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Our atomic force microscope is home-constructed. All cantilevers used for this study are sharpened microlevers from Park Scientific Instrument with a force constant of 0.1 N/m.
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Lysozyme (LYZ, from hen egg, 95% purity) was obtained from Sigma Chemical Co., St. Louis, MO.
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7244241292
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note
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The polyclonal, rabbit IgG (purity > 95%) was obtained from Sigma Biochemicals Co., St. Louis, MO. The IgG was diluted to the desired concentration with a solution containing 20 mM HEPES and 150 mM KCl (final pH 6.5 ± 0.5).
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42
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85085632172
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note
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2OH, respectively, with pyridinium dichromate (PDC).
-
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44
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7244250704
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note
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The amino acid sequence of IgG was obtained from the protein data bank at BNL. The PDB ID code is 1IGT.
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45
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7244246174
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note
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The amino acid sequence for LYZ was obtained from the SWISS-PROT protein data bank, ID P00698.
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