Chromatin proteins involved in the initiation of DNA replication

Current Opinion in Genetics and Development

Volumn 7, Issue 2, 1997, Pages 152-157

  Rowles, Alison ^a   Blow, J Julian ^a  

^a IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CYCLE PROTEIN; PROTEIN; RIBOSOME RNA;

ARTICLE; CHROMATIN; DNA REPLICATION; EUKARYOTE; HUMAN; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; XENOPUS;

EID: 0030913801     PISSN: 0959437X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-437X(97)80123-2     Document Type: Article

References (57)

1. Bell SP, Stillman B: ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex Nature 1992, 357:128-134.
2. Bell S: Eukaryotic replicators and associated protein complexes. Curr Opin Genet Dev 1995, 5:162-167.
3. Diffley JF, Cocker JH: Protein-DNA interactions at a yeast replication origin. Nature 1992, 357:169-172.
4. Bell SP, Mitchell J, Leber J, Kobayashi R, Stillman B: The multidomain structure of Orc1p reveals similarity to regulators of DNA replication and transcriptional silencing. Cell 1995, 83:563-568. The cloning of ORC1, ORC3 and ORC4 is reported, completing the identification of S. cerevisiae ORC subunits. Reconstitution of ORC produced in insect cells gives the same DNase 1 footprint as that from ORC purified from yeast cells. Data is presented suggesting that the amino terminus of Orc1 is required for transcriptional silencing but is dispensable for DNA replication.
5. Gavin KA, Hidaka M, Stillman B: Conserved initiator proteins in eukaryotes. Science 1995, 270:1667-1871. Homologues of ORC1 in K. lactis, S. pombe and human cells and of ORC2 in A. thaliana, C. elegans and human cells were cloned. Genetic analysis demonstrated that S. pombe Orc1 is essential for cell viability. Human Orc1 was found complexed with Orc2 in whole-cell extracts.
6. Gossen M, Pak DTS, Hansen SK, Acharya JK, Botchan MR: A Drosophila homolog of the yeast origin recognition complex. Science 1995, 270:1674-1677. Homologues of ORC2 and ORC5 were cloned from Drosophila. Fractionation of embryo nuclear extracts showed that Orc2 and Orc5 form part of a complex containing four other polypeptides, having similar molecular weights to S. cerevisiae ORC subunits.
7. Carpenter PB, Mueller PR, Dunphy WG: Role for a Xenopus Orc2-related protein in controlling DNA replication. Nature 1996, 379:357-360. The authors of this paper report trie cloning of the Xenopus ORC2 gene, identified by its ability to rescue a mitotic catastrophe mutant of S. pombe. Immunodepletion of XOrc2 from Xenopus egg extracts abolishes the initiation of DNA replication. Immunofluorescence data suggests that XORC binds to chromatin before replication starts.
8. Leatherwood J, Lopezgirona A, Russell P: Interaction of cdc2 and cdc18 with a fission yeast orc2-like protein. Nature 1996, 379:360-363. The S. pombe orp2 gene was isolated in a two-hybrid screen for potential targets of the Cdc2 kinase. The Cdc2 protein interacts with Orp2 and Cdc18 in vitro. Genetic analysis indicates that orp2 has two functions in the cell cycle: it is required for DNA replication and is involved in generating a checkpoint signal to prevent aberrant mitoses.
9. Muzi Falconi M, Kelly TJ: Orp1, a member of the Cdc18/Cdc6 family of S-phase regulators, is homologous to a component of the origin recognition complex. Proc Natl Acad Sci USA 1995, 92:12475-12479. A search for homologues of the S. pombe cdc18 gene lead to the identification of orp1, a homologue of S. cerevisiae ORC1. orp1 is an essential gene the mRNA product of which is present throughout the cell cycle.
10. Takahara K, Bong M, Brevard R, Eddy RL, Haley LL, Sait SJ, Shows TB, Hoffman GG, Greenspan DS: Mouse and human homologues of the yeast origin of replication recognition complex subunit ORC2 and chromosomal localization of the cognate human gene ORC2L. Genomics 1996, 31:119-122.
11. Rowles A, Chong JPJ, Brown L, Howell M, Evan GI, Blow JJ: Interaction between the origin recognition complex and the replication licensing system in Xenopus. Cell 1996, 87:287-296. The cloning and analysis of the Xenopus ORC1 gene is reported in this paper. Immunodepletion of XOrc1 from Xenopus egg extract also removes XOrc2 and abolishes initiation of DNA replication. A Xenopus ORC complex (XORC) was purified, consisting of seven polypeptides with similar molecular weights to those of S. cerevisiae ORC. Although XORC has an activity distinct from licensing factor, it is required for the assembly of licensing factor components onto chromatin, suggesting a sequential assembly of these proteins onto replication origins.
12. 1/S boundary. Genetic and biochemical interactions were demonstrated with cdc21 (SpMCM4] and cdc18. orp1 is also involved in the checkpoint control that prevents entry into mitosis in the absence of DNA replication.
13. Donovan S, Diffley JFX: Replication origins in eukaryotes. Curr Opin Genet Dev 1996, 6:203-207.
14. Hardy C: Characterization of an essential Orc2p-associated factor that plays a role in DNA replication. Mol Cell Biol 1996, 16:1832-1841. This paper characterizes the phenotype of orc3 mutant S. cerevisiae cells but insists on calling the gene OAF1. ORC3 interacts with ORC2 in both a genetic and two-hybrid screen and provides evidence for an interaction between the two ORC subunits.
15. Chong JPJ, Thömmes P, Blow JJ: The role of MCM/P1 proteins in the licensing of DNA replication. Trends Biochem Sci 1996, 21:102-106.
16. Chong JPJ, Mahbubani MH, Khoo C-Y, Blow JJ: Purification of an Mcm-containing complex as a component of the DNA replication licensing system. Nature 1995, 375:418-421.
17. Maine GT, Sinha P, Tye BK: Mutants of S. cerevisiae defective in the maintenance of minichromosomes. Genetics 1984, 106:365-385.
18. Kimura H, Nozaki N, Sugimoto K: DNA polymerase alpha associated protein P1, a murine homolog of yeast MCM3, changes its intranuclear distribution during the DNA synthetic period. EMBO J 1994, 13:4311-4320.
19. Kubota Y, Mimura S, Nishimoto S, Takisawa H, Nojima H: Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor. Cell 1995, 81:601-609.
20. Madine MA, Khoo C-Y, Mills AD, Laskey RA: MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells. Nature 1995, 375:421-424.
21. Treisman JE, Follette PJ, O'Farrell PH, Rubin GM: Cell proliferation and DNA replication defects in a Drosophila mcm2 mutant. Genes Dev 1995, 9:1709-1715.
22. Todorov IT, Attaran A, Kearsey SE: BM28, a human member of the mcm2-3-5 family, is displaced from chromatin during DNA replication. J Cell Biol 1995, 129:1433-1445.
23. Madine MA, Khoo C-Y, Mills AD, Musahl C, Laskey RA: Nuclear envelope prevents re-replication by restricting binding of MCM3 to chromatin. Curr Biol 1995, 5:1270-1279.
24. Coué M, Kearsey SE, Méchali M: Chromatin binding, nuclear-localization and phosphorylation of Xenopus cdc21 are cell-cycle dependent and associated with the control of initiation of DNA replication. EMBO J 1996, 15:881-372. A report of the cloning ol a Xenopus homologue of cdc21 (XMCM4). Cdc21 binds to chromatin early in the cell cycle and is removed from the chromatin during S phase. Cdc21 is hyperphosphorylated during mitosis, is dephosphorylated on exit from metaphase, and is partially rephosphorylated when bound to chromatin.
25. Holthoff HP, Hameister H, Knippers R: A novel human Mcm protein - homology to the yeast replication protein Mis5 and chromosomal location. Genomics 1996, 37:131-134.
26. Burkhart R, Schulte D, Hu B, Musahl C, Gohring F, Knippers R: Interactions of human nuclear proteins P1mcm3 and P1cdc46. Eur J Biochem 1995, 228:431-438.
27. Kimura H, Takizawa N, Nozaki N, Sugimoto K: Molecular cloning of a cDNA encoding mouse Cdc21 and CDC46 homologs and characterization of the products: physical interaction between P1(MCM3) and CDC46 proteins. Nucleic Acids Res 1935, 23:2097-2104.
28. Musahl C, Schulte D, Burkhart R, Knippers R: A human homolog of the yeast replication protein Cdc21 - interactions with other Mcm-proteins. Eur J Biochem 1995, 230:1096-1101.
29. Ishimi Y, Ichinose S, Omori A, Sato K, Kimura H: Binding of human minichromosome maintenance proteins with histone H3. J Biol Chem 1996, 271:24115-24122. Human MCM/P1 proteins were partially purified from HeLa cell extracts. Six proteins, Mcm2-7 were detectable. Of these, Mcm2, -4, -6 and -7 bound to a histone sepharose column. The Mcm complex had highest affinity for histone H3. This paper provides the first evidence that MCM/P1 proteins associate with histones and suggests they may assemble onto chromatin by binding to nucleosomes.
30. Lei M, Kawasaki Y, Tye B: Physical interactions among Mcm proteins and effects of Mcm dosage on DNA replication in Saccharomyces cerevisiae. Mol Cell Biol 1996, 16:5081-5090. Two-hybrid technology, immunoprecipitation and Mcm2-affinity chromatography were used to demonstrate that Mcm2, -3 and -5 form complexes in S. cerevisiae. Mcm2 and Mcm3 are both abundant proteins. Reduction of cellular Mcm2 levels in hemizygous diploids diminished use of specific replication origins, suggesting that a significant molar excess is required.
31. Coleman TR, Carpenter PB, Dunphy WG: The Xenopus cdc6 protein is essential for the initiation of a single round of DNA-replication in cell-free extracts. Cell 1996, 87:53-63. A report of the cloning of a Xenopus homologue of S. cerevisiae CDC6. Immunodepletion of XCdc6 from Xenopus egg extracts abolishes replication initiation, XCdc6 binds to chromatin early in the cell cycle and redistributes during S phase. Analysis of nuclei assembled in extracts depleted of either XOrc2, XMcm3 or XCdc6 demonstrated a sequential assembly of these proteins onto chromatin: XCdc6 requires XOrc2 to bind, whereas XMcm3 requires both XCdc6 and XOrc2.
32. 2 nuclei are unable to replicate in XMcm3-depleted extracts but do replicate in XOrc2-depleted extracts, showing that although MCM/P1 proteins require XORC to bind chromatin they are functionally distinct activities.
33. Mahbubani H, Chong J, Chevalier S, Thömmes P, Blow J: Cell cycle regulation of the replication licensing system: involvement of a Cdk-dependent inhibitor. J Cell Biol 1997, 136:125-135. An investigation of the regulation of the two components of replication licensing factor: RLF-M and RLF-B. RLF-B activity is periodic, being inactive in metaphase, activated in anaphase and decaying during late interphase, whereas RLF-M activity remains constant throughout the cell cycle. Lack of RLF-B activity in metaphase is caused by the presence of a Cdk-dependent inhibitor. Addition of the protein kinase inhibitor 6-DMAP to egg extracts stabilizes both Cyclin B and the RLF-B inhibitor activity. These data suggest that Cdks play an important role in regulating RLF activity.
34. 1/S phase boundary, consistent with the protein having a role in the initiation of DNA replication.
35. 1/S phase boundary. Deletion of cdc18 prevents S phase entry and cells then undergo a lethal mitotic division. Overproduction of the protein results in cells rereplicating their DNA.
36. Piatti S, Lengauer C, Nasmyth K: Cdc6 is an unstable protein whose de-novo synthesis in G(1) is important for the onset of S-phase and for preventing a reductional anaphase in the budding yeast Saccharomyces cerevisiae. EMBO J 1995, 14:3788-3799.
37. Diffley JF, Cocker JH, Dowell SJ, Rowley A: Two steps in the assembly of complexes at yeast replication origins in vivo. Cell 1994, 78:303-316.
38. 1 of the cell cycle, the prereplicative complex is thermolabile in cdc6ts mutants.
39. Liang C, Weinreich M, Stillman B: ORC and Cdc6p interact and determine the frequency of initiation of DNA replication in the genome. Cell 1995, 81:667-676
40. + regulators of DNA replication [abstract]. J Invest Med 1996, 44:198.
41. Hayles J, Fisher D, Woollard A, Nurse P: Temporal order of S phase and mitosis in fission yeast is determined by the state of the p34cdc2-mitotic B cyclin complex. Cell 1994, 78:813-822.
42. Labib K, Craven RA, Crawford K, Nurse P: Dominant mutants identify new roles for p34(Cdc2) in mitosis. EMBO J 1995, 14:2155-2165.
43. + gene. Nature 1994, 367:236-242.
44. 2 cells results in the inhibition of Cdc2-Cdc13 kinase and leads to repeated rounds of DNA synthesis.
45. Jallepalli PV, Kelly TJ: Rum1 and Cdc18 link inhibition of cyclin-dependent kinase to the initiation of DNA replication In Schizosaccharomyces pombe. Genes Dev 1996, 10:541-552. The data presented here provide a link between the S. pombe mitotic Cdk inhibitor rum1 and cdc18, rum1 is a multicopy suppressor of the cdc18 gene, whereas deletion of rum1 increased the severity of a cdc18 mutant phenotype. The Rum1 protein was also shown to biochemically interact with Cdc2-Cyclin B and inhibit its associated kinase activity.
46. 1 phase until anaphase, providing a possible mechanism for inhibiting prereplicative complex formation during this period.
47. Hamlin JL, Dijkwel PA: On the nature of replication origins in higher eukaryotes. Curr Opin Genet Dev 1995, 5:153-161.
48. Hyrien O, Maric C, Méchali M: Transition in specification of embryonic metazoan DNA replication origins. Science 1985, 270:994-997. Early Xenopus embryos initiate DNA replication in a sequence-independent manner at 9-12kbp intervals. Later in development, replication initiation is repressed within transcribed regions whereas initiation remains high in intergenic DNA. This demonstrates a transition in origin usage during develop-ment.
49. Kitsberg D, Selig S, Keshet I, Cedar H: Replication structure of the human beta-globin gene domain. Nature 1993, 366:586-590.
50. Aladjem M, Groudine M, Brody L, Dieken E, Fournier R, Wahl G, Epner E: Participation of the Human β-globin locus control region in initiation of DNA replication. Science 1995, 270:915-819. DNA replication preferentially initiates from an origin within the human β globin locus, situated 50 kbp downstream from the LCR. In a naturally occurring deletion of the human β globin LCR, the normal origin of replication is no longer used and replication initiates from a region outside the locus. This suggests that mammalian origins may require DNA sequence elements distal to the site of initiation.
51. Little R, Platt T, Schildkraut C: Initiation and termination of DNA replication in human rRNA genes. Mol Cell Biol 1993, 13:6600-6613.
52. Yoon Y, Sanchez JA, Brun C, Huberman JA: Mapping of replication initiation sites in human ribosomal DNA by nascent-strand abundance analysis. Mol Cell Biol 1995, 15:2482-2489.
53. Bickmore WA, Oghene K: Visualizing the spatial relationships between defined DNA-sequences and the axial region of extracted metaphase chromosomes. Cell 1996, 84:95-104. Fluorescence in situ hybridization on human metaphase chromosomes demonstrated that non-transcribed spacer regions of rDNA are more closely associated to the chromosome axis than the transcribed regions. Previous studies have identified an origin of replication within this region, supporting the hypothesis that origins of replication associate with the chromosome scaffold.
54. Marsden M, Laemmli U: Metaphase chromosome structure: evidence for a radial loop model. Cell 1979, 17:349-358.
55. Buongiorno-Nardelli M, Micheli G, Carri M, Marilley M: A relationship between replicon size and supercoiled loop domains in the eukaryotic genome. Nature 1982, 298:100-102.
56. Gilbert DM, Miyazawa H, Depamphilis ML: Site-specific initiation of DNA replication in Xenopus egg extract requires nuclear structure. Mol Cell Biol 1995, 15:2942-2954.
57. 1 specifically initiate replication from on β, an origin normally used in vivo. This transition defines the origin decision point.