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1
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0029807797
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Once and only once upon a time: Specifying and regulating origins of DNA replication in eukaryotic cells
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Diffley JFX. Once and only once upon a time: specifying and regulating origins of DNA replication in eukaryotic cells. Genes Dev. 10:1996;2819-2830.
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Genes Dev
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Diffley, J.F.X.1
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2
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0029810269
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Cell cycle control of DNA replication
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Stillman B. Cell cycle control of DNA replication. Science. 274:1996;1659-1664.
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(1996)
Science
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Stillman, B.1
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3
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0030913801
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Chromatin proteins involved in the initiation of DNA replication
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Rowles A, Blow J. Chromatin proteins involved in the initiation of DNA replication. Curr Opin Gen Dev. 7:1997;152-157.
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(1997)
Curr Opin Gen Dev
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Rowles, A.1
Blow, J.2
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4
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0030834245
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Functional equivalency and diversity of cis-acting elements among yeast replication origins
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of special interest. Domain-swapping experiments between ARS305 and ARS1 demonstrate the diverse nature of the essential B-domain motifs between different S. cerevisiae origins.
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Lin S, Kowalski D. Functional equivalency and diversity of cis-acting elements among yeast replication origins. of special interest Mol Cell Biol. 17:1997;5473-5484 Domain-swapping experiments between ARS305 and ARS1 demonstrate the diverse nature of the essential B-domain motifs between different S. cerevisiae origins.
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(1997)
Mol Cell Biol
, vol.17
, pp. 5473-5484
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Lin, S.1
Kowalski, D.2
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5
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0030849742
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Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs
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of outstanding interest. See annotation [6].
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Tanaka T, Knapp D, Nasmyth K. Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs. of outstanding interest Cell. 90:1997;649-660 See annotation [6].
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(1997)
Cell
, vol.90
, pp. 649-660
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Tanaka, T.1
Knapp, D.2
Nasmyth, K.3
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6
-
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0030886099
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Components and dynamics of DNA replication complexes in S. cerevisiae: Redistribution of MCM proteins and Cdc45p during S-phase
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1-phase. After S-phase begins, Mcm and Cdc45 dissociate from origins and appear to move with the replication fork in concert with DNA polymerase ε.
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1-phase. After S-phase begins, Mcm and Cdc45 dissociate from origins and appear to move with the replication fork in concert with DNA polymerase ε.
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(1997)
Cell
, vol.91
, pp. 1-20
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Aparicio, O.1
Weinstein, D.2
Bell, S.3
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7
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0031002795
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Coordinate binding of ATP and origin DNA regulates the ATPase activity of the origin recognition complex
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of special interest. Origin recognition by ORC requires ATP. Here it is shown that ATP binding and hydrolysis by ORC are regulated by origin DNA. ATP binding, but not hydrolysis, was required for an ORC-origin interaction. This suggests that ATP functions as a cofactor to lock ORC onto origin DNA whereas its hydrolysis may drive transitions between functional states of ORC.
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Klemm R, Austin R, Bell S. Coordinate binding of ATP and origin DNA regulates the ATPase activity of the origin recognition complex. of special interest Cell. 88:1997;493-502 Origin recognition by ORC requires ATP. Here it is shown that ATP binding and hydrolysis by ORC are regulated by origin DNA. ATP binding, but not hydrolysis, was required for an ORC-origin interaction. This suggests that ATP functions as a cofactor to lock ORC onto origin DNA whereas its hydrolysis may drive transitions between functional states of ORC.
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(1997)
Cell
, vol.88
, pp. 493-502
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Klemm, R.1
Austin, R.2
Bell, S.3
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8
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0032472223
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Discrete start sites for DNA synthesis in the yeast ARS1 origin
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of outstanding interest. Mapping the 5′ ends of RNA primed nascent DNA in the vicinity of ARS1 localized the site of initiation of replication to an 18bp site adjacent to the ORC-binding site. These results suggest that ORC functions like other well characterized initiator proteins to dictate the sites of initiation of replication through local helix distortion.
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Belinsky A-K, Gerbi SA. Discrete start sites for DNA synthesis in the yeast ARS1 origin. of outstanding interest Science. 279:1998;95-98 Mapping the 5′ ends of RNA primed nascent DNA in the vicinity of ARS1 localized the site of initiation of replication to an 18bp site adjacent to the ORC-binding site. These results suggest that ORC functions like other well characterized initiator proteins to dictate the sites of initiation of replication through local helix distortion.
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(1998)
Science
, vol.279
, pp. 95-98
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Belinsky A-K1
Gerbi, S.A.2
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9
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0003890119
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A. Kornberg, Baker T. New York: Freeman, WH and Company
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Kornberg A, Baker T. DNA Replication - Second edition. 1992;Freeman, WH and Company, New York.
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(1992)
DNA Replication - Second Edition
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10
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0030904468
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1 phase
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of special interest. Cell-cycle analysis of Cdc6 expression and function. Cdc6 is expressed primarily between mitosis and Start, apparently by post-transcriptional controls.
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1 phase. of special interest J Cell Sci. 110:1997;753-763 Cell-cycle analysis of Cdc6 expression and function. Cdc6 is expressed primarily between mitosis and Start, apparently by post-transcriptional controls.
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(1997)
J Cell Sci
, vol.110
, pp. 753-763
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Detweiler, C.1
Li, J.2
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11
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0031056052
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CDC45, a novel yeast gene that functions with the origins recognition complex and Mcm proteins in initiation of DNA replication
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of special interest. See annotation [12].
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Zou L, Mitchell J, Stillman B. CDC45, a novel yeast gene that functions with the origins recognition complex and Mcm proteins in initiation of DNA replication. of special interest Mol Cell Biol. 17:1997;553-563 See annotation [12].
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(1997)
Mol Cell Biol
, vol.17
, pp. 553-563
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-
Zou, L.1
Mitchell, J.2
Stillman, B.3
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12
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0030845932
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Characterization of Cdc47p-minichromosome maintenance complexes in Saccharomyces cerevisiae: Identification of Cdc45p as a subunit
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of special interest. Cdc45 interacts genetically with the Mcm genes and is further characterized in this report and [11]. Cdc45 is synthetically lethal with ORC, Mcm2 and Mcm3 [12]. It is a component of high molecular-weight MCM complexes in cell lysates, and remains nuclear throughout the cell cycle [12].
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Dalton S, Hopwood B. Characterization of Cdc47p-minichromosome maintenance complexes in Saccharomyces cerevisiae: identification of Cdc45p as a subunit. of special interest Mol Cell Biol. 17:1997;5869-5875 Cdc45 interacts genetically with the Mcm genes and is further characterized in this report and [11]. Cdc45 is synthetically lethal with ORC, Mcm2 and Mcm3 [12]. It is a component of high molecular-weight MCM complexes in cell lysates, and remains nuclear throughout the cell cycle [12].
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(1997)
Mol Cell Biol
, vol.17
, pp. 5869-5875
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-
Dalton, S.1
Hopwood, B.2
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13
-
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0030951331
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Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast
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of special interest. Mcm complexes are found associated with S. cerevisiae chromatin in a Cdc6-dependent fashion. Once bound, conditions were found to selectively wash ORC and Cdc6 from chromatin, without removing Mcm complexes. Thus, Cdc6 loads Mcm onto chromatin, after which Mcms interact with another tightly associated component of chromatin.
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Donovan S, Harwood J, Drury L, Diffley J. Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast. of special interest Proc Natl Acad Sci USA. 94:1997;5611-5616 Mcm complexes are found associated with S. cerevisiae chromatin in a Cdc6-dependent fashion. Once bound, conditions were found to selectively wash ORC and Cdc6 from chromatin, without removing Mcm complexes. Thus, Cdc6 loads Mcm onto chromatin, after which Mcms interact with another tightly associated component of chromatin.
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(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 5611-5616
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Donovan, S.1
Harwood, J.2
Drury, L.3
Diffley, J.4
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14
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0027978640
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Interaction of Dbf4, the Cdc7 protein kinase regulatory subunit, with yeast replication origins in vivo
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Dowell SJ, Romanowski P, Diffley JF. Interaction of Dbf4, the Cdc7 protein kinase regulatory subunit, with yeast replication origins in vivo. Science. 265:1994;1243-1246.
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(1994)
Science
, vol.265
, pp. 1243-1246
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Dowell, S.J.1
Romanowski, P.2
Diffley, J.F.3
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15
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0029906578
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Interaction between yeast Cdc6 protein and B-type cyclin/Cdc28 kinases
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Elasser S, Lou F, Wang B, Campbell J, Jong A. Interaction between yeast Cdc6 protein and B-type cyclin/Cdc28 kinases. Mol Biol Cell. 7:1996;1723-1735.
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Mol Biol Cell
, vol.7
, pp. 1723-1735
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Elasser, S.1
Lou, F.2
Wang, B.3
Campbell, J.4
Jong, A.5
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16
-
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0030924762
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Interaction of the S phase regulator cd18 with cyclin dependent kinase in fission yeast
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of special interest. A fusion protein of Cdc18, the S. pombe homologue of Cdc6, copurifies with an active B-cdk complex. This interaction suggests that the B-cdk complex may interact directly with the pre-RC.
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Brown G, Jallepalli P, Huneycutt B, Kelly T. Interaction of the S phase regulator cd18 with cyclin dependent kinase in fission yeast. of special interest Proc Natl Acad Sci USA. 94:1997;6142-6147 A fusion protein of Cdc18, the S. pombe homologue of Cdc6, copurifies with an active B-cdk complex. This interaction suggests that the B-cdk complex may interact directly with the pre-RC.
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(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 6142-6147
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Brown, G.1
Jallepalli, P.2
Huneycutt, B.3
Kelly, T.4
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17
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0031006539
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Mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p
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of special interest. Mutations in one of the members of the Mcm complex suppress mutations in Cdc7. This genetic interaction suggests that Cdc7 may interact directly with the pre-RC.
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Hardy C, Dryga O, Seematter S, Pahl P, Sclafani R. mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p. of special interest Proc Natl Acad Sci USA. 94:1997;3151-3155 Mutations in one of the members of the Mcm complex suppress mutations in Cdc7. This genetic interaction suggests that Cdc7 may interact directly with the pre-RC.
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(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 3151-3155
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Hardy, C.1
Dryga, O.2
Seematter, S.3
Pahl, P.4
Sclafani, R.5
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18
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0030974160
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A lesion in the DNA replication initiation factor Mcm10 induces pausing of elongation forks through chromosomal replication origins in Saccharomyces cerevisiae
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of outstanding interest. A novel gene product is shown to be essential for DNA replication in S. cerevisiae. Mcm10 mutants show defects in ARS plasmid maintenance and Mcm10 interacts physically with the Mcm complex members. Mcm10 mutants appear to cause replication forks to pause at origins, as if Mcm10 is required to disassemble pre-RCs.
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Merchant A, Dawasaki Y, Chen Y, Lei M, Tye B. A lesion in the DNA replication initiation factor Mcm10 induces pausing of elongation forks through chromosomal replication origins in Saccharomyces cerevisiae. of outstanding interest Mol Cell Biol. 17:1997;3261-3271 A novel gene product is shown to be essential for DNA replication in S. cerevisiae. Mcm10 mutants show defects in ARS plasmid maintenance and Mcm10 interacts physically with the Mcm complex members. Mcm10 mutants appear to cause replication forks to pause at origins, as if Mcm10 is required to disassemble pre-RCs.
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(1997)
Mol Cell Biol
, vol.17
, pp. 3261-3271
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Merchant, A.1
Dawasaki, Y.2
Chen, Y.3
Lei, M.4
Tye, B.5
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19
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0030859463
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A DNA helicase activity is associated with an Mcm4, -6, and -7 protein complex
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of outstanding interest. of special interest. The replicative helicase has yet to be identified. This paper reveals that both an ATPase activity and DNA helicase activity co-purifies with a complex of three Mcm proteins. Taken together with [6], this makes the Mcm complex an attractive candidate for a helicase at the replication fork.
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Ishimi Y. A DNA helicase activity is associated with an Mcm4, -6, and -7 protein complex. of outstanding interest. of special interest J Biol Chem. 272:1997;24508-24513 The replicative helicase has yet to be identified. This paper reveals that both an ATPase activity and DNA helicase activity co-purifies with a complex of three Mcm proteins. Taken together with [6], this makes the Mcm complex an attractive candidate for a helicase at the replication fork.
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(1997)
J Biol Chem
, vol.272
, pp. 24508-24513
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Ishimi, Y.1
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20
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0029761435
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1 defines a 'point of no return' after which Cdc6 synthesis cannot promote DNA replication in yeast
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1 defines a 'point of no return' after which Cdc6 synthesis cannot promote DNA replication in yeast. Genes Dev. 10:1996;1516-1531.
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(1996)
Genes Dev
, vol.10
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Piatti, S.1
Bohm, T.2
Cocker, J.3
Diffley, J.4
Nasmyth, K.5
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21
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0029797418
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Cell-cycle control of S phase: A comparison of two yeasts
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Huberman J. Cell-cycle control of S phase: a comparison of two yeasts. Chromosoma. 105:1996;197-203.
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Chromosoma
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Huberman, J.1
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0030906338
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Cyclin-dependent kinase and initiation at eukaryotic origin: A replication switch?
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jallepalli P, Kelly T. Cyclin-dependent kinase and initiation at eukaryotic origin: a replication switch? Curr Opin Cell Biol. 9:1997;358-363.
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Curr Opin Cell Biol
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Jallepalli, P.1
Kelly, T.2
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23
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0030920723
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ORC-dependent and origin-specific initiation of DNA replication at defined foci in isolated yeast nuclei
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1-phase nuclei - initiation of replication can be elicited with soluble S-phase promoting factors.
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1-phase nuclei - initiation of replication can be elicited with soluble S-phase promoting factors.
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(1997)
Genes Dev
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Pasero, P.1
Braguglia, D.2
Gasser, S.3
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25
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0029007149
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The Chinese hamster dihydrofolate reductase origin consists of multiple potential nascent-strand start sites
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Dijkwel PA, Hamlin JL. The Chinese hamster dihydrofolate reductase origin consists of multiple potential nascent-strand start sites. Mol Cell Biol. 15:1995;3023-3031.
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Mol Cell Biol
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Dijkwel, P.A.1
Hamlin, J.L.2
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26
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85081192648
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Identification of primary sites for DNA replication in the hamster DHFR gene initiation zone
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in press
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Kobayashi T, Rein T, DePamphilis ML. Identification of primary sites for DNA replication in the hamster DHFR gene initiation zone. of outstanding interest Mol Cell Biol. 1998; Extensive mapping of the 5′ ends of nascent DNA strands at the DHFR origin locus reveals a novel preferred initiation site (ori-β′) 5kb downstream of ori-β. Strict interpretation of 2D gel analyses [27] is still not compatible with two distinct initiation sites in this region but this represents the first time that a non-2D gel technique has corroborated the presence of additional initiation sites near ori-β and suggests that this technique could reveal other preferred initiation sites throughout the broad initiation zone defined by 2D gels. 2D gel analyses of DNA segments between ori-β and ori-β′ should help to clarify whether data from these two techniques can finally be reconciled.
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(1998)
Mol Cell Biol
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Kobayashi, T.1
Rein, T.2
Depamphilis, M.L.3
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27
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0029740659
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Characterizing replication intermediates in the amplified CHO dihydrofolate reductase domain by two novel gel electrophoretic techniques
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Kalejta R, Lin H-B, Dijkwel P, Hamlin J. Characterizing replication intermediates in the amplified CHO dihydrofolate reductase domain by two novel gel electrophoretic techniques. Mol Cell Biol. 16:1996;4923-4931.
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Mol Cell Biol
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Kalejta, R.1
Lin H-B2
Dijkwel, P.3
Hamlin, J.4
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28
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0028929362
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Mapping of replication initiation sites in human ribosomal DNA by nascent-strand abundance analysis
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Yoon Y, Sanchez A, Brun C, Huberman JA. Mapping of replication initiation sites in human ribosomal DNA by nascent-strand abundance analysis. Mol Cell Biol. 15:1995;2482-2489.
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Mol Cell Biol
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Yoon, Y.1
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Brun, C.3
Huberman, J.A.4
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29
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0029931399
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DNA replication initiates non-randomly at multiple sites near the c-myc gene in HeLa cells
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Waltz S, Trivedi A, Leffak M. DNA replication initiates non-randomly at multiple sites near the c-myc gene in HeLa cells. Nucleic Acids Res. 24:1996;1887-1894.
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Waltz, S.1
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Leffak, M.3
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30
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0027970415
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Mapping an initiation region of DNA replication at a single-copy chromosomal locus in Drosophila melanogaster cells by two-dimensional gel methods and PCR-mediated nascent-strand analysis: Multiple replication origins in a broad zone
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Shinomiya T, Ina S. Mapping an initiation region of DNA replication at a single-copy chromosomal locus in Drosophila melanogaster cells by two-dimensional gel methods and PCR-mediated nascent-strand analysis: multiple replication origins in a broad zone. Mol Cell Biol. 14:1994;7394-7403.
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Mol Cell Biol
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Shinomiya, T.1
Ina, S.2
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31
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0028971217
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Participation of the human β-globin locus control region in initiation of DNA replication
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Aladjem M, Groudine M, Brody L, Dieken E, Fournier R, Wahl G, Epner E. Participation of the human β-globin locus control region in initiation of DNA replication. Science. 270:1995;815-819.
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Science
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Aladjem, M.1
Groudine, M.2
Brody, L.3
Dieken, E.4
Fournier, R.5
Wahl, G.6
Epner, E.7
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32
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0029815014
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Utilization of the same DNA replication origin by human cells of different derivation
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Kumar S, Giacca M, Norio P, Biamonti G, Riva S, Falaschi A. Utilization of the same DNA replication origin by human cells of different derivation. Nucleic Acids Res. 24:1996;3289-3294.
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Nucleic Acids Res
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Kumar, S.1
Giacca, M.2
Norio, P.3
Biamonti, G.4
Riva, S.5
Falaschi, A.6
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33
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0030840416
-
Regulation of the replication of the murine immunoglobulin heavy chain gene locus: Evaluation of the role of the 3′ regulatory region
-
of special interest. Previous studies showed that a translocation dissociating the lgh genes from a putative LCR located 40kb downstream of Cα alters the replication pattern at this locus. In this report, a B-cell line with a 34kb deletion encompassing the LCR is examined. Although there is a marked reduction in expression of the lgh genes, no alteration in the lgh replication pattern was detected.
-
Michaelson J, Ermakova O, Birshtein B, Ashouian N, Chevillard C, Riblet R, Schildkraut C. Regulation of the replication of the murine immunoglobulin heavy chain gene locus: evaluation of the role of the 3′ regulatory region. of special interest Mol Cell Biol. 17:1997;6167-6174 Previous studies showed that a translocation dissociating the lgh genes from a putative LCR located 40kb downstream of Cα alters the replication pattern at this locus. In this report, a B-cell line with a 34kb deletion encompassing the LCR is examined. Although there is a marked reduction in expression of the lgh genes, no alteration in the lgh replication pattern was detected.
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(1997)
Mol Cell Biol
, vol.17
, pp. 6167-6174
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-
Michaelson, J.1
Ermakova, O.2
Birshtein, B.3
Ashouian, N.4
Chevillard, C.5
Riblet, R.6
Schildkraut, C.7
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34
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0027968172
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Densely methylated DNA islands in mammalian chromosomal replication origins
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Tasheva ES, Roufa DJ. Densely methylated DNA islands in mammalian chromosomal replication origins. Mol Cell Biol. 14:1994;5636-5644.
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(1994)
Mol Cell Biol
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Tasheva, E.S.1
Roufa, D.J.2
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35
-
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0030899373
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Absence of an unusual "densely methylated island" at the hamster dhfr ori-β
-
of special interest. See annotation [36].
-
Rein T, Natale D, Gartner U, Niggemann M, DePamphilis M, Zorbas H. Absence of an unusual "densely methylated island" at the hamster dhfr ori-β of special interest J Biol Chem. 272:1997;10021-10029 See annotation [36].
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(1997)
J Biol Chem
, vol.272
, pp. 10021-10029
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Rein, T.1
Natale, D.2
Gartner, U.3
Niggemann, M.4
Depamphilis, M.5
Zorbas, H.6
-
36
-
-
0031024587
-
mCpG dinucleotides
-
of special interest. This paper and [35] thoroughly re-analyze the methylation status of sequences near the CHO DHFR ori-β. In [35], the 'densely methylated island' reported in [34] was not identified and appears to be an artifact of the technique used in that study. In [36], the authors identify a separate but adjacent DNA segment where mCpGs were found at an unusually high frequency.
-
mCpG dinucleotides. of special interest Mol Cell Biol. 17:1997;416-426 This paper and [35] thoroughly re-analyze the methylation status of sequences near the CHO DHFR ori-β. In [35], the 'densely methylated island' reported in [34] was not identified and appears to be an artifact of the technique used in that study. In [36], the authors identify a separate but adjacent DNA segment where mCpGs were found at an unusually high frequency.
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(1997)
Mol Cell Biol
, vol.17
, pp. 416-426
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Rein, T.1
Zorbas, H.2
Depamphilis, M.L.3
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37
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0029007145
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Site-specific initiation of DNA replication in Xenopus egg extract requires nuclear structure
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Gilbert DM, Miyazawa H, DePamphilis ML. Site-specific initiation of DNA replication in Xenopus egg extract requires nuclear structure. Mol Cell Biol. 15:1995;2942-2954.
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(1995)
Mol Cell Biol
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Gilbert, D.M.1
Miyazawa, H.2
Depamphilis, M.L.3
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38
-
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0029891988
-
Both cyclin A and cyclin E have S-phase promoting SPF activity in Xenopus egg extracts
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Strausfeld U, Howell M, Descombes P, Chevalier S, Rempel R, Adamczewski J, Maller J, Hunt T, Blow J. Both cyclin A and cyclin E have S-phase promoting SPF activity in Xenopus egg extracts. J Cell Sci. 109:1996;1555-1563.
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J Cell Sci
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Strausfeld, U.1
Howell, M.2
Descombes, P.3
Chevalier, S.4
Rempel, R.5
AdaMcZewski, J.6
Maller, J.7
Hunt, T.8
Blow, J.9
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39
-
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0031022391
-
Cell cycle regulation of the replication licensing system: Involvement of a Cdk-dependent inhibitor
-
of special interest. Fractionation of Xenopus egg extracts separates replication licensing factor activity into two components, RLFM, which corresponds to the Mcm complex, and RLFB, a partially purified activity. Here, it is shown that there are at least two ways that RLF activity is regulated during the Xenopus embryonic cell-cycle. First, an RLF inhibitor, which appears to be Cdc2/CyclinB, prevents licensing activity during metaphase. Second, RLFB activity, which rises rapidly after metaphase, declines during S-phase in the absence of the RLF inhibitor.
-
Mahbubani H, Chong J, Chevalier S, Thommes P, Blow J. Cell cycle regulation of the replication licensing system: involvement of a Cdk-dependent inhibitor. of special interest J Cell Biol. 136:1997;125-135 Fractionation of Xenopus egg extracts separates replication licensing factor activity into two components, RLFM, which corresponds to the Mcm complex, and RLFB, a partially purified activity. Here, it is shown that there are at least two ways that RLF activity is regulated during the Xenopus embryonic cell-cycle. First, an RLF inhibitor, which appears to be Cdc2/CyclinB, prevents licensing activity during metaphase. Second, RLFB activity, which rises rapidly after metaphase, declines during S-phase in the absence of the RLF inhibitor.
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(1997)
J Cell Biol
, vol.136
, pp. 125-135
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Mahbubani, H.1
Chong, J.2
Chevalier, S.3
Thommes, P.4
Blow, J.5
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40
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0028906840
-
Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication
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Powers MA, Macaulay C, Masiarz FR, Forbes DJ. Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication. J Cell Biol. 128:1995;721-736.
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J Cell Biol
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Powers, M.A.1
MacAulay, C.2
Masiarz, F.R.3
Forbes, D.J.4
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41
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0027489289
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Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix
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Jenkins H, Holman T, Lyon C, Lane B, Stick R, Hutchinson C. Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix. J Cell Sci. 106:1993;275-285.
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(1993)
J Cell Sci
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Jenkins, H.1
Holman, T.2
Lyon, C.3
Lane, B.4
Stick, R.5
Hutchinson, C.6
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42
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0028972870
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Xenopus lamin B3 has a direct role in the assembly of a replication competent nucleus: Evidence from cell-free egg extracts
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Goldberg M, Jenkins H, Allen T, Whitfield W, Hutchison C. Xenopus lamin B3 has a direct role in the assembly of a replication competent nucleus: evidence from cell-free egg extracts. J Cell Sci. 108:1995;3451-3461.
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(1995)
J Cell Sci
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Goldberg, M.1
Jenkins, H.2
Allen, T.3
Whitfield, W.4
Hutchison, C.5
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43
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0030863126
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Disruption of nuclear lamin organization alters the distribution of replication factors and inhibits DNA synthesis
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of special interest. A truncated human lamin protein is used as a dominant negative mutant to perturb lamin organization. When added to Xenopus egg extracts, DNA replication was inhibited and the distributions of replication fork enzymes PCNA and RFC were dramatically altered within the nucleus. By contrast, the distribution of pre-RC proteins Mcm and ORC - as well as the initiation/replication fork enzyme DNA polymerase α - were not affected, suggesting an undefined specificity in the effects of the nuclear lamina on replication.
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Spann T, Moir R, Goldman A, Stick R, Goldman R. Disruption of nuclear lamin organization alters the distribution of replication factors and inhibits DNA synthesis. of special interest J Cell Biol. 136:1997;1201-1212 A truncated human lamin protein is used as a dominant negative mutant to perturb lamin organization. When added to Xenopus egg extracts, DNA replication was inhibited and the distributions of replication fork enzymes PCNA and RFC were dramatically altered within the nucleus. By contrast, the distribution of pre-RC proteins Mcm and ORC - as well as the initiation/replication fork enzyme DNA polymerase α - were not affected, suggesting an undefined specificity in the effects of the nuclear lamina on replication.
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(1997)
J Cell Biol
, vol.136
, pp. 1201-1212
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Spann, T.1
Moir, R.2
Goldman, A.3
Stick, R.4
Goldman, R.5
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44
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0023904730
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A role for the nuclear envelope in controlling DNA replication within the cell cycle
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Blow JJ, Laskey RA. A role for the nuclear envelope in controlling DNA replication within the cell cycle. Nature. 332:1988;546-548.
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(1988)
Nature
, vol.332
, pp. 546-548
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Blow, J.J.1
Laskey, R.A.2
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46
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0027177595
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Reversible effects of nuclear membrane permeabilization on DNA replication: Evidence for a positive licensing factor
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Coverley D, Downes CS, Romanowski P, Laskey RA. Reversible effects of nuclear membrane permeabilization on DNA replication: evidence for a positive licensing factor. J Cell Biol. 122:1993;985-992.
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(1993)
J Cell Biol
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Coverley, D.1
Downes, C.S.2
Romanowski, P.3
Laskey, R.A.4
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47
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0030904445
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A role for Cdk2 kinase in negatively regulating DNA replication during S phase of the cell-cycle
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of outstanding interest. It is shown that assembly of a nuclear envelope in Xenopus egg extract is followed by a 200-fold concentration of cdk2 kinase in the nucleus. By experimentally increasing the concentration of A- or E-cdk2 in extracts prior to the introduction of sperm chromatin, both replication and the binding of Mcm3 to chromatin were inhibited. These experiments suggest that the role of the nuclear envelope may be to concentrate cdk activity to levels that prevent re-initiation within one cell-cycle.
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Hua XH, Yan H, Newport J. A role for Cdk2 kinase in negatively regulating DNA replication during S phase of the cell-cycle. of outstanding interest J Cell Biol. 137:1997;183-192 It is shown that assembly of a nuclear envelope in Xenopus egg extract is followed by a 200-fold concentration of cdk2 kinase in the nucleus. By experimentally increasing the concentration of A- or E-cdk2 in extracts prior to the introduction of sperm chromatin, both replication and the binding of Mcm3 to chromatin were inhibited. These experiments suggest that the role of the nuclear envelope may be to concentrate cdk activity to levels that prevent re-initiation within one cell-cycle.
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(1997)
J Cell Biol
, vol.137
, pp. 183-192
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Hua, X.H.1
Yan, H.2
Newport, J.3
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48
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0028829358
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Transition in specification of embryonic metazoan DNA replication origins
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Hyrien O, Maric C, Mechali M. Transition in specification of embryonic metazoan DNA replication origins. Science. 270:1995;994-997.
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(1995)
Science
, vol.270
, pp. 994-997
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Hyrien, O.1
Maric, C.2
Mechali, M.3
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49
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0031037467
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Regulation of replicon size in Xenopus egg extracts
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of special interest. The concentration of sperm chromatin in Xenopus egg extracts was increased, resulting in a reduction in the rate of replication, presumably through an increase in replicon size. ORC was not the limiting factor, suggesting that some other factor controls how many ORC-DNA complexes initiate replication.
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Walter J, Newport J. Regulation of replicon size in Xenopus egg extracts. of special interest Science. 275:1997;993-995 The concentration of sperm chromatin in Xenopus egg extracts was increased, resulting in a reduction in the rate of replication, presumably through an increase in replicon size. ORC was not the limiting factor, suggesting that some other factor controls how many ORC-DNA complexes initiate replication.
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(1997)
Science
, vol.275
, pp. 993-995
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Walter, J.1
Newport, J.2
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50
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0030475416
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Chromosome architecture can dictate site-specific initiation of DNA replication in Xenopus egg extracts
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of outstanding interest. Post-ODP CHO nuclei were cycled through an in vitro Xenopus egg mitosis, resulting in the assembly of a Xenopus embryonic nuclear envelope around CHO post-ODP chromatin. Replication within these chimeric nuclei initiated at a novel specific site in the 5′ region of the DHFR structural gene. Inhibition of chromosome condensation during mitosis with either high concentrations of nuclei or a Topoisomerase II inhibitor prevented preferential initiation at this site. The same unusual site was recognized with CHO metaphase chromosomes as a substrate. Clearly, specification of origins in Xenopus egg extract is profoundly influenced by higher-order features of chromosome architecture.
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Lawlis SJ, Keezer SM, Wu J-R, Gilbert DM. Chromosome architecture can dictate site-specific initiation of DNA replication in Xenopus egg extracts. of outstanding interest J Cell Biol. 135:1996;1-12 Post-ODP CHO nuclei were cycled through an in vitro Xenopus egg mitosis, resulting in the assembly of a Xenopus embryonic nuclear envelope around CHO post-ODP chromatin. Replication within these chimeric nuclei initiated at a novel specific site in the 5′ region of the DHFR structural gene. Inhibition of chromosome condensation during mitosis with either high concentrations of nuclei or a Topoisomerase II inhibitor prevented preferential initiation at this site. The same unusual site was recognized with CHO metaphase chromosomes as a substrate. Clearly, specification of origins in Xenopus egg extract is profoundly influenced by higher-order features of chromosome architecture.
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(1996)
J Cell Biol
, vol.135
, pp. 1-12
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Lawlis, S.J.1
Keezer, S.M.2
Wu J-R3
Gilbert, D.M.4
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51
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0029670538
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1 step required to specify the Chinese hamster DHFR replication origin
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1 step required to specify the Chinese hamster DHFR replication origin. Science. 271:1996;1270-1272.
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(1996)
Science
, vol.271
, pp. 1270-1272
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Wu J-R1
Gilbert, D.M.2
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52
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0030835657
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1-phase step that precedes restriction point control
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1-phase, event that responds to internal cues and can be uncoupled from the signals that control licensing and cell proliferation.
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1-phase, event that responds to internal cues and can be uncoupled from the signals that control licensing and cell proliferation.
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(1997)
Mol Cell Biol
, vol.17
, pp. 4312-4321
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Wu J-R1
Gilbert, D.M.2
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53
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0031279834
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Origin-specific initiation of mammalian nuclear DNA replication in a Xenopus cell-free system
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Wu J-W, Yu G, Gilbert DM. Origin-specific initiation of mammalian nuclear DNA replication in a Xenopus cell-free system. Methods, A Companion to Methods In Enzymology. 13:1997;313-324.
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(1997)
Methods, a Companion to Methods in Enzymology
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, pp. 313-324
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Wu J-W1
Yu, G.2
Gilbert, D.M.3
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