Calibration of the single cell gel electrophoresis assay, flow cytometry analysis and forward mutation in Chinese hamster ovary cells

Mutagenesis

Volumn 13, Issue 1, 1998, Pages 81-84

  Wagner, Elizabeth D ^a   Rayburn, A Lane ^a   Anderson, Diana ^b   Plewa, Michael J ^a,c  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b BIBRA INTERNATIONAL   (United Kingdom)

^c 364 Environ and Agric Sci Bldg ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL NUCLEUS DNA; DNA; MESYLIC ACID ETHYL ESTER; TIOGUANINE;

ANIMAL CELL; ARTICLE; ASSAY; CALIBRATION; CHO CELL; CHROMOSOME DAMAGE; CONTROLLED STUDY; DNA DAMAGE; DNA DETERMINATION; DNA STRAND BREAKAGE; EXCISION REPAIR; FLOW CYTOMETRY; GEL ELECTROPHORESIS; GENE; GENE MUTATION; GENOTOXICITY; KINETICS; MUTAGEN TESTING; MUTATION RATE; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; CALIBRATION; CELL SURVIVAL; CHO CELLS; CLONE CELLS; CRICETINAE; DNA DAMAGE; ELECTROPHORESIS, AGAR GEL; ETHYL METHANESULFONATE; FLOW CYTOMETRY; MUTAGENICITY TESTS; POINT MUTATION; THIOGUANINE;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; MAMMALIA;

EID: 0031982757     PISSN: 02678357     EISSN: None     Source Type: Journal    
DOI: 10.1093/mutage/13.1.81     Document Type: Article

References (13)

1. Anderson, D., Yu, T.-W. and Browne, M.A. (1997) The use of the same image analysis system to detect genetic damage in human lymphocytes treated with doxorubicin in the Comet and fluorescence in situ hybridisation (FISH) assays. Mutat. Res., 390, 69-77.
2. Biradar, D.P. and Rayburn, A.L. (1995) Flow cytogenetic analysis of whole cell clastogenicity of herbicides found in groundwater. Arch. Environ. Contamin. Toxicol., 28, 13-17.
3. Fairbairn, D.W., Olive, P.L. and O'Neill, K.L. (1995) The comet assay: a comprehensive review. Mutat. Res., 339, 37-59.
4. Grogan, W.M. and Collins, J.M. (1990) Guide to Flow Cytometry Methods. Marcel Dekker, New York, NY, 228 pp.
5. Hartmann, A. and Speit, G. (1994) Comparative investigations of the genotoxic effects of metals in the single cell gel (SCG) assay and the sister chromatid exchange (SCE) test. Environ. Mol. Mutagen., 23, 299-305.
6. Hartmann, A. and Speit, G. (1995) Genotoxic effects of chemicals in the single cell gel (SCG) test with human blood cells in relation to the induction of sister-chromatid exchanges (SCE). Mutat. Res., 346, 49-56.
7. Leroy, T., Van Hummelen, P., Anard, D., Castelain, P., Kirsch-Volders, M., Lauwerys, R. and Lison, D. (1996) Evaluation of three methods for the detection of DNA single-strand breaks in human lymphocytes: alkaline elution, nick translation, and single-cell gel electrophoresis. J. Toxicol. Environ. Hlth, 47, 409-422.
8. Majeska, J.B. and Holden, H.E. (1995) Genotoxic effects of p-aminophenol in Chinese hamster ovary and mouse lymphoma cells: results of a multiple endpoint test. Environ. Mol. Mutagen., 26, 163-170.
9. Monteith, D.K. and Vanstone, J. (1995) Comparison of the microgel electrophoresis assay and other assays for genotoxicity in the detection of DNA damage. Mutat. Res., 345, 97-103.
10. Otto, F.J. and Oldiges, H. (1980) Flow cytometric studies in chromosomes and whole cells for the detection of clastogenic effects. Cytometry, 1, 13-17.
11. Plewa, M.J., Wagner, E.D., Kirchoff, L., Repetny, K., Adams, L.C. and Rayburn, A.L. (1997) The use of single cell gel electrophoresis and flow cytometry to identify antimutagens from commercial soybean by-products. Mutat. Res., in press
12. Slameňová, D., Gábelová, A., Ružeková, L., Chalupa, I., Horváthová, E., Farkašová, T., Bozsakyová, E. and Štětina, R. (1997) Detection of MNNG-induced DNA lesions in mammalian cells: validation of comet assay against DNA unwinding technique, alkaline elution of DNA and chromosomal aberrations. Mutat. Res., 383, 243-252.
13. Tindall, K.R. and Stankowski, L.F., Jr (1989) Molecular analysis of spontaneous mutations at the gpt locus in Chinese hamster ovary (AS52) cells. Mutat. Res., 220, 241-253.