Mutations to alter Aspergillus awamori glucoamylase selectivity. III. Asn20→Cys/Ala27→Cys, Ala27→Pro, Ser30→Pro, Lys108→Arg, Lys108→Met, Gly137→Ala, 311-314 Loop, Tyr312→Trp and Ser436→Pro

Protein Engineering

Volumn 11, Issue 5, 1998, Pages 389-398

  Liu, Hsuan Liang ^a   Coutinho, Pedro M ^a   Ford, Clark ^b   Reilly, Peter J ^a  

^a 494 Town Engineering Building   (United States)

^b IOWA STATE UNIVERSITY   (United States)

Author keywords

Condensation; Glucoamylase; Glucose; Isomaltose; Site directed mutagenesis; Yield

Indexed keywords

GLUCAN 1,4 ALPHA GLUCOSIDASE; GLUCOSE; MALTODEXTRIN; MUTANT PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; ASPERGILLUS AWAMORI; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; GLUCONEOGENESIS; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL;

ASPERGILLUS AWAMORI;

EID: 0031841902     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/11.5.389     Document Type: Article

References (68)

1. Adachi, S., Ueda, Y. and Hashimoto, K. (1984) Biotechnol. Bioengng, 26, 121-127.
2. Aleshin, A., Golubev, A., Firsov, L.M. and Honzatko, R.B. (1992) J. Biol. Chem., 267, 19291-19298.
3. Aleshin, A.E., Firsov, L.M. and Honzatko, R.B. (1994) J. Biol. Chem., 269, 15631-15639.
4. Allen, M.J. (1997) Ph.D. Dissertation, Iowa State University, Ames, IA.
5. Bakir, U., Coutinho, P.M., Sullivan, P.A., Ford, C. and Reilly, P.J. (1993) Protein Engng, 6, 939-946.
6. Beschkov, V., Marc, A. and Engasser, J.M. (1984) Biotechnol. Bioengng, 26, 22-26.
7. Chen, H.-M., Bakir, U., Ford, C. and Reilly, P.J. (1994a) Biotechnol. Bioengng, 43, 101-105.
8. Chen, H.M., Ford, C. and Reilly, P. J. (1994b) Biochem. J., 301, 275-281.
9. Chen, H.-M., Ford, C. and Reilly, P.J. (1995) Protein Engng, 8, 575-582.
10. Chen, H.-M., Li, Y., Panda, T., Buehler, F.U., Ford, C. and Reilly, P.J. (1996) Protein Engng, 9, 499-505.
11. Christensen, U., Olsen, K., Stoffer, B.B. and Svensson, B. (1996) Biochemistry, 35, 15009-15018.
12. Coutinho, P.M. (1996) Ph.D. Dissertation, Iowa State University, Ames, IA.
13. Coutinho, P.M. and Reilly, P.J. (1994a) Protein Engng, 7, 393-400.
14. Coutinho, P.M. and Reilly, P.J. (1994b) Protein Engng, 7, 749-760.
15. Coutinho, P.M. and Reilly, P.J. (1997) Proteins, 29, 334-347.
16. Coutinho, P.M., Dowd, M.K. and Reilly, P.J. (1997a) Carbohydr. Res., 297, 309-324.
17. Coutinho, P.M., Dowd, M.K. and Reilly, P.J. (1997b) Proteins, 28, 162-173.
18. Coutinho, P. M., Dowd, M. K. and Reilly, P. J. (1998) Ind. Engng Chem. Res., in press.
19. Crabb, W.D. and Mitchinson, C. (1997) TIBTECH, 15, 349-352.
20. Davies, G.J., Wilson, K.S. and Henrissat, B. (1997) Biochem. J., 321, 557-559.
21. Fang, T.-Y., Coutinho, P.M., Reilly, P.J. and Ford, C. (1998a) Protein Engng, 11, 119-126.
22. Fang, T.-Y., Honzatko, R.B., Reilly, P.J. and Ford, C. (1998b) Protein Engng, 11, 127-133.
23. Fagerström, R. (1991) J. Gen. Microbiol., 137, 1001-1008.
24. Fagerström, R. and Kalkkinen, N. (1995) Biotechnol. Appl. Biochem., 21, 223-231.
25. Fersht, A.R. et al. (1985) Nature, 314, 235-238.
26. Fierobe, H.-P., Stoffer, B.B., Frandsen, T.P. and Svensson, B. (1996) Biochemistry, 35, 8696-8704.
27. Frandsen, T.P., Dupont, C., Lehmbeck, J., Stoffer, B., Sierks, M.R., Honzatko, R.B. and Svensson, B. (1994) Biochemistry, 33, 13808-13816.
28. Frandsen, T.P., Christensen, T., Stoffer, B., Lehmbeck, J., Dupont, C., Honzatko, R.B. and Svensson, B. (1995) Biochemistry, 34, 10162-10169.
29. Frandsen, T.P., Stoffer, B., Palcic, M.M., Hof, S. and Svensson, B. (1996) J. Mol. Biol., 263, 79-89.
30. Harris, E.M.S., Aleshin, A.E., Firsov, L.M. and Honzatko, R.B. (1993) Biochemistry, 32, 1618-1626.
31. Hehre, E.J., Okada, G. and Genghof, D.S. (1969) Arch. Biochem. Biophys., 135, 74-89.
32. Hiromi, K., Takahashi, K. Hamauzu, Z. and Ono, S. (1966a) J. Biochem., 59, 469-475.
33. Hiromi, K., Kawai, M. and Ono, S. (1966b) J. Biochem., 59, 476-480.
34. Hiromi, K., Nitta, Y. Numata, C. and Ono, S. (1973) Biochim. Biophys. Acta, 302, 362-375.
35. Itoh, T., Ohtsuki, I., Yamashita, I. and Fukui, S. (1987) J. Bacteriol., 169, 4171-4176.
36. Kitahata, S., Brewer, C.F., Genghof, D.S., Sawai, T. and Hehre, E.J. (1981) J. Biol. Chem., 256, 6017-6026.
37. Kunkel, T.A., Roberts, J.D. and Zakour, R.A. (1987) Methods Enzymol., 154, 367-382.
38. Lee, D.D., Lee, Y.Y., Reilly, P.J., Collins, E.V.Jr and Tsao, G.T. (1976) Biotechnol. Bioengng, 18, 253-267.
39. Li, Y. (1996) Ph.D. Dissertation, Iowa State University, Ames. IA.
40. Li, Y., Reilly, P.J. and Ford, C. (1997) Protein Engng, 10, 1199-1204.
41. Matsumura, M., Yasumura, S. and Aiba, S. (1986) Nature, 323, 356-358.
42. Meagher, M.M. and Reilly, P.J. (1989) Biotechnol. Bioengng, 34, 689-693.
43. Meagher, M.M., Nikolov, Z.L. and Reilly, P.J. (1989) Biotechnol. Bioengng, 34, 681-688.
44. Natarajan, S. and Sierks, M.R. (1996) Biochemistry, 35, 3050-3058.
45. Nikolov, Z.L., Meagher, M.M. and Reilly, P.J. (1989) Biotechnol. Bioengng, 34, 694-704.
46. Nunberg, J.H. et al. (1984) Mol. Cell Biol., 4, 2306-2315.
47. Ohnishi, M. (1990) Starch/Stärke, 42, 311-313.
48. Ohnishi, H., Kitamura, K., Minowa, T., Sakai, H. and Ohta, T. (1992) Eur. J. Biochem., 207, 413-418.
49. Olsen, K., Christensen, U., Sierks, M.R. and Svensson, B. (1993) Biochemistry, 32, 9686-9693.
50. Pazur, J.H. and Okada, S. (1967) Carbohydr. Res., 4, 371-379.
51. Pazur, J.H., Cepure, A., Okada, S. and Forsberg, L.S. (1977) Carbohydr. Res., 58, 193-202.
52. Rabbo, E. and Terkildsen, T.C. (1960) Scand. J. Lab. Invest., 12, 402-407.
53. Roels, J.A. and van Tilburg, R. (1979) Starch/Stärke, 31, 338-344.
54. Shiraishi, F., Kawakami, K. and Kusunoki, K. (1985) Biotechnol. Bioengng, 27, 498-502.
55. Sierks, M.R. and Svensson, B. (1992) Biochemistry, 32, 1113-1117.
56. Sierks, M.R. and Svensson, B. (1994) Protein Engng, 7, 1479-1484.
57. Sierks, M.R. and Svensson, B. (1996) Biochemistry, 35, 1865-1871.
58. Sierks, M.R., Ford, C., Reilly, P.J. and Svensson, B. (1989) Protein Engng, 2, 621-625.
59. Sierks, M.R., Ford, C., Reilly, P.J. and Svensson, B. (1990) Protein Engng, 3, 193-198
60. Sierks, M.R., Ford, C., Reilly, P.J. and Svensson, B. (1993) Protein Engng, 6, 75-79.
61. Sierks, M.R., Sico, C. and Zaw, M. (1997) Biotechnol. Prog., 13, 601-608.
62. Stoffer, B.B., Dupont, C., Frandsen, T.F., Lehmbeck, J. and Svensson, B. (1997) Protein Engng, 10, 81-87.
63. Svensson, B., Larsen, K., Svendsen, I. and Boel, E. (1983) Carlsberg Res. Commun., 48, 529-544.
64. Svensson, B., Frandsen, T.P., Matsui, I., Juge, N., Fierobe, H.-P., Stoffer, B. and Rodenburg, K.W. (1995) In Petersen, S.B., Svensson, B. and Pedersen, S. (eds), Carbohydrate Bioengineering. Elsevier, Amsterdam, pp. 125-145.
65. Tanaka, A. and Takeda, S. (1994) Biosci. Biotechnol. Bioengng, 58, 1809-1813.
66. Tanaka, Y., Ashikari, T., Nakamura, N., Kiuchi, N., Shibano, Y., Amachi, T. and Yoshizumi, H. (1986) Agric. Biol. Chem., 50, 965-969.
67. Wells, J.A. (1990) Biochemistry, 29, 8509-8517.
68. Wilkinson, A.J., Fersht, A.R., Blow, D.M. and Winter, G. (1983) Biochemistry, 22, 3581-3586.