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Volumn 7, Issue 4, 1997, Pages 474-480

Brachyury and the T-box genes

Author keywords

[No Author keywords available]

Indexed keywords

DNA BINDING PROTEIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR BRACHYURY; UNCLASSIFIED DRUG;

EID: 0030772543     PISSN: 0959437X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-437X(97)80073-1     Document Type: Article
Times cited : (116)

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    • Schmidt C, Wilson V, Stott D, Beddington RSP. T promoter activity in the absence of functional T protein during axis formation and elongation in the mouse. of special interest Dev Biol. 1997; ES cells mutant for Brachyury and which carry a Brachyury promoter-lacZ reporter were introduced into wild-type embryos in a chimera experiment. Brachyury promoter activity occurred in the primitive streak and tailbud in the absence of functional Brachyury protein, arguing against the autoregulatory loop proposed in [44,45], at least for the primitive streak. It remains possible that such a loop occurs in the notochord.
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    • of special interest. An analysis of the mouse Brachyury promoter. Expression in the primitive streak can be driven by 430 bp 5′ of the start of transcription but expression in the notochord could not be achieved even with 8.3 kb of 5' sequence and 5 kb of 3' sequence. These observations lead the authors to suggest that axial and non-axial mesoderm are distinct from the earliest stages of Brachyury expression.
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    • Wilson V, Beddington R. Expression of T protein in the primitive streak is necessary and sufficient for posterior mesoderm movement and somite differentiation. of special interest Dev Biol. 1997; The abnormal migration of the mutant ES cells in [49] can be rescued by expression of wild-type Brachyury under the control of its own promoter, confirming that the aberrant behaviour of these cells is caused by the lack of Brachyury product. Expression of high levels of Brachyury causes cells to leave the streak earlier than those expressing lower levels, reminiscent of the dose-dependent effects of Brachyury in Xenopus [40].
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    • Zhang J, King ML. Xenopus VegT RNA is localized to the vegetal cortex during oogenesis and encodes a novel T-box transcription factor involved in mesodermal patterning. of special interest Development. 122:1996;4119-4129 See annotation [66].
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    • of special interest. This paper, and [63-65], describe the cloning and expression patterns of four Xenopus genes related to mouse Tbx6. Maternal transcripts are localised to the vegetal hemisphere of Xenopus and zygotic expression occurs in the mesoderm. Overexpression of the genes causes the formation of ectopic mesoderm in Xenopus and inhibition of Brat function using a dominant-negative approach [64] interferes with mesoderm formation.
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    • Eomesodermin, a key early gene in Xenopus mesoderm differentiation
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    • Ryan K, Garrett N, Mitchell A, Gurdon JB. Eomesodermin, a key early gene in Xenopus mesoderm differentiation. of special interest Cell. 87:1996;989-1000 Eomesodermin is a T-box gene expressed in the mesoderm of Xenopus from very early stages. There are no maternal transcripts. Expression is higher in dorsal mesoderm than ventral. Misexpression of eomesodermin causes ectopic mesoderm formation and inhibition of eomesodermin interferes with mesoderm formation.
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    • Ryan, K.1    Garrett, N.2    Mitchell, A.3    Gurdon, J.B.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.