Purification and activation of recombinant p38 isoforms α, β, γ and δ

Protein Expression and Purification

Volumn 14, Issue 2, 1998, Pages 221-228

  Keesler, George A ^a   Bray, Jeff ^a   Hunt, John ^a   Johnson, David A ^a   Gleason, Tom ^a   Yao, Zhengbin ^a   Wang, Shen Wu ^a   Parker, Carol ^a   Yamane, Harvey ^a   Cole, Craig ^a   Lichenstein, Henri S ^a  

^a AMGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME ACTIVATION; ENZYME PURIFICATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PROTEIN SERINE THREONINE KINASE;

ESCHERICHIA COLI;

EID: 0032212557     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.0947     Document Type: Article

References (25)

1. Han J., Lee J. D., Tobias P. S., Ulevitch R. J. Endotoxin induces rapid protein tyrosine phosphorylation in 70 Z/3 cells expressing CD14. J. Biol. Chem. 268:1993;25009-25014.
2. Han J., Lee J. D., Bibbs L., Ulevitch R. J. A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. Science. 265:1994;808-811.
3. Lee J. C., Laydon J. T., McDonnell. P. C., Gallagher T. F., Kumar S., Green D., McNulty D., Blumenthal M. J., Heys J. R., Landvatter S. W., Strickler J. E., McLaughlin M. M., Siemens I., Fisher S., Livi G. P., White J. R., Adams J. L., Young P. R. A protein kinase involved in the regulation of inflammatory cytokine biosynthesis. Nature. 372:1994;739-746.
4. Jiang Y., Chen C., Li Z., Guo W., Gegner J. A., Lin S., Han J. Characterization of the structure and function of a new mitogen-activated protein kinase (p38β). J. Biol. Chem. 271:1996;17920-17926.
5. Stein B., Yang M. X., Young D. B., Janknecht R., Hunter T., Murry B. W., Barbosa M. S. p38-2, a novel mitogen-activated protein kinase with distinct properties. J. Biol. Chem. 272:1997;19509-19517.
6. Li Z., Jiang Y., Ulevitch R. J., Han J. The primary structure of p38γ: A new member of the p38 group of MAP kinases. Biochem. Biophys. Res. Commun. 228:1996;334-340.
7. Lechner C., Zahalka M. A., Giot J. F., Moller N. P., Ullrich A. ERK6, a mitogen-activated protein kinase involved in C2C12 myoblast differentiation. Proc. Natl. Acad. Sci. USA. 93:1996;4355-4359.
8. Mertens S., Craxton M., Goedert M. SAP kinase-3, a new member of the family of mammalian stress-activated protein kinases. FEBS Lett. 383:1996;273-276.
9. Wang X. S., Diener K., Manthey C. L., Wang S., Rosenzweig B., Bray J., Delaney J., Cole C. N., Chan-Hui P. Y., Mantlo N., Lichenstein H. S., Zukowski M., Yao Z. Molecular cloning and characterization of a novel p38 mitogen-activated protein kinase. J. Biol. Chem. 272:1997;23668-23674.
10. Goedert M., Cuenda A., Craxton M., Jakes R., Cohen P. Activation of the novel stress-activated protein-kinase SAPK4 by cytokines and cellular stresses is mediated by SKK3 (MKK6): Comparison of its substrate specificity with that of other SAP kinases. EMBO J. 16:1997;3563-3571.
11. Kumar S., McDonnell P. C., Gum R. J., Hand A. T., Lee J. C., Young P. J. Novel homologues of CSBP/p38 MAP kinase: Activation, substrate specificity and sensitivity to inhibition by pyridinyl imidazoles. Biochem. Biophys. Res. Commun. 235:1997;533-538.
12. Jiang Y., Gram H., Zhao M., New L. G., Gu J., Feng L. L., Di Padova F., Ulevitch R. J., Han J. H. Characterization of the structure and function of the fourth member of p38 group mitogen-activated protein kinase, p38δ J. Biol. Chem. 272:1997;30122-30128.
13. Cuenda A., Cohen P., Buee-Scherrer V., Goedert M. Activation of stress-activated protein kinase-3 (SAPK3) by cytokines and cellular stresses is mediated via SAPKK3 (MKK6): Comparison of the specificities of SAPK3 and SAPK2 (RK/p38). EMBO J. 16:1997;295-305.
14. Derijard B., Raingeaud J., Barrett T., Wu I. H., Han J., Ulevitch R. J., Davis R. J. Independent human MAP kinase signal transduction pathways defined by MEK and MKK isoforms. Science. 267:1995;682-685.
15. 22. J. Biol. Chem. 271:1996;26981-26988.
16. Han J., Lee J. D., Jiang Y., Li Z., Feng L., Ulevitch R. J. Characterization of the structure and function of a novel MAP kinase kinase (MKK6). J. Biol. Chem. 271:1996;2886-2891.
17. Raingeaud J., Whitmarsh A. J., Barrett T., Derijard B., Davis R. J. MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway. Mol. Cell. Biol. 16:1996;1247-1255.
18. Moriguchi T., Kuroyanagi N., Yamaguchi K., Gotoh Y., Irie K., Kano T., Shirakabe K., Muro Y., Shibuya H., Matsumoto K., Nishida E., Hagiwara M. A novel kinase cascade mediated by mitogen-activated protein kinase kinase 6 and MKK3. J. Biol. Chem. 271:1996;13675-13679.
19. Cuenda A., Alonso G., Morrice N., Jones M., Meier R., Cohen P., Nebreda A. R. Purification and cDNA cloning of SAPKK3, the major activator of RK/p38 in stress- and cytokine-stimulated monocytes and epithelial cells. EMBO J. 15:1996;4156-4164.
20. Stein B., Brady H., Yang M. X., Young D. B., Barbosa M. S. Cloning and characterization of MEK6, a novel member of the mitogen-activated protein kinase cascade. J. Biol. Chem. 271:1996;11427-11435.
21. Raingeaud J., Gupta S., Rogers J. S., Dickens M., Han J., Ulevitch R. J., Davis R. J. Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine. J. Biol. Chem. 270:1995;7420-7426.
22. Enslen H., Raingeaud J., Davis R. J. Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6. J. Biol. Chem. 273:1998;1741-1748.
23. Ichijo H., Nishida E., Irie K., Tendijke P., Saitoh M., Moriguchi T., Takagi M., Matsumoto K., Miyazono K., Gotoh Y. Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science. 275:1997;90-94.
24. Cai X. Y., Shanahan M., Miller K., Gommoll C., Lundell D., Zavodny P., Dalie B. Expression, purification, and characterization of an activated cytokine-suppressive anti-inflammatory drug-binding protein 2 (CSBP2) kinase from baculovirus-infected insect cells. Protein Expression Purif. 10:1997;263-274.
25. LoGrasso P. V., Frantz B., Rolando A. M., OKeefe S. J., Hermes J. D., O'Neill E. A. Kinetic mechanism for p38 MAP kinase. Biochemistry. 36:1997;10422-10427.