Glucoamylase mutants in the conserved active-site segment Trp170-Tyr175 located at a distance from the site of catalysis

Protein Engineering

Volumn 10, Issue 1, 1997, Pages 81-87

  Stoffer, Bjarne B ^a,b   Dupont, Claude ^a,c   Frandsen, Torben P ^a   Lehmbeck, Jan ^d   Svensson, Birte ^a  

^a CARLSBERG LABORATORY   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c INRS INSTITUT ARMAND FRAPPIER   (Canada)

^d NOVO NORDISK A S   (Denmark)

Author keywords

3D model; Active site mutation; Mutational N deglycosylation; Subsite map; Thermostability

Indexed keywords

GLUCAN 1,4 ALPHA GLUCOSIDASE; ISOMALTOSE; MALTOTRIOSE; TRYPTOPHAN;

ARTICLE; ASPERGILLUS AWAMORI; ASPERGILLUS NIGER; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STABILITY; MUTAGENESIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; RHIZOPUS; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

ASPERGILLUS AWAMORI; ASPERGILLUS NIGER; FELIS CATUS; RHIZOPUS; RHIZOPUS ORYZAE;

EID: 0031025935     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/10.1.81     Document Type: Article

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