Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States

Emerging Infectious Diseases

Volumn 26, Issue 6, 2020, Pages 1266-1273

  Harcourt, Jennifer ^a   Tamin, Azaibi ^a   Lu, Xiaoyan ^a   Kamili, Shifaq ^b   Sakthivel, Senthil K ^b   Murray, Janna ^b   Queen, Krista ^a   Tao, Ying ^a   Paden, Clinton R ^a   Zhang, Jing ^b   Li, Yan ^a   Uehara, Anna ^c   Wang, Haibin ^c   Goldsmith, Cynthia ^a   Bullock, Hannah A ^d   Wang, Lijuan ^b,d   Whitaker, Brett ^a   Lynch, Brian ^b   Gautam, Rashi ^a   Schindewolf, Craig ^e   Lokugamage, Kumari G ^e   Scharton, Dionna ^f   Plante, Jessica A ^f   Mirchandani, Divya ^e   Widen, Steven G ^e   Narayanan, Krishna ^e   Makino, Shinji ^e   Ksiazek, Thomas G ^e,f   Plante, Kenneth S ^f   Weaver, Scott C ^e,f   Lindstrom, Stephen ^a   Tong, Suxiang ^a   Menachery, Vineet D ^e,f   Thornburg, Natalie J ^a   more..

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^b Ihrc Inc   (United States)

^c OAK RIDGE INSTITUTE FOR SCIENCE AND EDUCATION   (United States)

^d Synergy CIS   (United States)

^e UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

^f World Reference Center for Emerging Viruses and Arboviruses   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEOCAPSID PROTEIN; VIRUS ANTIBODY;

A-549 CELL LINE; AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ARTICLE; CELL CULTURE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; CORONAVIRUS DISEASE 2019; CYTOPATHOGENIC EFFECT; ELECTRON MICROSCOPY; GROWTH CURVE; HEK293T CELL LINE; HUH-7 CELL LINE; HUMAN; HUMAN CELL; MORPHOGENESIS; NESTED POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHASE CONTRAST MICROSCOPY; PROTEIN EXPRESSION; REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SEQUENCE; SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2; VERO CCL81 CELL LINE; VERO CELL LINE; VERO E6 CELL LINE; VIRUS ISOLATION; VIRUS MORPHOLOGY; VIRUS RELEASE; VIRUS REPLICATION; VIRUS TITRATION; WESTERN BLOTTING; WHOLE GENOME SEQUENCING; ANIMAL; BETACORONAVIRUS; CELL LINE; CHLOROCEBUS AETHIOPS; CORONAVIRUS INFECTION; GENETICS; ISOLATION AND PURIFICATION; NASOPHARYNX; OROPHARYNX; PANDEMIC; PHYSIOLOGY; VIRAL TROPISM; VIROLOGY; VIRUS GENOME; VIRUS PNEUMONIA; WASHINGTON;

ANIMALS; BETACORONAVIRUS; CELL LINE; CHLOROCEBUS AETHIOPS; CORONAVIRUS INFECTIONS; GENOME, VIRAL; HUMANS; NASOPHARYNX; OROPHARYNX; PANDEMICS; PNEUMONIA, VIRAL; VERO CELLS; VIRAL TROPISM; VIRUS REPLICATION; WASHINGTON;

EID: 85082747770     PISSN: 10806040     EISSN: 10806059     Source Type: Journal    
DOI: 10.3201/EID2606.200516     Document Type: Article

References (24)

1. Holshue ML, De Bolt C, Lindquist S, Lofy KH, Wiesman J, Bruce H, et al.; Washington State 2019-nCoV Case Investigation Team. First case of 2019 novel coronavirus in the United States. N Engl J Med. 2020;382:929-36. https://doi.org/10.1056/NEJMoa2001191
2. Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, et al.; China Novel Coronavirus Investigating and Research Team. A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med. 2020;382:727-33. https://doi.org/ 10.1056/NEJMoa2001017
3. Lu R, Zhao X, Li J, Niu P, Yang B, Wu H, et al. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet. 2020;395:565-74. https://doi.org/10.1016/S0140-6736(20)30251-8
4. Chan JF, Kok KH, Zhu Z, Chu H, To KK, Yuan S, et al. Genomic characterization of the 2019 novel human-pathogenic coronavirus isolated from a patient with atypical pneumonia after visiting Wuhan. Emerg Microbes Infect. 2020;9:221-36. https://doi.org/10.1080/22221751.2020.1719902
5. Li Q, Guan X, Wu P, Wang X, Zhou L, Tong Y, et al. Early transmission dynamics in Wuhan, China, of novel coronavirus-infected pneumonia. N Engl J Med. 2020 Jan 29 [Epub ahead of print]. https://doi.org/10.1056/NEJMoa2001316
6. Chen N, Zhou M, Dong X, Qu J, Gong F, Han Y, et al. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. Lancet. 2020;395:507-13. https://doi.org/ 10.1016/S0140-6736(20)30211-7
7. Chan JF, Yuan S, Kok KH, To KK, Chu H, Yang J, et al. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. Lancet. 2020;395:514-23. https://doi.org/10.1016/S0140-6736(20)30154-9
8. Wan Y, Shang J, Graham R, Baric RS, Li F. Receptor recognition by novel coronavirus from Wuhan: an analysis based on decade-long structural studies of SARS. J Virol. 2020 Jan 29 [Epub ahead of print]. https://doi.org/10.1128/JVI.00127-20
9. Sims AC, Tilton SC, Menachery VD, Gralinski LE, Schäfer A, Matzke MM, et al. Release of severe acute respiratory syndrome coronavirus nuclear import block enhances host transcription in human lung cells. J Virol. 2013;87:3885-902. https://doi.org/10.1128/JVI.02520-12
10. Josset L, Menachery VD, Gralinski LE, Agnihothram S, Sova P, Carter VS, et al. Cell host response to infection with novel human coronavirus EMC predicts potential antivirals and important differences with SARS coronavirus. MBio. 2013;4:e00165-13. https://doi.org/10.1128/mBio.00165-13
11. Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014;30:2114-20. https://doi.org/10.1093/bioinformatics/btu170
12. Simpson JT, Wong K, Jackman SD, Schein JE, Jones SJ, Birol I. ABySS: a parallel assembler for short read sequence data. Genome Res. 2009;19:1117-23. https://doi.org/10.1101/gr.089532.108
13. Li H, Durbin R. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics. 2009;25:1754-60. https://doi.org/10.1093/bioinformatics/btp324
14. Robinson JT, Thorvaldsdóttir H, Winckler W, Guttman M, Lander ES, Getz G, et al. Integrative genomics viewer. Nat Biotechnol. 2011;29:24-6. https://doi.org/10.1038/nbt.1754
15. Das D, Suresh MR. Copious production of SARS-CoV nucleocapsid protein employing codon optimized synthetic gene. J Virol Methods. 2006;137:343-6. https://doi.org/10.1016/j.jviromet.2006.06.029
16. Coutard B, Valle C, de Lamballerie X, Canard B, Seidah NG, Decroly E. The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade. Antiviral Res. 2020;176:104742. https://doi.org/10.1016/j.antiviral.2020.104742
17. Stieneke-Gröber A, Vey M, Angliker H, Shaw E, Thomas G, Roberts C, et al. Influenza virus hemagglutinin with multibasic cleavage site is activated by furin, a subtilisin-like endoprotease. EMBO J. 1992;11:2407-14. https://doi.org/ 10.1002/j.1460-2075.1992.tb05305.x
18. Li W, Moore MJ, Vasilieva N, Sui J, Wong SK, Berne MA, et al. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature. 2003;426:450-4. https://doi.org/10.1038/nature02145
19. Chan JF, Chan KH, Choi GK, To KK, Tse H, Cai JP, et al. Differential cell line susceptibility to the emerging novel human betacoronavirus 2c EMC/2012: implications for disease pathogenesis and clinical manifestation. J Infect Dis. 2013;207:1743-52. https://doi.org/10.1093/infdis/jit123
20. Gillim-Ross L, Taylor J, Scholl DR, Ridenour J, Masters PS, Wentworth DE. Discovery of novel human and animal cells infected by the severe acute respiratory syndrome coronavirus by replication-specific multiplex reverse transcription-PCR. J Clin Microbiol. 2004;42:3196-206. https://doi.org/10.1128/JCM.42.7.3196-3206.2004
21. Kaye M, Druce J, Tran T, Kostecki R, Chibo D, Morris J, et al. SARS-associated coronavirus replication in cell lines. Emerg Infect Dis. 2006;12:128-33. https://doi.org/10.3201/ eid1201.050496
22. Swayne DE, Suarez DL, Spackman E, Tumpey TM, Beck JR, Erdman D, et al. Domestic poultry and SARS coronavirus, southern China. Emerg Infect Dis. 2004;10:914-6. https://doi.org/10.3201/eid1005.030827
23. Scobey T, Yount BL, Sims AC, Donaldson EF, Agnihothram SS, Menachery VD, et al. Reverse genetics with a full-length infectious cDNA of the Middle East respiratory syndrome coronavirus. Proc Natl Acad Sci U S A. 2013;110:16157-62. https://doi.org/10.1073/pnas.1311542110
24. Yount B, Curtis KM, Fritz EA, Hensley LE, Jahrling PB, Prentice E, et al. Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus. Proc Natl Acad Sci U S A. 2003;100:12995-3000. https://doi.org/10.1073/pnas.173558210