In vitro rejoining of DNA double strand breaks: A comparison of genomic- DNA with plasmid-DNA-based assays

International Journal of Radiation Biology

Volumn 73, Issue 5, 1998, Pages 481-493

  Cheong, N ^a   Perrault, A R ^a   Iliakis, G ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; PLASMID DNA;

ARTICLE; CANCER CELL CULTURE; DNA CONFORMATION; DNA REPAIR; DNA STRAND BREAKAGE; ENZYME ACTIVITY; HUMAN; HUMAN CELL; LUNG CANCER; PRIORITY JOURNAL;

ADENOSINE TRIPHOSPHATE; CELL NUCLEUS; CYTOPLASM; DNA DAMAGE; DNA REPAIR; DNA, NEOPLASM; HELA CELLS; HUMANS; LUNG NEOPLASMS; PLASMIDS; TEMPERATURE; TUMOR CELLS, CULTURED;

EID: 0031810501     PISSN: 09553002     EISSN: None     Source Type: Journal    
DOI: 10.1080/095530098142022     Document Type: Article

References (35)

1. BEYERT, N., REICHENBERGER, S., PETERS, M., HARTUNG, M., GOETTLICH, B., GOEDECKE, W., VIELMETTER, W. and PFEIFFER, P., 1994, Nonhomologous DNA end joining of synthetic hairpin substrates in Xenopus laevis egg extracts. Nucleic Acids Research, 22, 1643-1650.
2. CHEONG, N. and ILIAKIS, G., 1997, In vitro rejoining of double strand breaks induced in cellular DNA by bleomycin and restriction endonucleases. International Journal of Radiation Biology, 71, 365-375.
3. CHEONG, N., OKAYASU, R., SHAH, S., GANGULY, T., MAMMEN, P. and ILIAKIS, G., 1996, In vitro rejoining of double-strand breaks in cellular DNA by factors present in extracts of HeLa cells. International Journal of Radiation Biology, 69, 665-677.
4. DAZA, P., REICHENBERGER, S., GÖTTLICH, B., HAGMANN, M., FELDMANN, E. and PFEIFFER, P., 1996, Mechanisms of nonhomologous DNA end-joining in frogs, mice and men. Biological Chemistry, 377, 775-786.
5. DENKO, N., GIACCIA, A., PETERS, B. and STAMATO, T. D., 1989, An asymmetric field inversion gel electrophoresis method for the separation of large DNA molecules. Analytical Biochemistry, 178, 172-176.
6. DERBYSHIRE, M. K., EPSTEIN, L. H., YOUNG, C. S. H., MUNZ, P. L. and FISHEL, R., 1994, Nonhomologous recombination in human cells. Molecular and Cellular Biology, 14, 156-169.
7. FAIRMAN, M. P., JOHNSON, A. P. and THACKER, J., 1992, Multiple components are involved in the efficient joining of double stranded DNA breaks in human cell extracts. Nucleic Acids Research, 20, 4145-4152.
8. FRANKENBERG-SCHWAGER, M., 1990, Induction, repair and biological relevance of radiation-induced DNA lesions in eukaryotic cells. Radiation and Environmental Biophysics, 29, 273-292.
9. GANGULY, I. and ILIAKIS, G., 1993, A cell-free assay using cytoplasmic cell extracts to study rejoining of radiation-induced DNA double-strand breaks in human cell nuclei. International Journal of Radiation Biology, 68, 447-457.
10. GOEDECKE, W., VIELMETTER, W. and PFEIFFER, P., 1992, Activation of a system for the joining of nonhomologous DNA ends during Xenopus egg maturation. Molecular and Cellular Biology, 12, 811-816.
11. GRAWUNDER, U., WILM, M., WU, X., KULESZA, P., WILSON, T. E., MANN, M. and LIEBER, M. R., 1997, Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells. Nature, 388, 492-495.
12. ILIAKIS, G., 1991, The role of DNA double strand breaks in ionizing radiation induced killing of eukaryotic cells. BioEssays, 13, 641-648.
13. ILIAKIS, G. E., METZGER, L., DENKO, N and STAMATO, T. D., 1991b, Detection of DNA double strand breaks in synchronous cultures of CHO cells by means of asymmetric field inversion gel electrophoresis. International Journal of Radiation Biology, 59, 321-341.
14. ILIAKIS, G. E., CICILIONI, O. and METZGER, L., 1991a, Measurement of DNA double strand breaks in CHO cells at various stages of the cell cycle using pulsed field gel electrophoresis: Calibrations by means of 1251 decay. International Journal of Radiation Biology, 59, 343-357.
15. JACKSON, S. P. and JEGGO, P. A., 1995, DNA double-strand break repair and V(D)J recombination: involvement of DNA-PK. Trends in Biochemical Science, 20, 412-415.
16. JEGGO, P. A., 1990, Studies on mammalian mutants defective in rejoining double-strand breaks. Mutation Research, 239, 1-16.
17. JEGGO, P. A., TAGCIOLI, G. E. and JACKSON, S. P., 1995, Menage à trois: double strand break repair, V(D)J recombination and DNA-PK. BioEssays, 17, 949-957.
18. LEES-MILLER, S. P., 1996, The DNA-dependent protein kinase, DNA-PK: 10 years and no ends in sight. Biochem. Cell Bol., 74, 503-512.
19. LIEBER, M. R., GRAWUNDER, U., WU, X. and YANEVA, M., 1997, Tying loose ends: roles of Ku and DNA-dependent protein kinase in the repair of double-strand breaks. Current Opinions in Genetics and Development, 7, 99-104.
20. METZGER, L. and ILIAKIS, G., 1991, Kinetics of DNA double strand breaks throughout the cell cycle as assayed by pulsed field gel electrophoresis in CHO cells. International Journal of Radiation Biology, 59, 1325-1339.
21. NICOLAS, A. L., MUNZ, P. L. and YOUNG, C. S. H., 1995, A modified single-strand annealing model best explains the joining of DNA double-strand breaks in mammalian cells and cell extracts. Nucleic Acids Research, 23, 1036-1043.
22. NICOLAS, A. L. and YOUNG, C. S. H., 1994, Characterization of DNA end joining in a mammalian cell nuclear extract: Junction formation is accompanied by nucleotide loss, which is limited and uniform but not site specific. Molecular and Cellular Biology, 14, 170-180.
23. NORTH, P., GANESH, A. and THAGKER, J., 1990, The rejoining of double-strand breaks in DNA by human cell extracts. Nucleic Acids Research, 18, 6205-6210.
24. PAINTER, R. B. 1980, The role of DNA damage and repair in cell killing induced by ionizing radiation., pp. 59-68. In Radiation Biology in Cancer Research, R. E. Meyn, and H. R. Withers (Eds): Raven Press, New York.
25. PFEIFFER, P., GöTTLICH, B., REICHENBERGER, S., FELDMANN, E., DAZA, P., WARD, J. F., MILLIGAN, J. R., MULLENDERS, L. H. F. and NATARAJAN, A. T., 1996, DNA lesions and repair. Mutation Research, 366, 69-80.
26. PFEIFFER, P., THODE, S., HANCKE, J., KEOHAVONG, P. and THILLY, W. G., 1994, Resolution and conservation of mismatches in DNA end joining. Mutagenesis, 9, 527-535.
27. PFEIFFER, P. and VIELMETTER, W., 1988, Joining of nonhomologous DNA double strand breaks in vitro. Nucleic Acids Research., 16, 907-924.
28. STILLMAN, B., 1989, Initiation of eukaryotic DNA replication in vitro. Annual Rev. Cell Biol, 5, 197-245.
29. THACKER, J., CHALK, J., GANESH, A. and NORTH, P., 1992. A mechanism for deletion formation in DNA by human cell extracts: The involvement of short sequence repeats. Nucleic Acids Research, 20, 6183-6188.
30. THODE, S., SCHAFER, A., PFEIFFER, P. and VIELMETTER, W., 1990, A novel pathway of DNA end-to-end joining. Cell, 60, 921-928.
31. WANG, Y., HUQ, M. S., CHENG, X. and ILIAKIS, G., 1995, Regulation of DNA replication in irradiated cells by trans-acting factors. Radiation Research, 142, 169-175.
32. WANG, Y., HUQ, M. S. and ILIAKIS, G., 1996, Evidence for activities inhibiting in trans initiation of DNA replication in extract prepared from irradiated cells. Radiation Research, 145, 408-418.
33. WARD, J. F., 1990, The yield of DNA double-strand breaks produced intracellularly by ionizing radiation: a review. International Journal of Radiation Biology, 57, 1141-1150.
34. WEAVER, D. T., 1996, Regulation and repair of double-strand DNA breaks. Critical Reviews in Eukaryotic Gene Expression. 6, 345-375.
35. WILSON, T. E., GRAWUNDER, U. and LIEBER, M. R., 1997, Yeast DNA ligase IV mediates non-homologous DNA end joining. Nature, 388, 495-498.