Hepatitis G virus encodes protease activities which can effect processing of the virus putative nonstructural proteins

Journal of Virology

Volumn 72, Issue 1, 1998, Pages 868-872

  Belyaev, Alexander S ^a   Chong, Susan ^a   Novikov, Alexander ^a   Kongpachith, Ana ^a   Masiarz, Frank R ^b,c   Lim, Moon ^a   Kim, Jungsuh P ^a  

^a Genelabs Incorporated   (United States)

^b NOVARTIS PHARMA AG   (Switzerland)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEINASE; STRUCTURAL PROTEIN; VIRUS PROTEIN;

ANIMAL CELL; ARTICLE; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME SPECIFICITY; GENETIC ANALYSIS; HEPATITIS C VIRUS; HEPATITIS G VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROCESSING; SEQUENCE HOMOLOGY;

EID: 0031975194     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.72.1.868-872.1998     Document Type: Article

References (34)

1. Bartenschlager, R., L. Ahlborn-Laake, J. Mous, and H. Jacobsen. 1993. Nonstructural protein 3 of the hepatitis C virus encodes a serine-type proteinase required for cleavage at the NS3/4 and NS4/5 junctions. J. Virol. 67:3835-3844.
2. Bartenschlager, R., L. Ahlborn-Laake, J. Mous, and H. Jacobsen. 1994. Kinetic and structural analyses of hepatitis C virus polyprotein processing. J. Virol. 68:5045-5055.
3. Bartenschlager, R., V. Lohmann, T. Wilkinson, and J. O. Koch. 1995. Complex formation between the NS3 serine-type proteinase of the hepatitis C virus and NS4A and its importance for polyprotein maturation. J. Virol. 69:7519-7528.
4. Chou, P. Y., and G. D. Fasman. 1978. Prediction of the secondary structure of proteins from their amino acid sequence. Adv. Enzymol. Relat. Areas Mol. Biol. 47:45-148.
5. Davies, A. H., J. B. M. Jowett, and I. M. Jones. 1993. Recombinant baculovirus vectors expressing glutathione-S-transferase fusion proteins. Bio/Technolouy 11:933-936.
6. Failla, C. M., E. Pizzi, R. De Francesco, and A. Tramontano. 1996. Redesigning the substrate specificity of the hepatitis C virus NS3 protease. Folding Design 1:35-42.
7. Grakoui, A., D. W. McCourt, C. Wychowski, S. M. Feinstone, and C. M. Rice. 1993. Characterization of the hepatitis C virus-encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites. J. Virol. 67:2832-2843.
8. Grakoui, A., D. W. McCourt, C. Wychowski, S. M. Feinstone, and C. M. Rice. 1993. A second hepatitis C virus-encoded proteinase. Proc. Natl. Acad. Sci. USA 90:10583-10587.
9. Hijikata, M., H. Mizushima, T. Akagi, S. Mori, N. Kakiuchi, N. Kato, T. Tanaka, K. Kimura, and K. Shimotohno. 1993. Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus. J. Virol. 67:4655-4675.
10. Kim, J. L., K. A. Morgenstern, C. Lin, T. Fox, M. D. Dwyer, J. A. Landro, S. P. Chambers, W. Markland, C. A. Lepre, E. T. O'Malley, S. L. Sharbeson, C. M. Rice, M. A. Murcko, P. R. Caron, and J. A. Thomson. 1996. Crystal structure of the hepatitis C virus NS3 protease domain complexed with a synthetic NS4A peptide. Cell 87:343-355.
11. King, L. A., and R. D. Possee. 1992. The baculovirus expression system: a laboratory guide. Chapman & Hall, London, United Kingdom.
12. Kitts, P. A., and R. D. Possee. 1993. A method for producing recombinant baculovirus expression vectors at high frequency. BioTechniques 14:810-817.
13. Koch, J. O., V. Lohmann, U. Herian, and R. Bartenschlager. 1996. In vitro studies on the activation of the hepatitis C virus NS3 proteinase by the NS4A cofactor. Virology 221:54-66.
14. Kolykhalov, A. A., E. V. Agapov, and C. M. Rice. 1994. Specificity of the hepatitis C virus serine protease: effects of substitutions at the 3/4A, 4A/4B, 4B/5A, and 5A/5B cleavage sites on polyprotein processing. J. Virol. 68:7525-7533.
15. Kunkel, T. A. 1985. Rapid and efficient site-directed mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
16. Lin, C., B. M. Prágai, A. Grakoui, J. Xu, and C. M. Rice. 1994. The hepatitis C virus NS3 serine proteinase: trans-cleavage requirements and processing kinetics. J. Virol. 68:8147-8157.
17. Lin, C., J. A. Thomson, and C. M. Rice. 1995. A central region in the hepatitis C virus NS4A protein allows formation of an active NS3-NS4A serine protease complex in vivo and in vitro. J. Virol. 69:4373-4380.
18. Linnen, J., J. Wages, Z.-Y. Zhang-Keck, K. Fry, K. Z. Krawczynski, H. Alter, E. Koonin, M. Gallagher, M. Alter, S. Hadziyannis, P. Karayiannis, K. Fung, Y. Nakatsuji, J. Shih, L. Young, M. Piatak, C. Hoover, J. Fernandez, S. Chen, J. C. Zou, T. Morris, K. C. Hyams, S. Ismay, J. D. Lifson, G. Hess, S. K. H. Foung, H. Thomas, D. Bradley, H. Margolis, and J. P. Kim. 1996. Molecular cloning and disease association of hepatitis G virus: a new transfusion transmissible agent. Science 271:505-508.
19. Linnen, J., et al. Personal communication.
20. Love, R. A., H. E. Parge, J. A. Wickersham, Z. Hostomsky, N. Habuka, E. W. Moomaw, T. Adachi, and Z. Hostomska. 1996. The crystal structure of hepatitis C virus NS3 proteinase reveals a trypsin-like fold and a structural zinc binding site. Cell 87:331-342.
21. Manabe, S., I. Fuke, O. Tanishita, C. Kaji, Y. Gomi, S. Yoshida, C. Mori, A. Takamizawa, I. Yoshida, and H. Okayama. 1994. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-644.
22. Mori, A., K. Yamada, J. Kimura, T. Koide, S. Yausa, E. Yamada, and T. Miyamura. 1996. Enzymatic characterization of purified NS3 serine proteinase of hepatitis C virus expressed in Escherichia coli. FEBS Lett. 378:37-42.
23. Neddermann, P., L. Tomei, C. Steinkuhler, P. Gallinari, A. Tramontane, and R. De Francesco. 1997. The nonstructural proteins of the hepatitis C virus: structure and functions. Biol. Chem. 378:469-476.
24. Pizzi, E., A. Tramontano, L. Tomei, N. La Monica, C. Failla, M. Sardana, T. Wood, and R. de Francesco. 1994. Molecular model of the specificity pocket of the hepatitis C virus protease: implication for substrate recognition. Proc. Natl. Acad. Sci. USA 91:888-892.
25. Reed, K. E., A. Grakoui, and C. M. Rice. 1995. Hepatitis C virus-encoded NS2-3 protease: cleavage site mutagenesis and requirements for bimolecular cleavage. J. Gen. Virol. 69:4127-4136.
26. Scarselli, E., A. Urbani, A. Sbardellati, L. Tomei, R. De Francesco, and C. Traboni. 1997. GB virus B and hepatitis C virus NS3 serine proteases share substrate specificity. J. Virol. 71:4985-4989.
27. Schlauder, G. G., G. Dawson, J. N. Simons, T. J. Pilot-Matias, R. A. Gutierrez, C. A. Heynen, M. F. Knigge, G. S. Kurpiewski, S. L. Buijk, T. P. Leary, A. S. Muerhoff, S. M. Desai, and I. K. Mushahwar. 1995. Molecular and serologic analysis in the transmission of the GB hepatitis agents. J. Med. Virol. 46:81-90.
28. Shimizu, Y., K. Yamaji, Y. Masuho, T. Yokota, H. Inoue, K. Sudo, S. Satoh, and K. Shimotohno. 1996. Identification of the sequence on NS4A required for enhanced cleavage of the NS5A/5B site by hepatitis C virus NS3 protease. J. Virol. 70:127-132.
29. Simons, J. N., T. P. Leary, G. J. Dawson, T. J. Pilot-Matias, A. S. Muerhoff, G. Schlauder, S. M. Desai, and I. K. Mushahwar. 1995. Isolation of novel virus-like sequences associated with human hepatitis. Nat. Med. 1:564-569.
30. Tanji, Y., M. Hijikata, S. Satoh, T. Kaneko, and K. Shimotohno. 1995. Hepatitis C virus-encoded nonstructural protein NS4A has versatile functions in viral protein processing. J. Virol. 69:1575-1581.
31. Tomei, L., C. Failla, E. Santolini, R. De Francesco, and N. La Monica. 1993. NS3 is a serine protease required for processing of hepatitis C virus polyprotein. J. Virol. 67:4017-4026.
32. Urbani, A., E. Bianchi, F. Narjes, A. Tramontano, R. De Francesco, C. Steinkuhler, and A. Pessi. 1997. Substrate specificity of the hepatitis C virus serine protease NS3. J. Biol. Chem. 272:9204-9209.
33. Zhang, R., J. Durkin, W. T. Windsor, C. McNemar, L. Ramanathan, and H. V. Le. 1997. Probing the substrate specificity of hepatitis C virus NS3 serine protease by using synthetic peptides. J. Virol. 71:6208-6213.
34. Zuckerman, A. J. 1996. Alphabet of hepatitis viruses. Lancet 347:558-559.