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1
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0032489041
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Polymerases and the replisome: Machines within machines
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Baker TA, Bell SP. Polymerases and the replisome: machines within machines. Cell. 92:1998;295-305.
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Cell
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Baker, T.A.1
Bell, S.P.2
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3
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0024978377
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Heat shock protein-mediated disassembly of nucleoprotein structures is required for the initiation of bacteriophage lambda DNA replication
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Initiation of DNA replication in eukaryotic cells
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Dutta A, Bell SP. Initiation of DNA replication in eukaryotic cells. Annu Rev Cell Dev Biol. 13:1997;293-332.
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Dutta, A.1
Bell, S.P.2
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5
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0032472223
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Discrete start sites for DNA synthesis in the yeast ARS1 origin
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of outstanding interest. The authors have developed a highly sensitive method to map DNA replication initiation sites at the nucleotide level, replication initiation point mapping (RIP). They show that replication at the budding yeast origin ARS1 begins adjacent to ORC binding in a region known to be distorted by ORC binding both in vitro and in vivo. RIP should be sufficiently sensitive to determine exactly where bidirectional replication forks begin in fission yeast and possibly in higher eukaryotes.
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Bielinsky AK, Gerbi SA. Discrete start sites for DNA synthesis in the yeast ARS1 origin. of outstanding interest Science. 279:1998;95-98 The authors have developed a highly sensitive method to map DNA replication initiation sites at the nucleotide level, replication initiation point mapping (RIP). They show that replication at the budding yeast origin ARS1 begins adjacent to ORC binding in a region known to be distorted by ORC binding both in vitro and in vivo. RIP should be sufficiently sensitive to determine exactly where bidirectional replication forks begin in fission yeast and possibly in higher eukaryotes.
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(1998)
Science
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, pp. 95-98
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Bielinsky, A.K.1
Gerbi, S.A.2
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6
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0031278995
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Replication initiation point mapping
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Gerbi SA, Bielinsky AK. Replication initiation point mapping. Methods. 13:1997;271-280.
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Methods
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Gerbi, S.A.1
Bielinsky, A.K.2
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7
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0026607331
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ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex
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Bell SP, Stillman B. ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex. Nature. 357:1992;128-134.
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Nature
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Bell, S.P.1
Stillman, B.2
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Protein-DNA interactions at a yeast replication origin
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Diffley JF, Cocker JH. Protein-DNA interactions at a yeast replication origin. Nature. 357:1992;169-172.
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Nature
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Diffley, J.F.1
Cocker, J.H.2
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9
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0030712877
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Architecture of the yeast origin recognition complex bound to origins of DNA replication
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of special interest. Extensive characterization of ORC binding to ARS1 in vitro shows that ORC interacts preferentially with one DNA strand. This paper provides evidence for two groups of ORC subunits making contact with two different regions of the ARS1. In budding yeast, ORC functions as a single complex, but findings in this paper introduce the idea that ORC might function as multiple complexes in some organisms (see [12])
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Lee DG, Bell SP. Architecture of the yeast origin recognition complex bound to origins of DNA replication. of special interest Mol Cell Biol. 17:1997;7159-7168 Extensive characterization of ORC binding to ARS1 in vitro shows that ORC interacts preferentially with one DNA strand. This paper provides evidence for two groups of ORC subunits making contact with two different regions of the ARS1. In budding yeast, ORC functions as a single complex, but findings in this paper introduce the idea that ORC might function as multiple complexes in some organisms (see [12]).
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Mol Cell Biol
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Lee, D.G.1
Bell, S.P.2
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10
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0029586090
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Conserved initiator proteins in eukaryotes
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Gavin KA, Hidaka M, Stillman B. Conserved initiator proteins in eukaryotes. Science. 270:1995;1667-1671.
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Science
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Gavin, K.A.1
Hidaka, M.2
Stillman, B.3
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11
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0031438125
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Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: Human homologue (ORC5L) maps to 7q22
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Ishiai M, Dean FB, Okumura K, Abe M, Moon KY, Amin AA, Kagotani K, Taguchi H, Murakami Y, Hanaoka F, et al. Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22. Genomics. 46:1997;294-298.
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Genomics
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Ishiai, M.1
Dean, F.B.2
Okumura, K.3
Abe, M.4
Moon, K.Y.5
Amin, A.A.6
Kagotani, K.7
Taguchi, H.8
Murakami, Y.9
Hanaoka, F.10
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12
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0030712354
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Identification of HsORC4, a member of the human origin of replication recognition complex
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of special interest. Human Orc4 was identified (HsORC4) based on sequence similarity with budding yeast Orc4. Interestingly, HsORC4 reveals an unsuspected homology to the ORC1/Cdc18 family, and HsORC4 has a putative nucleotide triphosphate binding motif that is lacking in budding yeast Orc4. Experiments in this paper suggest that distinct ORC subcomplexes may be present in human cells.
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Quintana DG, Hou Z, Thome KC, Hendricks M, Saha P, Dutta A. Identification of HsORC4, a member of the human origin of replication recognition complex. of special interest J Biol Chem. 272:1997;28247-28251 Human Orc4 was identified (HsORC4) based on sequence similarity with budding yeast Orc4. Interestingly, HsORC4 reveals an unsuspected homology to the ORC1/Cdc18 family, and HsORC4 has a putative nucleotide triphosphate binding motif that is lacking in budding yeast Orc4. Experiments in this paper suggest that distinct ORC subcomplexes may be present in human cells.
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J Biol Chem
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Quintana, D.G.1
Hou, Z.2
Thome, K.C.3
Hendricks, M.4
Saha, P.5
Dutta, A.6
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13
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0030592518
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Interaction between the origin recognition complex and the replication licensing system in Xenopus
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Rowles A, Chong JPJ, Brown L, Howell M, Evan GI, Blow JJ. Interaction between the origin recognition complex and the replication licensing system in Xenopus. Cell. 87:1996;287-296.
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Rowles, A.1
Chong, J.P.J.2
Brown, L.3
Howell, M.4
Evan, G.I.5
Blow, J.J.6
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14
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0030294685
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The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin
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Romanowski P, Madine MA, Rowles A, Blow JJ, Laskey RA. The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin. Curr Biol. 6:1996;1416-1425.
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Curr Biol
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Romanowski, P.1
Madine, M.A.2
Rowles, A.3
Blow, J.J.4
Laskey, R.A.5
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15
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0030271388
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The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts
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Coleman TR, Carpenter PB, Dunphy WG. The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts. Cell. 87:1996;53-63.
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Coleman, T.R.1
Carpenter, P.B.2
Dunphy, W.G.3
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0030951331
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Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast
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Donovan S, Harwood J, Drury LS, Diffley JF. Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast. Proc Natl Acad Sci USA. 94:1997;5611-5616.
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Proc Natl Acad Sci USA
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Donovan, S.1
Harwood, J.2
Drury, L.S.3
Diffley, J.F.4
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Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs.
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2 chromatin.
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2 chromatin.
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Cell
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Tanaka, T.1
Knapp, D.2
Nasmyth, K.3
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0030886099
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Components and dynamics of DNA replication complexes in S. cerevisiae: Redistribution of MCM proteins and Cdc45p during S phase
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of outstanding interest. Chromatin co-immunoprecipitation was used to detect specific DNA sequences bound by replication factors in budding yeast. To do this, formaldehyde cross-linking is used to covalently link proteins to chromatin in vivo, cells are lysed, chromatin is sheared, and the replication factor(s) of interest are immunoprecipitated. Cross-links are then hydrolyzed and co-precipitating DNA is detected by the polymerase chain reaction. The authors showed that ORC and MCMs are components of the pre-RC, and that both ORC and Cdc6 are required for origin association of MCMs. Most significantly, they showed that MCM proteins and Cdc45 become associated with non-origin DNA during S phase suggesting that MCMs and Cdc45 move with the replication fork.
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Aparicio OM, Weinstein DM, Bell SP. Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase. of outstanding interest Cell. 91:1997;59-69 Chromatin co-immunoprecipitation was used to detect specific DNA sequences bound by replication factors in budding yeast. To do this, formaldehyde cross-linking is used to covalently link proteins to chromatin in vivo, cells are lysed, chromatin is sheared, and the replication factor(s) of interest are immunoprecipitated. Cross-links are then hydrolyzed and co-precipitating DNA is detected by the polymerase chain reaction. The authors showed that ORC and MCMs are components of the pre-RC, and that both ORC and Cdc6 are required for origin association of MCMs. Most significantly, they showed that MCM proteins and Cdc45 become associated with non-origin DNA during S phase suggesting that MCMs and Cdc45 move with the replication fork.
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(1997)
Cell
, vol.91
, pp. 59-69
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Aparicio, O.M.1
Weinstein, D.M.2
Bell, S.P.3
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19
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0032567761
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Identification of a preinitiation step in DNA replication that is independent of origin recognition complex and cdc6, but dependent on cdk2
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of special interest. This paper provides evidence that XCdc6 and ORC can be removed from chromatin once MCMs are 'loaded' and the MCMs will remain. It also suggests that CDK activation of replication initiation targets a relatively late step in replication initiation. This step could be activation of origin unwinding by an MCM helicase or assembly of replication fork components with the helicase.
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Hua XH, Newport J. Identification of a preinitiation step in DNA replication that is independent of origin recognition complex and cdc6, but dependent on cdk2. of special interest J Cell Biol. 140:1998;271-281 This paper provides evidence that XCdc6 and ORC can be removed from chromatin once MCMs are 'loaded' and the MCMs will remain. It also suggests that CDK activation of replication initiation targets a relatively late step in replication initiation. This step could be activation of origin unwinding by an MCM helicase or assembly of replication fork components with the helicase.
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(1998)
J Cell Biol
, vol.140
, pp. 271-281
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Hua, X.H.1
Newport, J.2
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20
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0032110626
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Nucleotide-dependent prereplicative complex assembly by Cdc6p, a homolog of eukaryotic and prokaryotic clamp-loaders
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of outstanding interest. Analysis of cdc6 mutants suggests that ATP binding is needed for Cdc6p to bind ORC/origins whereas ATP hydrolysis is required for Cdc6p mediated loading of MCMs onto DNA. Interestingly, the authors identify conserved sequence motifs between the Cdc6 family of proteins (Cdc6, Orc1, Orc4) and RFC which is the clamp-loader for the ring shaped processivity factor PCNA. Similarity appears to extend to prokaryotic counterparts of RFC. The authors propose that Cdc6p is a clamp-loader for MCMs.
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Perkins G, Diffley JF. Nucleotide-dependent prereplicative complex assembly by Cdc6p, a homolog of eukaryotic and prokaryotic clamp-loaders. of outstanding interest Mol Cell. 2:1998;23-32 Analysis of cdc6 mutants suggests that ATP binding is needed for Cdc6p to bind ORC/origins whereas ATP hydrolysis is required for Cdc6p mediated loading of MCMs onto DNA. Interestingly, the authors identify conserved sequence motifs between the Cdc6 family of proteins (Cdc6, Orc1, Orc4) and RFC which is the clamp-loader for the ring shaped processivity factor PCNA. Similarity appears to extend to prokaryotic counterparts of RFC. The authors propose that Cdc6p is a clamp-loader for MCMs.
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(1998)
Mol Cell
, vol.2
, pp. 23-32
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Perkins, G.1
Diffley, J.F.2
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21
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0031184659
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A globular complex formation by Nda1 and the other five members of the MCM protein family in fission yeast
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of special interest. The heterohexameric complex of MCMs was purified from fission yeast. Electron microscopy showed the complex to be globular with a central cavity.
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Adachi Y, Usukura J, Yanagida M. A globular complex formation by Nda1 and the other five members of the MCM protein family in fission yeast. of special interest Genes Cells. 2:1997;467-479 The heterohexameric complex of MCMs was purified from fission yeast. Electron microscopy showed the complex to be globular with a central cavity.
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(1997)
Genes Cells
, vol.2
, pp. 467-479
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Adachi, Y.1
Usukura, J.2
Yanagida, M.3
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22
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0026559546
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Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein
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Thommes P, Fett R, Schray B, Burkhart R, Barnes M, Kennedy C, Brown NC, Knippers R. Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein. Nucleic Acids Res. 20:1992;1069-1074.
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Thommes, P.1
Fett, R.2
Schray, B.3
Burkhart, R.4
Barnes, M.5
Kennedy, C.6
Brown, N.C.7
Knippers, R.8
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23
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MCM proteins: Evolution, properties, and role in DNA replication
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Kearsey SE, Labib K. MCM proteins: evolution, properties, and role in DNA replication. Biochim Biophys Acta. 1398:1998;113-136.
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Kearsey, S.E.1
Labib, K.2
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24
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0030859463
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A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex
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of outstanding interest. This is the first report that MCMs have ATPase and DNA helicase activities in vitro.
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Ishimi Y. A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex. of outstanding interest J Biol Chem. 272:1997;24508-24513 This is the first report that MCMs have ATPase and DNA helicase activities in vitro.
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J Biol Chem
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Ishimi, Y.1
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25
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0030070356
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Coupling of a replicative polymerase and helicase: A tau-DnaB interaction mediates rapid replication fork movement
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Kim S, Dallmann HG, McHenry CS, Marians KJ. Coupling of a replicative polymerase and helicase: a tau-DnaB interaction mediates rapid replication fork movement. Cell. 84:1996;643-650.
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Kim, S.1
Dallmann, H.G.2
McHenry, C.S.3
Marians, K.J.4
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26
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0032478695
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Biochemical function of mouse minichromosome maintenance 2 protein
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of outstanding interest. This extends characterization of the helicase function of MCMs. MCM2 specifically inhibited activity of an MCM4 - MCM6 - MCM7 complex. The authors also show that the amino terminus of MCM2 interacts with histone H3. They suggest that different MCMs may have different functions in initiation.
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Ishimi Y, Komamura Y, You Z, Kimura H. Biochemical function of mouse minichromosome maintenance 2 protein. of outstanding interest J Biol Chem. 273:1998;8369-8375 This extends characterization of the helicase function of MCMs. MCM2 specifically inhibited activity of an MCM4 - MCM6 - MCM7 complex. The authors also show that the amino terminus of MCM2 interacts with histone H3. They suggest that different MCMs may have different functions in initiation.
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(1998)
J Biol Chem
, vol.273
, pp. 8369-8375
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Ishimi, Y.1
Komamura, Y.2
You, Z.3
Kimura, H.4
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Putting it all together: Building a prereplicative complex
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Newlon CS. Putting it all together: building a prereplicative complex. Cell. 91:1997;717-720.
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(1997)
Cell
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Newlon, C.S.1
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0029761706
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Physical interactions among Mcm proteins and effects of Mcm dosage on DNA replication in Saccharomyces cerevisiae
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Lei M, Kawasaki Y, Tye BK. Physical interactions among Mcm proteins and effects of Mcm dosage on DNA replication in Saccharomyces cerevisiae. Mol Cell Biol. 16:1996;5081-5090.
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Mol Cell Biol
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Lei, M.1
Kawasaki, Y.2
Tye, B.K.3
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29
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0032520181
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Cdc7 is required throughout the yeast S phase to activate replication origins
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of outstanding interest. This paper together with [30], shows that Cdc7 in budding yeast activates early and late origins separately with late origins requiring Cdc7 activity later in S phase. Replication of early and late origins was measured after a brief pulse of Cdc7 activity early in S phase and under conditions of low Cdc7 activity throughout S phase. The results suggest that late origins do not require a higher activity of Cdc7 but only require that Cdc7 be active later in S phase.
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Donaldson AD, Fangman WL, Brewer BJ. Cdc7 is required throughout the yeast S phase to activate replication origins. of outstanding interest Genes Dev. 12:1998;491-501 This paper together with [30], shows that Cdc7 in budding yeast activates early and late origins separately with late origins requiring Cdc7 activity later in S phase. Replication of early and late origins was measured after a brief pulse of Cdc7 activity early in S phase and under conditions of low Cdc7 activity throughout S phase. The results suggest that late origins do not require a higher activity of Cdc7 but only require that Cdc7 be active later in S phase.
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(1998)
Genes Dev
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, pp. 491-501
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Donaldson, A.D.1
Fangman, W.L.2
Brewer, B.J.3
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30
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0032519615
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The Cdc7 protein kinase is required for origin firing during S phase
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of outstanding interest. This paper together with [29] shows that Cdc7 is needed throughout S phase for firing of individual replication origins in budding yeast. Limiting Cdc7 function results in slow progression through S phase rather that a delayed entry into S phase. After a hydroxyurea block, Cdc7 is required for activation of late firing origins and conversion of pre-RC complexes to the post-RC form at those origins.
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Bousset K, Diffley JF. The Cdc7 protein kinase is required for origin firing during S phase. of outstanding interest Genes Dev. 12:1998;480-490 This paper together with [29] shows that Cdc7 is needed throughout S phase for firing of individual replication origins in budding yeast. Limiting Cdc7 function results in slow progression through S phase rather that a delayed entry into S phase. After a hydroxyurea block, Cdc7 is required for activation of late firing origins and conversion of pre-RC complexes to the post-RC form at those origins.
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(1998)
Genes Dev
, vol.12
, pp. 480-490
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Bousset, K.1
Diffley, J.F.2
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31
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0027978640
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Interaction of Dbf4, the Cdc7 protein kinase regulatory subunit, with yeast replication origins in vivo
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Dowell SJ, Romanowski P, Diffley JF. Interaction of Dbf4, the Cdc7 protein kinase regulatory subunit, with yeast replication origins in vivo. Science. 265:1994;1243-1246.
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Dowell, S.J.1
Romanowski, P.2
Diffley, J.F.3
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Characterization of an essential Orc2p-associated factor that plays a role in DNA replication
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Hardy CF. Characterization of an essential Orc2p-associated factor that plays a role in DNA replication. Mol Cell Biol. 16:1996;1832-1841.
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Hardy, C.F.1
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Hsk1+, a Schizosaccharomyces pombe gene related to Saccharomyces cerevisiae CDC7, is required for chromosomal replication
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Masai H, Miyake T, Arai K-I. hsk1+, a Schizosaccharomyces pombe gene related to Saccharomyces cerevisiae CDC7, is required for chromosomal replication. EMBO J. 14:1995;3094-3104.
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Masai, H.1
Miyake, T.2
Arai K-I3
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34
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0030877320
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Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: In vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7
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of special interest. Human Cdc7 is shown to preferentially phosphorylate MCM2 and weakly phosphorylate MCM3 in vitro.
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Sato N, Arai K, Masai H. Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7. of special interest EMBO J. 16:1997;4340-4351 Human Cdc7 is shown to preferentially phosphorylate MCM2 and weakly phosphorylate MCM3 in vitro.
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EMBO J
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, pp. 4340-4351
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Sato, N.1
Arai, K.2
Masai, H.3
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35
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0031447170
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Identification and characterization of a human protein kinase related to budding yeast Cdc7p
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Jiang W, Hunter T. Identification and characterization of a human protein kinase related to budding yeast Cdc7p. Proc Natl Acad Sci USA. 94:1997;14320-14325.
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Proc Natl Acad Sci USA
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Jiang, W.1
Hunter, T.2
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0027192941
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Cell cycle regulation of the yeast Cdc7 protein kinase by association with the Dbf4 protein
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Jackson AL, Pahl PM, Harrison K, Rosamond J, Sclafani RA. Cell cycle regulation of the yeast Cdc7 protein kinase by association with the Dbf4 protein. Mol Cell Biol. 13:1993;2899-2908.
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Jackson, A.L.1
Pahl, P.M.2
Harrison, K.3
Rosamond, J.4
Sclafani, R.A.5
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37
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0032555483
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Purification of Hsk1, a minichromosome maintenance protein kinase from fission yeast
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of special interest. Purification of the fission yeast homolog of Cdc7 (Hsk1) reveals many similarities with budding yeast Cdc7 including the requirement for an activating subunit. Purified Hsk1 phosphorylates MCM2 in vitro.
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Brown GW, Kelly TJ. Purification of Hsk1, a minichromosome maintenance protein kinase from fission yeast. of special interest J Biol Chem. 273:1998;22083-22090 Purification of the fission yeast homolog of Cdc7 (Hsk1) reveals many similarities with budding yeast Cdc7 including the requirement for an activating subunit. Purified Hsk1 phosphorylates MCM2 in vitro.
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J Biol Chem
, vol.273
, pp. 22083-22090
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Brown, G.W.1
Kelly, T.J.2
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38
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0031435940
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Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis
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of outstanding interest. This paper provides the most complete evidence to date that Mcm2 is the phosphorylation target of Cdc7. In vivo phosphorylation of Mcm2 depends on Cdc7; Mcm2 is an in vitro substrate of Cdc7 and is preferentially phosphorylated relative to other Mcms; and genetic interactions suggest a direct interaction between Mcm2 and Cdc7-Dbf4.
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Lei M, Kawasaki Y, Young MR, Kihara M, Sugino A, Tye BK. Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis. of outstanding interest Genes Dev. 11:1997;3365-3374 This paper provides the most complete evidence to date that Mcm2 is the phosphorylation target of Cdc7. In vivo phosphorylation of Mcm2 depends on Cdc7; Mcm2 is an in vitro substrate of Cdc7 and is preferentially phosphorylated relative to other Mcms; and genetic interactions suggest a direct interaction between Mcm2 and Cdc7-Dbf4.
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(1997)
Genes Dev
, vol.11
, pp. 3365-3374
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Lei, M.1
Kawasaki, Y.2
Young, M.R.3
Kihara, M.4
Sugino, A.5
Tye, B.K.6
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39
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0031006539
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Mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p
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of special interest. Identification of a recessive mcm5 mutant that bypasses the requirement for Cdc7 kinase is the strongest genetic evidence that Cdc7 function targets MCMs.
-
Hardy CF, Dryga O, Seematter S, Pahl PM, Sclafani RA. mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p. of special interest Proc Natl Acad Sci USA. 94:1997;3151-3155 Identification of a recessive mcm5 mutant that bypasses the requirement for Cdc7 kinase is the strongest genetic evidence that Cdc7 function targets MCMs.
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Proc Natl Acad Sci USA
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Hardy, C.F.1
Dryga, O.2
Seematter, S.3
Pahl, P.M.4
Sclafani, R.A.5
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40
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0030054354
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Interaction of Cdc2 and Cdc18 with a fission yeast ORC2-like protein
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Leatherwood J, Lopez-Girona A, Russell P. Interaction of Cdc2 and Cdc18 with a fission yeast ORC2-like protein. Nature. 379:1996;360-363.
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Leatherwood, J.1
Lopez-Girona, A.2
Russell, P.3
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41
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0030906338
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Cyclin-dependent kinase and initiation at eukaryotic origins: A replication switch?
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Jallepalli PV, Kelly TJ. Cyclin-dependent kinase and initiation at eukaryotic origins: a replication switch? Curr Opin Cell Biol. 9:1997;358-363.
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(1997)
Curr Opin Cell Biol
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Jallepalli, P.V.1
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42
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0030053520
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Role for a Xenopus Orc2-related protein in controlling DNA replication
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Carpenter PB, Mueller PR, Dunphy WG. Role for a Xenopus Orc2-related protein in controlling DNA replication. Nature. 379:1996;357-360.
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Nature
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Carpenter, P.B.1
Mueller, P.R.2
Dunphy, W.G.3
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43
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0029906578
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Interaction between yeast Cdc6 protein and B-type cyclin/Cdc28 kinases
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Elsasser S, Lou F, Wang B, Campbell JL, Jong A. Interaction between yeast Cdc6 protein and B-type cyclin/Cdc28 kinases. Mol Biol Cell. 7:1996;1723-1735.
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Elsasser, S.1
Lou, F.2
Wang, B.3
Campbell, J.L.4
Jong, A.5
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44
-
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0030727157
-
Regulation of the replication initiator protein p65cdc18 by CDK phosphorylation
-
of outstanding interest. This paper provides genetic and biochemical evidence that fission yeast Cdc18 is down-regulated by CDK phosphorylation in vivo. Phosphorylated Cdc18 is targeted for proteolysis. This is the first example of a cellular initiation factor directly regulated in vivo by CDK-dependent phosphorylation and proteolysis.
-
Jallepalli PV, Brown GW, Muzi-Falconi M, Tien D, Kelly TJ. Regulation of the replication initiator protein p65cdc18 by CDK phosphorylation. of outstanding interest Genes Dev. 11:1997;2767-2779 This paper provides genetic and biochemical evidence that fission yeast Cdc18 is down-regulated by CDK phosphorylation in vivo. Phosphorylated Cdc18 is targeted for proteolysis. This is the first example of a cellular initiation factor directly regulated in vivo by CDK-dependent phosphorylation and proteolysis.
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(1997)
Genes Dev
, vol.11
, pp. 2767-2779
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Jallepalli, P.V.1
Brown, G.W.2
Muzi-Falconi, M.3
Tien, D.4
Kelly, T.J.5
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45
-
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0031885041
-
Negative regulation of Cdc18 DNA replication protein by Cdc2
-
of outstanding interest. Together with [44] and [57] this paper provides evidence that fission yeast Cdc18 binds to and is phosphorylated by CDKs. Cdc18 is multiply phoshorylated; phosphorylation of Thr104 appears to inhibits Cdc18 by a mechanism independent of proteolysis but other phosphorylations do not have this effect.
-
Lopez-Girona A, Mondesert O, Leatherwood J, Russell P. Negative regulation of Cdc18 DNA replication protein by Cdc2. of outstanding interest Mol Biol Cell. 9:1998;63-73 Together with [44] and [57] this paper provides evidence that fission yeast Cdc18 binds to and is phosphorylated by CDKs. Cdc18 is multiply phoshorylated; phosphorylation of Thr104 appears to inhibits Cdc18 by a mechanism independent of proteolysis but other phosphorylations do not have this effect.
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(1998)
Mol Biol Cell
, vol.9
, pp. 63-73
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Lopez-Girona, A.1
Mondesert, O.2
Leatherwood, J.3
Russell, P.4
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46
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0029861615
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Phosphorylation of Mcm4 by Cdc2 protein kinase inhibits the activity of the minichromosome maintenance complex
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Hendrickson M, Madine M, Dalton S, Gautier J. Phosphorylation of Mcm4 by Cdc2 protein kinase inhibits the activity of the minichromosome maintenance complex. Proc Natl Acad Sci USA. 93:1996;12223-12228.
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Hendrickson, M.1
Madine, M.2
Dalton, S.3
Gautier, J.4
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47
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0028859923
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Cell cycle-dependent phosphorylation and dephosphorylation of the yeast DNA polymerase alpha-primase B subunit
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Foiani M, Liberi G, Lucchini G, Plevani P. Cell cycle-dependent phosphorylation and dephosphorylation of the yeast DNA polymerase alpha-primase B subunit. Mol Cell Biol. 15:1995;883-891.
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Mol Cell Biol
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Foiani, M.1
Liberi, G.2
Lucchini, G.3
Plevani, P.4
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48
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0032527284
-
Evidence for a Cdc6p-independent mitotic resetting event involving DNA polymerase alpha
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of special interest. This paper provides evidence that polymerase alpha primase association with chromatin is regulated by CDK phosphorylation in budding yeast. How this influences DNA replication has yet to be determined. This study reveals a novel pathway by which CDKs might control DNA replication.
-
Desdouets C, Santocanale C, Drury LS, Perkins G, Foiani M, Plevani P, Diffley JF. Evidence for a Cdc6p-independent mitotic resetting event involving DNA polymerase alpha. of special interest EMBO J. 17:1998;4139-4146 This paper provides evidence that polymerase alpha primase association with chromatin is regulated by CDK phosphorylation in budding yeast. How this influences DNA replication has yet to be determined. This study reveals a novel pathway by which CDKs might control DNA replication.
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(1998)
EMBO J
, vol.17
, pp. 4139-4146
-
-
Desdouets, C.1
Santocanale, C.2
Drury, L.S.3
Perkins, G.4
Foiani, M.5
Plevani, P.6
Diffley, J.F.7
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49
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0026539144
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Cdc2 family kinases phosphorylate a human cell DNA replication factor, RPA, and activate DNA replication
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Dutta A, Stillman B. Cdc2 family kinases phosphorylate a human cell DNA replication factor, RPA, and activate DNA replication. EMBO J. 11:1992;2189-2199.
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(1992)
EMBO J
, vol.11
, pp. 2189-2199
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Dutta, A.1
Stillman, B.2
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50
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0027479493
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Regulation of Saccharomyces cerevisiae CDC7 function during the cell cycle
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Yoon HJ, Loo S, Campbell JL. Regulation of Saccharomyces cerevisiae CDC7 function during the cell cycle. Mol Biol Cell. 4:1993;195-208.
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(1993)
Mol Biol Cell
, vol.4
, pp. 195-208
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Yoon, H.J.1
Loo, S.2
Campbell, J.L.3
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51
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0032562610
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Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin
-
of outstanding interest. This paper provides some of the first evidence about how CDKs may activate DNA replication initiation. The authors show that Cdc45 binding to chromatin depends on prior association of MCMs and most importantly that Cdc45 binding requires activation of S phase CDK function. Their data also show that Cdc45 binding to Mcm2 is cell cycle regulated.
-
Zou L, Stillman B. Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. of outstanding interest Science. 280:1998;593-596 This paper provides some of the first evidence about how CDKs may activate DNA replication initiation. The authors show that Cdc45 binding to chromatin depends on prior association of MCMs and most importantly that Cdc45 binding requires activation of S phase CDK function. Their data also show that Cdc45 binding to Mcm2 is cell cycle regulated.
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(1998)
Science
, vol.280
, pp. 593-596
-
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Zou, L.1
Stillman, B.2
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52
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0031455421
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Persistent initiation of DNA replication and chromatin-bound MCM proteins during the cell cycle in cdc6 mutants
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2 is one of the primary mechanisms for limiting DNA replication to once per cell cycle.
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2 is one of the primary mechanisms for limiting DNA replication to once per cell cycle.
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(1997)
Genes Dev
, vol.11
, pp. 3375-3386
-
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Liang, C.1
Stillman, B.2
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53
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0032478176
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1 phase is sufficient to block prereplicative complex formation in Saccharomyces cerevisiae
-
of special interest. This paper provides direct evidence that mitotic CDK activity can inhibit pre-RC formation in budding yeast by blocking the function of Cdc6.
-
1 phase is sufficient to block prereplicative complex formation in Saccharomyces cerevisiae. of special interest Proc Natl Acad Sci USA. 95:1998;2384-2389 This paper provides direct evidence that mitotic CDK activity can inhibit pre-RC formation in budding yeast by blocking the function of Cdc6.
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(1998)
Proc Natl Acad Sci USA
, vol.95
, pp. 2384-2389
-
-
Detweiler, C.S.1
Li, J.J.2
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54
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0032015702
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Regulated chromosomal DNA replication in the absence of a nucleus
-
of outstanding interest. Using Xenopus extracts, the authors developed a completely soluble system for DNA replication that faithfully recapitulates once per cell cycle control of replication. In this study, re-replication appears to be inhibited by a nuclear factor which prevents re-binding of MCMs to chromatin. This study is important for reconciling observations in Xenopus extracts that led to the licensing factor hypothesis with genetic observations in yeasts.
-
Walter J, Sun L, Newport J. Regulated chromosomal DNA replication in the absence of a nucleus. of outstanding interest Mol Cell. 1:1998;519-529 Using Xenopus extracts, the authors developed a completely soluble system for DNA replication that faithfully recapitulates once per cell cycle control of replication. In this study, re-replication appears to be inhibited by a nuclear factor which prevents re-binding of MCMs to chromatin. This study is important for reconciling observations in Xenopus extracts that led to the licensing factor hypothesis with genetic observations in yeasts.
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(1998)
Mol Cell
, vol.1
, pp. 519-529
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Walter, J.1
Sun, L.2
Newport, J.3
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55
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0029761435
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1 defines a 'point of no return' after which Cdc6 synthesis cannot promote DNA replication in yeast
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1 defines a 'point of no return' after which Cdc6 synthesis cannot promote DNA replication in yeast. Genes Dev. 10:1996;1516-1531.
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(1996)
Genes Dev
, vol.10
, pp. 1516-1531
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Piatti, S.1
Bohm, T.2
Cocker, J.H.3
Diffley, J.F.4
Nasmyth, K.5
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57
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0030924762
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Interaction of the S phase regulator cdc18 with cyclin-dependent kinase in fission yeast
-
of special interest. The authors show that fission yeast Cdc18 binds to CDKs via the amino-terminal region of Cdc18.
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Brown GW, Jallepalli PV, Huneycutt BJ, Kelly TJ. Interaction of the S phase regulator cdc18 with cyclin-dependent kinase in fission yeast. of special interest Proc Natl Acad Sci USA. 94:1997;6142-6147 The authors show that fission yeast Cdc18 binds to CDKs via the amino-terminal region of Cdc18.
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(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 6142-6147
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-
Brown, G.W.1
Jallepalli, P.V.2
Huneycutt, B.J.3
Kelly, T.J.4
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58
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0030950644
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Fission yeast WD-repeat protein pop1 regulates genome ploidy through ubiquitin-proteasome-mediated degradation of the CDK inhibitor Rum1 and the S-phase initiator Cdc18
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of outstanding interest. The authors identify a protein, Pop1, that binds Cdc18 and is needed for Cdc18 ubiquitination and resulting proteolysis. Cdc18 degradation helps to ensures once per cell cycle control of replication. Pop1 is similar to budding yeast Cdc4 which is required for ubiquitin mediated proteolysis of budding yeast Cdc6 [56].
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Kominami K, Toda T. Fission yeast WD-repeat protein pop1 regulates genome ploidy through ubiquitin-proteasome-mediated degradation of the CDK inhibitor Rum1 and the S-phase initiator Cdc18. of outstanding interest Genes Dev. 11:1997;1548-1560 The authors identify a protein, Pop1, that binds Cdc18 and is needed for Cdc18 ubiquitination and resulting proteolysis. Cdc18 degradation helps to ensures once per cell cycle control of replication. Pop1 is similar to budding yeast Cdc4 which is required for ubiquitin mediated proteolysis of budding yeast Cdc6 [56].
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(1997)
Genes Dev
, vol.11
, pp. 1548-1560
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Kominami, K.1
Toda, T.2
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59
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0031921561
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Human CDC6/Cdc18 associates with Orc1 and cyclin-cdk and is selectively eliminated from the nucleus at the onset of S phase
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of special interest. This paper provides evidence that human Cdc18 (hCdc18) binds to CDKs similar to observations of Cdc18 in fission yeast and Cdc6 in budding yeast. This paper reveals a possible new mechanism for regulation of Cdc6/Cdc18 activity that has not been seen in yeasts: hCdc18 may be regulated by changes in its nuclear localization rather than by proteolysis. This paper also provides evidence for sub-complexes of ORC subunits in human cells.
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Saha P, Chen J, Thome KC, Lawlis SJ, Hou ZH, Hendricks M, Parvin JD, Dutta A. Human CDC6/Cdc18 associates with Orc1 and cyclin-cdk and is selectively eliminated from the nucleus at the onset of S phase. of special interest Mol Cell Biol. 18:1998;2758-2767 This paper provides evidence that human Cdc18 (hCdc18) binds to CDKs similar to observations of Cdc18 in fission yeast and Cdc6 in budding yeast. This paper reveals a possible new mechanism for regulation of Cdc6/Cdc18 activity that has not been seen in yeasts: hCdc18 may be regulated by changes in its nuclear localization rather than by proteolysis. This paper also provides evidence for sub-complexes of ORC subunits in human cells.
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(1998)
Mol Cell Biol
, vol.18
, pp. 2758-2767
-
-
Saha, P.1
Chen, J.2
Thome, K.C.3
Lawlis, S.J.4
Hou, Z.H.5
Hendricks, M.6
Parvin, J.D.7
Dutta, A.8
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60
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0032493273
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Sud1 (+) targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast
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of outstanding interest. In this study, a pop1 related gene, sud1, is also found to be important for Cdc18 ubiquitination that results in Cdc18 proteolysis. It is shown that sud1 specifically recognizes phosphorylated Cdc18 which accounts for the observation that CDK phosphorylation leads to Cdc18 degradation. It is not clear why both pop1 and sud1 are important for this process in fission yeast.
-
Jallepalli PV, Tien D, Kelly TJ. sud1 (+) targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast. of outstanding interest Proc Natl Acad Sci USA. 95:1998;8159-8164 In this study, a pop1 related gene, sud1, is also found to be important for Cdc18 ubiquitination that results in Cdc18 proteolysis. It is shown that sud1 specifically recognizes phosphorylated Cdc18 which accounts for the observation that CDK phosphorylation leads to Cdc18 degradation. It is not clear why both pop1 and sud1 are important for this process in fission yeast.
-
(1998)
Proc Natl Acad Sci USA
, vol.95
, pp. 8159-8164
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Jallepalli, P.V.1
Tien, D.2
Kelly, T.J.3
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61
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0031945518
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Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7
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of special interest. Interaction between Rb and MCM7 was identified in a two-hybrid screen, and Rb as well as p130 inhibited DNA replication in Xenopus extracts.
-
Sterner JM, Dew-Knight S, Musahl C, Kornbluth S, Horowitz JM. Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7. of special interest Mol Cell Biol. 18:1998;2748-2757 Interaction between Rb and MCM7 was identified in a two-hybrid screen, and Rb as well as p130 inhibited DNA replication in Xenopus extracts.
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(1998)
Mol Cell Biol
, vol.18
, pp. 2748-2757
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Sterner, J.M.1
Dew-Knight, S.2
Musahl, C.3
Kornbluth, S.4
Horowitz, J.M.5
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62
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0032511148
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Geminin, an inhibitor of DNA replication, is degraded during mitosis
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1 which are permissive for pre-RC formation. One of the essential mitotic events is cyclin proteolysis which inactivates CDKs. The authors identified a novel protein, geminin, which is degraded by the same pathway as cyclins. Overexpression of this protein in Xenopus inhibits DNA replication but does not stop subsequent cell divisions. The authors propose that geminin is a specific DNA replication inhibitor that is present in higher eukaryotes but lacking in yeasts.
-
1 which are permissive for pre-RC formation. One of the essential mitotic events is cyclin proteolysis which inactivates CDKs. The authors identified a novel protein, geminin, which is degraded by the same pathway as cyclins. Overexpression of this protein in Xenopus inhibits DNA replication but does not stop subsequent cell divisions. The authors propose that geminin is a specific DNA replication inhibitor that is present in higher eukaryotes but lacking in yeasts.
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(1998)
Cell
, vol.93
, pp. 1043-1053
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McGarry, T.J.1
Kirschner, M.W.2
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63
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0030839209
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1 arrested yeast cells to inhibit B-type cyclin accumulation and to prevent uncontrolled entry into S-phase
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1 is important to prevent premature initiation of DNA replication.
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1 is important to prevent premature initiation of DNA replication.
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(1997)
J Cell Sci
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, pp. 1523-1531
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Irniger, S.1
Nasmyth, K.2
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64
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0031991836
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CDC16 controls initiation at chromosome replication origins
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of special interest. Mutations that affect mitosis can cause misregulation of DNA replication during the next cell cycle. This paper confirms claims from an earlier paper by the same authors that CDC16 is required to prevent inappropriate firing of replication origins. CDC16 is a subunit of the anaphase promoting complex which is needed for mitosis specific ubiquitin mediated proteolysis of many factors including cyclins. The exact link between CDC16 and replication is not clear.
-
Heichman KA, Roberts JM. CDC16 controls initiation at chromosome replication origins. of special interest Mol Cell. 1:1998;457-463 Mutations that affect mitosis can cause misregulation of DNA replication during the next cell cycle. This paper confirms claims from an earlier paper by the same authors that CDC16 is required to prevent inappropriate firing of replication origins. CDC16 is a subunit of the anaphase promoting complex which is needed for mitosis specific ubiquitin mediated proteolysis of many factors including cyclins. The exact link between CDC16 and replication is not clear.
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(1998)
Mol Cell
, vol.1
, pp. 457-463
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Heichman, K.A.1
Roberts, J.M.2
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65
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0029960469
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Cdc45p assembles into a complex with Cdc46p/Mcm5p, is required for minichromosome maintenance, and is essential for chromosomal DNA replication
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Hopwood B, Dalton S. Cdc45p assembles into a complex with Cdc46p/Mcm5p, is required for minichromosome maintenance, and is essential for chromosomal DNA replication. Proc Natl Acad Sci USA. 93:1996;12309-12314.
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Hopwood, B.1
Dalton, S.2
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66
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0031056052
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CDC45, a novel yeast gene that functions with the origin recognition complex and Mcm proteins in initiation of DNA replication
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Zou L, Mitchell J, Stillman B. CDC45, a novel yeast gene that functions with the origin recognition complex and Mcm proteins in initiation of DNA replication. Mol Cell Biol. 17:1997;553-563.
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(1997)
Mol Cell Biol
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Zou, L.1
Mitchell, J.2
Stillman, B.3
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67
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0030894095
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Identification of Cdc45p, an essential factor required for DNA replication
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Hardy CF. Identification of Cdc45p, an essential factor required for DNA replication. Gene. 187:1997;239-246.
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Gene
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Hardy, C.F.1
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69
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0031878711
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Identification of sna41 gene, which is the suppressor of nda4 mutation and is involved in DNA replication in Schizosaccharomyces pombe
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Miyake S, Yamashita S. Identification of sna41 gene, which is the suppressor of nda4 mutation and is involved in DNA replication in Schizosaccharomyces pombe. Genes Cells. 3:1998;157-166.
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(1998)
Genes Cells
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Miyake, S.1
Yamashita, S.2
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70
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0030831599
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CDC45 is required in conjunction with CDC7/DBF4 to trigger the initiation of DNA replication
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of special interest. The authors show that budding yeast Cdc45 carries out its essential function after S phase CDKs are active. This differs from Cdc6/Cdc18 assembly of MCMs which must occur before S phase CDK activation. Furthermore, they show that Cdc45 function and Cdc7 - Dbf4 kinase are dependent on one another for function.
-
Owens JC, Detweiler CS, Li JJ. CDC45 is required in conjunction with CDC7/DBF4 to trigger the initiation of DNA replication. of special interest Proc Natl Acad Sci USA. 94:1997;12521-12526 The authors show that budding yeast Cdc45 carries out its essential function after S phase CDKs are active. This differs from Cdc6/Cdc18 assembly of MCMs which must occur before S phase CDK activation. Furthermore, they show that Cdc45 function and Cdc7 - Dbf4 kinase are dependent on one another for function.
-
(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 12521-12526
-
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Owens, J.C.1
Detweiler, C.S.2
Li, J.J.3
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71
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0031027479
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Molecular cloning of a gene required for DNA replication in Ustilago maydis
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Onel K, Holloman WK. Molecular cloning of a gene required for DNA replication in Ustilago maydis. Mol Gen Genet. 253:1997;463-468.
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Mol Gen Genet
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Onel, K.1
Holloman, W.K.2
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72
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0032541155
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The human homolog of Saccharomyces cerevisiae CDC45
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Saha P, Thome KC, Yamaguchi R, Hou Z, Weremowicz S, Dutta A. The human homolog of Saccharomyces cerevisiae CDC45. J Biol Chem. 273:1998;18205-18209.
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(1998)
J Biol Chem
, vol.273
, pp. 18205-18209
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Saha, P.1
Thome, K.C.2
Yamaguchi, R.3
Hou, Z.4
Weremowicz, S.5
Dutta, A.6
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