Multicenter evaluation of the eplex respiratory pathogen panel for the detection of viral and bacterial respiratory tract pathogens in nasopharyngeal swabs

Journal of Clinical Microbiology

Volumn 56, Issue 2, 2018, Pages

  Babady, N Esther ^a   England, Matthew R ^b   Smith, Kristen L Jurcic ^c   He, Taojun ^a,d   Wijetunge, Dona Saumya ^b   Tang, Yi Wei ^a,e   Chamberland, Robin R ^f   Menegus, Marilyn ^c   Swierkosz, Ella M ^f   Jerris, Robert C ^g   Greene, Wallace ^b  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b PENN STATE MILTON S HERSHEY MEDICAL CENTER   (United States)

^c UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^d SUN YAT SEN UNIVERSITY   (China)

^e WEILL CORNELL MEDICAL COLLEGE   (United States)

^f SAINT LOUIS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^g CHILDREN'S HEALTHCARE OF ATLANTA   (United States)

Author keywords

Multiplex syndromic panel; Rapid diagnosis; Rapid PCR; Respiratory pathogens; Respiratory tract infections; Sample to answer test

Indexed keywords

BACTERIUM; CLINICAL TRIAL; DIAGNOSTIC TEST; EVALUATION STUDY; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR DIAGNOSIS; MULTICENTER STUDY; NASOPHARYNX; POLYMERASE CHAIN REACTION; PROCEDURES; REPRODUCIBILITY; RESPIRATORY TRACT INFECTION; RETROSPECTIVE STUDY; VIROLOGY; VIRUS;

BACTERIA; DIAGNOSTIC TESTS, ROUTINE; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; NASOPHARYNX; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RESPIRATORY TRACT INFECTIONS; RETROSPECTIVE STUDIES; VIRUSES;

EID: 85066862593     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01658-17     Document Type: Article

References (27)

1. Ferkol T, Schraufnagel D. 2014. The global burden of respiratory disease. Ann Am Thorac Soc 11:404 - 406. https://doi.org/10.1513/AnnalsATS.201311-405PS.
2. Nichols WG, Campbell AJP, Boeckh M. 2008. Respiratory viruses other than influenza virus: Impact and therapeutic advances. Clin Microbiol Rev 21:274 -290. https://doi.org/10.1128/CMR.00045-07.
3. Barlam TF, Soria-Saucedo R, Cabral HJ, Kazis LE. 2016. Unnecessary antibiotics for acute respiratory tract infections: Association with care setting and patient demographics. Open Forum Infect Dis 3:ofw045. https://doi.org/10.1093/ofid/ofw045.
4. Boeckh M. 2008. The challenge of respiratory virus infections in hematopoietic cell transplant recipients. Br J Haematol 143:455- 467. https://doi.org/10.1111/j.1365-2141.2008.07295.x.
5. Talbot HK, Falsey AR. 2010. The diagnosis of viral respiratory disease in older adults. Clin Infect Dis 50:747-751. https://doi.org/10.1086/650486.
6. Walter JM, Wunderink RG. 2017. Severe respiratory viral infections: new evidence and changing paradigms. Infect Dis Clin North Am 31:455- 474. https://doi.org/10.1016/j.idc.2017.05.004.
7. Babady NE. 2016. Hospital-associated infections. Microbiol Spectr 4:DMIH2-0003-2015. https://doi.org/10.1128/microbiolspec.DMIH2-0003-2015.
8. Siegel JD, Rhinehart E, Jackson M, Chiarello L, Health Care Infection Control Practices Advisory Committee. 2007. 2007 Guideline for isolation precautions: Preventing transmission of infectious agents in health care settings. Am J Infect Control 35:S65-S164. https://doi.org/10.1016/j.ajic.2007.10.007.
9. Hanson KE, Couturier MR. 2016. Multiplexed molecular diagnostics for respiratory, gastrointestinal, and central nervous system infections. Clin Infect Dis 63:1361-1367. https://doi.org/10.1093/cid/ciw494.
10. Nijhuis RHT, Guerendiain D, Claas ECJ, Templeton KE. 2017. Comparison of ePlex respiratory pathogen panel with laboratory-developed real-time PCR assays for detection of respiratory pathogens. J Clin Microbiol 55:1938 -1945. https://doi.org/10.1128/JCM.00221-17.
11. Pierce VM, Hodinka RL. 2012. Comparison of the GenMark Diagnostics eSensor respiratory viral panel to real-time PCR for detection of respiratory viruses in children. J Clin Microbiol 50:3458 -3465. https://doi.org/10.1128/JCM.01384-12.
12. BioFire. 2017. FilmArray Respiratory Panel (RP): Instruction booklet. Bio-Fire, Salt Lake City, UT.
13. Food and Drug Administration. 2007. Statistical guidance on reporting results from studies evaluating diagnostic tests. Food and Drug Administration, Washington, DC.
14. Song E, Wang H, Salamon D, Jaggi P, Leber A. 2016. Performance characteristics of FilmArray Respiratory Panel v1.7 for detection of adenovirus in a large cohort of pediatric nasopharyngeal samples: One test may not fit all. J Clin Microbiol 54:1479 -1486. https://doi.org/10.1128/JCM.00143-16.
15. Lepiller Q, Barth H, Lefebvre F, Herbrecht R, Lutz P, Kessler R, Fafi-Kremer S, Stoll-Keller F. 2013. High incidence but low burden of coronaviruses and preferential associations between respiratory viruses. J Clin Microbiol 51:3039 -3046. https://doi.org/10.1128/JCM.01078-13.
16. Royston L, Tapparel C. 2016. Rhinoviruses and respiratory enteroviruses: not as simple as ABC. Viruses 8:E16. https://doi.org/10.3390/v8010016.
17. Davlin SL, Blanton L, Kniss K, Mustaquim D, Smith S, Kramer N, Cohen J, Cummings CN, Garg S, Flannery B, Fry AM, Grohskopf LA, Bresee J, Wallis T, Sessions W, Garten R, Xu X, Elal AI, Gubareva L, Barnes J, Wentworth DE, Burns E, Katz J, Jernigan D, Brammer L. 2016. Influenza activity- United States, 2015-16 season and composition of the 2016-17 influenza vaccine. MMWR Morb Mortal Wkly Rep 65:567-575. https://doi.org/10.15585/mmwr.mm6522a3.
18. Esposito S, Blasi F, Bellini F, Allegra L, Principi N, Mowgli Study Group. 2001. Mycoplasma pneumoniae and Chlamydia pneumoniae infections in children with pneumonia. Mowgli Study Group. Eur Respir J 17: 241-245. https://doi.org/10.1183/09031936.01.17202410.
19. Kline JM, Lewis WD, Smith EA, Tracy LR, Moerschel SK. 2013. Pertussis: A reemerging infection. Am Fam Physician 88:507-514.
20. Jerris RC, Williams SR, MacDonald HJ, Ingebrigtsen DR, Westblade LF, Rogers BB. 2015. Testing implications of varying targets for Bordetella pertussis: Comparison of the FilmArray Respiratory Panel and the Focus B. pertussis PCR assay. J Clin Pathol 68:394 -396. https://doi.org/10.1136/jclinpath-2014-202833.
21. Voiriot G, Visseaux B, Cohen J, Nguyen LB, Neuville M, Morbieu C, Burdet C, Radjou A, Lescure FX, Smonig R, Armand-Lefevre L, Mourvillier B, Yazdanpanah Y, Soubirou JF, Ruckly S, Houhou-Fidouh N, Timsit JF. 2016. Viral-bacterial coinfection affects the presentation and alters the prognosis of severe community-acquired pneumonia. Crit Care 20: 375-383. https://doi.org/10.1186/s13054-016-1517-9.
22. Jacobs SE, Soave R, Shore TB, Satlin MJ, Schuetz AN, Magro C, Jenkins SG, Walsh TJ. 2013. Human rhinovirus infections of the lower respiratory tract in hematopoietic stem cell transplant recipients. Transpl Infect Dis 15:474-486. https://doi.org/10.1111/tid.12111.
23. Seo S, Waghmare A, Scott EM, Xie H, Kuypers JM, Hackman RC, Campbell AP, Choi SM, Leisenring WM, Jerome KR, Englund JA, Boeckh M. 2017. Human rhinovirus detection in the lower respiratory tract of hematopoietic cell transplant recipients: Association with mortality. Haematologicaae 102: 1120-1130. https://doi.org/10.3324/haematol.2016.153767.
24. Butt SA, Maceira VP, McCallen ME, Stellrecht KA. 2014. Comparison of three commercial RT-PCR systems for the detection of respiratory viruses. J Clin Virol 61:406-410. https://doi.org/10.1016/j.jcv.2014.08.010.
25. Juretschko S, Mahony J, Buller RS, Manji R, Dunbar S, Walker K, Rao A. 2017. Multicenter clinical evaluation of the Luminex Aries Flu A/B & RSV assay for pediatric and adult respiratory tract specimens. J Clin Microbiol 55:2431-2438. https://doi.org/10.1128/JCM.00318-17.
26. Loeffelholz MJ, Pong DL, Pyles RB, Xiong Y, Miller AL, Bufton KK, Chonmaitree T. 2011. Comparison of the FilmArray Respiratory Panel and Prodesse real-time PCR assays for detection of respiratory pathogens. J Clin Microbiol 49:4083- 4088. https://doi.org/10.1128/JCM.05010-11.
27. Rand KH, Rampersaud H, Houck HJ. 2011. Comparison of two multiplex methods for detection of respiratory viruses: FilmArray RP and xTAG RVP. J Clin Microbiol 49:2449-2453. https://doi.org/10.1128/JCM.02582-10.