Engineering of a Bacillus subtilis strain with adjustable levels of intracellular biotin for secretory production of functional streptavidin

Applied and Environmental Microbiology

Volumn 68, Issue 3, 2002, Pages 1102-1108

  Wu, Sau Ching ^a   Wong, Sui Lam ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CELL CULTURE; CHROMOSOMES; PROTEINS;

BINDING SITES;

MICROBIOLOGY;

BIOTIN; STREPTAVIDIN;

MICROBIOLOGY;

ARTICLE; BACILLUS SUBTILIS; BACTERIUM CULTURE; BIOENGINEERING; CELL SECRETION; CULTURE MEDIUM; NONHUMAN; PROTEIN SECRETION; PROTEIN SYNTHESIS;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; BIOTIN; CHAPERONINS; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; GLUCONATES; HEAT-SHOCK PROTEINS; OPERATOR REGIONS (GENETICS); PROMOTER REGIONS (GENETICS); STREPTAVIDIN;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 0036199577     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.3.1102-1108.2002     Document Type: Article

References (35)

1. Improvement of production, assay and purification of streptavidin
2. Adsorption of avidin on microfabricated surfaces for protein biochip applications
3. Avidin column as a highly efficient and stable alternative for immobilization of ligands for affinity chromatography
4. Delivery of peptides and proteins through the blood-brain barrier
5. Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon
6. Protein and antibody arrays and their medical applications
7. Production of streptavidin in a synthetic medium
8. Molecular design, expression, and affinity immobilization of a trypsinstreptavidin fusion protein
9. Affinities of different proteins and peptides for lipopolysaccharide as determined by biosensor technology
10. Electrospray ionization mass spectrometry of biotin binding to streptavidin
11. The gluconate operon, gnt, of Bacillus subtilis encodes its own transcriptional repressor
12. Identification of an operator sequence for the Bacillus subtilis gnt operon
13. Production of a soluble and functional recombinant streptavidin in Escherichia coli
14. Calculation of protein extinction coefficients from amino acid sequence data
15. Inducible expression of regulatory genes in Bacillus subtilis
16. Construction of vectors with the p15a replicon, kanamycin resistance, inducible lacZα and pUC18 or pUC19 multiple cloning sites
17. Catabolite repression of the Bacillus subtilis xyl operon involves a cis element functional in the context of an unrelated sequence, and glucose exerts additional xylRdependent repression
18. Regulated promoter for high-level expression of heterologous genes in Bacillus subtilis
19. Characterization of the rate-limiting step of the secretion of Bacillus subtilis alpha-amylase overproduced during the exponential phase of growth
20. Cloning and characterization of the groESL operon from Bacillus subtilis
21. The genes encoding the biotin carboxyl carrier protein and biotin carboxylase subunits of Bacillus subtilis acetyl coenzyme a carboxylase, the first enzyme of fatty acid synthesis
22. Secretion of streptavidin from Bacillus subtilis
23. Sporulation, germination and outgrowth
24. Antibody-guided three-step therapy for high grade glioma with yttrium-90 biotin
25. Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis
26. Efficient utilization of Escherichia coli transcriptional signals in Bacillus subtilis
27. Biotin uptake: Influx, efflux and countertransport in Escherichia coli K12
28. Avidin-biotin system for delivery of diagnostic agents
29. Expression of a cloned streptavidin gene in Escherichia coli
30. Cloning and expression of a novel protease gene encoding an extracellular neutral protease from Bacillus subtilis
31. The avidin-biotin complex in bioanalytical applications
32. Secretory production and purification of functional full-length streptavidin from Bacillus subtilis
33. Functional production and characterization of a fibrin specific single-chain antibody fragment from Bacillus subtilis
34. Transcriptional regulation of the Bacillus subtilis glucitol dehydrogenase gene
35. Construction of protease deficient Bacillus subtilis strains for expression studies: Inactivation of seven extracellular proteases and the intracellular LonA protease