Interaction of CED-4 with CED-3 and CED-9: A molecular framework for cell death

Science

Volumn 275, Issue 5303, 1997, Pages 1122-1126

  Chinnaiyan, Arul M ^a   O'Rourke, Karen ^a   Lane, Brian R ^a   Dixit, Vishva M ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE PROTEINASE; INTERLEUKIN 1BETA CONVERTING ENZYME; PROTEIN BCL 2;

ARTICLE; CAENORHABDITIS ELEGANS; CELL DEATH; CELL PROTECTION; MOLECULAR INTERACTION; NEMATODE; NONHUMAN; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION;

ANIMALS; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; BCL-X PROTEIN; CAENORHABDITIS ELEGANS; CAENORHABDITIS ELEGANS PROTEINS; CALCIUM-BINDING PROTEINS; CASPASE 1; CASPASE 8; CASPASE 9; CASPASES; CELL LINE; CYSTEINE ENDOPEPTIDASES; GENES, HELMINTH; HELMINTH PROTEINS; HUMANS; MUTATION; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-BCL-2; TRANSFECTION; TUMOR CELLS, CULTURED;

CAENORHABDITIS ELEGANS; MAMMALIA;

EID: 0031034997     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.275.5303.1122     Document Type: Article

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30. Cell death assays were done as previously described (26-28). Transfection of 293T and MCF7 cells was done by calcium phosphate precipitation and lipofectamlne, respectively.
31. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
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34. Supported by the Medical Scientist Training Program (A.M.C. and B.R.L.) and NIH grant 07863 (A.M.C. and B.R.L). We thank H. R. Horvitz for the ced-4, ced-4L, and ced-3 clones, M. O. Hengartner for the ced-9 clone and sharing unpublished data, E. Koonin for suggesting mutations in CED-4, G. Chinnadurai for the HA-BAX, HA-BAK, and HA-BIK expression constructs, and E. H.-Y.Cheng and J. M. Hardwick for the Bcl-xL constructs. For technical help, advice, and reagents, we also thank the following members of the V.M.D. laboratory: I. Jones (preparation of figures), Y. Kuang, H. Duan, M. Muzio, J. Pan, J. McCarthy, E. Humke, T. Brtva, and C. Vincenz.