Age-associated decline in ascorbic acid concentration, recycling, and biosyntheis in rat hepatocytes - Reversal with (R)-α-lipoic acid supplementation

FASEB Journal

Volumn 12, Issue 12, 1998, Pages 1183-1189

  Lykkesfeldt, Jens ^a,b   Hagen, Tory M ^a,c   Vinarsky, Vladimir ^a   Ames, Bruce N ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c OREGON STATE UNIVERSITY   (United States)

Author keywords

Aging; Biomarker; Oxidative stress

Indexed keywords

ANTIOXIDANT; ASCORBIC ACID; BIOLOGICAL MARKER; TERT BUTYL HYDROPEROXIDE; THIOCTIC ACID;

AGED; AGING; ANIMAL CELL; ANTIOXIDANT ACTIVITY; ARTICLE; BIOSYNTHESIS; CONTROLLED STUDY; DIET SUPPLEMENTATION; LIVER CELL; MALE; MITOCHONDRIAL RESPIRATION; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; RAT;

EID: 0031682205     PISSN: 08926638     EISSN: None     Source Type: Journal    
DOI: 10.1096/fasebj.12.12.1183     Document Type: Article

References (48)

1. Shigenaga, M. K., Hagen, T. M., and Ames, B. N. (1994) Oxidative damage and mitochondrial decay in aging. Proc. Natl. Acad. Sci. USA 91, 10771-10778
2. Sohal, R. S., and Weindruch, R. (1996) Oxidative stress, caloric restriction, and aging. Science 273, 59-63
3. Hagen, T. M., Yowe, D. L, Bartholomew, J. C., Wehr, C. M., Do, K. L., Park, J. Y., and Ames, B. N. (1997) Mitochondrial decay in hepatocytes from old rats: membrane potential declines, heterogeneity and oxidants increase. Proc. Natl. Acad. Sci. USA 94, 3064-3069
4. de la Asuncion, J. G., Millan, A., Pla, R., Bruseghini, L., Esteras, A., Pallardo, F. V., Sastre, J., and Vina, J. (1996) Mitochondrial glutathione oxidation correlates with age-associated oxidative damage to mitochondrial DNA. FASEB J. 10, 333-338
5. Berlett, B. S., and Stadtman, E. R. (1997) Protein oxidation in aging, disease, and oxidative stress. J. Biol. Chem. 272, 20313-20316
6. Helbock, H. J., Beckman, K. B., Shigenaga, M. K., Walter, P. B., Woodall, A. A., Yeo, H. C., and Ames, B. N. (1998) DNA oxidation matters: The HPLC-electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine. Proc. Natl. Acad. Sci. USA 95, 288-293
7. Ames, B. N., Shigenaga, M. K., and Hagen, T. M. (1993) Oxidants, antioxidants, and the degenerative diseases of aging. Proc. Natl. Acad. Sci. USA 90, 7915-7922
8. Sastre, J., Pallardo, F. V., Pla, R., Pellin, A., Juan, G., O'Connor, J. E., Estrela, J. M., Miquel, J., and Vina, J. (1996) Aging of the liver: age-associated mitochondrial damage in intact hepatocytes. Hepatology 24, 1199-1205
9. Beckman, K. B., and Ames, B. N. (1998) The free radical theory of aging matures. Physiol. Rev. 71, in press
10. Frei, B., England, L., and Ames, B. N. (1989) Ascorbate is an outstanding antioxidant in human blood plasma. Proc. Natl. Acad. Sci. USA 86, 6377-6381
11. Lykkesfeldt, J., Loft, S., and Poulsen, H. E. (1995) Determination of ascorbic acid and dehydroascorbic acid in plasma by high- performance liquid chromatography with coulometric detection - are they reliable biomarkers of oxidative stress? Anal. Biochem. 229, 329-335
12. Lykkesfeldt, J., Prieme, H., Loft, S., and Poulsen, H. E. (1996) Effect of smoking cessation on plasma ascorbic acid concentration. Brit. Med. J. 313, 91
13. Lykkesfeldt, J., Loft, S., Nielsen, J. B., and Poulsen, H. E. (1997) Ascorbic acid and dehydroascorbic acid as biomarkers of oxidative stress caused by smoking. Am. J. Clin. Nutr. 65, 959-963
14. Barja, G. (1996) Ascorbic acid and aging. In "Subcellular biochemistry" (J. R. Harris, Ed.), Vol. 25, pp. 157-188. Plenum Press, New York
15. Rikans, L. E., and Moore, D. R. (1988) Effect of aging on aqueous-phase antioxidants in tissues of male Fisher rats. Biochim. Biophys. Acta 966, 269-275
16. Yu, B. P., Laganiere, S., and Kim, J. W. (1989) Influence of life-prolonging food restriction on membrane lipoperoxidation and antioxidant status. In "Oxygen radicals in biology and medicine" (M. G. Simic, K. A. Taylor, J. F. Ward, and G. von Sonntag, Eds.), pp. 1067-1073. Plenum Press, New York
17. Rikans, L. E., Lopez, T. R., and Hornbrook, K. R. (1996) Age and gender differences in hepatic ascorbic acid concentrations and NADPH-dehydroascorbic acid reductasc activity. Mech. Ageing Develop. 91, 165-169
18. De, A. K., and Darad, R. (1991) Age-associated chenges in antioxidants and antioxidative enzymes in rats. Mech. Ageing Develop. 59, 123-128
19. Devasagayam, T. P. A. (1986) Senescence-associated decrease of NADPH-induced lipid peroxidation in rat liver microsomes. FEBS Lett. 205, 246-250
20. Eklow, L., Moldeus, P., and Orrenius, S. (1984) Oxidation of ghuathione during hydroperoxide metabolism. A study using isolated hepatocytes and the glutathione reductase inhibitor 1,3-bis(2-chloroethyl)-1-nitrospurea. Eur. J. Biochem. 138, 459-463
21. Scholich, H., Murphy, M. E., and Sies, H. (1989) Antioxidant activity of dihydrolipoate against microsomal lipid peroxidation and its dependence on alpha-tocopherol. Biochim. Biophys. Acta 1001, 256-261
22. Suzuki, Y. J., Tsuchiya, M., and Packer, L. (1993) Antioxidant activities of dihydrolipoic acid and its structural homologues. Free Radical Res. Comm. 18, 115-122
23. Packer, L. (1994) Antioxidant properties of lipoic acid and its therapeutic effects in prevention of diabetes complications and cataracts. Ann. N. Y. Acad. Sci. 738, 257-264
24. Scott, B. C., Aruoma, O. I., Evans, P. J., O'Neill, C., Van der Vliet, A., Cross, G. E., Tritschler, H., and Halliwell, B. (1994) Lipoic and dihydrolipoic acids as antioxidants. A critical evaluation. Free Radical Res. 20, 119-133
25. Packer, L., Witt, E. H., and Tritschler, H. J. (1995) α-Lipoic acid as a biological antioxidant. Free Radical Biol. Med. 19, 227-250
26. Packer, L., Tritschler, H. J., and Wessel, K. (1997) Neuroprotection by the metabolic antioxidant alpha-lipoic acid. Free Radical Biol. Med. 22, 359-378
27. Packer, L., Roy, S., and Sen, C. K. (1997) Alpha-lipoic acid: a metabolic antioxidant and potential redox modulator of transcription. Adv. Pharmacol. 38, 79-101
28. Moldéus, P., Hogberg, J., and Orrenius, S. (1978) Isolation and use of liver cells. Meth. Enzymol. 52, 60-71
29. Banhegyi, G., Csala, M., Braun, L., Garzo, T., and Mandl, J. (1996) Ascorbate synthesis-dependent glutathione consumption in mouse liver. FEBS Lett. 381, 39-41
30. Braun, L., Csala, M., Poussu, A., Garzo, T., Mandl, J., and Banhegyi, G. (1996) Glutathione depletion induces glycogenolysis dependent ascorbate synthesis in isolated murine hepatocytes. FEBS Lett. 388, 173-176
31. Bellomo, G., Jewell, S. A., Thor, H., and Orrenius, S. (1982) Regulation of intracellular calcium compartmentation: studies with isolated hepatocytes and t-butyl hydroperoxide. Proc. Natl. Acad. Sci. USA 79, 6842-6846
32. 2+ efflux and membrane damage during t-butylhydroperoxide metabolism by liver mitochondria. FEBS Lett. 153, 289-292
33. 2+ stores. FEBS Lett. 168, 38-42
34. Maitra, I., Serbinova, E., Trischler, H., and Packer, L. (1995) Alpha-lipoic acid prevents buthionine sulfoximine-induced cataract formation in newborn rats. Free Radical Biol. Med. 18, 823-829
35. Xu, D. P., and Wells, W. W. (1996) α-Lipoic acid dependent regeneration of ascorbic acid from dehydroascorbic acid in rat liver mitochondria. J. Bioenerg. Biomembr. 28, 77-85
36. Kitazawa, M., Podda, M., Thiele, J., Traber, M. G., Iwasaki, K., Sakamoto, K., and Packer, L. (1997) Interactions between vitamin E homologues and ascorbate free radicals in murine skin homogenates irradiated with ultraviolet light. Photochem. Photobiol. 65, 355-365
37. Roy, S., Sen, C. K., Tritschler, H. J., and Packer, L. (1997) Modulation of cellular reducing equivalent homeostasis by alpha-lipoic acid. Mechanisms and implications for diabetes and ischemic injury. Biochem. Pharmacol. 53, 393-399
38. Searls, R. L., and Sanadi, D. R. (1960) alpha-Ketoglutaric Dehydrogenase. J. Biol. Chem. 235, 2485-2491
39. Haramaki, N., Han, D., Handelman, G. J., Tritschler, H. J., and Packer, L. (1997) Cytosolic and mitochondrial systems for NADH- and NADPH-dependent reduction of alpha-lipoic acid. Free Radical Biol. Med. 22, 535-542
40. Banhegyi, G., Braun, L., Csala, M., Puskas, F., and Mandl, J. (1997) Ascorbate metabolism and its regulation in animals. Free Radical Biol. Med. 23, 793-803
41. Bluck, L. J., Izzard, A. P., and Bates, C. J. (1996) Measurement of ascorbic acid kinetics in man using stable isotopes and gas Chromatography/mass spectrometry. J. Mass Spectr. 31, 741-748
42. Tsao, C. S., and Young, M. (1989) Effect of exogenous ascorbic acid intake on biosynthesis of ascorbic acid in mice. Life Sci. 45, 1553-1557
43. Sato, P., Nishikimi, M., and Udenfriend, S. (1976) Is L-gulonolactone-oxidase the only enzyme missing in animals subject to scurvy? Biochem. Biophys. Res. Comm. 71, 293-299
44. Nishikimi, M. (1979) L-Gulono-gamma-lactone oxidase (rat and goat liver). Meth. Enzymol. 62, 24-30
45. Haramaki, N., Assadnazari, H., Zimmer, G., Schepkin, V., and Packer, L. (1995) The influence of vitamin E and dihydrolipoic acid on cardiac energy and glutathione status under hypoxiareoxygenation. Biochem. Mol. Biol. Int. 37, 591-597
46. Sen, C. K., Roy, S., Man, D., and Packer, L. (1997) Regulation of cellular thiols in human lymphocytes by alpha-lipoic acid: a flow cytometric analysis. Free Radical Biol. Med. 22, 1241-1257
47. Han, D., Handelman, G., Marcocci, L., Sen, C. K., Roy, S., Kobuchi, H., Tritschler, H. J., Flohe, L., and Packer, L. (1997) Lipoic acid increases de novo synthesis of cellular glutathione by improving cystine utilization. Biofactors 6, 321-338
48. Miquel, J. (1992) An update on the mitochondrial-DNA mutation hypothesis of cell aging. Mutat Res. 275, 209-216