Clinical-scale human umbilical cord blood cell expansion in a novel automated perfusion culture system

Bone Marrow Transplantation

Volumn 21, Issue 7, 1998, Pages 653-663

  Koller, M R ^a   Manchel, I ^a   Maher, R J ^a   Goltry, K L ^a   Armstrong, R D ^a   Smith, A K ^a  

^a Aastrom Biosciences Inc   (United States)

Author keywords

Bioreactor; Cell expansion; Hematopoiesis; Serum free medium; Stem cells; Umbilical cord blood

Indexed keywords

ERYTHROPOIETIN; GRANULOCYTE COLONY STIMULATING FACTOR; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; GROWTH FACTOR; INTERLEUKIN 1; INTERLEUKIN 11; INTERLEUKIN 3; INTERLEUKIN 6; LEUKOCYTE MIGRATION INHIBITION FACTOR; MACROPHAGE INFLAMMATORY PROTEIN 1ALPHA; MESSENGER RNA; TRANSFORMING GROWTH FACTOR BETA; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; BIOREACTOR; BLOOD CELL; CELL COUNT; CELL CULTURE; CELL DENSITY; COLONY FORMING UNIT GM; CONTROLLED STUDY; CRYOPRESERVATION; CULTURE MEDIUM; HEALTH CARE AVAILABILITY; HEMATOPOIESIS; HUMAN; HUMAN CELL; MESSENGER RNA SYNTHESIS; PERFUSION; PRIORITY JOURNAL; REPRODUCIBILITY; SAFETY; STEM CELL; STEM CELL TRANSPLANTATION; UMBILICAL CORD BLOOD;

EID: 0031922637     PISSN: 02683369     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bmt.1701157     Document Type: Article

References (58)

1. Gluckman E, Broxmeyer HE, Auerbach AD et al. Hematopoietic reconstitution in a patient with Fanconi's anemia by means of umbilical-cord blood from an HLA-identical sibling. New Engl J Med 1989; 321: 1174-1178.
2. Wagner JE, Kernan NA, Steinbuch M et al. Allogeneic sibling umbilical cord blood transplantation in children with malignant and non-malignant disease. Lancet 1995; 346: 214-219.
3. Kurtzberg J, Laughlin M, Graham ML et al. Placental blood as a source of hematopoietic stem cells for transplantation into unrelated recipients. New Engl J Med 1996; 335: 157-166.
4. Rubinstein P, Rosenfield RE, Adamson JW et al. Stored placental blood for unrelated bone marrow reconstitution, Blood 1993; 81: 1679-1690.
5. Rubinstein P, Carrier C, Adamson JW et al. New York Blood Center's program for unrelated placental/umbilical cord blood (PCB) transplantation: 243 transplants in the first 3 years. Blood 1996; 88: 142a (Abstr.).
6. Newton I, Charbord P, Schaal JP et al. Toward cord blood banking: density separation and cryopreservation of cord blood progenitors. Exp Hematol 1993; 21: 671-674.
7. Fritsch G, Stimpfl M, Buchinger P et al. Does cord blood contain enough progenitor cells for transplantation? J Hematother 1994; 3: 291-298.
8. Koller MR, Bender JG, Miller WM et al. Reduced oxygen tension increases hematopoiesis in long-term culture of human stem and progenitor cells from cord blood and bone marrow. Exp Hematol 1992; 20: 264-270.
9. Lu L, Xiao M, Clapp DW et al. High efficiency retroviralmediated gene transduction into single isolated immature and replatable CD34(3+) hematopoietic stem/progenitor cells from human umbilical cord blood. J Exp Med 1993; 178: 2089-2096.
10. Lansdorp PM, Dragowska W, Mayani H. Ontogeny-related changes in proliferative potential of human hematopoietic cells. J Exp Med 1993; 178: 787-791.
11. Hirao A, Kawano Y, Takaue Y et al. Engraftment potential of peripheral and cord blood stem cells evaluated by a long-term culture system. Exp Hematol 1994; 22: 521-526.
12. Traycoff CM, Kosak ST, Grigsby S et al. Evaluation of ex vivo expansion potential of cord blood and bone marrow hematopoietic progenitor cells using cell tracking and limiting dilution analysis. Blood 1995; 85: 2059-2068.
13. Vaziri H, Dragowska W, Allsopp RC et al. Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age. Proc Natl Acad Sci USA 1994; 91: 9857-9860.
14. - cells in cord blood and bone marrow. Blood 1995; 86: 3745-3753.
15. Vormoor J, Lapidot T, Pflumio F et al. Immature human cord blood progenitors engraft and proliferate to high levels in severe combined immunodeficient mice. Blood 1994; 83: 2489-2497.
16. Koller MR, Manchel I, Newsom BS et al. Bioreactor expansion of human bone marrow: comparison of unprocessed, density-separated and CD34-enriched cells. J Hematother 1995; 4: 159-169.
17. Koller MR, Palsson MA, Manchel I et al. LTC-IC expansion is dependent on frequent medium exchange combined with stromal and other accessory cell effects. Blood 1995; 86: 1784-1793.
18. Zandstra PW, Eaves CJ, Piret JM. Expansion of hematopoietic progenitor cell populations in stirred suspension bioreactors of normal human bone marrow cells. Biotechnol 1994; 12: 909-914.
19. + cell selection and perfusion on ex vivo expansion of peripheral blood mononuclear cells. Blood 1995; 86: 958-970.
20. Koller MR, Manchel I, Palsson MA et al. Different measures of human hematopoietic cell culture performance are optimized under vastly different conditions. Biotechnol Bioeng 1996; 50: 505-513.
21. + selection and T cell immunodepletion on cord blood hematopoietic progenitors: relevance to stem cell transphntation. Exp Hematol 1995; 23: 1492-1496.
22. Palsson BØ, Paek S-H, Schwanz RM et al. Expansion of human bone marrow progenitor cells in a high cell density continuous perfusion system. Bio/Technology 1993; 11: 368-371.
23. Koller MR, Emerson SG, Palsson BØ. Large-scale expansion of human stem and progenitor cells from bone marrow mononuclear cells in continuous perfusion culture. Blood 1993; 82: 378-384.
24. Silver SM, Adams PT, Hutchinson RJ et al. Phase I evaluation of ex vivo expanded hematopoietic cells produced by perfusion cultures in autologous bone marrow transplantation. Blood 1993; 82: 296a (Abstr.).
25. Champlin R, Mehra R, Gajewski J et al. Ex vivo expanded progenitor cell transplantation in patients with breast cancer. Blood 1995; 86: 295a (Abstr.).
26. Stiff PJ, Oldenberg D, Hsi E et al. Successful hematopoietic engraftment following high-dose chemotherapy using only ex vivo expanded bone marrow grown in Aastrom (stromal-based) bioreactors. Proc Am Soc Clin Oncol 1997; 16: 88a (Abstr.).
27. Koller MR, Oxender M, Brott DA et al. flt-3 ligand is more potent than c-kit ligand for the synergistic stimulation of ex vivo hematopoietic cell expansion. J Hematother 1996; 5: 449-459.
28. Yurasov SV, Flasshove M, Moore MAS. Density enrichment and characterization of hematopoietic progenitors and stem cells from umbilical cord blood. Bone Marrow Transplant 1996; 17: 517-525.
29. Schwartz RM, Palsson BØ, Emerson SG. Rapid medium perfusion rate significantly increases the productivity and longevity of human bone marrow cultures. Proc Natl Acad Sci USA 1991; 88: 6760-6764.
30. Armstrong RD, Koller MR, Maluta J et al. Clinical systems for the production of cells and tissues for human therapy. In: Oxender D, Post L (eds). Handbook of Experimental Pharmacology. Springer Verlag: Berlin, 1998 (in press).
31. Sutherland HJ, Lansdorp PM, Henkelman DH et al. Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow stromal layers. Proc Natl Acad Sci USA 1990; 87: 3584-3588.
32. de St. Groth SF. The evaluation of limiting dilution assays. J Immunol Meth 1982; 49: R11-R23.
33. Finney DJ. The estimation of bacterial densities from dilution series. J Hygiene 1951; 49: 26-35.
34. Guba SC, Sartor CI, Gottschalk LR et al. Bone marrow stromal fibroblasts secrete interleukin-6 and granulocyte-macrophage colony-stimulating factor in the absence of inflammatory stimulation: demonstration by serum-free bioassay, enzyme-linked immunosorbent assay, and reverse transcriptase polymerase chain reaction. Blood 1992; 80: 1190-1198.
35. Rozen S, Skaletsky HJ, Primer3. Code available at http://www-genome.wi.mit.edu/genomesoftware/other/primer 3.html. 1996.
36. Cluitmans FHM, Esendam BHJ, Landegent JE et al. Constitutive in vivo cytokine and hematopoietic growth factor expression in the bone marrow and peripheral blood of healthy individuals. Blood J 1995; 85: 2038-2044.
37. Aye MT, Hashemi S, Leclair B et al. Expression of stem cell factor and c-kit mRNA in cultured endothelial cells, monocytes and cloned human bone marrow stromal cells (CFU-RF). Exp Hematol 1992; 20: 523-527.
38. Lisovsky M, Braun SE, Ge Y et al. Flt3-ligand production by human bone marrow stromal cells. Leukemia 1996; 10: 1012-1018.
39. + blood dendritic cells. Blood 1995; 86: 3295-3301.
40. Kaashoek JGJ, Mout R, Falkenburg JHF et al. Cytokine production by the bladder carcinoma cell line 5637: rapid analysis of mRNA expression levels using a cDNA-PCR procedure. Lymph Cyto Res 1991; 10: 231-235.
41. + hematopoietic progenitor cells from patients with myelofibrosis and myeloid metaplasia. Blood 1996; 88: 4534-4546.
42. Koller MR, Maher RJ, Brott DA. LTC-IC assays performed in the presence of accessory cells are non-linear and semiquantitative. Blood 1996; 88: 457a (Abstr.).
43. Koller MR, Bradley MS, Palsson BØ. Growth factor consumption and production in perfusion cultures of human bone marrow correlates with specific cell production. Exp Hematol 1995; 23: 1275-1283.
44. Ramenghi U, Ruggieri L, Dianzani I et al. Human peripheral blood granulocytes and myeloid leukemic cell lines express both transcripts encoding for stem cell factor. Stem Cells 1994; 12: 521-526.
45. Watari K, Lansdorp PM, Dragowska W et al. Expression of interleukin-1 beta gene in candidate human hematopoietic stem cells. Blood 1994; 84: 36-43.
46. Lee SM, Knoppel E, van de Ven C et al. Transcriptional rates of granulocyte-macrophage colony-stimulating factor, granulocyte colony-stimulating factor, interleukin-3, and macrophage colony-stimulating factor genes in activated cord vs adult mononuclear cells: alteration in cytokine expression may be secondary to post-transcriptional instability. Pediatr Res 1993; 34: 560-564.
47. Chang M, Suen Y, Lee SM et al. Transforming growth factor-β1, macrophage inflammatory protein-1α, and interleukin-8 gene expression is lower in stimulated human neonatal compared with adult mononuclear cells. Blood 1994; 84: 118-124.
48. + hematopoietic stem cells. Blood 1994; 83: 3758-3779.
49. Hanania EG, Giles RE, Kavanigh J et al. Results of MDR-1 vector modification trial indicate that granulocyte-macrophage colony-forming unit cells do not contribute to post-transplant hematopoietic recovery following intensive systemic therapy. Proc Natl Acad Sci USA 1996; 93: 15346-15351.
50. Ploemacher RE, van der Sluijs JP, van Beurden CAJ et al. Use of limiting dilution type long-term marrow cultures in frequency analysis of marrow-repopulating and spleen colony-forming hematopoietic stem cells in the mouse. Blood 1991; 78: 2527-2533.
51. Kiefer F, Wagner EF, Keller G. Fractionation of mouse bone marrow by adherence separates primitive hematopoietic cells from in vitro colony-forming cells and spleen colony-forming cells. Blood 1991; 78: 2577-2582.
52. Szilvassy SJ, Weller KP, Chen B et al. Partially differentiated ex vivo expanded cells accelerate hematologic recovery in myeloablated mice transplanted with highly enriched long-term repopulating stem cells. Blood 1996; 88: 3642-3653.
53. Robertson MJ, Soiffer RJ, Freedman AS et al. Human bone marrow depleted of CD33-positive cells mediates delayed but durable reconstitution of hematopoiesis: clinical trial of MY9 monoclonal antibody-purged autografts for the treatment of acute myeloid leukemia. Blood 1992; 79: 2229-2236.
54. DiGiusto DL, Lee R, Moon J et al. Hematopoietic potential of cryopreserved and ex vivo manipulated umbilical cord blood progenitor cells evaluated in vitro and in vivo. Blood 1996; 87: 1261-1271.
55. Muench MO, Firpo MT, Moore MAS. Bone marrow transplantation with interleukin-1 plus kit-ligand ex vivo expanded bone marrow accelerates hematopoietic reconstitution in mice without loss of stem cell lineage and proliferative potential. Blood 1993; 81: 3463-3473.
56. Serrano F, Varas F, Bernad A et al. Accelerated and long-term hematopoietic engraftment in mice transplanted with ex vivo expanded bone marrow. Bone Marrow Transplant 1994; 14: 855-862.
57. Kohn DB, Weinberg KI, Nolta JA et al. Engraftment of genemodified umbilical cord blood cells in neonates with adenosine deaminase deficiency. Nature Med 1995; 1: 1017-1023.
58. Koller MR, Manchel I, Brott DA et al. Donor-to-donor variability in the expansion potential of human bone marrow cells is reduced by accessory cells but not by soluble growth factors. Exp Hematol 1996; 24: 1484-1493.