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Volumn 11, Issue , 2012, Pages

The LIKE system, a novel protein expression toolbox for Bacillus subtilis based on the liaI promoter

Author keywords

Antibiotic inducible promoter; Bacillus subtilis; Bacitracin; Cell envelope stress response; LiaIH operon; Protein expression; Two component system

Indexed keywords

BACITRACIN; BACTERIAL VECTOR; GREEN FLUORESCENT PROTEIN; PROTEINASE;

EID: 84867894567     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-11-143     Document Type: Article
Times cited : (50)

References (48)
  • 1
    • 1942538348 scopus 로고    scopus 로고
    • Developments in the use of Bacillus species for industrial production
    • 10.1139/w03-076, 15052317
    • Schallmey M, Singh A, Ward OP. Developments in the use of Bacillus species for industrial production. Can J Microbiol 2004, 50:1-17. 10.1139/w03-076, 15052317.
    • (2004) Can J Microbiol , vol.50 , pp. 1-17
    • Schallmey, M.1    Singh, A.2    Ward, O.P.3
  • 5
    • 33845946823 scopus 로고    scopus 로고
    • Protein secretion pathways in Bacillus subtilis: implication for optimization of heterologous protein secretion
    • Ling Lin F, Zi Rong X, Wei Fen L, Jiang Bing S, Ping L, Chun Xia H. Protein secretion pathways in Bacillus subtilis: implication for optimization of heterologous protein secretion. Biotechnol Adv 2007, 25:1-12.
    • (2007) Biotechnol Adv , vol.25 , pp. 1-12
    • Ling Lin, F.1    Zi Rong, X.2    Wei Fen, L.3    Jiang Bing, S.4    Ping, L.5    Chun Xia, H.6
  • 6
    • 8844278305 scopus 로고    scopus 로고
    • Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism
    • 10.1016/j.bbamcr.2004.02.011, 15546673
    • Westers L, Westers H, Quax WJ. Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism. Biochim Biophys Acta 2004, 1694:299-310. 10.1016/j.bbamcr.2004.02.011, 15546673.
    • (2004) Biochim Biophys Acta , vol.1694 , pp. 299-310
    • Westers, L.1    Westers, H.2    Quax, W.J.3
  • 7
    • 34548508428 scopus 로고    scopus 로고
    • Production of recombinant proteins in Bacillus subtilis
    • Schumann W. Production of recombinant proteins in Bacillus subtilis. Adv Appl Microbiol 2007, 62:137-189.
    • (2007) Adv Appl Microbiol , vol.62 , pp. 137-189
    • Schumann, W.1
  • 8
    • 0035163555 scopus 로고    scopus 로고
    • Development and characterization of a xylose-dependent system for expression of cloned genes in Bacillus subtilis: conditional complementation of a teichoic acid mutant
    • 10.1128/AEM.67.1.403-410.2001, 92592, 11133472
    • Bhavsar AP, Zhao X, Brown ED. Development and characterization of a xylose-dependent system for expression of cloned genes in Bacillus subtilis: conditional complementation of a teichoic acid mutant. Appl Environ Microbiol 2001, 67:403-410. 10.1128/AEM.67.1.403-410.2001, 92592, 11133472.
    • (2001) Appl Environ Microbiol , vol.67 , pp. 403-410
    • Bhavsar, A.P.1    Zhao, X.2    Brown, E.D.3
  • 9
    • 29144508806 scopus 로고    scopus 로고
    • Development and characterization of a subtilin-regulated expression system in Bacillus subtilis: strict control of gene expression by addition of subtilin
    • 10.1128/AEM.71.12.8818-8824.2005, 1317459, 16332878
    • Bongers RS, Veening J-W, Van Wieringen M, Kuipers OP, Kleerebezem M. Development and characterization of a subtilin-regulated expression system in Bacillus subtilis: strict control of gene expression by addition of subtilin. Appl Environ Microbiol 2005, 71:8818-8824. 10.1128/AEM.71.12.8818-8824.2005, 1317459, 16332878.
    • (2005) Appl Environ Microbiol , vol.71 , pp. 8818-8824
    • Bongers, R.S.1    Veening, J.-W.2    Van Wieringen, M.3    Kuipers, O.P.4    Kleerebezem, M.5
  • 10
    • 0030063719 scopus 로고    scopus 로고
    • A T7 promoter-specific, inducible protein expression system for Bacillus subtilis
    • Conrad B, Savchenko RS, Breves R, Hofemeister J. A T7 promoter-specific, inducible protein expression system for Bacillus subtilis. Mol Gen Genet 1996, 250:230-236.
    • (1996) Mol Gen Genet , vol.250 , pp. 230-236
    • Conrad, B.1    Savchenko, R.S.2    Breves, R.3    Hofemeister, J.4
  • 11
    • 77955417593 scopus 로고    scopus 로고
    • Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis
    • 10.1016/j.jbiotec.2010.06.021, 20600378
    • Lee SJ, Pan JG, Park SH, Choi SK. Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis. J Biotechnol 2010, 149:16-20. 10.1016/j.jbiotec.2010.06.021, 20600378.
    • (2010) J Biotechnol , vol.149 , pp. 16-20
    • Lee, S.J.1    Pan, J.G.2    Park, S.H.3    Choi, S.K.4
  • 12
    • 1542345030 scopus 로고    scopus 로고
    • An efficient heat-inducible Bacillus subtilis bacteriophage 105 expression and secretion system for the production of the Streptomyces clavuligerus beta-lactamase inhibitory protein (BLIP)
    • 10.1016/j.jbiotec.2003.12.004, 15006422
    • Liu HB, Chui KS, Chan CL, Tsang CW, Leung YC. An efficient heat-inducible Bacillus subtilis bacteriophage 105 expression and secretion system for the production of the Streptomyces clavuligerus beta-lactamase inhibitory protein (BLIP). J Biotechnol 2004, 108:207-217. 10.1016/j.jbiotec.2003.12.004, 15006422.
    • (2004) J Biotechnol , vol.108 , pp. 207-217
    • Liu, H.B.1    Chui, K.S.2    Chan, C.L.3    Tsang, C.W.4    Leung, Y.C.5
  • 13
    • 33947367749 scopus 로고    scopus 로고
    • A novel cold-inducible expression system for Bacillus subtilis
    • 10.1016/j.pep.2006.12.023, 17307364
    • Le Thuy AT, Schumann W. A novel cold-inducible expression system for Bacillus subtilis. Protein Expr Purif 2007, 53:264-269. 10.1016/j.pep.2006.12.023, 17307364.
    • (2007) Protein Expr Purif , vol.53 , pp. 264-269
    • Le Thuy, A.T.1    Schumann, W.2
  • 14
    • 80052802790 scopus 로고    scopus 로고
    • Self-inducible Bacillus subtilis expression system for reliable and inexpensive protein production by high-cell-density fermentation
    • 10.1128/AEM.05219-11, 3187127, 21803899
    • Wenzel M, Müller A, Siemann-Herzberg M, Altenbuchner J. Self-inducible Bacillus subtilis expression system for reliable and inexpensive protein production by high-cell-density fermentation. Appl Environ Microbiol 2011, 77:6419-6425. 10.1128/AEM.05219-11, 3187127, 21803899.
    • (2011) Appl Environ Microbiol , vol.77 , pp. 6419-6425
    • Wenzel, M.1    Müller, A.2    Siemann-Herzberg, M.3    Altenbuchner, J.4
  • 15
    • 27544451339 scopus 로고    scopus 로고
    • 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis
    • 10.1007/s00253-005-0107-6, 16088349
    • Mierau I, Kleerebezem M. 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. Appl Microbiol Biotechnol 2005, 68:705-717. 10.1007/s00253-005-0107-6, 16088349.
    • (2005) Appl Microbiol Biotechnol , vol.68 , pp. 705-717
    • Mierau, I.1    Kleerebezem, M.2
  • 16
    • 33646154448 scopus 로고    scopus 로고
    • Construction and characterization of nisin-controlled expression vectors for use in Lactobacillus reuteri
    • 10.1271/bbb.70.757, 16636439
    • Wu CM, Lin CF, Chang YC, Chung TC. Construction and characterization of nisin-controlled expression vectors for use in Lactobacillus reuteri. Biosci Biotechnol Biochem 2006, 70:757-767. 10.1271/bbb.70.757, 16636439.
    • (2006) Biosci Biotechnol Biochem , vol.70 , pp. 757-767
    • Wu, C.M.1    Lin, C.F.2    Chang, Y.C.3    Chung, T.C.4
  • 17
    • 78649866559 scopus 로고    scopus 로고
    • Comparison of different Bacillus subtilis expression systems
    • 10.1016/j.resmic.2010.09.004, 20863884
    • Vavrova L, Muchova K, Barak I. Comparison of different Bacillus subtilis expression systems. Res Microbiol 2010, 161:791-797. 10.1016/j.resmic.2010.09.004, 20863884.
    • (2010) Res Microbiol , vol.161 , pp. 791-797
    • Vavrova, L.1    Muchova, K.2    Barak, I.3
  • 18
    • 0348147592 scopus 로고    scopus 로고
    • Cell wall stress responses in Bacillus subtilis: the regulatory network of the bacitracin stimulon
    • 10.1046/j.1365-2958.2003.03786.x, 14651641
    • Mascher T, Margulis NG, Wang T, Ye RW, Helmann JD. Cell wall stress responses in Bacillus subtilis: the regulatory network of the bacitracin stimulon. Mol Microbiol 2003, 50:1591-1604. 10.1046/j.1365-2958.2003.03786.x, 14651641.
    • (2003) Mol Microbiol , vol.50 , pp. 1591-1604
    • Mascher, T.1    Margulis, N.G.2    Wang, T.3    Ye, R.W.4    Helmann, J.D.5
  • 19
    • 37349034233 scopus 로고    scopus 로고
    • Cell envelope stress response in Gram-positive bacteria
    • 10.1111/j.1574-6976.2007.00091.x, 18173394
    • Jordan S, Hutchings MI, Mascher T. Cell envelope stress response in Gram-positive bacteria. FEMS Microbiol Rev 2008, 32:107-146. 10.1111/j.1574-6976.2007.00091.x, 18173394.
    • (2008) FEMS Microbiol Rev , vol.32 , pp. 107-146
    • Jordan, S.1    Hutchings, M.I.2    Mascher, T.3
  • 20
    • 84868013029 scopus 로고    scopus 로고
    • Two-component signaling in the Gram-positive envelope stress response: intramembrane-sensing histidine kinases and accessory membrane proteins
    • UK: Horizon Scientific Press, Gross R, Beier D, Hethersett N
    • Schrecke K, Staroń A, Mascher T. Two-component signaling in the Gram-positive envelope stress response: intramembrane-sensing histidine kinases and accessory membrane proteins. Two component systems in bacteria 2012, UK: Horizon Scientific Press, Gross R, Beier D, Hethersett N.
    • (2012) Two component systems in bacteria
    • Schrecke, K.1    Staroń, A.2    Mascher, T.3
  • 21
    • 33745923792 scopus 로고    scopus 로고
    • Regulation of LiaRS-dependent gene expression in Bacillus subtilis: Identification of inhibitor proteins, regulator binding sites and target genes of a conserved cell envelope stress-sensing two-component system
    • 10.1128/JB.00310-06, 1539951, 16816187
    • Jordan S, Junker A, Helmann JD, Mascher T. Regulation of LiaRS-dependent gene expression in Bacillus subtilis: Identification of inhibitor proteins, regulator binding sites and target genes of a conserved cell envelope stress-sensing two-component system. J Bacteriol 2006, 188:5153-5166. 10.1128/JB.00310-06, 1539951, 16816187.
    • (2006) J Bacteriol , vol.188 , pp. 5153-5166
    • Jordan, S.1    Junker, A.2    Helmann, J.D.3    Mascher, T.4
  • 22
    • 3342908566 scopus 로고    scopus 로고
    • Antibiotic-inducible promoter regulated by the cell envelope stress-sensing two-component system LiaRS of Bacillus subtilis
    • 10.1128/AAC.48.8.2888-2896.2004, 478541, 15273097
    • Mascher T, Zimmer SL, Smith TA, Helmann JD. Antibiotic-inducible promoter regulated by the cell envelope stress-sensing two-component system LiaRS of Bacillus subtilis. Antimicrob Agents Chemother 2004, 48:2888-2896. 10.1128/AAC.48.8.2888-2896.2004, 478541, 15273097.
    • (2004) Antimicrob Agents Chemother , vol.48 , pp. 2888-2896
    • Mascher, T.1    Zimmer, S.L.2    Smith, T.A.3    Helmann, J.D.4
  • 24
    • 48749083048 scopus 로고    scopus 로고
    • Microtiter plate bioassay to monitor the interference of antibiotics with the lipid II cycle essential for peptidoglycan biosynthesis
    • 10.1016/j.mimet.2008.05.002, 18558445
    • Burkard M, Stein T. Microtiter plate bioassay to monitor the interference of antibiotics with the lipid II cycle essential for peptidoglycan biosynthesis. J Microbiol Methods 2008, 75:70-74. 10.1016/j.mimet.2008.05.002, 18558445.
    • (2008) J Microbiol Methods , vol.75 , pp. 70-74
    • Burkard, M.1    Stein, T.2
  • 25
    • 78751685410 scopus 로고    scopus 로고
    • Peptide antibiotic sensing and detoxification modules of Bacillus subtilis
    • 10.1128/AAC.00352-10, 3028804, 21078927
    • Staroń A, Finkeisen DE, Mascher T. Peptide antibiotic sensing and detoxification modules of Bacillus subtilis. Antimicrob Agents Chemother 2011, 55:515-525. 10.1128/AAC.00352-10, 3028804, 21078927.
    • (2011) Antimicrob Agents Chemother , vol.55 , pp. 515-525
    • Staroń, A.1    Finkeisen, D.E.2    Mascher, T.3
  • 26
    • 0036786891 scopus 로고    scopus 로고
    • Correlations between Shine-Dalgarno sequences and gene features such as predicted expression levels and operon structures
    • 10.1128/JB.184.20.5733-5745.2002, 139613, 12270832
    • Ma J, Campbell A, Karlin S. Correlations between Shine-Dalgarno sequences and gene features such as predicted expression levels and operon structures. J Bacteriol 2002, 184:5733-5745. 10.1128/JB.184.20.5733-5745.2002, 139613, 12270832.
    • (2002) J Bacteriol , vol.184 , pp. 5733-5745
    • Ma, J.1    Campbell, A.2    Karlin, S.3
  • 27
    • 0026544545 scopus 로고
    • The influence of ribosome-binding-site elements on translational efficiency in Bacillus subtilis and Escherichia coli in vivo
    • 10.1111/j.1365-2958.1992.tb01548.x, 1375309
    • Vellanoweth RL, Rabinowitz JC. The influence of ribosome-binding-site elements on translational efficiency in Bacillus subtilis and Escherichia coli in vivo. Mol Microbiol 1992, 6:1105-1114. 10.1111/j.1365-2958.1992.tb01548.x, 1375309.
    • (1992) Mol Microbiol , vol.6 , pp. 1105-1114
    • Vellanoweth, R.L.1    Rabinowitz, J.C.2
  • 28
    • 0030597337 scopus 로고    scopus 로고
    • Plasmids for ectopic integration in Bacillus subtilis
    • 10.1016/S0378-1119(96)00404-0, 8973347
    • Guerout-Fleury AM, Frandsen N, Stragier P. Plasmids for ectopic integration in Bacillus subtilis. Gene 1996, 180:57-61. 10.1016/S0378-1119(96)00404-0, 8973347.
    • (1996) Gene , vol.180 , pp. 57-61
    • Guerout-Fleury, A.M.1    Frandsen, N.2    Stragier, P.3
  • 29
    • 36549032156 scopus 로고    scopus 로고
    • SPINE: a method for the rapid detection and analysis of protein-protein interactions in vivo
    • 10.1002/pmic.200700491, 17994626
    • Herzberg C, Weidinger LA, Dörrbecker B, Hübner S, Stülke J, Commichau FM. SPINE: a method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 2007, 7:4032-4035. 10.1002/pmic.200700491, 17994626.
    • (2007) Proteomics , vol.7 , pp. 4032-4035
    • Herzberg, C.1    Weidinger, L.A.2    Dörrbecker, B.3    Hübner, S.4    Stülke, J.5    Commichau, F.M.6
  • 30
    • 45549097452 scopus 로고    scopus 로고
    • Positive feedback in cellular control systems
    • 10.1002/bies.20769, 2486260, 18478531
    • Mitrophanov AY, Groisman EA. Positive feedback in cellular control systems. BioEssays 2008, 30:542-555. 10.1002/bies.20769, 2486260, 18478531.
    • (2008) BioEssays , vol.30 , pp. 542-555
    • Mitrophanov, A.Y.1    Groisman, E.A.2
  • 31
    • 0029973636 scopus 로고    scopus 로고
    • FACS-optimized mutants of the green fluorescent protein (GFP)
    • 10.1016/0378-1119(95)00685-0, 8707053
    • Cormack BP, Valdivia RH, Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 1996, 173:33-38. 10.1016/0378-1119(95)00685-0, 8707053.
    • (1996) Gene , vol.173 , pp. 33-38
    • Cormack, B.P.1    Valdivia, R.H.2    Falkow, S.3
  • 32
    • 41749088673 scopus 로고    scopus 로고
    • Bacitracin sensing in Bacillus subtilis
    • 10.1111/j.1365-2958.2008.06194.x, 18394148
    • Rietkötter E, Hoyer D, Mascher T. Bacitracin sensing in Bacillus subtilis. Mol Microbiol 2008, 68:768-785. 10.1111/j.1365-2958.2008.06194.x, 18394148.
    • (2008) Mol Microbiol , vol.68 , pp. 768-785
    • Rietkötter, E.1    Hoyer, D.2    Mascher, T.3
  • 33
    • 0142231968 scopus 로고    scopus 로고
    • Structure and function of "metalloantibiotics"
    • 10.1002/med.10052, 12939790
    • Ming LJ. Structure and function of "metalloantibiotics". Med Res Rev 2003, 23:697-762. 10.1002/med.10052, 12939790.
    • (2003) Med Res Rev , vol.23 , pp. 697-762
    • Ming, L.J.1
  • 34
    • 0034931152 scopus 로고    scopus 로고
    • Alkaline shock induces the Bacillus subtilis σW regulon
    • 10.1046/j.1365-2958.2001.02489.x, 11454200
    • Wiegert T, Homuth G, Versteeg S, Schumann W. Alkaline shock induces the Bacillus subtilis σW regulon. Mol Microbiol 2001, 41:59-71. 10.1046/j.1365-2958.2001.02489.x, 11454200.
    • (2001) Mol Microbiol , vol.41 , pp. 59-71
    • Wiegert, T.1    Homuth, G.2    Versteeg, S.3    Schumann, W.4
  • 35
    • 84873523437 scopus 로고    scopus 로고
    • Extracytoplasmic function sigma factors of the widely distributed group ECF41 contain a fused regulatory domain
    • 10.1002/mbo3.22, 3426412, 22950025
    • Wecke T, Halang P, Staroń A, Dufour YS, Donohue TJ, Mascher T. Extracytoplasmic function sigma factors of the widely distributed group ECF41 contain a fused regulatory domain. Microbiology Open 2012, 1:194-213. 10.1002/mbo3.22, 3426412, 22950025.
    • (2012) Microbiology Open , vol.1 , pp. 194-213
    • Wecke, T.1    Halang, P.2    Staroń, A.3    Dufour, Y.S.4    Donohue, T.J.5    Mascher, T.6
  • 36
    • 0034810821 scopus 로고    scopus 로고
    • Global analysis of the general stress response of Bacillus subtilis
    • 10.1128/JB.183.19.5617-5631.2001, 95453, 11544224
    • Petersohn A, Brigulla M, Haas S, Hoheisel JD, Völker U, Hecker M. Global analysis of the general stress response of Bacillus subtilis. J Bacteriol 2001, 183:5617-5631. 10.1128/JB.183.19.5617-5631.2001, 95453, 11544224.
    • (2001) J Bacteriol , vol.183 , pp. 5617-5631
    • Petersohn, A.1    Brigulla, M.2    Haas, S.3    Hoheisel, J.D.4    Völker, U.5    Hecker, M.6
  • 37
    • 19044379164 scopus 로고    scopus 로고
    • Cationic antimicrobial peptides elicit a complex stress response in Bacillus subtilis that involves ECF-type sigma factors and two-component signal transduction systems
    • 10.1099/mic.0.27761-0, 15870467
    • Pietiäinen M, Gardemeister M, Mecklin M, Leskela S, Sarvas M, Kontinen VP. Cationic antimicrobial peptides elicit a complex stress response in Bacillus subtilis that involves ECF-type sigma factors and two-component signal transduction systems. Microbiology 2005, 151:1577-1592. 10.1099/mic.0.27761-0, 15870467.
    • (2005) Microbiology , vol.151 , pp. 1577-1592
    • Pietiäinen, M.1    Gardemeister, M.2    Mecklin, M.3    Leskela, S.4    Sarvas, M.5    Kontinen, V.P.6
  • 38
    • 33745765425 scopus 로고    scopus 로고
    • Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis
    • 10.1111/j.1462-2920.2006.01034.x, 16872404
    • le Tam T, Eymann C, Albrecht D, Sietmann R, Schauer F, Hecker M, Antelmann H. Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis. Environ Microbiol 2006, 8:1408-1427. 10.1111/j.1462-2920.2006.01034.x, 16872404.
    • (2006) Environ Microbiol , vol.8 , pp. 1408-1427
    • le Tam, T.1    Eymann, C.2    Albrecht, D.3    Sietmann, R.4    Schauer, F.5    Hecker, M.6    Antelmann, H.7
  • 39
    • 84861334452 scopus 로고    scopus 로고
    • Comparative transcriptional analysis of Bacillus subtilis cells overproducing either secreted proteins, lipoproteins or membrane proteins
    • 10.1186/1475-2859-11-66, 3514339, 22624725
    • Marciniak BC, Trip H, Kuipers OP, Van-der Veek PJ. Comparative transcriptional analysis of Bacillus subtilis cells overproducing either secreted proteins, lipoproteins or membrane proteins. Microb Cell Fact 2012, 11:66. 10.1186/1475-2859-11-66, 3514339, 22624725.
    • (2012) Microb Cell Fact , vol.11 , pp. 66
    • Marciniak, B.C.1    Trip, H.2    Kuipers, O.P.3    Van-der Veek, P.J.4
  • 40
    • 0018800089 scopus 로고
    • A rapid alkaline extraction procedure for screening recombinant plasmid DNA
    • 10.1093/nar/7.6.1513, 342324, 388356
    • Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979, 7:1513-1523. 10.1093/nar/7.6.1513, 342324, 388356.
    • (1979) Nucleic Acids Res , vol.7 , pp. 1513-1523
    • Birnboim, H.C.1    Doly, J.2
  • 43
    • 8744309111 scopus 로고    scopus 로고
    • New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria
    • 10.1128/AEM.70.11.6887-6891.2004, 525206, 15528558
    • Arnaud M, Chastanet A, Debarbouille M. New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria. Appl Environ Microbiol 2004, 70:6887-6891. 10.1128/AEM.70.11.6887-6891.2004, 525206, 15528558.
    • (2004) Appl Environ Microbiol , vol.70 , pp. 6887-6891
    • Arnaud, M.1    Chastanet, A.2    Debarbouille, M.3
  • 44
    • 0033521857 scopus 로고    scopus 로고
    • GFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis
    • 10.1016/S0378-1119(98)00580-0, 9931458
    • Lewis PJ, Marston AL. GFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis. Gene 1999, 227:101-110. 10.1016/S0378-1119(98)00580-0, 9931458.
    • (1999) Gene , vol.227 , pp. 101-110
    • Lewis, P.J.1    Marston, A.L.2
  • 45
    • 77953223755 scopus 로고    scopus 로고
    • PBaSysBioII: an integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression in Bacillus subtilis
    • 10.1099/mic.0.035758-0, 20150235
    • Botella E, Fogg M, Jules M, Piersma S, Doherty G, Hansen A, Denham EL, Le Chat L, Veiga P, Bailey K, et al. pBaSysBioII: an integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression in Bacillus subtilis. Microbiology 2010, 156:1600-1608. 10.1099/mic.0.035758-0, 20150235.
    • (2010) Microbiology , vol.156 , pp. 1600-1608
    • Botella, E.1    Fogg, M.2    Jules, M.3    Piersma, S.4    Doherty, G.5    Hansen, A.6    Denham, E.L.7    Le Chat, L.8    Veiga, P.9    Bailey, K.10
  • 46
    • 34547881586 scopus 로고    scopus 로고
    • LiaRS-dependent gene expression is embedded in transition state regulation in Bacillus subtilis
    • 10.1099/mic.0.2007/006817-0, 17660417
    • Jordan S, Rietkötter E, Strauch MA, Kalamorz F, Butcher BG, Helmann JD, Mascher T. LiaRS-dependent gene expression is embedded in transition state regulation in Bacillus subtilis. Microbiology 2007, 153:2530-2540. 10.1099/mic.0.2007/006817-0, 17660417.
    • (2007) Microbiology , vol.153 , pp. 2530-2540
    • Jordan, S.1    Rietkötter, E.2    Strauch, M.A.3    Kalamorz, F.4    Butcher, B.G.5    Helmann, J.D.6    Mascher, T.7
  • 47
    • 0023472472 scopus 로고
    • Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
    • 10.1016/0003-2697(87)90587-2, 2449095
    • Schägger H, von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 1987, 166:368-379. 10.1016/0003-2697(87)90587-2, 2449095.
    • (1987) Anal Biochem , vol.166 , pp. 368-379
    • Schägger, H.1    von Jagow, G.2
  • 48
    • 0023931883 scopus 로고
    • Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250
    • 10.1002/elps.1150090603, 2466658
    • Neuhoff V, Arold N, Taube D, Ehrhardt W. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 1988, 9:255-262. 10.1002/elps.1150090603, 2466658.
    • (1988) Electrophoresis , vol.9 , pp. 255-262
    • Neuhoff, V.1    Arold, N.2    Taube, D.3    Ehrhardt, W.4


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