Deposition and Characterization of Extended Single-Stranded DNA Molecules on Surfaces

Nano Letters

Volumn 1, Issue 7, 2001, Pages 345-348

  Woolley, Adam T ^a   Kelly, Ryan T ^a  

^a Brigham Young University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0007616320     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl0155476     Document Type: Article

References (36)

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20. 22 During DNA solution translation, a rapidly evaporating (<3 s) film of liquid trailed 1-4 mm behind the moving droplet; after the solution had evaporated the mica was again rinsed with water and dried with compressed air prior to imaging. We did not attempt to study the effect of relative humidity on ssDNA deposition and alignment, but small, day-to-day variations in ambient humidity did not appear to influence our data. Moreover, no significant change in DNA deposition and alignment was observed with poly-L-lys surface incubation times in the range of 2-8 min, or droplet translation velocities varying between 0.2 and 1.0 mm/s, although we did not perform a systematic study of these variables.
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29. 2,5
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32. 3,26 so the droplet trajectory on the surface was marked as a reference for the subsequent alignment step. The mica was rinsed with water, dried with compressed air, treated with 25 μL of 10 ppm poly-L-lysine, rinsed again with water, and dried with compressed air. A 1 μL droplet of ΦX174 ssDNA in TE buffer was then moved across the surface as described above, except that the direction of translation was chosen to align ΦX174 perpendicular to λ. Last, the mica was rinsed with water and dried with compressed air prior to imaging.
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