메뉴 건너뛰기




Volumn 8, Issue 7, 2019, Pages 1673-1678

An Engineered B. subtilis Inducible Promoter System with over 10 000-Fold Dynamic Range

Author keywords

Bacillus subtilis; inducible promoter system; IPTG; protein expression; T7 RNA polymerase

Indexed keywords

BETA GALACTOSIDASE; MESSENGER RNA; RNA POLYMERASE; T7 RNA POLYMERASE; TISSUE FACTOR PATHWAY INHIBITOR; UNCLASSIFIED DRUG; BACTERIOPHAGE T7 RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE; GREEN FLUORESCENT PROTEIN; VIRAL PROTEIN;

EID: 85069489878     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.8b00469     Document Type: Article
Times cited : (36)

References (38)
  • 1
    • 84937250495 scopus 로고    scopus 로고
    • Chromosomal Arrangement of Phosphorelay Genes Couples Sporulation and DNA Replication
    • Narula, J. et al. (2015) Chromosomal Arrangement of Phosphorelay Genes Couples Sporulation and DNA Replication. Cell 162, 328-337, 10.1016/j.cell.2015.06.012
    • (2015) Cell , vol.162 , pp. 328-337
    • Narula, J.1
  • 2
    • 80054837496 scopus 로고    scopus 로고
    • Stochastic Pulse Regulation in Bacterial Stress Response
    • Locke, J. C. W., Young, J. W., Fontes, M., Jiménez, M. J. H., and Elowitz, M. B. (2011) Stochastic Pulse Regulation in Bacterial Stress Response. Science 334, 366-369, 10.1126/science.1208144
    • (2011) Science , vol.334 , pp. 366-369
    • Locke, J.C.W.1    Young, J.W.2    Fontes, M.3    Jiménez, M.J.H.4    Elowitz, M.B.5
  • 3
    • 84946221763 scopus 로고    scopus 로고
    • Ion channels enable electrical communication in bacterial communities
    • Prindle, A. et al. (2015) Ion channels enable electrical communication in bacterial communities. Nature 527, 59-63, 10.1038/nature15709
    • (2015) Nature , vol.527 , pp. 59-63
    • Prindle, A.1
  • 4
    • 8844278305 scopus 로고    scopus 로고
    • Bacillus subtilis as cell factory for pharmaceutical proteins: A biotechnological approach to optimize the host organism
    • Westers, L., Westers, H., and Quax, W. J. (2004) Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism. Biochim. Biophys. Acta, Mol. Cell Res. 1694, 299-310, 10.1016/j.bbamcr.2004.02.011
    • (2004) Biochim. Biophys. Acta, Mol. Cell Res. , vol.1694 , pp. 299-310
    • Westers, L.1    Westers, H.2    Quax, W.J.3
  • 5
    • 84872143710 scopus 로고    scopus 로고
    • Bacillus subtilis: From soil bacterium to super-secreting cell factory
    • van Dijl, J. and Hecker, M. (2013) Bacillus subtilis: from soil bacterium to super-secreting cell factory. Microb. Cell Fact. 12, 3, 10.1186/1475-2859-12-3
    • (2013) Microb. Cell Fact. , vol.12 , pp. 3
    • Van Dijl, J.1    Hecker, M.2
  • 6
    • 84888599866 scopus 로고    scopus 로고
    • The Bacillus BioBrick Box: Generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis
    • Radeck, J. et al. (2013) The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J. Biol. Eng. 7, 29, 10.1186/1754-1611-7-29
    • (2013) J. Biol. Eng. , vol.7 , pp. 29
    • Radeck, J.1
  • 7
    • 84988380512 scopus 로고    scopus 로고
    • A part toolbox to tune genetic expression in Bacillus subtilis
    • Guiziou, S. et al. (2016) A part toolbox to tune genetic expression in Bacillus subtilis. Nucleic Acids Res. 44, 7495-7508, 10.1093/nar/gkw624
    • (2016) Nucleic Acids Res. , vol.44 , pp. 7495-7508
    • Guiziou, S.1
  • 8
    • 85033369527 scopus 로고    scopus 로고
    • The Bacillus BioBrick Box 2.0: Expanding the genetic toolbox for the standardized work with Bacillus subtilis
    • Popp, P. F., Dotzler, M., Radeck, J., Bartels, J., and Mascher, T. (2017) The Bacillus BioBrick Box 2.0: expanding the genetic toolbox for the standardized work with Bacillus subtilis. Sci. Rep. 7, 15058, 10.1038/s41598-017-15107-z
    • (2017) Sci. Rep. , vol.7 , pp. 15058
    • Popp, P.F.1    Dotzler, M.2    Radeck, J.3    Bartels, J.4    Mascher, T.5
  • 9
    • 0036199577 scopus 로고    scopus 로고
    • Engineering of a Bacillus subtilis Strain with Adjustable Levels of Intracellular Biotin for Secretory Production of Functional Streptavidin
    • Wu, S.-C. and Wong, S.-L. (2002) Engineering of a Bacillus subtilis Strain with Adjustable Levels of Intracellular Biotin for Secretory Production of Functional Streptavidin. Appl. Environ. Microbiol. 68, 1102-1108, 10.1128/AEM.68.3.1102-1108.2002
    • (2002) Appl. Environ. Microbiol. , vol.68 , pp. 1102-1108
    • Wu, S.-C.1    Wong, S.-L.2
  • 10
    • 0002412951 scopus 로고
    • Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis
    • Yansura, D. G. and Henner, D. J. (1984) Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis. Proc. Natl. Acad. Sci. U. S. A. 81, 439-443, 10.1073/pnas.81.2.439
    • (1984) Proc. Natl. Acad. Sci. U. S. A. , vol.81 , pp. 439-443
    • Yansura, D.G.1    Henner, D.J.2
  • 11
    • 0030605248 scopus 로고    scopus 로고
    • A xylose-inducible Bacillus subtilis integration vector and its application
    • Kim, L., Mogk, A., and Schumann, W. (1996) A xylose-inducible Bacillus subtilis integration vector and its application. Gene 181, 71-76, 10.1016/S0378-1119(96)00466-0
    • (1996) Gene , vol.181 , pp. 71-76
    • Kim, L.1    Mogk, A.2    Schumann, W.3
  • 12
    • 0030063719 scopus 로고    scopus 로고
    • A T7 promoter-specific, inducible protein expression system forBacillus subtilis
    • Conrad, B., Savchenko, R. S., Breves, R., and Hofemeister, J. (1996) A T7 promoter-specific, inducible protein expression system forBacillus subtilis. Mol. Gen. Genet. 250, 230-236, 10.1007/s004380050071
    • (1996) Mol. Gen. Genet. , vol.250 , pp. 230-236
    • Conrad, B.1    Savchenko, R.S.2    Breves, R.3    Hofemeister, J.4
  • 13
    • 0035163555 scopus 로고    scopus 로고
    • Development and Characterization of a Xylose-Dependent System for Expression of Cloned Genes inBacillus subtilis: Conditional Complementation of a Teichoic Acid Mutant
    • Bhavsar, A. P., Zhao, X., and Brown, E. D. (2001) Development and Characterization of a Xylose-Dependent System for Expression of Cloned Genes inBacillus subtilis: Conditional Complementation of a Teichoic Acid Mutant. Appl. Environ. Microbiol. 67, 403-410, 10.1128/AEM.67.1.403-410.2001
    • (2001) Appl. Environ. Microbiol. , vol.67 , pp. 403-410
    • Bhavsar, A.P.1    Zhao, X.2    Brown, E.D.3
  • 14
    • 29144508806 scopus 로고    scopus 로고
    • Development and Characterization of a Subtilin-Regulated Expression System in Bacillus subtilis: Strict Control of Gene Expression by Addition of Subtilin
    • Bongers, R. S., Veening, J.-W., Wieringen, M. V., Kuipers, O. P., and Kleerebezem, M. (2005) Development and Characterization of a Subtilin-Regulated Expression System in Bacillus subtilis: Strict Control of Gene Expression by Addition of Subtilin. Appl. Environ. Microbiol. 71, 8818-8824, 10.1128/AEM.71.12.8818-8824.2005
    • (2005) Appl. Environ. Microbiol. , vol.71 , pp. 8818-8824
    • Bongers, R.S.1    Veening, J.-W.2    Wieringen, M.V.3    Kuipers, O.P.4    Kleerebezem, M.5
  • 15
    • 77952175929 scopus 로고    scopus 로고
    • Construction of Chromosomally Located T7 Expression System for Production of Heterologous Secreted Proteins in Bacillus subtilis
    • Chen, P. T., Shaw, J.-F., Chao, Y.-P., David Ho, T.-H., and Yu, S.-M. (2010) Construction of Chromosomally Located T7 Expression System for Production of Heterologous Secreted Proteins in Bacillus subtilis. J. Agric. Food Chem. 58, 5392-5399, 10.1021/jf100445a
    • (2010) J. Agric. Food Chem. , vol.58 , pp. 5392-5399
    • Chen, P.T.1    Shaw, J.-F.2    Chao, Y.-P.3    David Ho, T.-H.4    Yu, S.-M.5
  • 16
    • 84867894567 scopus 로고    scopus 로고
    • The LIKE system, a novel protein expression toolbox for Bacillus subtilis based on the liaI promoter
    • Toymentseva, A. A., Schrecke, K., Sharipova, M. R., and Mascher, T. (2012) The LIKE system, a novel protein expression toolbox for Bacillus subtilis based on the liaI promoter. Microb. Cell Fact. 11, 143, 10.1186/1475-2859-11-143
    • (2012) Microb. Cell Fact. , vol.11 , pp. 143
    • Toymentseva, A.A.1    Schrecke, K.2    Sharipova, M.R.3    Mascher, T.4
  • 17
    • 0026305697 scopus 로고
    • Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor
    • Dubendorf, J. W. and Studier, F. W. (1991) Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. J. Mol. Biol. 219, 45-59, 10.1016/0022-2836(91)90856-2
    • (1991) J. Mol. Biol. , vol.219 , pp. 45-59
    • Dubendorf, J.W.1    Studier, F.W.2
  • 18
    • 0029018327 scopus 로고
    • Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
    • Guzman, L. M., Belin, D., Carson, M. J., and Beckwith, J. (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177, 4121-4130, 10.1128/jb.177.14.4121-4130.1995
    • (1995) J. Bacteriol. , vol.177 , pp. 4121-4130
    • Guzman, L.M.1    Belin, D.2    Carson, M.J.3    Beckwith, J.4
  • 19
    • 0030861452 scopus 로고    scopus 로고
    • Independent and Tight Regulation of Transcriptional Units in Escherichia Coli via the LacR/O, the TetR/O and AraC/I1-I2 Regulatory Elements
    • Lutz, R. and Bujard, H. (1997) Independent and Tight Regulation of Transcriptional Units in Escherichia Coli via the LacR/O, the TetR/O and AraC/I1-I2 Regulatory Elements. Nucleic Acids Res. 25, 1203-1210, 10.1093/nar/25.6.1203
    • (1997) Nucleic Acids Res. , vol.25 , pp. 1203-1210
    • Lutz, R.1    Bujard, H.2
  • 20
    • 84975166552 scopus 로고    scopus 로고
    • An extraordinary stringent and sensitive light-switchable gene expression system for bacterial cells
    • Chen, X. et al. (2016) An extraordinary stringent and sensitive light-switchable gene expression system for bacterial cells. Cell Res. 26, 854-857, 10.1038/cr.2016.74
    • (2016) Cell Res. , vol.26 , pp. 854-857
    • Chen, X.1
  • 21
    • 0036720121 scopus 로고    scopus 로고
    • Genome-Wide Analysis of the Stationary-Phase Sigma Factor (Sigma-H) Regulon of Bacillus subtilis
    • Britton, R. A. et al. (2002) Genome-Wide Analysis of the Stationary-Phase Sigma Factor (Sigma-H) Regulon of Bacillus subtilis. J. Bacteriol. 184, 4881-4890, 10.1128/JB.184.17.4881-4890.2002
    • (2002) J. Bacteriol. , vol.184 , pp. 4881-4890
    • Britton, R.A.1
  • 22
    • 30544433196 scopus 로고    scopus 로고
    • Engineering and characterization of a superfolder green fluorescent protein
    • Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C., and Waldo, G. S. (2006) Engineering and characterization of a superfolder green fluorescent protein. Nat. Biotechnol. 24, 79-88, 10.1038/nbt1172
    • (2006) Nat. Biotechnol. , vol.24 , pp. 79-88
    • Pédelacq, J.-D.1    Cabantous, S.2    Tran, T.3    Terwilliger, T.C.4    Waldo, G.S.5
  • 23
    • 84869476328 scopus 로고    scopus 로고
    • Ribozyme-based insulator parts buffer synthetic circuits from genetic context
    • Lou, C., Stanton, B., Chen, Y.-J., Munsky, B., and Voigt, C. A. (2012) Ribozyme-based insulator parts buffer synthetic circuits from genetic context. Nat. Biotechnol. 30, 1137-1142, 10.1038/nbt.2401
    • (2012) Nat. Biotechnol. , vol.30 , pp. 1137-1142
    • Lou, C.1    Stanton, B.2    Chen, Y.-J.3    Munsky, B.4    Voigt, C.A.5
  • 24
    • 84978776996 scopus 로고    scopus 로고
    • FlowCal: A User-Friendly, Open Source Software Tool for Automatically Converting Flow Cytometry Data from Arbitrary to Calibrated Units
    • Castillo-Hair, S. M. et al. (2016) FlowCal: A User-Friendly, Open Source Software Tool for Automatically Converting Flow Cytometry Data from Arbitrary to Calibrated Units. ACS Synth. Biol. 5, 774-780, 10.1021/acssynbio.5b00284
    • (2016) ACS Synth. Biol. , vol.5 , pp. 774-780
    • Castillo-Hair, S.M.1
  • 25
    • 0034750146 scopus 로고    scopus 로고
    • In Vivo Effects of Sporulation Kinases on Mutant Spo0A Proteins in Bacillus subtilis
    • Quisel, J. D., Burkholder, W. F., and Grossman, A. D. (2001) In Vivo Effects of Sporulation Kinases on Mutant Spo0A Proteins in Bacillus subtilis. J. Bacteriol. 183, 6573-6578, 10.1128/JB.183.22.6573-6578.2001
    • (2001) J. Bacteriol. , vol.183 , pp. 6573-6578
    • Quisel, J.D.1    Burkholder, W.F.2    Grossman, A.D.3
  • 26
    • 84921637463 scopus 로고    scopus 로고
    • Density of founder cells affects spatial pattern formation and cooperation in Bacillus subtilis biofilms
    • van Gestel, J., Weissing, F. J., Kuipers, O. P., and Kovács, á. T. (2014) Density of founder cells affects spatial pattern formation and cooperation in Bacillus subtilis biofilms. ISME J. 8, 2069-2079, 10.1038/ismej.2014.52
    • (2014) ISME J. , vol.8 , pp. 2069-2079
    • Van Gestel, J.1    Weissing, F.J.2    Kuipers, O.P.3    Kovács, Á.T.4
  • 27
    • 0023042283 scopus 로고
    • Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
    • Studier, F. W. and Moffatt, B. A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113-130, 10.1016/0022-2836(86)90385-2
    • (1986) J. Mol. Biol. , vol.189 , pp. 113-130
    • Studier, F.W.1    Moffatt, B.A.2
  • 28
    • 33645293152 scopus 로고    scopus 로고
    • An excitable gene regulatory circuit induces transient cellular differentiation
    • Süel, G. M., Garcia-Ojalvo, J., Liberman, L. M., and Elowitz, M. B. (2006) An excitable gene regulatory circuit induces transient cellular differentiation. Nature 440, 545-550, 10.1038/nature04588
    • (2006) Nature , vol.440 , pp. 545-550
    • Süel, G.M.1    Garcia-Ojalvo, J.2    Liberman, L.M.3    Elowitz, M.B.4
  • 29
    • 0023410779 scopus 로고
    • The effect of restriction on shotgun cloning and plasmid stability in Bacillus subtilis Marburg
    • Haima, P., Bron, S., and Venema, G. (1987) The effect of restriction on shotgun cloning and plasmid stability in Bacillus subtilis Marburg. Mol. Gen. Genet. 209, 335-342, 10.1007/BF00329663
    • (1987) Mol. Gen. Genet. , vol.209 , pp. 335-342
    • Haima, P.1    Bron, S.2    Venema, G.3
  • 30
    • 55749116368 scopus 로고    scopus 로고
    • The Origins of 168, W23, and Other Bacillus subtilis Legacy Strains
    • Zeigler, D. R. et al. (2008) The Origins of 168, W23, and Other Bacillus subtilis Legacy Strains. J. Bacteriol. 190, 6983-6995, 10.1128/JB.00722-08
    • (2008) J. Bacteriol. , vol.190 , pp. 6983-6995
    • Zeigler, D.R.1
  • 31
    • 0036192373 scopus 로고    scopus 로고
    • An investigation into the compartmentalization of the sporulation transcription factor σe in Bacillus subtilis
    • Fujita, M. and Losick, R. (2002) An investigation into the compartmentalization of the sporulation transcription factor σE in Bacillus subtilis. Mol. Microbiol. 43, 27-38, 10.1046/j.1365-2958.2002.02732.x
    • (2002) Mol. Microbiol. , vol.43 , pp. 27-38
    • Fujita, M.1    Losick, R.2
  • 32
    • 85045509697 scopus 로고    scopus 로고
    • Phosphatase activity tunes two-component system sensor detection threshold
    • Landry, B. P., Palanki, R., Dyulgyarov, N., Hartsough, L. A., and Tabor, J. J. (2018) Phosphatase activity tunes two-component system sensor detection threshold. Nat. Commun. 9, 1433, 10.1038/s41467-018-03929-y
    • (2018) Nat. Commun. , vol.9 , pp. 1433
    • Landry, B.P.1    Palanki, R.2    Dyulgyarov, N.3    Hartsough, L.A.4    Tabor, J.J.5
  • 33
    • 65849183178 scopus 로고    scopus 로고
    • Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes
    • Engler, C., Gruetzner, R., Kandzia, R., and Marillonnet, S. (2009) Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes. PLoS One 4, e5553 10.1371/journal.pone.0005553
    • (2009) PLoS One , vol.4 , pp. e5553
    • Engler, C.1    Gruetzner, R.2    Kandzia, R.3    Marillonnet, S.4
  • 34
    • 78149355812 scopus 로고    scopus 로고
    • NUPACK: Analysis and design of nucleic acid systems
    • Zadeh, J. N. et al. (2011) NUPACK: Analysis and design of nucleic acid systems. J. Comput. Chem. 32, 170-173, 10.1002/jcc.21596
    • (2011) J. Comput. Chem. , vol.32 , pp. 170-173
    • Zadeh, J.N.1
  • 35
    • 68949163861 scopus 로고    scopus 로고
    • In Vivo Domain-Based Functional Analysis of the Major Sporulation Sensor Kinase, KinA, in Bacillus subtilis
    • Eswaramoorthy, P., Guo, T., and Fujita, M. (2009) In Vivo Domain-Based Functional Analysis of the Major Sporulation Sensor Kinase, KinA, in Bacillus subtilis. J. Bacteriol. 191, 5358-5368, 10.1128/JB.00503-09
    • (2009) J. Bacteriol. , vol.191 , pp. 5358-5368
    • Eswaramoorthy, P.1    Guo, T.2    Fujita, M.3
  • 36
    • 0033998941 scopus 로고    scopus 로고
    • Temporal and selective association of multiple sigma factors with RNA polymerase during sporulation in Bacillus subtilis
    • Fujita, M. (2000) Temporal and selective association of multiple sigma factors with RNA polymerase during sporulation in Bacillus subtilis. Genes Cells 5, 79-88, 10.1046/j.1365-2443.2000.00307.x
    • (2000) Genes Cells , vol.5 , pp. 79-88
    • Fujita, M.1
  • 38
    • 85025070725 scopus 로고    scopus 로고
    • Lmfit: Non-Linear Least-Square Minimization and Curve-Fitting for Python
    • Newville, M., et al., (2016) Lmfit: Non-Linear Least-Square Minimization and Curve-Fitting for Python. In Astrophysics Source Code Library.
    • (2016) Astrophysics Source Code Library
    • Newville, M.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.