Identification of the sequence on NS4A required for enhanced cleavage of the NS5A/5B site by hepatitis C virus NS3 protease

Journal of Virology

Volumn 70, Issue 1, 1996, Pages 127-132

  Shimizu, Yasuaki ^a   Yamaji, Kayo ^a   Masuho, Yasuhiko ^a   Yokota, Tomoyuki ^b   Inoue, Hiroshi ^b   Sudo, Kenji ^b   Satoh, Shinya ^c   Shimotohno, Kunitada ^c  

^a ASTELLAS PHARMA INC   (Japan)

^b Rational Drug Design Laboratories   (Japan)

^c NATIONAL CANCER CENTER RESEARCH INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEINASE;

ARTICLE; ENZYME ACTIVITY; GENE SEQUENCE; HEPATITIS C VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; VIRUS GENE; VIRUS REPLICATION;

AMINO ACID SEQUENCE; BINDING SITES; HUMAN; MOLECULAR SEQUENCE DATA; PEPTIDES; SERINE ENDOPEPTIDASES; SUPPORT, NON-U.S. GOV'T; VIRAL NONSTRUCTURAL PROTEINS;

EID: 0029655995     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.1.127-132.1996     Document Type: Article

References (38)

1. Atherton, E., L. R. Cameron, and R. C. Sheppard. 1988. Peptide synthesis. 10. Use of pentafluorophenyl esters of fluorenyl-methoxycarbonylamino acids in solid phase synthesis Tetrahedron 44:843-857.
2. Bartenschlager, R. L., L. Ahlborn-Laake, J. Mous, and H. Jacobsen. 1994. Kinetic and structural analysis of hepatitis C virus polyprotein processing. J. Virol 68:5045-5055.
3. Bazan, J. F., and R. J. Fletterick. 1989 Detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses. Virology 171:637-639.
4. Bidlingmeyer, B. A., S. A. Cohen, and T. L. Tarrin. 1984. Rapid analysis of amino acids using pre-column derivatization. Biochemistry 336:93-104
5. Chambers, T. J., C. S. Hahn, R. Galler, and C. M. Rice. 1990 Flavivirus genome organization, expression, and replication. Annu. Rev Microbiol. 44:649-6887
6. Chambers, T. J., R. C. Weir, A. Grakoui, D. W. McCoort, J. F. Bazan, R. J. Fletterick, and C. M. Rice. 1991. Processing of the yellow fever virus nonstructural polyprotein: a catalytically active NS3 proteinase domain and NS2B are required for cleavages at dibasic sites. J. Virol. 65:6042-6050
7. Choo, Q.-L., K. H. Richman, J. H. Ran, K. Berger, C. Lee, C. Dong, C. Gallegos, D. Coit, A. Medina-Selby, P. J. Barr, A. J. Weiner, D. W. Bradley, G. Kuo, and M. Houghton. 1991. Genomic organization and diversity of the hepatitis, C virus. Proc. Natl. Acad. Sci. USA 88:2451-2455.
8. Collett, M. S. 1992. Molecular genetics of pestiviruses. Comp. Immunol. Microbiol. infect. Dis. 15:145-154.
9. Failla, C., L. Tomei, and R. De Francesco. 1994. Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins. J. Virol. 68:3753-3760.
10. Falgout, B., R. H. Miller, and C.-J. Lai. 1993. Deletion analysis of dengue virus type 4 nonstructural protein NS2B: identification of a domain required for NS2B-NS3 protease activity. J. Virol. 67:2034-2042.
11. Falgout, B., M. Pethel, Y.-M. Zhang, and C.-J. Lai. 1991. Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins. J Virol 65:2467-2475.
12. Francki, R. I. B., C. M. Fauquet, D. L. Knudson, and F. Brown. 1991. Classification and nomenclature of viruses: fifth report of the International Committee on Taxonomy of Viruses. Arch. Virol. 1991(Suppl. 2):223-233
13. Grakoui, A., D. W. McCourt, C. Wychowski, S. M. Feinstone, and C. M. Rice. 1993. Characterization of the hepatitis C virus-encoded serine proteinase: determination of protease-dependent polyprotein cleavage sites J. Virol 67:2832-2843
14. Grakoui, A., D. W. McCourt, C. Wychowski, S. M. Feinstone, and C. M. Rice. 1993. A second hepatitis C virus-encoded proteinase. Proc. Natl. Acad. Sci. USA 90:10583-10587
15. Grakoui, A., C. Wychowski, C. Lin, S. M. Feinstone, and C. M. Rice. 1993. Expression and identification of hepatitis C virus polyprotein cleavage products. J. Virol. 67:1385-1395.
16. Hahm, B., D. S. Han, S. H. Back, O.-K. Song, M.-J. Cho, C.-J. Kim, K. Shimotohno, and S. K. Jang. 1995. NS3-4A of hepatitis C virus is a chymotrypsin-like protease. J. Virol. 69:2534-2539
17. Hijikata, M., H. Mizushima, T. Akagi, S. Mori, N. Kakinchi, N. Kato, T. Tanaka, K. Kimura, and K. Shimotohno. 1993. Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus. J. Virol. 67:4665-4675
18. Hijikata, M., H. Mizushima, Y. Tanji, Y. Komada, Y. Hirowatari, T. Akagi, N. Kato, K. Kimura, and K. Shimotohno. 1993. Proteolytic processing and membrane association of putative nonstructural proteins of hepatitis C virus. Proc. Natl. Acad Sci. USA 90:10773-10777.
19. Houghton, M., A. Weiner, J. Han, G. Kuo, and Q.-L. Choo. 1991 Molecular biology of the hepatitis C virus: implications for diagnosis, development and control of viral disease Hepatology 14:381-388
20. Kakiuchi, N., M. Hijikata, Y. Komoda, Y. Tanji, Y. Hirowatari, and K. Shimotohno. 1995. Bacterial expression and analysis of cleavage activity of HCV serine proteinase using recombinant and synthetic substrate. Biochem Biophys. Res. Commun. 210:1059-1065.
21. Kaneko, T., Y. Tanji, S. Satoh, M. Hijikata, S. Asabe, K. Kimura, and K. Shimotohno. 1994. Production of two phosphoproteins from the NS5A region of the hepatitis C viral genome. Biochem Biophys. Res. Commun 205:320-326.
22. Kato, N., M. Hijikata, M. Nakagawa, Y. Ootsuyama, K. Muraiso, S. Ohkoshi, and K. Shimotohno. 1991. Molecular structure of the Japanese hepatitis C viral genome. FEBS Lett 280:325-328.
23. Kato, N., M. Hijikata, Y. Ootsuyama, M. Nakagawa, S. Ohkoshi, T. Sugimura, and K. Shimotohno. 1990. Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proc. Natl. Acad. Sci. USA 87:9524-9528.
24. Lin, C., B. D. Lindenbach, B. M. Pragai, D. W. McCourt, and C. M. Rice. 1994. Processing of the hepatitis C virus E2-NS2 region: identification of p7 and two distinct E2-specific products with different C termini. J. Virol. 68:5063-5073.
25. Lin, C., B. M. Prágai, A. Grakoui, J. Xu, and C. M. Rice. 1994. Hepatitis C virus NS3 serine protease: trans-cleavage requirements and processing kinetics J Virol 68:8147-8157.
26. Lin, C., J. A. Thomson, and C. M. Rice. 1995. A central region in the hepatitis C virus NS4A protein allows formation of an active NS3-NS4A serine proteinase complex in vivo and in vitro. J. Virol. 69:4373-4380.
27. Matsuura, Y., and T. Miyamura. 1993. The molecular biology of hepatitis C virus. Semin. Virol. 4:297-304.
28. Mizushima, H., H. Hijikata, S.-I. Asabe, M. Hirota, K. Kimura, and K. Shimotohno. 1994. Two hepatitis C virus glycoprotein E2 products with different C termini. J. Virol. 68:6215-6222.
29. Pizzi, E., A. Tramontano L. Tomei, N. La Monica, C. Failla, M. Sardana, T. Wood, and R. De Francesco. 1994. Molecular model of the specificity pocket of the hepatitis C virus protease: implications for substrate recognition Proc. Natl. Acad. Sci. USA 91:888-892.
30. Rawlings, N. D., and A. J. Barrett. 1994. Families of serine peptidases Methods Enzymol. 244:19-61.
31. Satoh, S., Y. Tanji, M. Hijikata, K. Kimura, and K. Shimotohno. 1995. The N-terminal region of hepatitis C virus nonstructural protein 3 (NS3) is essential for stable complex formation with NS4A. J. Virol. 69:4255-4260.
32. Selby, M. J., Q.-L. Choo, K. Berger, G. Kuo, E. Glazer, M. Eckart, C. Lee, D. Chien, C. Kuo, and M. Houghton. 1993. Expression, identification, and subcellular localization of the proteins encoded by the hepatitis C virus genome J Gen. Virol 74:1103-1113.
33. Takamizawa, A., C. Mori, I. Fuke, S. Manabe, J. Murakami, E. Fujita, E. Onishi, I. Andoh, I. Yoshida, and H. Okayama. 1991. Structure and organization of the hepatitis C virus genome isolated from human carriers. J. Virol. 65:1105-1113.
34. Tanaka, T., N. Kato, M. Nakagawa, Y. Ootsuyama, M.-J. Cho, T. Nakazawa, M. Hijikata, Y. Ishimura, and K. Shimotohno. 1992. Molecular cloning of hepatitis C virus genome from a single Japanese carrier, sequence variation within the same individual and among infected individuals. Virus Res. 23: 39-53.
35. Tanji, Y., M. Hijikata, S. Satoh, T. Kaneko, and K. Shimotohno. 1995. Hepatitis C virus-encoded nonstructural protein NS4A has versatile functions in viral protein processing. J. Virol 69:1575-1581.
36. Tanji, Y., K. Takashi, S. Satoh, and K. Shimotohno. 1995. Phosphorylation of hepatitis C virus-encoded nonstructural protein NS5A J. Virol. 69:3980-3986.
37. Tomei, L., C. Failla, E. Santolini, R. De Francesco, and N. La Monica. 1993. NS3 is a serine protease required for processing of hepatitis C virus polyprotein. J Virol. 67:4017-4026.
38. Wiskerchen, M., and M. S. Collett. 1991. Pestivirus gene expression: protein p80 of bovine viral diarrhea virus is a proteinase involved in polyprotein processing. Virology 184:341-350