase gene in T-lymphocytes. Carcinogenesis 16: 1473-1481.
24. Wagner ED, Rayburn AL, Anderson D, Plewa MJ (1998): Calibration of the single cell gel electrophoresis assay, flow cytometry analysis and forward mutation in Chinese hamster ovary cells. Mutagenesis 13:81-84.