메뉴 건너뛰기




Volumn 271, Issue 5253, 1996, Pages 1270-1272

A distinct G1 step required to specify the Chinese hamster DHFR replication origin

Author keywords

[No Author keywords available]

Indexed keywords

DIHYDROFOLATE REDUCTASE;

EID: 0029670538     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5253.1270     Document Type: Article
Times cited : (109)

References (19)
  • 2
    • 84920290813 scopus 로고
    • _, J. Biol. Chem 268, 1 (1993).
    • (1993) J. Biol. Chem , vol.268 , pp. 1
  • 6
    • 84920294961 scopus 로고    scopus 로고
    • note
    • Intact nuclei are defined by the exclusion of large fluorescently labeled molecules that lack a nuclear localization signal (Fig 1) Damaged nuclei are per-meable to such molecules
  • 8
  • 9
    • 84920294960 scopus 로고    scopus 로고
    • note
    • CHOC 400 is a CHO cell line that contains ∼1000 tandemly integrated copies of a 243-kb segment of DNA harboring the DHFR gene, the 2BE2121 gene, and a 55-kb intergenic region that were amplified by stepwise selection in methotrexate (4). The pattern of initiation of replication within the amplified locus is indistinguishable from that within the wild-type DHFR locus (70).
  • 11
    • 0029036901 scopus 로고    scopus 로고
    • M. A Madine, C.-Y. Khoo, A. D. Mills, R. A Laskey, Nature 375, 421 (1995); J. P. J. Chong, H. M Mahbuhani, C-Y. Khoo, J. J. Blow, ibid., p. 418; Y Kubota, S Mimura, S.-I. Nishimoto, H Takisawa, H Nojima, Cell 81, 601 (1995); M. A. Madine, C.-Y. Khoo, A. D. Mills, C. Musahl, R. A. Laskey, Curr. Biol. 5, 1270 (1995).
    • (1995) Nature , vol.375 , pp. 421
    • Madine, M.A.1    Khoo, C.-Y.2    Mills, A.D.3    Laskey, R.A.4
  • 12
    • 0029036901 scopus 로고    scopus 로고
    • M. A Madine, C.-Y. Khoo, A. D. Mills, R. A Laskey, Nature 375, 421 (1995); J. P. J. Chong, H. M Mahbuhani, C-Y. Khoo, J. J. Blow, ibid., p. 418; Y Kubota, S Mimura, S.-I. Nishimoto, H Takisawa, H Nojima, Cell 81, 601 (1995); M. A. Madine, C.-Y. Khoo, A. D. Mills, C. Musahl, R. A. Laskey, Curr. Biol. 5, 1270 (1995).
    • Nature , pp. 418
    • Chong, J.P.J.1    Mahbuhani, H.M.2    Khoo, C.-Y.3    Blow, J.J.4
  • 13
    • 0029000522 scopus 로고
    • M. A Madine, C.-Y. Khoo, A. D. Mills, R. A Laskey, Nature 375, 421 (1995); J. P. J. Chong, H. M Mahbuhani, C-Y. Khoo, J. J. Blow, ibid., p. 418; Y Kubota, S Mimura, S.-I. Nishimoto, H Takisawa, H Nojima, Cell 81, 601 (1995); M. A. Madine, C.-Y. Khoo, A. D. Mills, C. Musahl, R. A. Laskey, Curr. Biol. 5, 1270 (1995).
    • (1995) Cell , vol.81 , pp. 601
    • Kubota, Y.1    Mimura, S.2    Nishimoto, S.-I.3    Takisawa, H.4    Nojima, H.5
  • 14
    • 0029410628 scopus 로고
    • M. A Madine, C.-Y. Khoo, A. D. Mills, R. A Laskey, Nature 375, 421 (1995); J. P. J. Chong, H. M Mahbuhani, C-Y. Khoo, J. J. Blow, ibid., p. 418; Y Kubota, S Mimura, S.-I. Nishimoto, H Takisawa, H Nojima, Cell 81, 601 (1995); M. A. Madine, C.-Y. Khoo, A. D. Mills, C. Musahl, R. A. Laskey, Curr. Biol. 5, 1270 (1995).
    • (1995) Curr. Biol. , vol.5 , pp. 1270
    • Madine, M.A.1    Khoo, C.-Y.2    Mills, A.D.3    Musahl, C.4    Laskey, R.A.5
  • 15
    • 0026645239 scopus 로고
    • G. H. Leno et al. Cell 69, 151 (1992).
    • (1992) Cell , vol.69 , pp. 151
    • Leno, G.H.1
  • 17
    • 84920294958 scopus 로고    scopus 로고
    • note
    • 6 nuclei per milliliter were stained with DAPI (1 μg/ml) and affinity-purified, Texas red-labeled IgG (150 μg/ml) (Jackson Laboratory). Photographs were taken with a Nikon Labophot-2 fitted with a 100× Plan lens (numerical aperture, 1.25) and were composed with Photoshop software.
  • 18
    • 84920294957 scopus 로고    scopus 로고
    • note
    • 32P]deoxyadenosine 5′-triphosphate (25 μCl/ml). 6-DMAP was added to 3 mM. The percentage of labeled nuclei was determined by indirect immunofluorescence after incubation in extracts containing 250 μM bromodeoxyundine triphosphate. Similar results were obtained in four separate experiments, although the length of the lag period (10 to 30 min) and the total amount of DNA replicated varied with the batch of extract.
  • 19
    • 84920294956 scopus 로고    scopus 로고
    • note
    • We thank B. Knox, D. Dimitrova, M. DePamphilis, J. Huberman, and B. Burhans for critical reading of the manuscript and G. Wahl and G. Leno for helpful advice. Supported by grants to D M G. from the Hendricks Foundation, the Milheim Foundation, and the National Science Foundation (MCB-9505907).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.