Reporters for the analysis of gene regulation in fungi pathogenic to man

Current Opinion in Microbiology

Volumn 1, Issue 4, 1998, Pages 400-405

  Soll, David R ^a   Srikantha, Thyagarajan ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGI;

CANDIDA ALBICANS; FUNGUS; GENE EXPRESSION REGULATION; GENETICS; HUMAN; KLUYVEROMYCES; MICROBIOLOGY; MYCOSIS; PATHOGENICITY; PROMOTER REGION; REPORTER GENE; REVIEW;

CANDIDA ALBICANS; FUNGI; GENE EXPRESSION REGULATION, FUNGAL; GENES, REPORTER; HUMANS; KLUYVEROMYCES; MYCOSES; PROMOTER REGIONS (GENETICS);

EID: 0032130216     PISSN: 13695274     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1369-5274(98)80056-8     Document Type: Article

References (40)

1. Fonzi WA, Irwin MY: Isogenic strain construction and gene mapping in Candida albicans. Genetics 1993, 134:717-728.
2. Lo HJ, Kohler JR, DiDomenico B, Loebenberg D, Cacciapuoti A, Fink GR: Nonfilamentous C. albicans mutants are avirulent Cell 1997, 90:939-949.
3. Sanglard D, Hube B, Monod M, Odds FC, Gow NA: A triple deletion of the secreted aspartyl proteinase genes SAP4, SAP5, and SAP6 of Candida albicans causes attenuated virulence. Infect Immun 1997, 65:3539-3546.
4. Calera JA, Paris S, Monod M, Hamilton AJ, Debeaupuis JP, Diaquin M, Lopez-Medrano R, Leal F, Latge JP: Cloning and disruption of the antigenic catalase gene of Aspergillus fumigatus. Infect Immun 1997, 65:4718-4724.
5. Kvaal CA, Srikantha T, Soll DR: Misexpression of the white-phase-specific gene WH11 in the opaque phase of Candida albicans affects switching and virulence. Infect Immun 1997, 65:4468-4475.
6. Srikantha T, Klapach W, Lorenz WW, Tsai LK, Laughlin A, Gorman JA, Soll DR: The sea pansy Renilla reniformis luciferase serves as a sensitive bioluminescent reporter for differential gene expression in Candida albicans. J Bacteriol 1996, 178:121-129.
7. Srikantha T, Tsai LK, Soll DR: The WH11 gene of Candida albicans is regulated in two distinct developmental programs through the same transcription activation sequences. J Bacteriol 1997, 179:3837-3844. This paper describes the use of RLUC in the functional characterization of the promoter of the white-phase-specific gene WH11 and the identification of cis-acting sequences involved in the activation of WH11 both in the white-opaque phase transition and the bud-hypha transition.
8. Soll DR: The emerging molecular biology of switching in Candida albicans. ASM News 1997, 62:415-420.
9. Soll DR: Gene regulation during high frequency switching in Candida albicans. Microbiology 1997, 143:279-288.
10. Niedenthal RK, Riles L, Johnston M, Hegemann JH: Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeast. Yeast 1996, 12:773-786.
11. Hampton YR, Koning A, Wright R, Rine J: In vivo examination of membrane protein localization and degradation with green fluorescent protein. Proc Natl Acad Sci USA 1996, 93:828-833.
12. Gould S, Keller G, Schneider M, Howell S, Garrard L, Goldman J, Distel B, Tabak H, Subramani S: Peroxisomal protein import is conserved between yeast, plants, insects, and mammals. EMBO J 1990, 9:85-90.
13. Srikantha T, Chandrasekhar A, Soll DR: Functional analysis of the promoter of the phase-specific WH11 gene of Candida albicans. Mol Cell Biol 1995, 15:1797-1805.
14. Casdaban JM, Martinez-Arias A, Shapira KS, Chou J: β-galactosidase gene fusions for analyzing gene expression in Escherichia coli and yeast. Methods Enzymol 1983, 100:293-308.
15. Nacken V, Achstetter T, Degryse E: Probing the limits of expression levels by varying promoter strength and plasmid copy number in Saccharomyces cerevisiae. Gene 1996, 175:253-260.
16. Ruby SW, Szostak JW, Murray AW: Cloning regulated yeast genes from a pool of LacZ fusions. Methods Enzymol 1983, 101:253-269.
17. deWet JR, Wood KV, DeLuca M, Helinski DR, Subramani S: Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol 1987, 7:725-737.
18. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC: Green fluorescent protein as a marker for gene expression. Science 1994, 263:802-805.
19. Vietes MJ, Navarro-Garcia R, Pla PJ, Nombela C: Expression and in vivo determination of fire fly luciferase as gene reporter in Saccharomyces cerevesiae. Yeast 1994, 10:1321-1327.
20. Thorvaldsen JL, Sewell AK, McCowen CL, Winge DR: Regulation of metallothionein genes by the ACEI and AMT1 transcription factors. J Biol Chem 1993, 268:12512-12518.
21. Smith JM, Tang CM, Noorden SV, Holden DW: Virulence of Aspergillus fumigatus double mutants lacking restrictocin and alkaline protease in a low-dose model of invasive pulmonary Aspergillosis. Infect Immun 1994, 62:5247-5254.
22. Woods JP, Heinecke EL, Goldman WE: Electrotransformation and expression of bacterial genes encoding hygromycin phosphotransferase and β-galactosidase in the pathogenic fungus Histoplasma capsulatum. Infect Immun 1998, 66:1697-1707.
23. Patel JP, Batanghari JW, Goldman WE: Probing the yeast phase-specific expression of the CBP1 gene in Histoplasma capsulatum. J Bacteriol 1998, 180:1786-1792.
24. Ohama T, Suzuki T, Mori M, Osawa S, Ueda T, Watanabe K, Nakase T: Non-universal decoding of the leucine codon CUG in several Candida species. Nucleic Acids Res 1993, 21:4039-4045.
25. Leuker CE, Ernst JF: Toxicity of a heterologous leucyl-tRNA (anticodon CAG) in the pathogen Candida albicans; in vivo evidence for non-standard decoding of CUG codons. Mol Gen Genet 1994, 245:212-217.
26. White C, Andrews E, Maltby D, Agabian N: The 'universal' leucine codon CTG in the secreted aspartyl proteinase 1 (SAP1) gene of Candida albicans encodes a serine in vivo. J Bacteriol 1995, 177:2953-2955.
27. Leuker CE, Hahn A, Ernst JF: β-Galactosidase of Kluyveromyces lactis (Lac4P) as reporter of gene expression in Candida albicans and C. tropicalis. Mol Gen Genet 1992, 235:235-241.
28. Cormack BP, Bertram G, Egerton M, Gow NA, Falkow S, Brown AJP: Yeast-enhanced green fluorescent protein (YEGFP) a reporter of gene expression in Candida albicans. Microbiology 1997, 143:303-311. This paper describes the development of a GFP variant with enhanced green fluorescence that is active in C. albicans. The reporter gene product can be monitored in single cells using flow cytometer. This study demonstrated for the first time that the alteration of a single CUG codon is not sufficient to obtain delectable fluorescence in vivo and that the normalization of codon pattern to that of highly expressed genes of C. albicans required to enhance fluorescence.
29. Morschäuser J, Michel S, Hacker J: Expression of a chromosomally integrated, single-copy GFP gene is Candida albicans, and its use as a reporter of gene regulation. Mol Gen Genet 1998, 257:412-420. This paper describes the development of a GFP variant that has the single CUG codon altered according to C. albicans usage. The study demonstrates that addition customization as described by Cormack et al. [28••] may not be necessary for expression.
30. Leuker CE, Sonnebonn A, Delbruck S, Ernst JF: Sequence and promoter regulation of the PCK1 gene encoding phosphoenolpyruvate carboxykinase of the fungal pathogen Candida albicans. Gene 1997, 192:235-240. In this study, the potential range of sensitivity of the Lac4p reporter for C. albicans was improved by using a fluorogenic substrate. Reporter activity could be used to measure both the repression and induction kinetics.
31. Lorenz WW, McCann RO, Longiaru M, Cormier MJ: Isolation and expression of a cDNA encoding Renilla reniformis luciferase. Proc Natl Acad Sci USA 1991, 88:4438-4442.
32. Mitra RD, Silva CM, Youvan DC: Fluorescene resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein. Gene 1996, 173:13-17.
33. Myers KK, Sypherd PS, Fonzi WA: Use of URA3 as a reporter of gene expression in C. albicans. Curr Genet 1995, 27:243-248.
34. Pla J, Gil C, Monteoliva L, Navarro-Garcia F, Sanchez M, Nombela C: Understanding Candida albicans at the molecular level. Yeast 1996, 12:1677-1702.
35. Spellig T, Bottin A, Kahmann K: Green fluorescent protein (GFP) as a new vital marker in the phytopathogenic fungus Ustilago maydis. Mol Gen Genet 1996, 252:503-509.
36. Bronstein I, Fortin J, Stanley PE, Stewart GS, Kricka LJ: Chemiluminescent and bioluminescent reporter gene assays. Anal Biochemistry 1994, 219:169-181.
37. Liu J, O'Kane DJ, Escher A: Secretion of functional Renilla reniformis luciferase by mammalian cells. Gene 1997, 203:141-148. This article describes a novel RLUC reporter engineered to be secreted into a culture medium.
38. Pazzagli M, Devine JH, Peterson DO, Baldwin TO: Use of bacterial and firefly luciferases as reporter genes in DEAE-dextran-mediated transfection of mammalian cells. Anal Biochemistry 1992, 204:315-323.
39. Wood KV: The origin of beetle luciferases. In Bioluminescence and Chemiluminescence: Current Status. Edited by Stanley P, Kricka L Chichester: John Wiley and Sons; 1991:11-13.
40. Thompson JF, Hays LS, Lloyd DB: Modulation of firefly luciferase stability and impact on studies of gene regulation. Gene 1991, 103:171-177.