The agouti gene product inhibits lipolysis in human adipocytes via a Ca2+-dependent mechanism

FASEB Journal

Volumn 12, Issue 13, 1998, Pages 1391-1396

  Xue, Bingzhong ^a,b   Moustaid Moussa, Naima ^a,b   Wilkison, William O ^a,b   Zemel, Michael B ^a,b  

^a UNIVERSITY OF TENNESSEE   (United States)

^b Zen Bio Inc   (United States)

Author keywords

Agouti polypeptide; Calcium; Melanocortin receptor; MSH

Indexed keywords

ADENYLATE CYCLASE; AGOUTI PROTEIN; ARGIPRESSIN; CALCIUM ION; CORTICOTROPIN; CORTICOTROPIN RECEPTOR; FORSKOLIN; GENE PRODUCT; MELANOCORTIN RECEPTOR; NITRENDIPINE; POTASSIUM CHLORIDE; UNCLASSIFIED DRUG;

ADIPOCYTE; ARTICLE; CALCIUM CELL LEVEL; CONTROLLED STUDY; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; LIPOLYSIS; OBESITY; PRIORITY JOURNAL;

EID: 0031718256     PISSN: 08926638     EISSN: None     Source Type: Journal    
DOI: 10.1096/fasebj.12.13.1391     Document Type: Article

References (36)

1. Jackson, I. J. (1991) Mouse coat color mutations: a molecular genetic resource which spans the centuries. BioEssays 13, 439-446
2. Galbraith, D. B. (1964) The agouti pigment pattern of the mouse: a quantitative and experimental study. J. Exp. Zool. 155, 71-90
3. vy/- mice: ectopic expression of the agouti gene. FASEB J. 8, 479-488
4. Klebig, M. L., Wilkinson, J. E., and Woychik, R. P. (1994) Molecular analysis of the mouse agouti gene and the role of dominant agouti locus mutations in obesity and insulin resistance. In Molecular and Genetic Aspects of Obesity (Bray, G., ed) Louisiana State Univ. Press, Baton Rouge
5. w-,A-,and aa hosts. J. Exp. Zool. 137, 189-196
6. Silvers, W. K. (1979) The agouti and extension series of alleles, umbrous and sable. In The Coat Colors of Mice, pp.6-44, Springer Verlag, New York
7. Lu, D., Willard, D., Patel, I. R., Kadwell, S., Overton, L., Kost, T., Luther, M., Chen, W., Woychik, R. P., Wilkison, W. O., and Cone, R. D. (1994) Agouti protein is an antagonist of the melanocyte-stimulating hormone receptor. Nature (London) 371, 799-802
8. Yang, Y. K., Ollmann, M. M., Wilson, B. D., Dickinson, C.,Yamada, T., Barsh, G. S., and Gantz, I. (1997) Effects of recombinant agouti-signaling protein on melanocortin action. Mol. Endocrinol. 11, 274-280
9. Hunt, G., and Thody, A. J. (1995) Agouti protein can act independently of melanocyte-stimulating hormone to inhibit melanogenesis. J. Endocrinol. 147, R1-R4
10. Sakai, C., Ollmann, M., Kobayashi, T., Abdel-Malek, Z., Muller, J., Vieira, W. D., Imokawa, G., Barsh, G. S., and Hearing, V. (1997) Modulation of marine melanocyte function in vitro by agouti signal protein. EMBO J. 16, 3544-3552
11. Kim, J. H., Kiefer, L. L., Woychik, R. P., Wilkison, W. O., Truesdale, A., Ittoop, O., Willard, D., Nichols, J., and Zemel, M. B. (1997) Agouti regulation of intracellular calcium. Role of melanocortin receptor. Am. J. Physiol. 272, E379-E384
12. Zemel, M. B., Moore, J. W., Moustaid, N., Kim, J. H., Nichols, J. S., Blanchard, S. G., Parks, D., Harris, C., Lee, F. W., Grizzle, M., James, M., and Wilkison, W. O. (1998) Effects of a potent melanocortin agonist on the diabetic/obese phenotype in yellow mice. Int. J. Obesity In press
13. Jones, B. H., Kim, J. H.,Zemel, M. B., Woychik, R. P., Michaud, E. J., Wilkison, W. O., and Moustaid, N. (1996) Upregulation of adipocyte metabolism by agouti protein: possible paracrine actions in yellow mouse obesity. Am. J. Physiol. 270, E192-E196
14. i) results in stimulation of fatty acid synthase. Obesity Res. 3, 338s (abstr.)
15. Kim, J. H., Mynatt, R. L., Moore, J. W., Woychik, R. P., Moustaid, N., and Zemel, M. B. (1996) The effects of calcium channel blockade on agouti induced obesity. FASEB J. 10, 1646-1652
16. Kwon, H. Y., Bultman, S. J., Loffler, C., Chen, W. J., Furdon, P. J., Powell, J. G., Usala, A. L., Wilkison, W., Hansmann, I., and Woychik, R. P. (1994) Molecular structure and chromosomal mapping of the human homologue of the agouti gene. Proc. Natl. Acad. Sci. USA 91, 9760-9764
17. Draznin, B., Susaman, K. E., Eckel, R. H., Kao, M., Yost, T., and Sherman, N.A. (1988) Possible role of cytosolic free calcium concentrations in mediating insulin resistance of obesity and hyper-insulinemia. J. Clin. Invest. 82, 1848-1852,
18. Draznin, B., Sussman, K., Kao K., Lewis, D., and Sherman, N. (1987) The existence of an optimal range of cytosolic free calcium for insulin-stimulated glucose transport in rat adipocytes. J. Biol. Chem. 262, 14385-14388
19. Byyny, R. L., Loverde, M., Lloyd, S., Metchell, W., and Draznin, B. (1992) Cytosolic calcium and insulin resistance in elderly patients with essential hypertension. Am. J. Hypertension 5, 459-464
20. Yen, T. T., Mckee, M. M., and Stamm, N. B. (1984) Thermogenesis and weight control Int. J. Obesity 8 (Suppl. 1), 65-78
21. Yen, T. T., Steinmetz, J., and Wolff, G. L. (1970) Lipolysis in genetically obese and diabetes-prone mice. Horm. Metab. Res. 2, 200-203
22. vy a mice by LY79711, a phenethanolamine, and stereoisomers. Life Sci. 32, 1515-1522
23. Tebar, F., Soley, M., and Ramirez, I. (1996) The antilipolytic effects of insulin and epidermal growth factor in rat adipocytes are mediated by different mechanisms. Endocrinology 137, 4181-4188
24. Boston, B. A., and Cone, R. D. (1996) Characterization of melanocortin receptor subtype expression in murine adipose tissues and in 3T3-L1 cell line. Endocrinology 137, 2043-2050
25. Oelofsen, W., and Ramachandran, J. (1983) Studies of corticotropin receptors on rat adipocytes. Arch. Biochem. Biophys. 225, 414-421
26. Moustaid, N., Jones, B. H., and Taylor, J. W. (1996) Insulin increases lipogenic enzyme activity in cultured human adipocytes. J. Nutr. 126, 865-870
27. Bultman, S. J., Michaud, E. J., and Woychik, R. P. (1992) Molecular characterisation of the mouse agouti locus. Cell 71, 1195-1204
28. Willard, D. H., Bodnar, W., Harris, C., Kiefer, L., Nichols, J. S., Blanchard, S., Hoffman, C., Moyer, M., Burkhart, W., Weiel, J., Luther, M. A., Wilkison, W. O., and Rocque, W. J. (1993) Agouti structure and function: characterization of a potent α-melanocyte stimulating hormone receptor antagonist. Biochemistry 34, 12341-12346
29. Boobis, L. H., and Manghan, R. J. (1983) A simple one-step enzymatic fluorometric method for the determination of glycerol in 20 μl of plasma. Clin. Chim. Acta 132, 173-179
30. Huszar D., Lynch, C. A., Fairchild-Huntress, V., Dunmore, J. H., Fang, Q., Berhemeier, L. R., Gu, W., Kesterson, R. A., Boston, B. A., Cone, R. D., Smith, F. J., Campfield, L. A., Burn, P., and Lee, F. (1997) Targeted disruption of the melanocortin-4 receptor results in obesity in mice. Cell 88,131-141
31. Fan, W., Boston, B. A., Kesterson, R. A., Hruby, V. J., and Cone, R. D. (1997) Role of melanocortinergic in feeding and the agouti obesity syndrome. Nature (London) 385, 165-168
32. Steeley, R. J., Yagaloff, K. A., Fisher, S. L., Burn, P., Thiele, T. E., van Dijk, G., Baskin, D. G., and Schwartz, M. (1997) Melanocortin receptors in leptin effects. Nature (London) 390, 349
33. Boston, B. A., Blaydon, K. M., Varnerin, J., and Cone, R. D. (1997) Independent and additive effects of central POMC and leptin pathways on marine obesity. Science 278, 1641-1644
34. 2+ in corticotropin-stimulated white adipocytes. Am. J. Physiol. 266, E418-E420
35. 2+ in adrenocorticotropic hormone-stimulated lipolysis in the perifused fat cell system. J. Biol Chem. 249, 2000-2004
36. Claycombe, K. H., Jones, B. H., Standrige, M. K., Wilkison, W. O., Zemel, M. B., Guo, Y. S., and Moustaid, N. (1997) Transcriptional regulation of the adipocyte fatty acid synthase gene by the agouti gene product: interaction with insulin. FASEB J. 11, A352 (abstr.)