Transferrin-Binding Protein B of Neisseria meningitidis: Sequence-Based Identification of the Transferrin-Binding Site Confirmed by Site-Directed Mutagenesis

Journal of Bacteriology

Volumn 186, Issue 3, 2004, Pages 850-857

  Renauld Mongénie, Geneviève ^a   Lins, Laurence ^b   Krell, Tino ^a   Laffly, Laure ^a   Mignon, Michèle ^a   Dupuy, Monique ^a   Delrue, Rose May ^a,c   Guinet Morlot, Françoise ^a   Brasseur, Robert ^b   Lissolo, Ling ^a  

^a SANOFI PASTEUR   (France)

^b UNIVERSITY OF LIÈGE   (Belgium)

^c UNIVERSITY OF NAMUR   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSFERRIN BINDING PROTEIN B;

ARTICLE; BACTERICIDAL ACTIVITY; CALORIMETRY; GENE MUTATION; GENETIC CONSERVATION; LIGAND BINDING; NEISSERIA MENINGITIDIS; NONHUMAN; OUTER MEMBRANE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN LOCALIZATION; RECEPTOR AFFINITY; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANTIBODIES, BACTERIAL; BINDING SITES; CIRCULAR DICHROISM; MENINGOCOCCAL VACCINES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NEISSERIA MENINGITIDIS; TRANSFERRIN; TRANSFERRIN-BINDING PROTEIN B;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; NEISSERIA; NEISSERIA GONORRHOEAE; NEISSERIA MENINGITIDIS;

EID: 9144274926     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.186.3.850-857.2004     Document Type: Article

References (33)

1. Boulton, I. C., A. R. Gorringe, R. J. Carr, B. Gorinsky, C. L. Joannou, and R. W. Evans. 1997. Characterisation of the meningococcal transferrin-binding protein complex by photon correlation spectroscopy. FEBS Lett. 414:409-413.
2. Caugant, D. A., L. O. Froholm, K. Bovre, E. Holten, C. E. Frasch, L. F. Mocca, W. D. Zollinger, and R. K. Selander. 1986. Intercontinental spread of a genetically distinctive complex of clones of Neisseria menigitidis causing epidemic disease. Proc. Natl. Acad. Sci. USA 83:4927-4931.
3. Combet, C., C. Blanchet, C. Geourjon, and G. Deléage. 2000. NPS@: Network Protein Sequence Analysis. Trends Biochem. Sci. 25:147-150.
4. Cornelissen, C. N., J. E. Anderson, and P. F. Sparling. 1997. Characterization of the diversity and the transferrin-binding domain of gonococcal transferrin-binding protein 2. Infect. Immun. 65:822-828.
5. Danve, B., L. Lissolo, M. Mignon, P. Dumas, S. Colombani, A. B. Schryvers, and M.-J. Quentin-Millet. 1993. Transferrin-binding proteins isolated from Neisseria meningitidis elicit protective and bactericidal antibodies in laboratory animals. Vaccine 11:1214-1220.
6. De Loof, H., M. Rossenau, R. Brasseur, and J. M. Ruysschaert. 1986. Use of hydrophobicity profiles to predict receptor binding domains on apolipoprotein E and the low-density lipoprotein apolipoprotein B-E receptor. Proc. Natl. Acad. Sci. USA 83:2295-2299.
7. Eisenberg, D., R. M. Weiss, and T. C. Terwilliger. 1982. The helical hydrophobic moment: a measure of the amphiphilicity of a helix. Nature 299:371-374.
8. Eisenberg, D., E. Schwarz, M. Komaromy, and R. Wall. 1984. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J. Mol. Biol. 179:125-142.
9. Fuller, C. A., R. Yu, S. W. Irwin, and A. B. Schryvers. 1998. Biochemical evidence for a conserved interaction between bacterial transferrin-binding protein A and transferrin-binding protein B. Microb. Pathog. 24:75-87.
10. Gallet, X., B. Charloteaux, A. Thomas, and R. Brasseur. 2000. A fast method to predict Protein interaction sites from sequences. J. Mol. Biol. 302:917-926.
11. Gray-Owen, S. D., and A. B. Schryvers. 1996. Bacterial transferrin and lactoferrin receptors. Trends Microbiol. 14:843-850.
12. Holdgate, G. A. 2001. Making cool drugs hot: the use of isothermal titration calorimetry as a tool to study binding energetics. BioTechniques 31:164-184.
13. Krell, T., G. Renauld-Mongenie, M.-C. Nicolai, S. Fraysse, M. Chevalier, Y. Berard, J. Oakhill, R. W. Evans, A. Gorringe, and L. Lissolo. 2003. Insight into the structure and function of the transferrin receptor from Neisseria meningitidis using microcalorimetric techniques. J. Biol. Chem. 278:14712-14722.
14. Legrain, M., V. Mazariu, S. W. Irwin, B. Bouchon, M.-J. Quentin-Millet, E. Jacobs, and A. B. Schryvers. 1993. Cloning and characterization of Neisseria meningitidis genes encoding the transferrin-binding proteins Tbp1 and Tbp2. Gene 130:73-80.
15. Legrain, M., A. Findeli, D. Villeval, M.-J. Quentin-Millet, and E. Jacobs. 1996. Molecular characterization of hybrid Tbp2 proteins from Neisseria meningitidis. Mol. Microbiol. 19:159-169.
16. Legrain, M., B. Rokbi, D. Villeval, and E. Jacobs. 1998. Characterization of genetic exchanges between various highly divergent tbpBs having occurred in Neisseria meningitidis. Gene 208:51-59.
17. Lissolo, L., G. Maitre-Wilmotte, P. Dumas, S. Colombani, A. B. Schryvers, and M.-J. Quentin-Millet. 1995. Evaluation of transferrin-binding protein 2 within the transferrin-binding protein complex as a potential antigen for future meningococcal vaccines. Infect. Immun. 63:884-890.
18. Marrec-Fairley, M., A. Piette, X. Gallet, R. Brasseur, H. Hara, C. Faipont, J.-M. Ghysen and J.-M. Nguyen-Distèche. 2000. Differential functionalities of amphiphilic peptide segments of the cell-septation penicillin-binding protein 3 of Escherichia coli. Mol. Microbiol. 37:1019-1031.
19. Mazarin, V., B. Rokbi, and M.-J. Quentin-Millet. 1995. Diversity of the transferrin-binding protein Tbp2 of Neisseria meningitidis. Gene 158:145-146.
20. Nairn, J., N. C. Price, S. M. Kelly, D. Rigden, L. A. Fothergill-Gilmore, and T. Krell. 1996. Phosphoglycerate mutase from Schizosccharomyces pombe: development of an expression system and characterization of three histidine mutants of the enzyme. Biochim. Biophys. Acta 1296:69-75.
21. Renauld-Mongenie, G., D. Poncet, L. von Olleschik-Elbheim, T. Cournez, M. Mignon, M. A. Schmidt, and M.-J. Quentin-Millet. 1997. Identification of human transferrin-binding sites within meningococcal transferrin-binding protein B. J. Bacteriol. 179:6400-6407.
22. Renauld-Mongénie, G., M. Latour, D. Poncet, S. Naville, and M.-J. Quentin-Millet. 1998. Both the full-length and the N-terminal domain of the meningococcal transferrin-binding protein B discriminate between human iron-loaded and apo-transferrin. FEMS Microbiol. Lett. 169:171-177.
23. Retzer, M. D., R. Yu, and A. B. Schryvers. 1999. Identification of sequences in human transferrin that bind to the bacterial receptor protein, transferrin-binding protein B. Mol. Microbiol. 32:111-121.
24. Rokbi, B., G. Renauld-Mongenie, M. Mignon, B. Danve, D. Poncet, C. Chabanel, D. A. Caugant, and M. J. Quentin-Millet. 2000. Allelic diversity of the two transferrin binding protein B gene isotypes among a collection of Neisseria meningitidis strains representative of serogroup B disease: implication for the composition of a recombinant TbpB-based vaccine. Infect. Immun. 68:4938-4947.
25. Rokbi, B., M. Mignon, G. Maitre-Wilmott, L. Lissolo, B. Danve, D. A. Caugant, and M. J. Quentin-Millet. 1997. Evaluation of recombinant transferrin-binding protein B variants from Neisseria meningitidis for their ability to induce cross-reactive and bactericidal antibodies against a genetically diverse collection of serogroup B strains. Infect. Immun. 65:55-63.
26. Rokbi, B., V. Mazarin, G. Maitre-Willmotte, and M. J. Quentin-Millet. 1993. Identification of two major families of transferrin receptors among Neisseria meningitidis strains based on antigenic and genomic features. FEMS Microbiol. Lett. 110:51-58.
27. Ronpirin, C., A. E. Jerse, and C. N. Cornelisson. 2001. Gonococcal genes encoding transferrin-binding proteins A and B are arranged in a bicistronic operon but are subject to differential expression. Infect. Immun. 69:6336-6347.
28. Schryvers, A. B., and I. I. Stojilkovie. 1999. Iron acquisition systems in the pathogenic Neisseria. Mol. Microbiol. 32:1117-1123.
29. Schryvers, A. B., and L. J. Morris. 1988. Identification and characterization of the transferrin receptor from Neisseria meningitidis. Mol. Microbiol. 2:281-288.
30. Sims, K. L., and A. B. Schryvers. 2003. Peptide-peptide interactions between human transferrin and transferrin-binding protein B from Moraxella catarrhalis. J. Bacteriol. 185:2603-2610.
31. Taylor, J. W., and E. T. Kaiser. 1987. Structure-function analysis of proteins through the design, synthesis, and study of peptide models. Methods Enzymol. 154:473-498.
32. Tettelin, H., N. J., Saunders, J. Heidelberg, A. C. Jeffries, K. E. Nelson, J. A. Eisen, K. A. Ketchum, D. W. Hood, J. F. Peden, R. J. Dodson, W. C. Nelson, M. L. Gwinn, R. DeBoy, J. D. Peterson, E. K. Hickey, D. H. Haft, S. L. Salzberg, O. White, R. D. Fleischmann, B. A. Dougherty, T. Mason, A. Ciecko, D. S. Parksey, E. Blair, H. Cittone, E. B. Clark, M. D. Cotton, T. R. Utterback, H. Khouri, H. Qin, J. Vamathevan, J. Gill, V. Scarlato, V. Masignani, M. Pizza, G. Grandi, L. Sun, H. O. Smith, C. M. Fraser, E. R. Moxon, R. Rappuoli, and J. C. Venter. 2000. Complete genome sequence of Neisseria meningitidis serogroup B strain MC58. Science 287:1809-1815.
33. Vonder Haar, R. A., M. Legrain, H. V. J. Kolbe, and E. Jacobs. 1994. Characterization of a highly structured domain in Tbp2 from Neisseria meningitidis involved in binding to human transferrin. J. Bacteriol. 176:6207-6213.