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The Homothorax homeoprotein activates the nuclear localization of another homeoprotein, extradenticle, and suppresses eye development in Drosophila
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Bürglin T: Analysis of TALE superclass homeobox genes (MEIS, PBC, KNOX, Iroquois, TGIF) reveals a novel domain conserved between plants and animals. Nucleic Acids Res 1997, 25:4173-4180.
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Meis proteins are major in vivo DNA binding partners for wild-type but not chimeric Pbx proteins
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Chang CP, Jacobs Y, Nakamura T, Jenkins NA, Copeland NG, Cleary ML: Meis proteins are major in vivo DNA binding partners for wild-type but not chimeric Pbx proteins. Mol Cell Biol 1997, 17:5679-5687. Like Hth and Exd, Meis1 and Pbx can directly bind to each other in vitro and appear to be stable partners in cells. DNA-binding sites were selected for Meis1 alone and Meis1 + Pbx1 dimers. Interestingly, Pbx1-containing complexes isolated from cells select the binding site TGATTGAC (also shown in [18]), which is the same sequence that was selected by Meis1 + Pbx1. These results suggest that a large fraction of the Pbx1 in cells is complexed with a Meis-like protein. The region of Pbx required for the interaction with Meis1 is mapped to PBC-A domain, amino-terminal to the E2a fusion site in Pbx (Figure 1).
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Mol Cell Biol
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40
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Meis1 and pKnox1 bind DNA cooperatively with Pbx1 utilizing an interaction surface disrupted in oncoprotein E2a-Pbx1
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•], this paper shows that Meis1-Pbx1 dimers form on the site TGATTGAC and that this interaction requires the PBC-A domain. Similar interactions were shown to occur between pKnox1/Prep1 and Pbx1. In addition, this paper presents evidence that a hydrophobic heptad repeat in the HM domain of Meis1 is necessary for the interaction with Pbx1 (Figure 1).
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Shen W-F, Montgomery J, Rozenfeld S, Moskow J, Lawrence H, Buchberg A, Largman C: AbdB-like Hox proteins stabilize DNA binding by the Meis1 homeodomain proteins. Mol Cell Biol 1997, 17:6448-6458.
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Chang CP, de Vivo I, Cleary ML: The Hox cooperativity motif of the chimeric oncoprotein E2a-Pbx1 is necessary and sufficient for oncogenesis. Mol Cell Biol 1997, 17:81-88.
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