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Microsatellite instability was analyzed as in (20). DNA purified from cultures grown from single cells of the indicated lines was used to amplify microsatellite loci. Four loci were assayed: BAT26, which contains a mononucleotide run; D10S197 and D16S521, which are both dinucleotide repeats; and D7S1794, which contains a trinucleotide repeat. The frequency of instability presented in the text represents the fraction of subclones from each of the lines exhibiting novel alleles at the loci. Patterns of alleles at one of the microsatellite loci (D10S197) in single cell clones isolated from 2774 are presented in Fig. 1.
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We thank D. Carroll, B. Preston, and J. Roth for critically reading this manuscript. Supported by a National Cancer Institute grant (R01-CA-62244) to M.M. and an NIH training grant (T32-CA-09602) to B.R.
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