Formation and function of Spemann's organizer

Annual Review of Cell and Developmental Biology

Volumn 13, Issue , 1997, Pages 611-667

  Harland, Richard ^a   Gerhart, John ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

BMP signaling; Dorsalization of mesoderm; Meso endoderm induction; Neural induction; Wnt signaling

Indexed keywords

PROTEIN KINASE; TRANSCRIPTION FACTOR;

ANIMAL CELL; BLASTULA; CELL DIFFERENTIATION; CHROMOSOME NOR; EMBRYO; EMBRYO DEVELOPMENT; GASTRULATION; GENE DISRUPTION; NERVOUS SYSTEM DEVELOPMENT; NONHUMAN; OOCYTE DEVELOPMENT; PRIORITY JOURNAL; REVIEW; SIGNAL TRANSDUCTION; SPEMANN ORGANIZER; TRANSCRIPTION REGULATION; XENOPUS;

AMPHIBIA; ANIMALS; BLASTOCYST; CELL DIFFERENTIATION; ENDODERM; GASTRULA; MESODERM; SIGNAL TRANSDUCTION;

AMPHIBIA; ANIMALIA; VERTEBRATA;

EID: 0031469373     PISSN: 10810706     EISSN: None     Source Type: Book Series    
DOI: 10.1146/annurev.cellbio.13.1.611     Document Type: Review

References (252)

1. Albano RM, Arkell R, Beddington RS, Smith JC. 1994. Expression of inhibin subunits and follistatin during postimplantation mouse development: decidual expression of activin and expression of follistatin in primitive streak, somites and hindbrain. Development 120:803-13
2. Albers B. 1987. Competence as the main factor determining the size of the neural plate. Dev. Growth Differ. 29:535-45
3. Amaya E, Musci TJ, Kirschner MW. 1991. Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos. Cell 66:257-70
4. Amaya E, Stein PA, Musci TJ, Kirschner MW. 1993. FGF signalling in the early specification of mesoderm in Xenopus. Development 118:477-87
5. Aono A, Hazama M, Notoya K, Taketomi S, Yamasaki H, et al. 1995. Potent ectopic bone-inducing activity of bone morphogenetic protein-4/7 heterodimer. Biochem. Biophys. Res. Commun. 210:670-77
6. Asashima M, Nakano H, Shimada K, Kinoshita K, Ishii K, et al. 1990. Mesodermal induction in early amphibian embryos by activin A (erythroid differentiation factor). Roux's Arch. Dev. Biol. 198:330-35
7. Baker JC, Harland RM, 1996. A novel mesoderm inducer, Madr2, functions in the ac-tivin signal transduction pathway. Genes Dev. 10:1880-89
8. Ban-Holtfreter H. 1965. Differentiation capacities of Spemann's organizer investigated in explants of diminishing size. PhD thesis. University of Michigan, Ann Arbor. pp. 252.
9. Barth LG. 1941. Neural differentiation without an organizer. J. Exp. Zool. 87:371-84
10. Barth LG, Barth LJ. 1974. Ionic regulation of embryonic induction and cell differentiation in Rana pipiens. Dev. Biol. 39:1-22
11. Behrens J, Von Kries JP, Kuehl M, Bruhn L, Wedlich D, et al. 1996. Functional interaction of β-catenin with the transcription factor LEF-1. Nature 382:638-42
12. Bejcek BE, Li DY, Deuel TF. 1989. Transformation by v-sis occurs by an internal autoactivation mechanism. Science 245:1496-99
13. Blitz IL, Cho KW. 1995. Anterior neurectoderm is progressively induced during gastrulation: the role of the Xenopus homeobox gene orthodenticle. Development 121:993-1004
14. Bolce ME, Hemmati-Brivanlou A, Kushner PD, Harland RM. 1992. Ventral ectoderm of Xenopus forms neural tissue, including hindbrain, in response to activin. Development 115:681-68
15. Boterenbrood EC, Nieuwkoop PD. 1973. The formation of the mesoderm in Urodelan amphibians: V. Its regional induction by the endoderm. Wilhelm Roux's Arch. 173:319-32
16. Boucaut JC, Clavilier L, Darribere T, Delarue M, Riou JF, Shi DL. 1996. What mechanisms drive cell migration and cell interactions in Pleurodeles? Int. J. Dev. Biol. 40:675-83
17. Bouwmeester T, Kim S, Sasai Y, Lu B, De Robertis EM. 1996. Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer. Nature 382:595-601
18. Carnac G, Kodjabachian L, Gurdon JB, Lemaire P. 1996. The homeobox gene siamois is a target of the Wnt dorsalisation pathway and triggers organiser activity in the absence of mesoderm. Development 122:3055-65
19. Chang C, Wilson P, Mathews LS, Hemmati-Brivanlou A. 1997. A Xenopus type I activin receptor mediates mesodermal but not neural specification during embryogenesis. Development 124:827-37
20. Chen X, Rubock MJ, Whitman M. 1996. A transcriptional partner for MAD proteins in TGF-β signalling. Nature 383:691-96
21. Chitnis A, Henrique D, Lewis J, Ish-Horowicz D, Kintner C. 1995. Primary neurogenesis in Xenopus embryos regulated by a homologue of the Drosophila neurogenic gene Delta. Nature 375:761-66
22. Christian JL, McMahon JA, McMahon AP, Moon RT. 1991. XWnt-8 a Xenopus Wnt-1/int-I-related gene responsive to mesoderm-inducing growth factors, may play a role in ventral mesodermal patterning during embryogenesis. Development 111:1045-55
23. Christian JL, Moon RT. 1993. Interactions between Xwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus. Genes Dev. 7:13-28
24. Christian JL, Olson DJ, Moon RT. 1992. Xwnt-8 modifies the character of mesoderm induced by bFGF in isolated Xenopus ectoderm. EMBO J. 11:33-41
25. Cooke J. 1985. Dynamics of the control of body pattern in the development of Xenopus laevis. III. Timing and pattern after U.V. irradiation of the egg and after excision of presumptive head endo-mesoderm. J. Embryol. Exp. Morphol. 88:135-80
26. Cooke J, Smith JC. 1987. The midblastula cell cycle transition and the character of mesoderm in UV-induced nonaxial Xenopus development. Development 99:197-210
27. Cornell RA, Kimelman D. 1994. Activin-mediated mesoderm induction requires FGF. Development 120:453-62
28. Cornell RA, Musci TJ, Kimelman D. 1995. FGF is a prospective competence factor for early activin-type signals in Xenopus mesoderm induction. Development 121:2429-37
29. Cox WG, Hemmati-Brivanlou A. 1995. Caudalization of neural fate by tissue recombination and bFGF. Development 121:4349-58
30. Cui Y, Brown JD, Moon RT, Christian JL. 1995. Xwnt-8b: a maternally expressed Xenopus Wnt gene with a potential role in establishing the dorsoventral axis. Development 121:2177-86
31. Dale L, Howes G, Price BM, Smith JC. 1992. Bone morphogenetic protein 4: a ventralizing factor in early Xenopus development. Development 115:573-85
32. Dale L, Slack JM. 1987a. Fate map for the 32-cell stage of Xenopus laevis. Development 99:527-51
33. Dale L, Slack JM. 1987b. Regional specification within the mesoderm of early embryos of Xenopus laevis. Development 100:279-95
34. Dale L, Smith JC, Slack JM. 1985. Mesoderm induction in Xenopus laevis: a quantitative study using a cell lineage label and tissue-specific antibodies. J. Embryol. Exp. Morphol. 89:289-312
35. Darnell DK, Schoenwolf GC. 1995. Dorsoventral patterning of the avian mesencephalon/metencephalon: role of the notochord and floor plate in suppressing Engrailed-2. J. Neurobiol. 26:62-74
36. Darnell DK, Schoenwolf GC, Ordahl CP. 1992. Changes in dorsoventral but not rostrocaudal regionalization of the chick neural tube in the absence of cranial notochord, as revealed by expression of Engrailed-2. Dev. Dyn. 193:389-96
37. De Robertis EM, Sasai Y. 1996. A common plan for dorsoventral patterning in bilateria. Nature 380:37-40
38. Dirksen ML, Jamrich M. 1992. A novel, activin-inducible, blastopore lip-specific gene of Xenopus laevis contains a. fork head DNA-binding domain. Genes Dev. 6:599-608
39. Dohrmann CE, Hemmati-Brivanlou A, Thomsen GH, Fields A, Woolf TM, et al. 1993. Expression of activin mRNA during early development in Xenopus laevis. Dev. Biol. 157:474-83
40. Dohrmann CE, Kessler DS, Melton DA. 1996. Induction of axial mesoderm by Zdvr-1, the zebrafish orthologue of Xenopus Vg1. Dev. Biol. 175:108-17
41. Domingo C, Keller RE. 1995. Induction of notochord cell intercalation behavior and differentiation by progressive signals in the gastrula of Xenopus laevis. Development 121:321-31
42. Dominguez I, Itoh K, Sokol SY. 1995. Role of glycogen synthase kinase 3-beta as a negative regulator of dorsoventral axis formation in Xenopus embryos. Proc. Natl. Acad. Sci. USA 92:8498-502
43. Doniach T, Phillips CR, Gerhart JC. 1992. Planar induction of anteroposterior pattern in the developing central nervous system of Xenopus laevis. Science 257:542-45
44. Donoghue MJ, Patton BL, Sanes JR, Merlie JP. 1992. An axial gradient of transgenemethylation in murine skeletal muscle: genomic imprint of rostrocaudal position. Development 116:1101-12
45. Dosch R, Gawantka V, Delius H, Blumenstock C, Niehrs C. 1997. Mesodermal patterning by antagonizing signals in Xenopus. Ges. Entwicklungsbiol. Abstr. p. 54
46. Dyson S, Gurdon JB. 1997. Activin signalling has a necessary function in Xenopus early development. Curr. Biol. 7:81-84
47. Ekker SC, McGrew LL, Lai CJ, Lee JJ, von Kessler DP, et al. 1995. Distinct expression and shared activities of members of the hedgehog gene family of Xenopus laevis. Development 121:2337-47
48. Elinson RP, Rowning B. 1988. A transient array of parallel microtubules in frog eggs: potential tracks for a cytoplasmic rotation that specifies the dorso-ventral axis. Dev. Biol. 128:185-97
49. Eppert K, Scherer SW, Ozcelik H, Pirone R, Hoodless P, et al. 1996. MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma. Cell 86:543-52
50. Fainsod A, Steinbeisser H, De Robertis EM. 1994. On the function of BMP-4 in pattern-ing the marginal zone of the Xenopus embryo. EMBO J. 13:5015-25
51. Ferguson EL, Anderson KV. 1992. Localized enhancement and repression of the activity of the TGF-β family member, decapentaplegic, is necessary for dorsal-ventral pattern formation in the Drosophila embryo. Development 114:583-97
52. Frank D, Harland RM. 1991. Transient expression of XMyoD in non-somitic mesoderm of Xenopus gastrulae. Development 113:1387-94
53. Friesel R, Dawid IB. 1991. cDNA cloning and developmental expression of fibroblast growth factor receptors from Xenopus laevis. Mol. Cell. Biol. 11:2481-88
54. Fujisue M, Kobayakawa Y, Yamana K. 1993. Occurrence of dorsal axis-inducing activity around the vegetal pole of an uncleaved Xenopus egg and displacement to the equatorial region by cortical rotation. Development 118:163-70
55. Funayama N, Fagotto F, McCrea P, Gumbiner BM. 1995. Embryonic axis induction by the armadillo repeat domain of β-catenin: Evidence for intracellular signaling. J. Cell Biol. 128:959-68
56. Gallagher BC, Hainski AM, Moody SA. 1991. Autonomous differentiation of dorsal axial structures from an animal cap cleavage stage blastomere. Development 112:1103-14
57. Gamer LW, Wright CV. 1995. Autonomous endodermal determination in Xenopus: regulation of expression of the pancreatic gene X1-Hbox 8. Dev. Biol. 171:240-51
58. Gawantka V, Delius H, Hirschfeld K, Blumenstock C, Niehrs C. 1995. Antagonizing the Spemann organizer: role of the homeobox gene Xvent-1. EMBO J. 14:6268-79
59. Gerhart J, Danilchik M, Doniach T, Roberts S, Rowning B, et al. 1989. Cortical rotation of the Xenopus egg: consequences for the anteroposterior pattern of embryonic dorsal development. Development 107:37-51
60. Gerhart J, Doniach T, Stewart R. 1991. Organizing the Xenopus organizer. In Gastrulation: Movements, Patterns, and Molecules, ed. RE Keller, WJ Clark, F Griffin, pp. 57-77. New York: Plenum
61. Gimlich RL. 1986. Acquisition of developmental autonomy in the equatorial region of the Xenopus embryo. Dev. Biol. 115:340-52
62. Gimlich RL, Cooke J. 1983. Cell lineage and the induction of second nervous systems in amphibian development. Nature 306:471-73
63. Gimlich RL, Gerhart JC. 1984. Early cellular interactions promote embryonic axis formation in Xenopus laevis. Dev. Biol. 104:117-30
64. Godsave SF, Slack JM. 1991. Single cell analysis of mesoderm formation in the Xenopus embryo. Development 111:523-30
65. Grabowski CT. 1956. The effects of the excision of Hensen's node on the early development of the chick embryo. J. Exp. Zool. 133:301-44
66. Graff JM, Bansal A, Melton DA. 1996. Xenopus Mad proteins transduce distinct subsets of signals for the TGF beta superfamily. Cell 85:479-87
67. Graff JM, Thies RS, Song JJ, Celeste AJ, Melton DA. 1994. Studies with a Xenopus BMP receptor suggest that ventral mesoderm-inducing signals overide dorsal signals in vivo. Cell 79:169-79
68. Green JBA, Cook TL, Smith JC, Grainger RM. 1997. Anteroposterior neural tissue specification by activin-induced mesoderm. Proc. Natl. Acad. Sci. USA. In press
69. Green JBA, Howes G, Symes K, Cooke J, Smith JC. 1990. The biological effects of XTC-MIF: quantitative comparison with Xenopus bFGF. Development 108:173-83
70. Green JBA, New HV, Smith JC. 1992. Responses of embryonic Xenopus cells to activin and FGF are separated by multiple dose thresholds and correspond to distinct axes of the mesoderm. Cell 71:731-39
71. Green JBA, Smith JC, Gerhart JC. 1994. Slow emergence of a multithreshold response to activin requires cell-contact-dependent sharpening but not prepattern. Development 120:2271-78
72. Grunz H, Tacke L. 1989. Neural differentiation of Xenopus laevis ectoderm takes place after disaggregation and delayed reaggregation without inducer. Cell Differ. Dev. 28:211-17
73. Guger KA, Gumbiner BM. 1995. β-catenin has Wnt-like activity and mimics the Nieuwkoop signaling center in Xenopus dorsal-ventral patterning. Dev. Biol. 172:115-25
74. Gurdon JB, Fairman S, Mohun TJ, Brennan S. 1985. Activation of muscle-specific actin genes in Xenopus development by an inducer between animal and vegetal cells of a blastula. Cell 41:913-22
75. Gurdon JB, Harger P, Mitchell A, Lemaire P. 1994. Activin signalling and response to a morphogen gradient. Nature 371:487-500
76. Gurdon JB, Mitchell A, Mahony D. 1995. Direct and continuous assessment by cells of their position in a morphogen gradient. Nature 376:520-21
77. Gurdon JB, Mitchell A, Ryan K. 1996. An experimental system for analyzing response to a morphogen gradient. Proc. Natl. Acad. Sci. USA 93:9334-38
78. Halpern ME, Ho RK, Walker C, Kimmel CB. 1993. Induction of muscle pioneers and floor plate is distinguished by the zebrafish no tail mutation. Cell 75:99-111
79. Hamburger V. 1988. The Heritage of Experi-mental Embryology: Hans Spemann and the Organizer, New York: Oxford Univ. Press
80. Hansen CS, Marion CD, Steele K, George S, Smith WC. 1997. Direct neural induction and selective inhibition of mesoderm and epidermis inducers by Xnr3. Development 124:483-92
81. Harger PL, Gurdon JB. 1996. Mesoderm induction and morphogen gradients. Semin. Cell Dev. Biol. 7:87-93
82. Harland RM. 1994. Neural induction in Xenopus. Curr. Opin. Genet. Dev. 4:543-49
83. Harland RM. 1996. Neural induction in Xenopus. In Molecular and Cellular Approaches to Neural Development, ed. WM Cowan, TM Jessell, SL Zipursky. Oxford: Oxford Univ. Press. In press
84. Hawley SH, Wunnenberg-Stapleton K, Hashimoto C, Laurent MN, Watabe T, et al. 1995. Disruption of BMP signals in embryonic Xenopus ectoderm leads to direct neural induction. Genes Dev. 9:2923-35
85. He X, Saint-Jennet J-P, Woodgett JR, Varmus HE, Dawid IB. 1995. Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryos. Nature 374:617-22
86. Heasman J, Crawford A, Goldstone K, Garner-Hamrick P, Gumbiner B, et al. 1994. Over-expression of cadherins and underexpression of β-catenin inhibit dorsal mesoderm induction in early Xenopus embryos. Cell 79:791-803
87. Hemmati-Brivanlou A, de la Torre JR, Holt C, Harland RM. 1991. Cephalic expression and molecular characterization of Xenopus En-2. Development 111:715-24
88. Hemmati-Brivanlou A, Kelly OG, Melton DA. 1994. Follistatin, an antagonist of activin, is expressed in the Spemann organizer and displays direct neuralizing activity. Cell 77:283-95
89. Hemmati-Brivanlou A, Melton DA. 1992. A truncated activin receptor inhibits mesoderm induction and formation of axial structures in Xenopus embryos. Nature 359:609-14
90. Hemmati-Brivanlou A, Stewart RM, Harland RM. 1990. Region-specific neural induction of an engrailed protein by anterior notochord in Xenopus. Science 250:800-2
91. Hemmati-Brivanlou A, Thomsen GH. 1995. Ventral mesodermal patterning in Xenopus embryos: expression patterns and activities of BMP-2 and BMP-4. Dev. Genet. 17:78-89
92. Henry GL, Brivanlou IH, Kessler DS, Hemmati-Brivanlou A, Melton DA. 1996. TGF-β signals and a pattern in Xenopus laevis endodermal development. Development 122:1007-15
93. Holley SA, Neul JL, Attisano L, Wrana JL, Sasai Y, et al. 1996. The Xenopus dorsalizing factor noggin ventralizes Drosophila em-bryos by preventing DPP from activating its receptor. Cell 86:607-17
94. Holowacz T, Elinson RP. 1995. Properties of the dorsal activity found in the vegetal cortical cytoplasm of Xenopus eggs. Development 121:2789-98
95. Holtfreter J. 1947. Neural induction in explants which have passed through sublethal cytolysis. J. Exp. Zool. 106:197-222
96. Holtfreter J. 1951. Some aspects of embryonic induction. Growth (Suppl.) 3:117-52
97. Holtfreter J, Hamburger V. 1955. Embryogenesis: progressive differentiation - amphibians. In Analysis of Development, ed. BH Willier, PA Weiss, V Hamburger, pp. 230-96. New York: Haffner. Reprinted 1971
98. Hoodless PA, Haerry T, Abdollah S, Stapleton M, O'Connor MB, et al. 1996. MADR1, a MAD-related protein that functions in BMP2 signaling pathways. Cell 85:489-500
99. Hoppler S, Brown JD, Moon RT. 1996. Expression of a dominant-negative Wnt blocks induction of MyoD in Xenopus embryos. Genes Dev. 10:2805-17
100. Hopwood ND, Pluck A, Gurdon JB. 1989. MyoD expression in the forming somites is an early response to mesoderm induction in Xenopus embryos. EMBO J. 8:3409-17
101. Houliston E, Elinson RP. 1991. Patterns of microtubule polymerization relating to cortical rotation in Xenopus laevis eggs. Development 112:107-18
102. Isaacs HV, Pownall ME, Slack JMW. 1994. eFGF regulates Xbra expression during Xenopus gastrulation. EMBO J. 13:4469-81
103. Isaacs HV, Pownall ME, Slack JMW. 1995. eFGF is expressed in the dorsal midline of Xenopus laevis. Int. J. Dev. Biol. 39:575-79
104. Isaacs HV, Tannahill D, Slack JM. 1992. Expression of a novel FGF in the Xenopus embryo. A new candidate inducing factor for mesoderm formation and anteroposterior specification. Development 114:711-20
105. Jones CM, Armes N, Smith JC. 1996a. Signalling by TGF-β family members - short range effects of Xnr-2 and Bmp-4 contrast with the long-range effects of activin. Curr. Biol. 6:1468-75
106. Jones CM, Dale L, Hogan BL, Wright CV, Smith JC. 1996b. Bone morphogenetic protein-4 (BMP-4) acts during gastrula stages to cause ventralization of Xenopus embryos. Development 122:1545-54
107. Jones CM, Kuehn MR, Hogan BL, Smith JC, Wright CV. 1995. Nodal-related signals induce axial mesoderm and dorsalize mesoderm during gastrulation. Development 121:3651-62
108. Jones CM, Lyons KM, Lapan PM, Wright CV, Hogan BL. 1992. DVR-4 (bone morphogenetic protein-4) as a posterior-ventralizing factor in Xenopus mesoderm induction. Development 115:639-47
109. Jones EA, Woodland HR. 1987. The development of animal cap cells in Xenopus: a measure of the start of animal cap competence to form mesoderm. Development 101:557-64
110. Kageura H. 1990. Spatial distribution of the capacity to initiate a second embryo in the 32-cell embryos of Xenopus laevis. Dev. Biol. 142:432-38
111. Kageura H. 1997. Activation of dorsal development by contact between the cortical dorsal determinant and the equatorial core cytoplasm in eggs of Xenopus laevis. Development. In press
112. Kaneda T. 1981. Studies on the formation and state of determination of the trunk organizer in the newt, Cynops pyrrhogaster. III. Tangential induction in the dorsal marginal zone. Dev. Growth Differ. 23:553-64
113. Kamovsky A, Klymkowsky MW. 1995. Anterior axis duplication in Xenopus induced by the over-expression of the cadherin-binding protein plakoglobin. Proc. Natl. Acad. Sci. USA 92:4522-26
114. Keller R, Danilchik M. 1988. Regional expression, pattern and timing of convergence and extension during gastrulation of Xenopus laevis. Development 103:193-209
115. Keller R, Hardin J. 1987. Cell behaviour during active cell rearrangement: evidence and speculations. J. Cell Sci. Suppl. 8:369-93
116. Keller R, Shih J, Domingo C. 1992. The patterning and functioning of protrusive activity during convergence and extension of the Xenopus organiser. Dev. Suppl. 81-91
117. Keller R, Shih J, Sater AK, Moreno C. 1992. Planar induction of convergence and extension of the neural plate by the organizer of Xenopus. Dev. Dyn. 193:218-34
118. Keller RE. 1975. Vital dye mapping of the gastrula and neurula of Xenopus laevis. I. Prospective areas and morphogenetic movements of the superficial layer. Dev. Biol. 42:222-41
119. Keller RE. 1976. Vital dye mapping of the gastrula and neurula of Xenopus laevis. II. Prospective areas and morphogenetic movements of the deep layer. Dev. Biol. 51:118-37
120. Kengaku M, Okamoto H. 1995. bFGF as a possible morphogen for the anteroposterior axis of the central nervous system in Xenopus. Development 121:3121-30
121. Kessler DS, Melton DA. 1994. Vertebrate embryonic induction: mesodermal and neural patterning. Science 266:596-604
122. Kessler DS, Melton DA. 1995. Induction of dorsal mesoderm by soluble, mature Vg1 protein. Development 121:2155-64
123. Kimelman D, Abraham JA, Haaparanta T, Palisi TM, Kirschner MW. 1988. The presence of fibroblast growth factor in the frog egg: its role as a natural mesoderm inducer. Science 242:1053-56
124. Kimelman D, Christian JL, Moon RT. 1992. Synergistic principles of development: overlapping patterning systems in Xenopus mesoderm induction. Development 116:1-9
125. Kimelman D, Kirschner M. 1987. Synergistic induction of mesoderm by FGF and TGF-β and the identification of a messenger RNA coding for FGF in the early Xenopus embryo. Cell 51:869-78
126. Kinoshita N, Minshull J, Kirschner MW. 1995. The identification of two novel ligands of the FGF receptor by a yeast screening method and their activity in Xenopus development. Cell 83:621-30
127. Klein PS, Melton DA, Schneider S, Steinbeisser H, Warga RM, et al. 1996. A molecular mechanism for the effect of lithium on development. Proc. Natl. Acad. Sci. USA 93:8455-59
128. Knecht AK, Good PJ, Dawid IB, Harland RM. 1995. Dorsal-ventral patterning and differentiation of noggin-induced neural tissue in the absence of mesoderm. Development 121:1927-35
129. Koster M, Plessow S, Clement JH, Lorenz A, Tiedemann H, et al. 1991. Bone morphogenetic protein 4 (BMP-4), a member of the TGF-β family, in early embryos of Xenopus laevis: analysis of mesoderm inducing activity. Mech. Dev. 33:191-99
130. Kroll KL, Amaya E. 1996. Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation. Development 122:3173-83
131. Ku M, Melton DA. 1993. Xwnt-11: a maternally expressed Xenopus wnt gene. Development 119:1161-73
132. LaBonne C, Burke B, Whitman M. 1995. Role of MAP kinase in mesoderm induction and axial patterning during Xenopus development. Development 121:1475-86
133. LaBonne C, Whitman M. 1994. Mesoderm induction by activin requires FGF-mediated intracellular signals. Development 120:463-72
134. LaBonne C, Whitman M. 1997. Localization of MAP kinase activity in early Xenopus embryos: Implications for endogenous FGF signaling. Dev. Biol. 183:9-20
135. Lagna G, Hata A, Hemmati-Brivanlou A, Massagué J. 1996. Partnership between DPC4 and SMAD proteins in TGF-β signalling pathways. Nature 383:832-36
136. Lamb TM, Harland RM. 1995. Fibroblast growth factor is a direct neural inducer, which combined with noggin generates anterior-posterior neural pattern. Development 121:3627-36
137. Lamb TM, Knecht AK, Smith WC, Stachel SE, Economides AN, et al. 1993. Neural induction by the secreted polypeptide noggin. Science 262:713-18
138. Lane MC, Keller RE. 1997. Microtubule disruption reveals that Spemann's organizer is subdivided into two domains by a vegetal alignment zone. Development 124:895-906
139. Larabell CA, Torres M, Rowning BA, Yost C, Miller JR, et al. 1997. Establishment of the dorso-ventral axis in Xenopus is presaged by early asymmetries in β-catenin that are modulated by the Wnt signaling pathway. J. Cell Biol. 136:1123-36
140. Lemaire P, Garret N, Gurdon JB. 1995. Expression cloning of siamois, a Xenopus homeobox gene expressed in dorsal vegetal cells of blastulae and able to induce a complete secondary axis. Cell 81:85-94
141. Lemaire P, Gurdon JB. 1994. A role for cytoplasmic determinants in mesoderm patterning: cell-autonomous activation of the goosecoid and Xwnt-8 genes along the dorsoventral axis of early Xenopus embryos. Development 120:1191-99
142. Leyns L, Bouwmeister T, Kim S-H, Piccolo S, De Robertis EM. 1997. Frz-b is a secreted antagonist of Wnt signaling expressed in the Spemann organizer. Cell 88:747-56
143. Liu F, Hata A, Baker JC, Doody J, Carcamo J, et al. 1996. A human Mad protein acting as a BMP-regulated transcriptional activator. Nature 381:620-23
144. London C, Akers R, Phillips C. 1988. Expression of Epi 1, an epidermis-specific marker in Xenopus laevis embryos, is specified prior to gastrulation. Dev. Biol. 129:380-89
145. Lustig KD, Kroll KL, Sun EE, Kirschner MW. 1996. Expression cloning of a Xenopus T-related gene (Xombi) involved in mesodermal patterning and blastopore lip formation. Development 122:4001-12
146. MacNicol AM, Muslin AJ, Williams LT. 1993. Raf-1 kinase is essential for early Xenopus development and mediates the induction of mesoderm by FGF. Cell 73:571-83
147. Massagué J. 1996. TGFbeta signaling: receptors, transducers, and Mad proteins. Cell 85:947-50
148. Mathers PH, Miller A, Doniach T, Dirksen ML, Jamrich M. 1995. Initiation of anterior head-specific gene expression in uncommitted ectoderm of Xenopus laevis by ammonium chloride. Dev. Biol. 171:641-54
149. Matzuk MM, Lu N, Vogel H, Sellheyer K, Roop DR, et al. 1995. Multiple defects and perinatal death in mice deficient in follistatin. Nature 374:360-63
150. McGrew LL, Lai CJ, Moon RT. 1995. Specification of the anteroposterior neural axis through synergistic interaction of the Wnt signaling cascade with noggin and follistatin. Dev. Biol. 172:337-42
151. McMahon JA, Takada T, Zimmerman LB, Shea M, Xi YZ, et al. 1997. Noggin is required for ventralization of the CNS and somite development in the mouse. Development. In press
152. Merriam JM, Rubenstein AB, Klymkowsky MW. 1997. Cytoplasmically anchored plakoglobin induces a Wnt-like phenotype in Xenopus. Dev. Biol. 185:67-81
153. Molenaar M, van de Wetering M, Oosterwegel M, Peterson-Maduro J, Godsave S, et al. 1996. XTcf-3 transcription factor mediates β-catenin-induced axis formation in Xenopus embryos. Cell 86:391-99
154. Moury JD, Jacobson AG. 1990. The origins of neural crest cells in the axolotl. Dev. Biol. 141:243-53
155. Musci TJ, Amaya E, Kirschner MW. 1990. Regulation of the fibroblast growth factor receptor in early Xenopus embryos. Proc. Natl. Acad. Sci. USA 87:8365-69
156. Nascone N, Mercola M. 1995. An inductive role for the endoderm in Xenopus cardiogenesis. Development 121:515-23
157. Niehrs C, Steinbeisser H, De Robertis EM. 1994. Mesodermal patterning by a gradient of the vertebrate homeobox gene goosecoid. Science 263:817-20
158. Nieuwkoop PD. 1969a. The formation of the mesoderm in urodelean amphibians. I. Induction by the endoderm. Wilhelm Roux's Arch. Entwicklungsmech. Org. 162:341-73
159. Nieuwkoop PD. 1969b. The formation of mesoderm in urodelean amphibians. II. The origin of the dorso-ventral polarity of the mesoderm. Wilhelm Roux's Arch. Entwicklungsmech. Org. 163:298-315
160. Nieuwkoop PD, Albers B. 1990. The role of competence in the cranio-caudal segregation of the nervous system. Dev. Growth Differ. 32:23-31
161. Nieuwkoop PD, Florshutz P. 1950. Quelques caracteres speciaux de la gastrulation et de la neurulation de l'oeuf de Xenopus laevis, Daud, et de quelques autres Anoures. Arch. Biol. 61:113-50
162. Nieuwkoop PD, Koster K. 1995. Vertical versus planar induction in early amphibian development. Dev. Growth Differ. 37:653-68
163. Nieuwkoop PD, Ubbels GA. 1972. The formation of mesoderm in urodelean amphibians. IV. Quantitative evidence for the purely "ectodermal" origin of the entire mesoderm and of the pharyngeal endoderm. Roux's Arch. 169:185-99
164. Nübler-Jung K, Arendt D. 1994. Is ventral in insects dorsal in vertebrates? A history of embryological arguments favouring axis inversion in chordate ancestors. Wilhelm Roux's Arch. Dev. Biol. 203:357-66
165. Olson DJ, Christian JL, Moon RT. 1991. Effect of Wnt-1 and related proteins on gap junctional communication in Xenopus embryos. Science 252:1173-76
166. Onichtchouk D, Gawantka V, Dosch R, Delius H, Hirschfeld K, et al. 1996. The Xvent-2 homeobox gene is pan of the BMP-4 signalling pathway controlling dorsoventral pattern of Xenopus mesoderm. Development 122:3045-53
167. Papalopulu N, Kintner C. 1993. Xenopus Distal-less related homeobox genes are expressed in the developing forebrain and are induced by planar signals. Development 117:961-75
168. Piccolo S, Sasai Y, Lu B, De Robertis EM. 1996. Dorsoventral patterning in Xenopus: inhibition of ventral signals by direct binding of chordin to BMP-4. Cell 86:589-98
169. Pierce SB, Kimelman D. 1995. Regulation of Spemann organizer formation by the intracellular kinase Xgsk-3. Development 121:755-65
170. Pourquie O, Fan CM, Coltey M, Hirsinger E, Watanabe Y, et al. 1996. Lateral and axial signals involved in avian somite patterning: a role for BMP4. Cell 84:461-71
171. Pownall ME, Tucker AS, Slack JM, Isaacs HV. 1996. eFGF, Xcad3 and Hox genes form a molecular pathway that establishes the anteroposterior axis in Xenopus. Development 122:3881-92
172. Psychoyos D, Stern CD. 1996. Restoration of the organizer after radical ablation of Hensen's node and the anterior primitive streak in the chick embryo. Development 122:3263-73
173. Re'em-Kalma Y, Lamb T, Frank D. 1995. Competition between noggin and bone morphogenetic protein 4 activities may regulate dorsalization during Xenopus development. Proc. Natl. Acad. Sci. USA 92:12141-45
174. Reilly KM, Melton DA. 1996. Short-range signaling by candidate morphogens of the TGF beta family and evidence for a relay mechanism of induction. Cell 86:743-54
175. Rosa F, Roberts ZZ, Danielpour D, Dart LL, Sporn MB, et al. 1988. Mesoderm induction in amphibians: the role of TGF-β 2-like factors. Science 240:1443-48
176. Rowning BA, Wells J, Wu M, Gerhart JC, Moon RT, et al. 1997. Microtubule-mediated transport of organelles and localization of β-catenin to the future dorsal side of Xenopus eggs. Proc. Natl. Acad. Sci. USA 94:1224-29
177. Ruiz i Altaba A, Jessell TM. 1992. Pintallavis, a gene expressed in the organizer and midline cells of frog embryos: involvement in the development of the neural axis. Development 116:81-93
178. Saint-Jeannet JP, Dawid IB. 1994. Vertical versus planar neural induction in Rana pipi-ens embryos. Proc. Natl. Acad. Sci. USA 91:3049-53
179. Sakai M. 1996. The vegetal determinants required for the Spemann organizer move equatorially during the first cell cycle. Development 122:2207-14
180. Sasai Y, De Robertis EM. 1997. Ectodermal patterning in vertebrate embryos. Dev. Biol. 182:5-20
181. Sasai Y, Lu B, Piccolo S, De Robertis EM. 1996. Endoderm induction by the organizer secreted factors chordin and noggin in Xenopus animal caps. EMBO J. 15:4547-55
182. Sasai Y, Lu B, Steinbeisser H, De Robertis EM. 1995. Regulation of neural induction by the Chd and Bmp-4 antagonistic patterning signals in Xenopus. Nature 376:333-36
183. Sasai Y, Lu B, Steinbeisser H, Geissert D, Gont LK, et al. 1994. Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes. Cell 79:779-90
184. Scharf SR, Gerhart JC. 1983. Axis determination in eggs of Xenopus laevis: a critical period before first cleavage, identified by the common effects of cold, pressure and ultraviolet irradiation. Dev. Biol. 99:75-87
185. Schneider S, Steinbeisser H, Warga RM, Hausen P. 1996. β-catenin translocation into nuclei demarcates the dorsalizing centers in frog and fish embryos. Mech. Dev. 57:191-98
186. Schoenwolf GC, Garcia-Martinez V, Dias MS. 1992. Mesoderm movement and fate during avian gastrulation and neurulation. Dev. Dyn. 193:235-48
187. Schulte-Merker S, McMahon A, Hammerschmidt M. 1997. Genetic evidence for an essential function of the chordin gene in the zebrafish organizer. Abstr. Ges. Entwicklungsbiol. p. 13
188. Schulte-Merker S, Smith JC. 1995. Mesoderm formation in response to Brachyury requires FGF signalling. Curr. Biol. 5:62-67
189. Schulte-Merker S, Smith JC, Dale L. 1994. Effects of truncated activin and FGF receptors and of follistatin on the inducing activities of BVg1 and activin: does activin play a role in mesoderm induction? EMBO J. 13:3533-41
190. Selleck MAJ, Bronner-Fraser M. 1995. Origins of the avian neural crest: the role of neural plate-epidermal interactions. Development 121:525-38
191. Shih J, Fraser SE. 1996. Characterizing the zebra fish organizer: microsurgical analysis at the early-shield stage. Development 122:1313-22
192. Shih J, Keller R. 1992a. Cell motility driving mediolateral intercalation in explants of Xenopus laevis. Development 116:901-14
193. Shih J, Keller R. 1992b. The epithelium of the dorsal marginal zone of Xenopus has orga-nizer properties. Development 116:887-99
194. Shih J, Keller R. 1992c. Patterns of cell motility in the organizer and dorsal mesoderm of Xenopus laevis. Development 116:915-30
195. Sive H, Bradley L. 1996. A sticky problem: The Xenopus cement gland as a paradigm for anteroposterior patterning. Dev. Dyn. 205:265-80
196. Sive HL, Hattori K, Weintraub H. 1989. Progressive determination during formation of the anteroposterior axis in Xenopus laevis. Cell 58:171-80
197. Slack JMW. 1994. Inducing factors in Xenopus early embryos. Curr. Biol. 4:116-26
198. Slack JMW, Darlington BG, Heath JK, Godsave SF. 1987. Mesoderm induction in early Xenopus embryos by heparin-binding growth factors. Nature 326:197-200
199. Slack JMW, Isaacs HV. 1989. Presence of basic fibroblast growth factor in the early Xenopus embryo. Development 105:147-54
200. Smith JC, Price BM, Green JBA, Weigel D, Herrmann BG. 1991. Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction. Cell 67:79-87
201. Smith JC, Price BM, Van NK, Huylebroeck D. 1990. Identification of a potent Xenopus mesoderm-inducing factor as a homologue of activin A. Nature 345:729-31
202. Smith JC, Slack JMW. 1983. Dorsalization and neural induction: properties of the organizer in Xenopus laevis. J. Embryol. Exp. Morphol. 78:299-317
203. Smith WC, Harland RM. 1991. Injected Xwnt-8 RNA acts early in Xenopus embryos to promote formation of a vegetal dorsalizing center. Cell 67:753-65
204. Smith WC, Harland RM. 1992. Expression cloning of noggin, a new dorsalizing factor localized to the Spemann organizer in Xenopus embryos. Cell 70:829-40
205. Smith WC, Knecht AK, Wu M, Harland RM. 1993. Secreted noggin protein mimics the Spemann organizer in dorsalizing Xenopus mesoderm. Nature 361:547-49
206. Smith WC, McKendry R, Ribisi S, Harland RM. 1995. A nodal-related gene defines a physical and functional domain within the Spemann organizer. Cell 82:37-46
207. Sokol S, Christian JL, Moon RT, Melton DA. 1991. Injected Wnt RNA induces a complete body axis in Xenopus embryos. Cell 67:741-52
208. Sokol S, Melton DA. 1991. Pre-existent pattern in Xenopus animal pole cells revealed by induction with activin. Nature 351:409-11
209. Sokol SY. 1996. Analysis of dishevelled signalling pathways during Xenopus development. Curr. Biol. 6:1456-67
210. Sokol SY, Melton DA. 1992. Interaction of Wnt and activin in dorsal mesoderm induction in Xenopus. Dev. Biol. 154:348-55
211. Spemann H. 1938. Embryonic Development and Induction. New Haven: Yale Univ. Press. 472 pp.
212. Steinbeisser H, De Robertis EM, Ku M, Kessler DS, Melton DA. 1993. Xenopus axis formation: induction of goosecoid by injected Xwnt-8 and activin mRNAs. Development 118:499-507
213. Steinbeisser H, Fainsod A, Niehrs C, Sasai Y, De Robertis EM. 1995. The role of gsc and BMP-4 in dorsal-ventral patterning of the marginal zone in Xenopus: a loss-of-function study using antisense RNA. EMBO J. 14:5230-43
214. Stennard F, Carnac G, Gurdon JB. 1996. The Xenopus T-box gene. Antipodean, encodes a vegetally localised maternal mRNA and can trigger mesoderm formation. Development 122:4179-88
215. Stewart RM, Gerhart JC. 1990. The anterior extent of dorsal development of the Xenopus embryonic axis depends on the quantity of organizer in the late blastula. Development 109:363-72
216. Suzuki A, Thies RS, Yamaji N, Song JJ, Wozney JM, et al. 1994. A truncated bone morphogenetic protein receptor affects dorsal-ventral patterning in the early Xenopus embryo. Proc. Natl. Acad. Sci. USA 91:10255-59
217. Suzuki AS, Mifune Y, Kaneda T. 1984. Germ layer interactions in pattern formation of amphibian mesoderm during primary induction. Dev. Growth Differ. 26:81-94
218. Suzuki A, Shioda N, Ueno N. 1995. Bone morphogenetic protein acts as a ventral mesoderm modifier in early Xenopus embryos. Dev. Growth Differ. 37:581-88
219. Symes K, Smith JC. 1987. Gastrulation movements provide an early marker of mesoderm induction in Xenopus laevis. Development 101:339-50
220. Taira M, Saint-Jeannet J-P, Dawid IB. 1997. Role of the Xlim and Xbra genes in anteroposterior patterning of neural tissue by the head and trunk organizer. Proc. Natl. Acad. Sci. USA 94:895-900
221. Taira M, Jamrich M, Good PJ, Dawid IB. 1992. The LIM domain-containing homeo box gene Xlim-1 is expressed specifically in the organizer region of Xenopus gastrula embryos. Genes Dev. 6:356-66
222. Talbot WS, Trevarrow B, Halpern M, Melby AE, Farr G, et al. 1995. A homeobox gene essential for zebrafish notochord development. Nature 378:150-57
223. Tang TL, Freeman RM Jr. O'Reilly AM, Neel BG, Sokol SY. 1995. The SH2-containing protein-tyrosine phosphatase SH-PTP2 is re-quired upstream of MAP kinase for early Xenopus development. Cell 80:473-83
224. Thomas P, Beddington R. 1996. Anterior primitive endoderm may be responsible for patterning the anterior neural plate in the mouse embryo. Curr. Biol. 11:1487-96
225. Thompson J, Slack JM. 1992. Over-expression of fibroblast growth factors in Xenopus embryos. Mech. Dev. 38:175-82
226. Thomsen G, Woolf T, Whitman M, Sokol S, Vaughan J, et al. 1990. Activins are expressed early in Xenopus embryogenesis and can induce axial mesoderm and anterior structures. Cell 63:485-94
227. Thomsen GH. 1996. Xenopus mothers against decapentaplegic is an embryonic ventralizing agent that acts downstream of the BMP-2/4 receptor. Development 122:2359-66
228. Thomsen GH, Melton DA. 1993. Processed Vg1 protein is an axial mesoderm inducer in Xenopus. Cell 74:433-41
229. Tucker AS, Slack JMW. 1995. Tail bud determination in the vertebrate embryo. Curr. Biol. 5:807-13
230. Uchiyama H, Nakamura T, Komazaki S, Takio K, Asashima M, et al. 1994. Localization of activin and follistatin proteins in the Xenopus oocyte. Biochem. Biophys. Res. Commun. 202:484-89
231. Umbhauer M, Marshall CJ, Mason CS, Old RW, Smith JC. 1995. Mesoderm induction in Xenopus caused by activation of MAP kinase. Nature 376:58-62
232. Varlet I, Collignon J, Robertson EJ. 1997. Nodal expression in the primitive endoderm is required for specification of the anterior axis during mouse gastrulation. Development 124:1033-44
233. Vodicka MA, Gerhart JC. 1995. Blastomere derivation and domains of gene expression in the Spemann organizer of Xenopus laevis. Development 121:3505-18
234. von Dassow G, Schmidt JE, Kimelman D. 1993. Induction of the Xenopus organizer: expression and regulation of Xnot, a novel FGF and activin-regulated homeo box gene. Genes Dev. 7:355-66
235. Wang S, Krinks M, Lin K, Luyten FP, Moos M. 1997. Frzb, a secreted protein expressed in the Spemann organizer, binds and inhibits wnt-8. Cell 88:757-66
236. Watabe T, Kim S, Candia A, Rothbacher U, Hashimoto C, et al. 1995. Molecular mechanisms of Spemann's organizer formation: conserved growth factor synergy between Xenopus and mouse. Genes Dev. 9:3038-50
237. ras in Xenopus mesoderm induction. Nature 357:252-54
238. Wilson PA, Hemmati-Brivanlou A. 1995. Induction of epidermis and inhibition of neural fate by Bmp-4. Nature 376:331-33
239. Wilson PA, Melton DA. 1994. Mesodermal patterning by an inducer gradient depends on secondary cell-cell communication. Curr. Biol. 4:676-86
240. Winklbauer R, Keller RE. 1996. Fibronectin, mesoderm migration, and gastrulation in Xenopus. Dev. Biol. 177:413-26
241. Wylie C, Kofron M, Payne C, Anderson R, Hosobuchi M, et al. 1996. Maternal β-catenin establishes a 'dorsal signal' in early Xenopus embryos. Development 122:2987-96
242. Xu R-H, Kim J, Taira M, Zhan S, Sredni D, Kung H-F. 1995. A dominant negative bone morphogenetic protein 4 receptor causes neuralization in Xenopus ectoderm. Biochem. Biophys. Res. Commun. 212:212-19
243. Yamada T. 1950. Dorsalization of ventral marginal zone of the Triturus gastrula. I. Ammonia-treatment of the medio-ventral marginal zone. Biol. Bull. 47:98-121
244. Yamashita H, ten Dijke P, Huylebroeck D, Sampath TK, Andries M, et al. 1995. Osteogenic protein-1 binds to activin type II receptors and induces certain activin-like effects. J. Cell Biol. 130:217-26
245. Yang-Snyder J, Miller JR, Brown JD, Lai CJ, Moon RT. 1996. A frizzled homolog functions in a vertebrate Wnt signaling pathway. Curr. Biol. 6:1302-6
246. Yost C, Torres M, Miller JR, Huang E, Kimelman D, et al. 1996. The axis-inducing activity, stability, and subcellular distribution of β-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3. Genes Dev. 10:1443-54
247. Youn BW, Malacinski GM. 1981. Axial structure development in ultraviolet-irradiated (notochord-defective) amphibian embryos. Dev. Biol. 83:339-52
248. Yuan S, Darnell DK, Schoenwolf GC. 1995. Mesodermal patterning during avian gastrulation and neurulation: experimental induction of notochord from non-notochordal precursor cells. Dev. Genet. 17:38-54
249. Yuge M, Kobayakawa Y, Fujisue M, Yamana K. 1990. A cytoplasmic determinant for dorsal axis formation in an early embryo of Xenopus laevis. Development 110:1051-56
250. Zhang J, King ML. 1996. Xenopus VegT RNA is localized to the vegetal cortex during oogenesis and encodes a novel T-box transcription factor involved in mesodermal patterning. Development 122:4119-29
251. Zimmerman LB, De Jesús Escobar JM, Harland RM. 1996. The Spemann organizer signal noggin binds and inactivates bone morphogenetic protein 4. Cell 86:599-606
252. Zoltewicz JS, Gerhart JC. 1997. Anteroposterior organization of the Xenopus early gastrula organizer. Dev. Biol. In press