In vitro rejoining of double-strand breaks in cellular DNA by factors present in extracts of HeLa cells

International Journal of Radiation Biology

Volumn 69, Issue 6, 1996, Pages 665-677

  Cheong, N ^a   Okayasu, R ^a   Shah, S ^a   Ganguly, T ^a   Mammen, P ^a   Iliakis, G ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL EXTRACT;

ARTICLE; CANCER CELL CULTURE; CONTROLLED STUDY; DNA REPAIR; DNA STRAND BREAKAGE; HELA CELL; HUMAN; HUMAN CELL; LUNG CANCER; PRIORITY JOURNAL;

ADENOSINE TRIPHOSPHATE; CELL NUCLEUS; CELL-FREE SYSTEM; DEOXYRIBONUCLEOTIDES; DNA; DNA DAMAGE; DNA REPAIR; HELA CELLS; HUMANS; POTASSIUM CHLORIDE; SODIUM CHLORIDE;

EID: 0029976544     PISSN: 09553002     EISSN: None     Source Type: Journal    
DOI: 10.1080/095530096145409     Document Type: Article

References (47)

1. ABOUSSEKHRA, A., BIGGERSTAFF, M., SHIVJI, M. K. K., VILPO, J. A., MONCOLLIN, V., PODUST, V. N., PROTIC, M., HUEBSCHER, U., EGLY, J.-M. and WOOD, R. D., 1995, Mammalian DNA nucleotide excision repair reconstituted with purified protein components. Cell, 80, 859-868.
2. AUSUBEL, F. M., BRENT, R., KINGSTON, R. E., MOORE, D. D., SEIDMAN, J. G., SMITH, J. A. and STRUHL, K., 1989, DNA-protein interactions. In Current Protocols in Molecular Biology (New York, NY: Wiley), pp. 12.0.3-1.9.
3. BADIE, C., ILIAKIS, G., FORAY, N., ALSBEIH, G., PANTELIAS, G. E., OKAYASU, R., CHEONG, N., RUSSELL, N. S., BEGG, A. C., ARLETT, C. F. and MALAISE, E. P., 1995, Defective repair of DNA double-strand breaks and chromosome damage in fibroblasts from radiosensitive leukemia patient. Cancer Research, 55, 1232-1234.
4. BASES, R., HAMORI, I., PAZZA, L., MAIO, J. and MENDEZ, F., 1990, DNA base and strand damage in X-irradiated monkey CV-1 cells: influence of pretreatment using small doses of radiation. International Journal of Radiation Biology, 58, 35-54.
5. BLUNT, T., FINNIE, N. J., TACCIOLI, G. E., SMITH, G. C. M., DEMENGEOT, J., GOTTLIEB, T. M., MIZUTA, R., VARGHESE, A. J., ALT, F. W., JEGGO, P. A. and JACKSON, S. P., 1995, Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine SCID mutation. Cell, 80, 813-823.
6. DAR, M. E. and JORGENSEN, T. J., 1995, Deletions at short direct repeats and base substitutions are characteristic mutations for bleomycin-induced double- and single-strand breaks, respectively, in a human shuttle vector system. Nucleic Acids Research, 23, 3224-3230.
7. DE JONG, J. H. R., VERMEULEN, W., KLEIN, B. and HOEIJMAKERS, H. J., 1983, Microinjection of human cell extracts corrects xeroderma pigmentosum defect. EMBO Journal, 2, 637-641.
8. DERBYSHIRE, M. K., EPSTEIN, L. H., YOUNG, C. S. H., MUNZ, P. L. and FISHEL, R. 1994, Nonhomologous recombination in human cells. Molecular and Cellular Biology, 14, 156-169.
9. FAIRMAN, M. P., JOHNSON, A. P. and THACKER, J., 1992, Multiple components are involved in the efficient joining of double stranded DNA breaks in human cell extracts. Nucleic Acids Research, 20, 4145-4152.
10. FANG, W. -H. and MODRICH, P., 1993, Human strand-specific mismatch repair occurs by a bi-directional mechanism similar to that of the bacterial reaction. Journal of Biological Chemistry, 268, 11838-11844.
11. FRANKENBERG-SCHWAGER, M., 1990, Induction, repair and biological relevance of radiation-induced DNA lesions in eukaryotic cells. Radiation and Environmental Biophysics, 29, 273-292.
12. GANGULY, T. and ILIAKIS, G., 1995, A cell-free assay using cytoplasmic cell extracts to study rejoining of radiation-induced DNA double-strand breaks in human cell nuclei. International Journal of Radiation Biology, 68, 447-458.
13. GETTS, R. and STAMATO, T. D., 1994, Absence of a Ku-like DNA end binding activity in the xrs double-strand DNA repair-deficient mutant. Journal of Biological Chemistry, 269, 15981-15984.
14. GIARD, D. J., AARONSON, S. A., TODARO, G. J., ARNSTEIN, P., KERSEY, J. H., DOSIK, H. and PARKS, W. P., 1973, In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. Journal of the National Cancer Institute, 51, 1417-1423.
15. HENNER, W. D., GRUNBERG, S. M. and HASELTINE, W. A., 1982, Sites and structure of γ radiation-induced DNA strand breaks. Journal of Biological Chemistry, 257, 11750-11754.
16. HENNER, W. D., RODRIGUEZ, L. O., HECHT, S. M. and HASELTINE, W. A., 1983, Gamma-ray induced deoxyribonucleic acid strand breaks. Journal of Biological Chemistry, 258, 711-713.
17. 125I decay. International Journal of Radiation Biology, 59, 343-357.
18. ILIAKIS, G., 1991, The role of DNA double strand breaks in ionizing radiation-induced killing of eukaryotic cells. BioEssays, 13, 641-648.
19. ILIAKIS, G., METZGER, L., DENKO, N., and STAMATO, T., 1991a, Detection of DNA double-strand breaks in synchronous cultures of CHO cells by means of asymmetric field inversion gel electrophoresis. International Journal of Radiation Biology, 59, 321-341.
20. JEGGO, P. A., 1990, Studies on mammalian mutants defective in radiation-induced DNA lesions in eukaryotic cells. Mutation Research, 239, 1-16.
21. KIRCHGESSNER, C. U., PATIL, C. K., EVANS, J. W., CUOMO, C. A., FRIED, L. M., CARTER, T., OETTINGER, M. A. and BROWN, J. M., 1995, DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science (Washington), 267, 1178-1182.
22. LEES-MILLER, S. P., GODBOUT, R., CHAN, D. W., WEINFIELD, M., DAY, R. S., III, BARRON, G. M. and ALLALUNIS-TURNER., 1995, Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line. Science (Washington), 267, 1183-1185.
23. LIEBER, M., SMITH, B., SZAKAL, A., NELSON-REES, W. and TODARO, G., 1976, A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. International Journal of Cancer, 17, 62-70.
24. LINDAHL, T. and BARNES, D. E., 1992, Mammalian DNA ligases. Annual Review of Biochemistry, 63, 251-281.
25. LOEBRICH, M., RYDBERG, B. and COOPER, P. K., 1994, DNA double-strand breaks induced by high-energy neon and iron ions in human fibroblasts. II. Probing individual Notl fragments by hybridization. Radiation Research, 139, 142-151.
26. LOPEZ, B. S., CORTEGGIANI, E., BERTRAND-MERCAT, P. and COPPEY, J., 1992, Directional recombination is initiated at a double strand break in human nuclear extracts. Nucleic Acids Research, 20, 501-506.
27. METZGER, L. and ILIAKIS, G., 1991, Kinetics of DNA double-strand break repair throughout the cell cycle as assayed by pulsed field gel electrophoresis in CHO cells. International Journal of Radiation Biology, 59, 1325-1339.
28. NICOLAS, A. L., MUNZ, P. L. and YOUNG, C. S. H., 1995, A modified single-strand annealing model best explains the joining of DNA double-strand breaks in mammalian cells and cell extracts. Nucleic Acids Research, 23, 1036-1043.
29. NICOLAS, A. L. and YOUNG, C. S. H., 1994, Characterization of DNA end joining in a mammalian cell nuclear extract: junction formation is accompanied by nucleotide loss, which is limited and uniform but not site specific. Molecular and Cellular Biology, 14, 170-180.
30. NISHIDA, C., CHOI, S. -Y., KIM, J., KEENEY, J. and LINN, S., 1988, DNA polymerases plus hela or human fibroblast cell-free extracts complement permeabilized xeroderma pigmentosum (XP) fibroblasts: application for purification of XP correcting factors. In Mechanisms and Consequences of DNA Damage Processing, edited by E.C. Friedberg and P. C. Hanawalt (New York: Liss) pp. 337-341.
31. NORTH, P., GANESH, A. and THACKER, J., 1990, The rejoining of double-strand breaks in DNA by human cell extracts. Nucleic Acids Research, 18, 6205-6210.
32. PAINTER, R. B., 1980, The role of DNA damage and repair in cell killing induced by ionizing radiation. In Radiation Biology in Cancer Research, edited by R. E. Meyn and H. R. Withers (New York, NY: Raven), pp. 59-68.
33. PFEIFFER, P. and VIELMETTER, W., 1988, Joining of nonhomologous DNA double strand breaks in vitro. Nucleic Acids Research, 16, 907-924.
34. RATHMELL, W. K. and CHU, G. A., 1994, DNA end-binding factor involved in double strand break repair and V(D)J recombination. Molecular and Cellular Biology, 14, 4741-4748.
35. RESNICK, M. A. and MARTIN, P., 1976, The repair of double-strand breaks in the nuclear DNA of Saccharomyces cerevisiae and its genetic control. Molecular and General Genetics, 143, 119-129.
36. RESNICK, M. A., ZGAGA, Z., HEITER, P., WESTMORELAND, J., FOGEL, S. and NILSSON-TILLGREN, T., 1992, Recombinational repair of diverged DNAs: a study of homoeologous chromosomes and mammalian YACs in yeast. Molecular and General Genetics, 234, 65-73.
37. ROTH, D., and WILSON, J., 1988, Illegitimate recombination in mammalian cells. In Genetic Recombination, edited by R. Kucherlapati and G. R. Smith (Washington: American Society of Microbiology), chapter 21, pp. 621-653.
38. SMIDER, V., RATHMELL, W. K., LIEBER, M. R. and CHU, G., 1994, Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science, 266, 288-291.
39. STILLMAN, B., 1989, Initiation of eukaryotic DNA replication in vitro. Annual Review of Cell Biology, 5, 197-245.
40. TACCIOLI, G., GOTTLIEB, T. M., BLUNT, T., PRIESTLY, A., DEMENGEOT, J., MIZUTA, R., LEHMANN, A., ALT, F. W., JACKSON, S. P. and JEGGO, P. A., 1994, Ku-80: product of XRCC5 gene and its role in DNA repair and V(D)J recombination. Science (Washington), 265, 1442-1445.
41. THODE, S., SCHAFER, A., PFEIFFER, P. and VIELMETTER, W., 1990, A novel pathway of DNA end-to-end joining. Cell, 60, 921-928.
42. VAN GENT, D. C., MCBLANE, J. F., RAMSDEN, D. A., SADOFSKY, M. J., HESSE, J. E. and GELLERT, M., 1995, Initiation of V(D)J recombination in a cell-free system. Cell, 81, 925-934.
43. WAGA, S., BAUER, G. and STILLMAN, B. 1994, Reconstitution of complete SV40 DNA replication with purified replication factors. Journal of Biological Chemistry, 269, 10923-10934.
44. WANG, Y., HUQ, M. S., CHENG, X. and ILIAKIS, G., 1995, Regulation of DNA replication in irradiated cells by transacting factors. Radiation Research, 142, 169-175.
45. WARD, J. F., 1990, The yield of DNA double-strand breaks produced intracellularly by ionizing radiation: a review. International Journal of Radiation Biology, 57, 1141-1150.
46. WINTERS, T. A., WEINFIELD, M. and JORGENSEN, T. J., 1992, Human HeLa cell enzymes that remove phosphoglycolate 2′-end groups from DNA. Nucleic Acids Research, 20, 2573-2580.
47. WOOD, R. D., ROBINS, P. and LINDAHL, T., 1988, Complementation of the Xeroderma Pigmentosum DNA repair defect in cell-free extracts. Cell, 53, 97-106.