Development of a fluorescence polarization assay for peptidyl-tRNA hydrolase

Analytical Biochemistry

Volumn 306, Issue 1, 2002, Pages 8-16

  Bonin, Paul D ^a   Erickson, Laurence A ^a  

^a PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE; HYDROLASES; HYDROLYSIS;

ASSAY SYSTEM; BACTERIAL CELL LYSATE; FIRST-ORDER MODELS; FLUORESCENCE POLARIZATION; HYDROLYZING ACTIVITY; LABELED SUBSTRATES; MEASUREMENTS OF; PREMATURE TERMINATION;

POLARIZATION;

HYDROLASE; PEPTIDE; TRANSFER RNA; 4,4 DIFLUORO 4 BORA 3A,4A DIAZA S INDACENE; 4,4-DIFLUORO-4-BORA-3A,4A-DIAZA-S-INDACENE; BORON DERIVATIVE; FLUORESCEIN; LYSINE TRANSFER RNA LIGASE;

ANALYTIC METHOD; ARTICLE; CELL LYSATE; DISSOCIATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; FLUORESCENCE POLARIZATION; HYDROLYSIS; PRIORITY JOURNAL; RNA TRANSLATION; ACETYLATION; ENZYMOLOGY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KINETICS; METABOLISM; METHODOLOGY; STAPHYLOCOCCUS AUREUS;

BACTERIA (MICROORGANISMS);

ACETYLATION; BORON COMPOUNDS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FLUORESCEIN; FLUORESCENCE POLARIZATION; KINETICS; LYSINE-TRNA LIGASE; STAPHYLOCOCCUS AUREUS;

EID: 0036629191     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2002.5700     Document Type: Article

References (31)

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