Different measures of human hematopoietic cell culture performance are optimized under vastly different conditions

Biotechnology and Bioengineering

Volumn 50, Issue 5, 1996, Pages 505-513

  Koller, Manfred R ^a   Manchel, Ilana ^a   Palsson, Mahshid A ^a   Maher, Robert J ^a   Palsson, Bernhard Ø ^a  

^a Aastrom Biosciences Inc   (United States)

Author keywords

bone marrow; culture optimization; hematopoiesis; perfusion; stem cells; stroma

Indexed keywords

BLOOD; CYTOLOGY; PHYSIOLOGY; TISSUE;

BONE MARROW; COLONY FORMING UNIT GRANULOCYTE MACROPHAGE CELLS; CULTURE OPTIMIZATION; HEMATOPOIESIS; LONG TERM CULTURE INITIATING CELL; PERFUSION; PREFORMED STROMA; STEM CELLS;

ANIMAL CELL CULTURE;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL PROLIFERATION; CELL SUBPOPULATION; COLONY FORMING UNIT GM; HEMATOPOIESIS; HEMATOPOIETIC CELL; HUMAN; HUMAN CELL; STEM CELL; TISSUE CULTURE;

EID: 0030570265     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19960605)50:5<505::AID-BIT4>3.0.CO;2-J     Document Type: Article

References (19)

1. Bentley, S. A. 1981. Close range cell: cell interaction required for stem cell maintenance in continuous bone marrow culture. Exp. Hematol. 9: 308-312.
2. Caldwell, J., Palsson, B. Ø., Locey, B., Emerson, S. G. 1991. Culture perfusion schedules influence the metabolic activity and granulocyte-macrophage colony-stimulating factor production rates of human bone marrow stromal cells. J. Cell. Physiol. 147: 344-353.
3. Dexter, T. M., Allen, T. D., Lajtha, L. G. 1977. Conditions controlling the proliferation of haemopoietic stem cells in vitro. J Cell Physiol. 91: 335-344.
4. Dorshkind, K. 1990. Regulation of hemopoiesis by bone marrow stromal cells and their products. Ann. Rev. Immunol. 8: 111-137.
5. Edgington, S M. 1992. New horizons for stem-cell bioreactors. Bio/Technology 10: 1099-1106.
6. Edgington, S. M. 1992. 3-D biotech: Tissue engineering. Bio/Technology 10: 855-860.
7. Gartner, S., Kaplan, H. S. 1980. Long-term culture of human bone marrow cells. Proc. Natl. Acad. Sci. 77: 4756-4759.
8. + cells into the myeloid lineage. Blood 80: 1405-1412.
9. --selected human peripheral blood progenitor cells. Blood 84: 2898-2903.
10. Koller, M. R., Bender, J. G., Miller, W. M., Papoutsakis, E. T. 1993. Expansion of human hematopoietic progenitors in a perfusion bioreactor system with IL-3, IL-6, and stem cell factor. Bio/Technology 11: 358-363.
11. - celts from patients is lower and more stromal-dependent than from normal donors. Blood 84: 495a (abstract).
12. Koller, M. R., Palsson, B. Ø. 1993. Tissue engineering: Reconstitution of human hematopoiesis ex vivo. Biotechnol. Bioeng. 42: 909-930.
13. Koller, M. R., Palsson, M. A., Manchel, I., Palsson, B. Ø. 1995. LTC-IC expansion is dependent on frequent medium exchange combined with stromal and other accessory cells. Blood 86:1784-1793.
14. Langer, R., Vacanti, J. P. 1993. Tissue engineering. Science 260: 920-926.
15. + cells in serum-free culture initiated with highly purified progenitor cells from human bone marrow. J. Exp. Med. 175: 1501-1509.
16. Oh, D.-J., Koller, M. R., Palsson, B. Ø. 1994. Frequent harvesting from perfused human bone marrow cultures results in increased cumulative cell and CFU-GM expansion. Biotechnol. Bioeng. 44: 609-616.
17. Palsson, B. Ø, Oh, D.-J., Koller, M. R. 1995. Replating of bioreactor expanded human bone marrow results in extended growth of primitive and mature cells. Cytotechnol. 18: 125-131.
18. Vaziri, H., Dragowska, W., Allsopp, R. C., Thomas, T. E., Harley, C. B., Lansdorp, P. M. 1994. Evidence for a mitotic clock in human hematopoietic stem cells: Loss of telomeric DNA with age. Proc. Natl. Acad. Sci. 91: 9857-9860.
19. Verfaillie, C. M. 1992. Direct contact between human primitive hematopoietic progenitors and bone marrow stroma is not required for long-term in vitro hematopoiesis. Blood 79: 2821-2826.