Guanidinium chloride denaturation of the dimeric Bacillus licheniformis BlaI repressor highlights an independent domain unfolding pathway

Biochemical Journal

Volumn 384, Issue 1, 2004, Pages 179-190

  Vreuls, Christelle ^a   Filée, Patrice ^a   Van Melckebeke, Hélène ^b   Aerts, Tony ^c   De Deyn, Peter ^c   Llabrès, Gabriel ^a   Matagne, André ^a   Simorre, Jean Pierre ^b   Frère, Jean Marie ^a   Joris, Bernard ^a  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^c UNIVERSITY OF ANTWERP   (Belgium)

Author keywords

lactamase induction; BlaI Bacillus licheniformis; Domain structure; Folding intermediate; Guanidinium chloride; Protein folding

Indexed keywords

BACTERIA; CENTRIFUGATION; COLUMN CHROMATOGRAPHY; CROSSLINKING; ENZYMES; FLUORESCENCE; GENES; MONOMERS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SIZE EXCLUSION CHROMATOGRAPHY;

DENATURATION; PROTEOLYSIS; STRUCTURAL DOMAINS; ULTRACENTRIFUGATION;

DIMERS;

8 ANILINO 1 NAPHTHALENESULFONIC ACID; BETA LACTAMASE; GUANIDINE; PAPAIN; PROTEIN BLAI; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS LICHENIFORMIS; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; CROSS LINKING; FLUORESCENCE; GEL PERMEATION CHROMATOGRAPHY; GENETIC TRANSCRIPTION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN INDUCTION; REPRESSOR GENE; ULTRACENTRIFUGATION;

ANILINO NAPHTHALENESULFONATES; BACILLUS; BACTERIAL PROTEINS; CENTRIFUGATION, ISOPYCNIC; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; CROSS-LINKING REAGENTS; DNA, BACTERIAL; FLUORESCENCE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GUANIDINE; MOLECULAR PROBES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PEPTIDES; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; REPRESSOR PROTEINS; SUCCINIMIDES;

BACILLUS LICHENIFORMIS; BACTERIA (MICROORGANISMS); POSIBACTERIA; PROKARYOTA;

EID: 9444225485     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20040658     Document Type: Article

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