Essential Role of Histidine 20 in the Catalytic Mechanism of Escherichia coli Peptidyl-tRNA Hydrolase

Biochemistry

Volumn 43, Issue 15, 2004, Pages 4583-4591

  Goodall, Jonathan J ^a   Chen, Guo Jun ^a,b   Page, Malcolm G P ^a  

^a BASILEA PHARMACEUTICA INTERNATIONAL LTD   (Switzerland)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; ESCHERICHIA COLI; FLUORESCENCE; HYDROLYSIS; MUTAGENESIS; POLYPEPTIDES; RNA; STOICHIOMETRY;

RIBOSOMES; SITE DIRECTED MUTAGENESIS; STOPPED FLOW KINETIC MEASUREMENT;

ENZYMES;

ASPARAGINE; BACTERIAL ENZYME; DIETHYL PYROCARBONATE; HISTIDINE; HYDROLASE; PEPTIDE TRANSFER RIBONUCLEIC ACID HYDROLASE; TRANSFER RNA; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CATALYST; CHEMICAL MODIFICATION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME MODIFICATION; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; FLUORESCENCE; HYDROLYSIS; NONHUMAN; PKA; PRIORITY JOURNAL; PROTEIN BINDING; REACTION ANALYSIS; RIBOSOME; SITE DIRECTED MUTAGENESIS; STOICHIOMETRY;

ASPARAGINE; BINDING SITES; CARBOXYLIC ESTER HYDROLASES; CATALYSIS; DIETHYL PYROCARBONATE; ESCHERICHIA COLI PROTEINS; FLUORESCENT DYES; FLUOROMETRY; HISTIDINE; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; HYDROXYLAMINE; KINETICS; MUTAGENESIS, SITE-DIRECTED; PHOSPHORYLCHOLINE; POINT MUTATION; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 9744223828     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0302200     Document Type: Article

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