Expression of the C-terminus of HIV-1 reverse transcriptase p66 and p51 subunits as a single polypeptide with RNase H activity

Protein Engineering, Design and Selection

Volumn 17, Issue 7, 2004, Pages 581-587

  Zúñiga, Roberto ^a   Sengupta, Sonali ^b   Snyder, Christine ^b   Leon, Oscar ^a   Roth, Monica J ^b  

^a UNIVERSITY OF CHILE   (Chile)

^b RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

Author keywords

C terminus HIV 1 reverse transcriptase; p66 and p51 subunits; RNase H activity; Single polypeptide

Indexed keywords

DIMERS; ESCHERICHIA COLI;

HIV; LYSATES; RNASE; TRANSFER REACTIONS;

POLYPEPTIDES;

MANGANESE; PROTEIN SUBUNIT; RIBONUCLEASE H; RNA DIRECTED DNA POLYMERASE; TRANSFER RNA;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL LYSATE; ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS; MOLECULAR MODEL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; WESTERN BLOTTING;

BLOTTING, WESTERN; CHROMATOGRAPHY, GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HIV-1 REVERSE TRANSCRIPTASE; MANGANESE; MODELS, MOLECULAR; PEPTIDES; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; RIBONUCLEASE H, CALF THYMUS; RNA, TRANSFER; SEPHAROSE; SOFTWARE; UREA;

ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

EID: 9644302663     PISSN: 17410126     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/gzh071     Document Type: Article

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