Mutual conversion of fatty-acid substrate specificity by a single amino-acid exchange at position 527 in P-450Cm2 and P-450Alk3A

European Journal of Biochemistry

Volumn 256, Issue 2, 1998, Pages 398-403

  Zimmer, Thomas ^a,b,c   Scheller, Ulrich ^a   Takagi, Masamichi ^b   Schunck, Wolf Hagen ^a  

^a MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^b UNIVERSITY OF TOKYO   (Japan)

^c FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

Author keywords

Active site; Cytochrome P 450; Fatty acid substrate specificity; Site directed mutagenesis

Indexed keywords

CYTOCHROME P450 ISOENZYME; FATTY ACID; LAURIC ACID; OXYGENASE; PALMITIC ACID;

AMINO ACID COMPOSITION; ARTICLE; ENZYME ACTIVITY; ENZYME SPECIFICITY; GENE CONSTRUCT; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; YEAST;

AMINO ACID SEQUENCE; BINDING SITES; CANDIDA; CYTOCHROME P-450 ENZYME SYSTEM; FATTY ACIDS; HYDROXYLATION; LAURIC ACIDS; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PALMITIC ACID; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); CANDIDA MALTOSA; EUKARYOTA;

EID: 0031718198     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2560398.x     Document Type: Article

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