A new mutation in spo0A with intragenic suppressors in the effector domain

FEMS Microbiology Letters

Volumn 185, Issue 2, 2000, Pages 123-128

  Schmeisser, Falko ^a   Brannigan, James A ^b   Lewis, Richard J ^b   Wilkinson, Anthony J ^b   Youngman, Phil ^c   Barák, Imrich ^a  

^a INSTITUTE OF CHEMISTRY   (Slovakia)

^b UNIVERSITY OF YORK   (United Kingdom)

^c University of Georgia ^*  (United States)

Author keywords

Galactosidase assay; Bacillus subtilis; Mutagenesis; Sigma factor; Sporulation; Suppressor mutation

Indexed keywords

BETA GALACTOSIDASE; MUTANT PROTEIN; SIGMA FACTOR;

ARTICLE; BACILLUS SUBTILIS; DNA SEQUENCE; GENE EXPRESSION; GENETIC TRANSCRIPTION; MUTAGENESIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; SPOROGENESIS;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BACTERIAL PROTEINS; CULTURE MEDIA; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR SEQUENCE DATA; PLASMIDS; PROMOTER REGIONS (GENETICS); SIGMA FACTOR; SPORES, BACTERIAL; SUPPRESSION, GENETIC; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

BACILLUS SUBTILIS; CLOSTRIDIUM;

EID: 0034655874     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00083-5     Document Type: Article

References (24)

1. Stock J.B., Ninfa A.J., Stock A.M. Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol. Rev. 53:1989;450-490.
2. Hoch J.A. The phosphorelay signal transduction pathway in the initiation of Bacillus subtilis sporulation. J. Cell. Biochem. 51:1993;55-61.
3. Ireton K., Rudner D.Z., Siranosian K.J., Grossman A.D. Integration of multiple developmental signals in Bacillus subtilis through the Spo0A transcription factor. Genes Dev. 7:1992;283-294.
4. Lewis R.J., Brannigan J.A., Muchová K., Barák I., Wilkinson A.J. Phosphorylated aspartate in the structure of a response regulator protein. J. Mol. Biol. 294:1999;9-15.
5. Spiegelman, G., Bird, T.H. and Voon, V. (1995) Transcription regulation by the Bacillus subtilis response regulator Spo0A. In: Two-Component Signal Transduction (Hoch, J.A. and Silhavy, T.J., Eds.), pp. 159-179. American Society for Microbiology Press, Washington, DC.
6. Baldus J.M., Buckner C.M., Moran C.P. Jr. Evidence that the transcriptional activator Spo0A interacts with two sigma factors in Bacillus subtilis. Mol. Microbiol. 17:1995;281-290.
7. H required for Spo0A-dependent promoter activity. J. Bacteriol. 180:1998;4987-4990.
8. Buckner C.M., Schyns G., Moran C.P. Jr. A region in the Bacillus subtilis transcription factor Spo0A that is important for spoIIG promoter activation. J. Bacteriol. 180:1998;3578-3583.
9. Hatt J.K., Youngman P. Spo0A mutants of Bacillus subtilis with sigma factor-specific defects in transcription activation. J. Bacteriol. 180:1998;3584-3591.
10. Brown D.P., Ganova-Raeva L., Green B.D., Wilkinson S.R., Young M., Youngman P. Characterization of spo0A homologues in diverse Bacillus and Clostridium species identifies a probable DNA binding domain. Mol. Microbiol. 14:1994;411-426.
11. Perego M., Wu J.-J., Spiegelman G.B., Hoch J.A. Mutational dissociation of the positive and negative regulatory properties of the Spo0A sporulation transcription factor of Bacillus subtilis. Gene. 100:1991;207-212.
12. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.O., Seidmann, J.S., Smith, J. and Struhl, K. (1987) Current Protocols in Molecular Biology. Wiley, New York.
13. Harwood, C.R. and Cutting, S.M. (1990) Molecular Biological Methods for Bacillus. John Wiley and Sons, Chichester.
14. Kunkel T.A., Roberts J.D., Zakour R.A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154:1987;367-382.
15. Backman K., Ptashne M., Gilbert A.W. Construction of plasmids carrying the cI gene of bacteriophage lambda. Proc. Natl. Acad. Sci. USA. 73:1976;4174-4178.
16. Yanish-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 33:1985;103-119.
17. Guzmán P., Westpheling J., Youngman P. Characterisation of the promoter region of the Bacillus subtilis spoIIE operon. J. Bacteriol. 170:1988;1598-1609.
18. A with two promoters in Bacillus subtilis. J. Bacteriol. 173:1991;3282-3290.
19. Zuber P., Losick R. Role of AbrB in Spo0A- and Spo0B-dependent utilization of a sporulation promoter in Bacillus subtilis. J. Bacteriol. 169:1987;2223-2230.
20. Youngman P., Perkins J.B., Losick R. Construction of a cloning site near one end of Tn917 into which foreign DNA may be inserted without affecting transposition in Bacillus subtilis or expression of the transposon-borne erm gene. Plasmid. 12:1984;1-9.
21. Strauch M.A., Webb V., Spiegelman G.B., Hoch J.A. The Spo0A protein of Bacillus subtilis is a repressor of the abrB gene. Proc. Natl. Acad. Sci. USA. 87:1990;1801-1805.
22. Muchová K., Lewis R.J., Brannigan J.A., Offen W., Brown D.P., Barák I., Youngman P., Wilkinson A.J. Crystallization of the regulatory and effector domains of the key sporulation response regulator Spo0A. Acta Crystallogr. D. 55:1999;671-676.
23. Youngman, P. (1990) Use of transposons and integrational vectors for mutagenesis and construction of gene fusions in Bacillus species. In: Molecular Biological Methods for Bacillus (Harwood, C.R. and Cutting, S.M., Eds.), pp. 221-266. John Wiley and Sons, Chichester.
24. Piggot P.J., Curtis C.A.M. Analysis of the regulation of gene expression during Bacillus subtilis sporulation by manipulation of the copy number of spo-lacZ fusions. J. Bacteriol. 87:1987;1260-1266.